LOCUS       sequence-469049-       14434 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Int13 Recombinase switch; plant T-DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14434)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 14434)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC210
FEATURES             Location/Qualifiers
     source          1..14434
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        542..887
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     830..850
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1085..1882
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1142..1161)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1752..1771
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1892..2140
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             2308..6258
                     /label=RUBY
     CDS             3808..3864
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4699..4755
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     misc_feature    6837..6861
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7286..8080
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9505..10093
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9994..10013
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10247..10264
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10279..10419
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10345..10364)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10505..10527
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10763..10957
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(11023..12096)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12525..13154)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttt
       61 tcgtacgacc ctcggtaccg atcggcgcgc cggagagatc gcgcacgcca atgctaccgt
      121 aggtcctgct tcaggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg
      181 tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt
      241 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      301 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
      361 atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      421 aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgataac atggtggagc
      481 acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta
      541 ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta
      601 tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt
      661 gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac
      721 ccccacccac gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag
      781 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      841 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tcgagtataa
      901 gagctcattt ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat
      961 ttacaattat cgatacaatc tgagaacacg acgctgagct cggacggcgc gccggagcgc
     1021 atacattgtt gttgtttttc cagatccagt tggtcctgta aatataagca atccatgtga
     1081 gtaaatgggg attgaacaag atggattgca cgcaggttct ccggccgctt gggtggagag
     1141 gctattcggc tatgactggg cacaacagac aatcggctgc tctgatgccg ccgtgtttcg
     1201 gctgtcagcg caggggcgcc cggttctttt tgtcaagacc gacctgtccg gtgccctgaa
     1261 tgaactccag gacgaggcag cgcggctatc gtggctggcc acgacgggcg ttccttgcgc
