LOCUS       sequence-469047-       11140 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral plasmid expressing HA-tagged human progranulin followed 
            by IRES2-GFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11140)
  AUTHORS   Davis SE, Roth JR, Aljabi Q, Hakim AR, Savell KE, Day JJ, Arrant AE
  TITLE     Delivering progranulin to neuronal lysosomes protects against 
            excitotoxicity.
  JOURNAL   J Biol Chem. 2021 Sep;297(3):100993. doi: 10.1016/j.jbc.2021.100993.
            Epub 2021 Jul 21.
  PUBMED    34298019
REFERENCE   2  (bases 1 to 11140)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2021-09"; volume: "297(3):100993. doi"; pages: " 
            10.1016/j.jbc.2021.100993. Epub 2021 Jul 21"
COMMENT     Sequence Label: hSyn-HA-PGRN-IRES2-GFP
FEATURES             Location/Qualifiers
     source          1..11140
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(166..957,958..1026))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     789..808
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1027..1131)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(1206..1225)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        1397..1776
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        1778..1976
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1931..1951
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             1994..2174
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2221..2346
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    2839..3072
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3257..3301
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    3599..3716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3761..4208
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             4279..4305
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     misc_feature    join(6049..6623,6624..6626,6627..6635)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6216..6233)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     6443..6462
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(6636..6638,6639..6641,6642..7355)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(6636..6638,6639..6641,6642..7352)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6681..6702)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6942..6961)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7289..7310
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    7391..7979
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7444..7464)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7862..7873)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8051..8284
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(8310..8327)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8316..8540
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8586..9014
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8673..8692)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8883..8904
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9023..9043)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9028..9357
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9208..9343
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9270..9289
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9405..9452
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9471..9845
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9975..10096
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10012..10031)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10066..10085
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10145..10161)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10145..10161)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10158..10180)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10169..10185
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10193..10199,10200..10217,10218..10223))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10238..10259
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10376..10393)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10547..11135)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10627..10646)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
       61 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
      121 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
      181 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
      241 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
      301 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
      361 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
      421 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
      481 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
      541 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
      601 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
      661 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
      721 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
      781 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
      841 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
      901 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
      961 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     1021 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     1081 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     1141 ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct atggtgcact
     1201 ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc tgcttgtgtg
     1261 ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca aggcttgacc
     1321 gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg
     1381 gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc aattacgggg
     1441 tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt aaatggcccg
     1501 cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta tgttcccata
     1561 gtaacgccaa tagggacttt ccattgacgt caatgggtgg agtatttacg gtaaactgcc
     1621 cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga cgtcaatgac
     1681 ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt tcctacttgg
     1741 cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg gcagtacatc
     1801 aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc cattgacgtc
     1861 aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg taacaactcc
     1921 gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat aagcagcgcg
     1981 ttttgcctgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     2041 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     2101 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     2161 gaaaatctct agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag
     2221 ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga
     2281 ctggtgagta cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag
     2341 agcgtcagta ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca
     2401 gggggaaaga aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga
     2461 ttcgcagtta atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag
     2521 ctacaaccat cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca
     2581 accctctatt gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag
     2641 atagaggaag agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag
     2701 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     2761 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     2821 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     2881 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     2941 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     3001 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     3061 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     3121 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     3181 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
     3241 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
     3301 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
     3361 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
     3421 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
     3481 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
     3541 gaacggatcg gcactgcgtg cgccaattct gcagacaaat ggcagtattc atccacaatt
