LOCUS       sequence-46902-e         649 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 649)
  AUTHORS   Colwill K, Wells CD, Elder K, Goudreault M, Hersi K, Kulkarni S, 
            Hardy WR, Pawson T, Morin GB
  TITLE     Modification of the Creator recombination system for proteomics 
            applications--improved expression by addition of splice sites.
  JOURNAL   BMC Biotechnol. 2006 Mar 6;6:13. doi: 10.1186/1472-6750-6-13.
  PUBMED    16519801
REFERENCE   2  (bases 1 to 649)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2006-03-6"; volume: "6:13. doi"; pages: " 
            10.1186/1472-6750-6-13"
COMMENT     Sequence Label: V678 pDNR MCS SA ES - Donor
FEATURES             Location/Qualifiers
     source          1..649
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      51..145
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     protein_bind    287..320
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(327..344)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(327..343)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(540..559)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tactgcgatg agtggcaggg cggggcgtaa tttgatatcg agctcgcttg gactcctgtt
       61 gatagatcca gtaatgacct cagaactcca tctggatttg ttcagaacgc tcggttgccg
      121 ccgggcgttt tttattggtg agaatccaag cactagtcat gaccaggatc tcctaggaac
      181 ttacctggtt aattaagtcg acggatcccg cggccgctga attcgcgccc ctggggaaaa
      241 aaaagggaca ggataagtat gatattgtca aggaaaccct cgcgccataa cttcgtataa
      301 tgtatgctat acgaagttat agatctactg gccgtcgttt tacacgcgta aggcggtaat
      361 acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca
      421 aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc
      481 tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata
      541 aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc
      601 gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgctt
//