     1321 agctgtgctc gacgttgtca ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc
     1381 ggggcaggat ctcctgtcat ctcaccttgc tcctgccgag aaagtatcca tcatggctga
     1441 tgcaatgcgg cggctgcata cgcttgatcc ggctacctgc ccattcgacc accaagcgaa
     1501 acatcgcatc gagcgagcac gtactcggat ggaagccggt cttgtcgatc aggatgatct
     1561 ggacgaagaa catcaggggc tcgcgccagc cgaactgttc gccaggctca aggcgcgcat
     1621 gcccgacggc gaggatctcg tcgtgacaca tggcgatgcc tgcttgccga atatcatggt
     1681 ggaaaatggc cgcttttctg gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta
     1741 tcaggacata gcgttggcta cccgtgatat tgctgaagaa cttggcggcg aatgggctga
     1801 ccgcttcctc gtgctttacg gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg
     1861 ccttcttgac gagttcttct gagcgggctt atatgaagat gaagatgaaa tatttggtgt
     1921 gtcaaataaa aagcttgtgt gcttaagttt gtgttttttt cttggcttgt tgtgttatga
     1981 atttgtggct ttttctaata ttaaatgaat gtaagatcac attataatga ataaacaaat
     2041 gtttctataa tccattgtga atgttttgtt ggatctcttc tgcagcatat aactactgta
     2101 tgtgctatgg tatggactat ggaatatgat taaagataag cgctgagctc ccggggcgcg
     2161 ccggagagat cgcgcacgcc aatgctaccg taggtcctgc ttcaataacg gttgtatttg
     2221 tagaacttga ccagttgttt tagtaacata aatacaactc cgaatacgct gagctctccc
     2281 ggcgcgccgg agagatcgcg cacgccaatg gatcatgcga ccctcgccat gatcctcgcg
     2341 atctggttca tcagcttcca cttcatcaag ctgctgttct cccagcagac caccaagctg
     2401 cttccgccag gaccaaagcc gcttccgatc atcggcaaca tccttgaggt gggcaagaag
     2461 ccgcatcggt ccttcgccaa cctcgccaag attcacggcc cactcatttc cctcagactc
     2521 ggctctgtga ccaccatcgt tgtgtcctct gccgacgtgg ccaaagagat gttcctcaag
     2581 aaggatcacc cgctctccaa ccgcacgatc ccgaatagtg ttacagccgg cgaccaccac
     2641 aagctcacca tgtcttggct cccggtgtct ccgaagtggc gcaacttccg caagattacc
     2701 gccgtgcatc tgctctcccc acagagactc gatgcctgcc agacattcag gcacgccaag
     2761 gtgcagcagc tctacgagta cgttcaagag tgcgcccaga aaggccaggc cgtggatatt
     2821 ggcaaggccg cctttacgac cagcctcaac ctcctcagca agctgttctt cagcgtcgag
     2881 ctggcgcacc acaagtccca taccagccaa gagttcaaag agctgatctg gaacatcatg
     2941 gaagatatag gcaagccgaa ctacgccgac tacttcccga ttctcggctg cgttgaccca
     3001 tctggcatta gaagaaggct cgcctgctcc ttcgacaagc tgatcgccgt gttccagggc
     3061 atcatctgcg agagactcgc cccagattcc tccaccacaa ctaccaccac caccgacgac
     3121 gtgctcgatg tgctcctcca gctgttcaag cagaacgagc tgacgatggg cgagatcaac
     3181 cacctcctcg tggacatctt cgacgccggc accgatacca catcctccac attcgagtgg
     3241 gtgatgaccg agctgatccg caatccagag atgatggaaa aggcccaaga ggaaatcaag
     3301 caggtcctcg gcaaggacaa gcagatccaa gagtccgaca tcatcaacct gccgtacctc
     3361 caggcgatca tcaaagagac actccgcctc catccgccga ccgtgttctt gctcccaaga
     3421 aaggccgaca ccgatgtcga gctgtacggc tacatcgtgc cgaaggatgc ccagatcctc
     3481 gtgaacctct gggccattgg cagggaccca aacgcctggc agaacgccga tattttcagc
     3541 ccagagcgct tcatcggctg cgagatcgat gttaagggcc gcgatttcgg cctccttcca
     3601 tttggcgctg gccgcagaat ttgcccaggc atgaatctcg ccatcaggat gctcaccctc
     3661 atgctcgcca cactcctcca gttcttcaac tggaagctcg aaggcgacat ctccccgaag
     3721 gacctcgaca tggacgagaa gttcggcatt gcgctccaaa agaccaagcc gctcaagctc
     3781 atcccgattc cgcgctacgg tagcggagct accaatttta gcctccttaa gcaggctggt
     3841 gatgtagaag agaaccccgg gcctatgaag atgatgaacg gcgaggacgc caacgaccag
