LOCUS       sequence-469018-        9659 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral expression of human Delta-37-PPM1H with C-terminal mito 
            signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9659)
  AUTHORS   Wondwossen M Yeshaw , Ayan Adhikari, Claire Y Chiang, Herschel S 
            Dhekne, Paulina S Wawro, Suzanne R Pfeffer
  TITLE     Localization of PPM1H phosphatase tunes Parkinson's disease-linked 
            LRRK2 kinase-mediated Rab GTPase phosphorylation and ciliogenesis
  JOURNAL   Proc Natl Acad Sci U S A. 2023 Oct 31;120(44)
REFERENCE   2  (bases 1 to 9659)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2023 Oct 31;120(44)"
COMMENT     Sequence Label: Lenti-TO-HA-Delta-37 PPM1H-mito
FEATURES             Location/Qualifiers
     source          1..9659
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(84..128)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    313..546
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1039..1164
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             1211..1391
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     promoter        complement(1392..1618)
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     primer_bind     complement(1633..1650)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1633..1649)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1642..1664)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1858..1877)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1977..1999
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(2037..2055)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2123..2227
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2228..2296,2297..3088)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2446..2465)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3259..3847
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4001..4018
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4135..4156
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4171..4176,4177..4194,4195..4201)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4209..4225
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4214..4236
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4233..4249
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4233..4249
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      4282..4417
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    4457..4578
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4468..4487)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4522..4541
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     LTR             4650..4883
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     misc_feature    4957..5545
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             5063..5074
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     5472..5492
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5552..5950)
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     CDS             complement(5951..6007)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(6017..6760)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     LTR             6788..7056
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     promoter        complement(7069..7280)
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     CDS             complement(7322..7348)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(8921..8947)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9024..9048)
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     protein_bind    9093..9111
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9129..9147
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9165..9183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9201..9219
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9237..9255
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9273..9291