     3601 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     3661 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     3721 tttattacag ggacagcaga gatccagttt ggttaattaa agtgcaagtg ggttttagga
     3781 ccaggatgag gcggggtggg ggtgcctacc tgacgaccga ccccgaccca ctggacaagc
     3841 acccaacccc cattccccaa attgcgcatc ccctatcaga gagggggagg ggaaacagga
     3901 tgcggcgagg cgcgtgcgca ctgccagctt cagcaccgcg gacagtgcct tcgcccccgc
     3961 ctggcggcgc gcgccaccgc cgcctcagca ctgaaggcgc gctgacgtca ctcgccggtc
     4021 ccccgcaaac tccccttccc ggccaccttg gtcgcgtccg cgccgccgcc ggcccagccg
     4081 gaccgcacca cgcgaggcgc gagatagggg ggcacgggcg cgaccatctg cgctgcggcg
     4141 ccggcgactc agcgctgcct cagtctgcgg tgggcagcgg aggagtcgtg tcgtgcctga
     4201 gagcgcagac cggctcgagc caccatgtgg accctggtga gctgggtggc cttaacagca
     4261 gggctggtgg ctggaggtta cccatacgat gttccagatt acgctggtac gcggtgccca
     4321 gatggtcagt tctgccctgt ggcctgctgc ctggaccccg gaggagccag ctacagctgc
     4381 tgccgtcccc ttctggacaa atggcccaca acactgagca ggcatctggg tggcccctgc
     4441 caggttgatg cccactgctc tgccggccac tcctgcatct ttaccgtctc agggacttcc
     4501 agttgctgcc ccttcccaga ggccgtggca tgcggggatg gccatcactg ctgcccacgg
     4561 ggcttccact gcagtgcaga cgggcgatcc tgcttccaaa gatcaggtaa caactccgtg
     4621 ggtgccatcc agtgccctga tagtcagttc gaatgcccgg acttctccac gtgctgtgtt
     4681 atggtcgatg gctcctgggg gtgctgcccc atgccccagg cttcctgctg tgaagacagg
     4741 gtgcactgct gtccgcacgg tgccttctgc gacctggttc acacccgctg catcacaccc
     4801 acgggcaccc accccctggc aaagaagctc cctgcccaga ggactaacag ggcagtggcc
     4861 ttgtccagct cggtcatgtg tccggacgca cggtcccggt gccctgatgg ttctacctgc
     4921 tgtgagctgc ccagtgggaa gtatggctgc tgcccaatgc ccaacgccac ctgctgctcc
     4981 gatcacctgc actgctgccc ccaagacact gtgtgtgacc tgatccagag taagtgcctc
     5041 tccaaggaga acgctaccac ggacctcctc actaagctgc ctgcgcacac agtgggggat
     5101 gtgaaatgtg acatggaggt gagctgccca gatggctata cctgctgccg tctacagtcg
     5161 ggggcctggg gctgctgccc ttttacccag gctgtgtgct gtgaggacca catacactgc
     5221 tgtcccgcgg ggtttacgtg tgacacgcag aagggtacct gtgaacaggg gccccaccag
     5281 gtgccctgga tggagaaggc cccagctcac ctcagcctgc cagacccaca agccttgaag
     5341 agagatgtcc cctgtgataa tgtcagcagc tgtccctcct ccgatacctg ctgccaactc
     5401 acgtctgggg agtggggctg ctgtccaatc ccagaggctg tctgctgctc ggaccaccag
     5461 cactgctgcc cccagggcta cacgtgtgta gctgaggggc agtgtcagcg aggaagcgag
     5521 atcgtggctg gactggagaa gatgcctgcc cgccgggctt ccttatccca ccccagagac
     5581 atcggctgtg accagcacac cagctgcccg gtggggcaga cctgctgccc gagcctgggt
     5641 gggagctggg cctgctgcca gttgccccat gctgtgtgct gcgaggatcg ccagcactgc
     5701 tgcccggctg gctacacctg caacgtgaag gctcgatcct gcgagaagga agtggtctct
     5761 gcccagcctg ccaccttcct ggcccgtagc cctcacgtgg gtgtgaagga cgtggagtgt
     5821 ggggaaggac acttctgcca tgataaccag acctgctgcc gagacaaccg acagggctgg
     5881 gcctgctgtc cctaccgcca gggcgtctgt tgtgctgatc ggcgccactg ctgtcctgct
     5941 ggcttccgct gcgcagccag gggtaccaag tgtttgcgca gggaggcccc gcgctgggac
     6001 gcccctttga gggacccagc cttgagacag ctgctgtaat gagatccgcc cctctccctc
     6061 ccccccccct aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta
     6121 tatgttattt tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc
     6181 tgtcttcttg acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct
     6241 gttgaatgtc gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt
     6301 agcgaccctt tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa
     6361 gccacgtgta taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg
     6421 gatagttgtg gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga
     6481 tgcccagaag gtaccccatt gtatgggatc tgatctgggg cctcggtaca catgctttac
     6541 atgtgtttag tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt
     6601 cctttgaaaa acacgatgat aatatggcca caaccatggt gagcaagggc gaggagctgt
     6661 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     6721 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     6781 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
     6841 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
     6901 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     6961 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     7021 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     7081 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     7141 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca
     7201 tcggcgacgg ccccgtgctg ctgcccgaca accactacct gagcacccag tccgccctga
     7261 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     7321 ggatcactct cggcatggac gagctgtaca agtaatagaa ttcaattcta actagacgcg
     7381 ttaagtcgac aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     7441 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     7501 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     7561 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     7621 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     7681 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     7741 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     7801 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     7861 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     7921 tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg
     7981 tcgactttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8041 aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt
     8101 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8161 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8221 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8281 agcagggccc gtttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8341 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8401 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8461 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8521 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8581 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8641 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8701 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8761 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8821 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8881 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8941 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     9001 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     9061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     9121 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     9181 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     9241 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9301 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9361 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     9421 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     9481 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     9541 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     9601 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9661 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9721 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9781 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9841 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9901 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9961 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
    10021 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
    10081 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
    10141 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
    10201 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
    10261 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10321 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    10381 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10441 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10501 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10561 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10621 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10681 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10741 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10801 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10861 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10921 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10981 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    11041 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    11101 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
//