     3901 atgatcaaag agtccttctt catcacccac ggcaacccga tcctcaccgt cgaggataca
     3961 catccgctca ggccgttctt cgagacatgg cgcgagaaga ttttctccaa gaagccgaag
     4021 gccatcctca tcatctccgg ccactgggag acagtgaagc caaccgtgaa cgccgtgcac
     4081 atcaacgaca ccatccacga cttcgacgac tacccagccg ccatgtacca gttcaagtac
     4141 ccagctccag gcgagccaga gcttgcgaga aaggtggaag agatcctcaa gaagtccggg
     4201 ttcgagacag ccgagacaga ccaaaagagg ggccttgatc acggcgcctg ggttccactc
     4261 atgctcatgt atccagaggc ggacatcccg gtgtgccagc tctcagttca gccacatctc
     4321 gacggcacct accactacaa tctcggcaga gccctcgcgc cgctcaagaa tgatggcgtg
     4381 ctcattattg gctccggcag cgccacacat ccactcgatg agacaccgca ctacttcgat
     4441 ggtgttgccc cttgggccgc tgccttcgat tcttggctta ggaaggccct catcaacggc
     4501 cgcttcgagg aagtgaacat ctacgagagc aaggccccga actggaagct cgcccatcca
     4561 tttccagagc acttctaccc gctccacgtt gtgctcggcg ctgctggtga aaagtggaag
     4621 gccgagctga tccactcctc ctgggatcat ggcacacttt gccacggctc ctacaagttc
     4681 acctccgccg gatccggagc aaccaacttt agcctgctca agcaagcagg agatgttgag
     4741 gaaaatcctg gccccatgac cgccatcaag atgaacacca acggcgaggg cgagacacag
     4801 cacatcctca tgatcccgtt catggcgcag ggccacctca ggccatttct cgaactcgcc
     4861 atgttcctct acaagcgctc ccacgtgatc atcaccctgc tcacaactcc gctcaatgcc
     4921 ggcttcctca ggcacctcct tcaccaccat tcctactcct ccagcggcat caggatcgtc
     4981 gagctgccat tcaactccac caaccacgga ctcccaccgg gcatcgagaa caccgataag
     5041 ctcacactcc cgctcgtggt gtccctcttc cattccacca tcagcctcga tccgcacctc
     5101 cgcgattaca tctccaggca tttcagccca gccaggccac cactctgcgt gatccatgat
     5161 gtgttcctcg gctgggttga ccaggtggcc aaggatgtgg gctctacagg cgtggtgttc
     5221 acaacaggcg gcgcttatgg cacatccgcc tacgtgtcca tctggaacga tctcccgcac
     5281 cagaactact ccgacgacca agagttcccg ctgccaggct tcccagagaa ccataagttc
     5341 cgcaggtccc agctccatcg gttcctcaga tatgccgacg gctccgacga ttggtccaag
     5401 tatttccagc cgcagctccg ccagtccatg aagtcttttg gctggctctg caactccgtg
     5461 gaagagatcg agacactcgg cttctccatc ctccgcaact acaccaagct gccgatctgg
     5521 ggcatcggcc cacttattgc ttccccagtg cagcactcct cctccgacaa caattcaaca
     5581 ggcgccgagt tcgtgcagtg gctcagcctc aaagagccgg actccgtcct ctacatctcc
     5641 ttcggctccc agaacacgat cagcccgacg cagatgatgg aactcgctgc tggccttgag
     5701 tcctccgaga agccattcct ctgggtgatc agagccccgt tcggcttcga catcaacgaa
     5761 gagatgcgcc cagagtggct gccagagggc tttgaggaac gcatgaaggt gaagaaacag
     5821 ggcaagctcg tgtacaagct cggcccgcag cttgagatcc tcaaccatga atccatcggc
     5881 ggctttctca cccactgcgg atggaacagc atccttgagt ctcttcgcga gggcgttccg
     5941 atgcttggat ggccacttgc tgccgagcag gcctacaacc tcaagtacct cgaagatgag
     6001 atgggcgtcg cggttgagct tgctagaggc ctcgaaggcg agatctccaa agagaaggtc
     6061 aagcgcatcg tcgagatgat ccttgagcgc aacgagggct ccaaaggctg ggagatgaag
     6121 aatcgcgccg tggaaatggg caaaaagctc aaggacgccg tgaacgagga aaaagagctg
     6181 aagggctcct ccgtgaaggc gatcgacgat ttcctcgacg ccgtcatgca ggccaaactt
     6241 gagccaagcc tccagtgata gtgagcttac tgaggaaata tatagacaaa ttaagtttgg
     6301 ttctatgagt tctaatttgg acttaagagt tgtttgaaat tctattttat agtgatgctt
     6361 ataatgtatt tggactgttt tctgctgtgt gtaagacctt ttggtctgtg aactggaaac
     6421 atacatgaat aaatttcttt gaatttactg gaatttttgc atcaacaaaa gaaaaattga
     6481 agttactaac ttgtaaatgg aacaattgta atgttaaagg atataaatat cttaatatag