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    9309..9327
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     misc_feature    9360..9476
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 attcttaaac ctaccaagcc tcctactatc attatgaata attttatata ccacagccaa
       61 tttgttatgt taaaccaatt ccacaaactt gcccatttat ctaattccaa taattcttgt
      121 tcattctttt cttgctggtt ttgcgattct tcaattaagg agtgtattaa gcttgtgtaa
      181 ttgttaattt ctctgtccca ctccatccag gtcgtgtgat tccaaatctg ttccagagat
      241 ttattactcc aactagcatt ccaaggcaca gcagtggtgc aaatgagttt tccagagcaa
      301 ccccaaatcc ccaggagctg ttgatccttt aggtatcttt ccacagccag gattcttgcc
      361 tggagctgct tgatgcccca gactgtgagt tgcaacagat gctgttgcgc ctcaatagcc
      421 ctcagcaaat tgttctgctg ctgcactata ccagacaata attgtctggc ctgtaccgtc
      481 agcgtcattg acgctgcgcc catagtgctt cctgctgctc ccaagaaccc aaggaacaaa
      541 gctcctattc ccactgctct tttttctctc tgcaccactc ttctctttgc cttggtgggt
      601 gctactccta atggttcaat ttttactact ttatatttat ataattcact tctccaattg
      661 tccctcatat ctcctcctcc aggtctgaag atcagtggcc gcttgctgtg cggtggtctt
      721 acttttgttt tgctcttcct ctatcttgtc taaagcttcc ttggtgtctt ttatctctat
      781 cctttgatgc acacaataga gggttgctac tgtattatat aatgatctaa gttcttctga
      841 tcctgtctga agggatggtt gtagctgtcc cagtatttgt ctacagcctt ctgatgtttc
      901 taacaggcca ggattaactg cgaatcgttc tagctccctg cttgcccata ctatatgttt
      961 taatttatat tttttctttc cccctggcct taaccgaatt ttttcccatc gcgatctaat
     1021 tctcccccgc ttaatactga cgctctcgca cccatctctc tccttctagc ctccgctagt
     1081 caaaattttt ggcgtactca ccagtcgccg cccctcgcct cttgccgtgc gcgcttcagc
     1141 aagccgagtc ctgcgtcgag agagctcctc tggtttccct ttcgctttca agtccctgtt
     1201 cgggcgccac tgctagagat tttccacact gactaaaagg gtctgaggga tctctagtta
     1261 ccagagtcac acaacagacg ggcacacact acttgaagca ctcaaggcaa gctttattga
     1321 ggcttaagca gtgggttccc tagttagcca gagagctccc aggctcagat ctggtctaac
     1381 cagagagacc cgtttatgta tcgagctagg cacttaaata caatatctct gcaatgcggc
     1441 aattcagtgg ttcgtccaat ccatgtcaga cccgtctgtt gccttcctaa taaggcacga
     1501 tcgtaccacc ttacttccac caatcggcat gcacggtgct ttttctctcc ttgtaaggca
     1561 tgttgctaac tcatcgttac catgttgcaa gactacaaga gtattgcata agactacacg
     1621 cgtcagcttg gcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     1681 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     1741 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg
     1801 gtattttctc cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac
     1861 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     1921 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     1981 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     2041 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     2101 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     2161 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     2221 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     2281 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     2341 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     2401 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     2461 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     2521 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     2581 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     2641 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     2701 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     2761 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     2821 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     2881 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     2941 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     3001 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     3061 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     3121 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     3181 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     