     6541 tgcgatacga atcacacgaa tgcaagactt tctctctctg ctcccgctca tgctctcggt
     6601 gcatgttagc taaatataca tcggtgcatc catggcagga gcatgaggac ggggatgagg
     6661 aagggagtga ggagggccaa aagaagtaca catagtttcc tttgggagcg aattccgctg
     6721 agctcagtgt ttgatcgccg gcggtaccga gtgtacttca agtcactcaa ttcggcgtta
     6781 attcagtaca ttaaaaacgt ccgcaatgtg ttattaagtt gtctaagcgt caatttgttt
     6841 acaccacaat atatcctgcc accagccagc caacagctcc ccgaccggca gctcggcaca
     6901 aaatcaccac tcgatacagg cagcccatca gtccgggacg gcgtcagcgg gagagccgtt
     6961 gtaaggcggc agactttgct catgttaccg atgctattcg gaagaacggc aactaagctg
     7021 ccgggtttga aacacggatg atctcgcgga gggtagcatg ttgattgtaa cgatgacaga
     7081 gcgttgctgc ctgtgatcac cgcggtttca aaatcggctc cgtcgatact atgttatacg
     7141 ccaactttga aaacaacttt gaaaaagctg ttttctggta tttaaggttt tagaatgcaa
     7201 ggaacagtga attggagttc gtcttgttat aattagcttc ttggggtatc tttaaatact
     7261 gtagaaaaga ggaaggaaat aataaatggc taaaatgaga atatcaccgg aattgaaaaa
     7321 actgatcgaa aaataccgct gcgtaaaaga tacggaagga atgtctcctg ctaaggtata
     7381 taagctggtg ggagaaaatg aaaacctata tttaaaaatg acggacagcc ggtataaagg
     7441 gaccacctat gatgtggaac gggaaaagga catgatgcta tggctggaag gaaagctgcc
     7501 tgttccaaag gtcctgcact ttgaacggca tgatggctgg agcaatctgc tcatgagtga
     7561 ggccgatggc gtcctttgct cggaagagta tgaagatgaa caaagccctg aaaagattat
     7621 cgagctgtat gcggagtgca tcaggctctt tcactccatc gacatatcgg attgtcccta
     7681 tacgaatagc ttagacagcc gcttagccga attggattac ttactgaata acgatctggc
     7741 cgatgtggat tgcgaaaact gggaagaaga cactccattt aaagatccgc gcgagctgta
     7801 tgatttttta aagacggaaa agcccgaaga ggaacttgtc ttttcccacg gcgacctggg
     7861 agacagcaac atctttgtga aagatggcaa agtaagtggc tttattgatc ttgggagaag
     7921 cggcagggcg gacaagtggt atgacattgc cttctgcgtc cggtcgatca gggaggatat
     7981 cggggaagaa cagtatgtcg agctattttt tgacttactg gggatcaagc ctgattggga
     8041 gaaaataaaa tattatattt tactggatga attgttttag tacctagaat gcatgaccaa
     8101 aatcccttaa cgctgatgaa tcccctaatg attttggtaa aaatcattaa gttaaggtgg
     8161 atacacatct tgtcatatga tcaaatggtt tcgcgaaaaa tcaataatca gacaacaaga
     8221 tgtgcgaact cgatatttta cacgactctc tttaccaatt ctgccccgaa ttacacttaa
     8281 aacgactcaa cagcttaacg ttggcttgcc acgcattact tgactgtaaa actctcactc
     8341 ttaccgaact tggccgtaac ctgccaacca aagcgagaac aaaacataac atcaaacgaa
     8401 tcgaccgatt gttaggtaat cgtcacctcc acaaagagcg actcgctgta taccgttggc
     8461 atgctagctt tatctgttcg ggcaatacga tgcccattgt acttgttgac tggtctgata
     8521 ttcgtgagca aaaacgactt atggtattgc gagcttcagt cgcactacac ggtcgttctg
     8581 ttactcttta tgagaaagcg ttcccgcttt cagagcaatg ttcaaagaaa gctcatgacc
     8641 aatttctagc cgaccttgcg agcattctac cgagtaacac cacaccgctc attgtcagtg
     8701 atgctggctt taaagtgcca tggtataaat ccgttgagaa gctgggttgg tactggttaa
     8761 gtcgagtaag aggaaaagta caatatgcag acctaggagc ggaaaactgg aaacctatca
     8821 gcaacttaca tgatatgtca tctagtcact caaagacttt aggctataag aggctgacta
     8881 aaagcaatcc aatctcatgc caaattctat tgtataaatc tcgctctaaa ggccgaaaaa
     8941 atcagcgctc gacacggact cattgtcacc acccgtcacc taaaatctac tcagcgtcgg
     9001 caaaggagcc atgggttcta gcaactaact tacctgttga aattcgaaca cccaaacaac
     9061 ttgttaatat ctattcgaag cgaatgcaga ttgaagaaac cttccgagac ttgaaaagtc