3241 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     3301 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     3361 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     3421 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     3481 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     3541 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     3601 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     3661 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     3721 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     3781 atttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     3841 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     3901 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     3961 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     4021 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     4081 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     4141 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     4201 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgaca tgattacgaa
     4261 tttggtctct gcaaaagtct aggcctccaa aaaagcctcc tcactacttc tggaatagct
     4321 cagaggccga ggcggcctcg gcctctgcat aaataaaaaa aattagtcag ccatggggcg
     4381 gagaatgggc ggaactgggc ggagttaggg gcgggatggg cggagttagg ggcgggatag
     4441 ctagagccag acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag
     4501 tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata
     4561 agctgcaata aacaagttcc tctcactctc tgatattcat ttctttgcaa gttataaata
     4621 ctgaataata agatgacatg aactactact gctagagatt ttccacactg actaaaaggg
     4681 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
     4741 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
     4801 ggctcagatc tggtctaacc agagagaccc agtacaagca aaaagcagat cttatcttcg
     4861 ttgggagtga attagccctt ccagtccccc cttttctttt aaaaagtggc taagatctac
     4921 agctgccttg taagtcattg gtcttaaagg taccaggcgg ggaggcggcc caaagggaga
     4981 tccgactcgt ctgagggcga aggcgaagac gcggaagagg ccgcagagcc ggcagcaggc
     5041 cgcgggaagg aaggtccgct ggattgaggg ccgaagggac gtagcagaag gacgtcccgc
     5101 gcagaatcca ggtggcaaca caggcgagca gccaaggaaa ggacgatgat ttccccgaca
     5161 acaccacgga attgtcagtg cccaacagcc gagcccctgt ccagcagcgg gcaaggcagg
     5221 cggcgatgag ttccgccgtg gcaataggga gggggaaagc gaaagtcccg gaaaggagct
     5281 gacaggtggt ggcaatgccc caaccagtgg gggttgcgtc agcaaacaca gtgcacacca
     5341 cgccacgttg cctgacaacg ggccacaact cctcataaag agacagcaac caggatttat
     5401 acaaggagga gaaaatgaaa gccatacggg aagcaatagc atgatacaaa ggcattaaag
     5461 cagcgtatcc acatagcgta aaaggagcaa catagttaag aataccagtc aatctttcac
     5521 aaattttgta atccagaggt tgattgtcga cttagccctc ccacacataa ccagagggca
     5581 gcaattcacg aatcccaact gccgtcggct gtccatcact gtccttcact atggctttga
     5641 tcccaggatg cagatcgaga agcacctgtc ggcaccgtcc gcaggggctc aagatgcccc
     5701 tgttctcatt tccgatcgcg acgatacaag tcaggttgcc agctgccgca gcagcagcag
     5761 tgcccagcac cacgagttct gcacaaggtc ccccagtaaa atgatataca ttgacaccag
     5821 tgaagatgcg gccgtcgcta gagagagctg cgctggcgac gctgtagtct tcagagatgg
     5881 ggatgctgtt gattgtagcc gttgctcttt caatgagggt ggattcttct tgagacaaag
     5941 gcttggccat aggtccaggg ttctcctcca cgtctccagc ctgcttcagc aggctgaagt
     6001 tagtagctcc gcttccgcct ggcagcatat cgagatcgaa gtcatcgaga gcgtcagcag
     6061 ggagcatatc cagatcaaaa tcatccaggg catcggcggg cagcatgtcc aggtcgaagt
     6121 cgtccagggc gtcggtgggt ccgccgctct cgcacttcag ctgcttctcg aggccgcaga
     6181 tgatcagttc caggccgaac aggaaggcgg gctcggcgcc ctgtctgtcg aacagctcga
     6241 tggcctgctt cagcaggggg ggcatgctgt cggtggtggg tgtctctctt tcctctttgg
     6301 cgacctggtg ttcctgttct tccagcacgc agcccagggt gaagtggccc acggcgctca
     6361 gggcgtacag ggcgttttcc aggctgaagc cctgctggca caggaaggcc agctggtttt
     6421 ccagtgtctc gtactgcttc tcggtgggtc tggtgcccag gtgcactttg gcgccgtccc
     6481 ggtggctcag cagggcgcat ctgtagctct tggcgttgtt ccgcaggaag tcctgccagc