     9121 ctgcctacgg actaggccta cgccatagcc gaacgagcag ctcagagcgt tttgatatca
     9181 tgctgctaat cgccctgatg cttcaactaa catgttggct tgcgggcgtt catgctcaga
     9241 aacaaggttg ggacaagcac ttccaggcta acacagtcag aaatcgaaac gtactctcaa
     9301 cagttcgctt aggcatggaa gttttgcggc attctggcta cacaataaca agggaagact
     9361 tactcgtggc tgcaacccta ctagctcaaa atttattcac acatggttac gctttgggga
     9421 aattatgagg ggatctctca gcccttaacg tgagttttcg ttccactgag cgtcagaccc
     9481 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     9541 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     9601 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     9661 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     9721 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     9781 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     9841 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     9901 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     9961 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
    10021 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
    10081 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
    10141 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
    10201 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
    10261 cgaggaagcg gaagagggcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc
    10321 acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt taagccagta
    10381 tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc gccaacaccc
    10441 gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca agctgtgacc
    10501 gtttccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag
    10561 ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc cgcgccctgg
    10621 tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga taggccgacg
    10681 cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt gcagctcttg
    10741 ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag ttttaataag
    10801 ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag tcacttacat
    10861 gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc ggttcccaat
    10921 gtacggcttt gggttcccaa tgtacgtgct atccacagga aagaggcctt ttcgaccttt
    10981 ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac cggcggatgc
    11041 ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct gccccgcctc
    11101 ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca cggtgaaaca
    11161 gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga acaaccatct
    11221 ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc cgatgccggg
    11281 atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc cttctgtgat
    11341 ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc cggtttcgct
    11401 ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca ccaggcggcc
    11461 gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc gaatgcaggt
    11521 ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact tgagtacgtc
    11581 cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc ggtatcggtt
    11641 catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc acacactggc
    11701 catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt agcggatcac
    11761 ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca tgatgctgcg