     6541 tctcgccttc cagagggcag ctgtgggtgt ggtgccggtc cagcatctcg atgggcaggg
     6601 cgtccagcag ggcccgcttg ttcttcacgt gccagtacag ggtgggctgt tccacgccca
     6661 gtttctgggc cagcttccgg gtggtcaggc cctcgatgcc cacgccgttc agcagttcca
     6721 gggcgctgtt gatcactttg ctcttgtcca gccggctcat ggtggcgtag gcgccggtca
     6781 cagcttggat ctgtaacggc gcagaacaga aaacgaaaca aagacgtaga gttgagcaag
     6841 cagggtcagg caaagcgtgg agagccggct gagtctaggt aggctccaag ggagcgccgg
     6901 acaaaggccc ggtctcgacc tgagctttaa acttacctag acggcggacg cagttcagga
     6961 ggcaccacag gcgggaggcg gcagaacgcg actcaaccgg cgtggatggc ggcctcaggt
     7021 agggcggcgg gcgcgtgaag gagagatgcg agcccctcga agcttcagct gtgttctggc
     7081 ggcaaacccg ttgcgaaaaa gaacgttcac ggcgactact gcacttatat acggttctcc
     7141 cccaccctcg ggaaaaaggc ggagccagta cacgacatca ctttcccagt ttaccccgcg
     7201 ccaccttctc taggcacccg ttcaattgcc gacccctccc cccaacttct cggggactgt
     7261 gggcgatgtg cgctctgccc actgacgggc accggagcga tcgcagatcc ttgcggcctt
     7321 aggcgtagtc tggcacgtcg taagggtagc tagctcaaga ccgtggcagg agcttgtatt
     7381 tgtacagcac aaaccccagg gcagttactg atgtggaaaa tccaatgatc ttcagcaggc
     7441 cagaaacaga gggcaggttc ctttcccaga aggcggccgc tgatgacagc ttgtttccat
     7501 gtattaaagg aatgacatat acagaaatgt cgtctcctga gcccagtcgg tcattagata
     7561 tccgccatcc tctgtccttc agcacacccc gggcacgcat caccaggtcc tgagctgcca
     7621 gtgtgtacct gtgaggatca tctggatcac agttaggaag aaactgagtg attgcttctg
     7681 ctacttcttc atttgataaa acgtcccaga gtccatcagt ggccaagatc agcacatcat
     7741 ctgatccatg atcatatttt gaaagatcgt agattcttac ctctggagct gaagacagga
     7801 atggtttaat gtagatgttg gagtcatgca ccttcaggtc atggtcccca agtcccctgg
     7861 tcactccaat agttgccatt acccgggcct tcttgccttc tccatatata agggggaact
     7921 tcaagtcctc atcctcaatg gttttgtatg cccagcctgt catattaaag tccctgtaga
     7981 gcatcttctt tccaagctcc tttctctgta ctctccttgg aaactccaaa tgtgtgaact
     8041 catttcccag caagtgaggc tgcatgaatg ccaggtactg aagtcgctgg cgctccgtct
     8101 cgggggtaaa ttctgaagac atggggataa tttctccatt tctgatgatt atggccctgc
     8161 tatccccagc atttgcaaca tacagcttcc ccaaaaggca aatcacaatg agggccgtgc
     8221 agccaccaga tatattatat gaactcctct ctcgttctat ctgtaggtcc atttccttga
     8281 atgcactttc aagcgctccg atgaccaggc actcatgggg aatcttcttc tcggtaaaga
     8341 agcgtgtggg gggcgtgctg ggggagcccg gggcccccac ccctccgcgc agggaggctg
     8401 cccgggtcag agtccggctg ttggcgggcg tgttctcagg ctcctccccc aggcaggtag
     8461 ggggcaggac ggcggagttc ttcaggatgt ccacgatgtc ctgcagctgc tccgtgatgt
     8521 ggtgctgcag caggcgtgac gccaccaccg cggccccgga ccccgcgtgc ccgtcaaaca
     8581 gcgaccaata gtggcaggaa acaccctcgg attccgagtt ctccttcagc tgcagccctt
     8641 ccccattggg aagggaggac cgtctcttgg atgagttcct gtttggggtt gaggtcacgg
     8701 cccctgcctt cttcttcaca gtgagcacct cacagctggc ttggtcttca ttgtgtgtgc
     8761 tcttcccggc attgataacc tctgcgtagc cagtggccca gggcagccgc cgagtctcct
     8821 tgaggatgag gatggggcgg gcgatgtggt cggcgctgca ctccacctcg tcctgagaca
     8881 gccccaggaa ctctggccgc ccgtagggga aacgggatcc ggcgtaatct ggcacatcgt
     8941 atgggtacat tctagattta cgagggtagg aagtggtacg gaaagttggt ataagacaaa
     9001 agtgttgtgg aattgctcca ggcgatctga cggttcacta aacgagctct gcttttatag
     9061 gcgcccaccg tacacgccta aagcttatac gttctctatc actgataggg agtaaactgg
     9121 atatacgttc tctatcactg atagggagta aactgtagat acgttctcta tcactgatag
     9181 ggagtaaact ggtcatacgt tctctatcac tgatagggag taaactcctt atacgttctc
     9241 tatcactgat agggagtaaa gtctgcatac gttctctatc actgataggg agtaaactct
     9301 tcatacgttc tctatcactg atagggagta aaccactgtg gggtggaact agtatcgata
     9361 aaattttgaa ttttgtaatt tgtttttgta attctttagt ttgtatgtct gttgctatta
     9421 tgtctactat tctttcccct gcactgtacc ccccaatccc cccttttctt ttaaaagtta
     9481 accgataccg tcgagatccg ttcactaatc gaatggatct gtctctgtct ctctctccac
     9541 cttcttcttc tattccttcg ggcctgtcgg gtcccctcgg ggttgggagg tgggtctgaa
     9601 acgataatgg tgaatatccc tgcctaactc tattcactat agaaagtaca gcaaaaact
//