    11821 actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt gctcgtcgcc
    11881 cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg attctttgcg
    11941 gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct cgatgcgttg
    12001 ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca gcgccgcgcc
    12061 gatttgtacc gggccggatg gtttgcgacc gctcacgccg attcctcggg cttgggggtt
    12121 ccagtgccat tgcagggccg gcaggcaacc cagccgctta cgcctggcca accgcccgtt
    12181 cctccacaca tggggcattc cacggcgtcg gtgcctggtt gttcttgatt ttccatgccg
    12241 cctcctttag ccgctaaaat tcatctactc atttattcat ttgctcattt actctggtag
    12301 ctgcgcgatg tattcagata gcagctcggt aatggtcttg ccttggcgta ccgcgtacat
    12361 cttcagcttg gtgtgatcct ccgccggcaa ctgaaagttg acccgcttca tggctggcgt
    12421 gtctgccagg ctggccaacg ttgcagcctt gctgctgcgt gcgctcggac ggccggcact
    12481 tagcgtgttt gtgcttttgc tcattttctc tttacctcat taactcaaat gagttttgat
    12541 ttaatttcag cggccagcgc ctggacctcg cgggcagcgt cgccctcggg ttctgattca
    12601 agaacggttg tgccggcggc ggcagtgcct gggtagctca cgcgctgcgt gatacgggac
    12661 tcaagaatgg gcagctcgta cccggccagc gcctcggcaa cctcaccgcc gatgcgcgtg
    12721 cctttgatcg cccgcgacac gacaaaggcc gcttgtagcc ttccatccgt gacctcaatg
    12781 cgctgcttaa ccagctccac caggtcggcg gtggcccata tgtcgtaagg gcttggctgc
    12841 accggaatca gcacgaagtc ggctgccttg atcgcggaca cagccaagtc cgccgcctgg
    12901 ggcgctccgt cgatcactac gaagtcgcgc cggccgatgg ccttcacgtc gcggtcaatc
    12961 gtcgggcggt cgatgccgac aacggttagc ggttgatctt cccgcacggc cgcccaatcg
    13021 cgggcactgc cctggggatc ggaatcgact aacagaacat cggccccggc gagttgcagg
    13081 gcgcgggcta gatgggttgc gatggtcgtc ttgcctgacc cgcctttctg gttaagtaca
    13141 gcgataacct tcatgcgttc cccttgcgta tttgtttatt tactcatcgc atcatatacg
    13201 cagcgaccgc atgacgcaag ctgttttact caaatacaca tcaccttttt agacggcggc
    13261 gctcggtttc ttcagcggcc aagctggccg gccaggccgc cagcttggca tcagacaaac
    13321 cggccaggat ttcatgcagc cgcacggttg agccgtgcgc gggcggctcg aacacgtacc
    13381 cggccgcgat catctccgcc tcgatctctt cggtaatgaa aaacggttcg tcctggccgt
    13441 cctggtgcgg tttcatgctt gttcctcttg gcgttcattc tcggcggccg ccagggcgtc
    13501 ggcctcggtc aatgcgtcct cacggaaggc accgcgccgc ctggcctcgg tgggcgtcac
    13561 ttcctcgctg cgctcaagtg cgcggtacag ggtcgagcga tgcacgccaa gcagtgcagc
    13621 cgcctctttc acggtgcggc cttcctggtc gatcagctcg cgggcgtgcg cgatctgtgc
    13681 cggggtgagg gtagggcggg ggccaaactt cacgcctcgg gccttggcgg cctcgcgccc
    13741 gctccgggtg cggtcgatga ttagggaacg ctcgaactcg gcaatgccgg cgaacacggt
    13801 caacaccatg cggccggccg gcgtggtggt gtcggcccac ggctctgcca ggctacgcag
    13861 gcccgcgccg gcctcctgga tgcgctcggc aatgtccagt aggtcgcggg tgctgcgggc
    13921 caggcggtct agcctggtca ctgtcacaac gtcgccaggg cgtaggtggt caagcatcct
    13981 ggccagctcc gggcggtcgc gcctggtgcc ggtgatcttc tcggaaaaca gcttggtgca
    14041 gccggccgcg tgcagttcgg cccgttggtt ggtcaagtcc tggtcgtcgg tgctgacgcg
    14101 ggcatagccc agcaggccag cggcggcgct cttgttcatg gcgtaatgtc tccggttcta
    14161 gtcgcaagta ttctacttta tgcgactaaa acacgcgaca agaaaacgcc aggaaaaggg
    14221 cagggcggca gcctgtcgcg taacttagga cttgtgcgac atgtcgtttt cagaagacgg
    14281 ctgcactgaa cgtcagaagc cgactgcact atagcagcgg aggggttgga tcaaagtact
    14341 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
    14401 ttttcacgcc cttttaaata tccgattatt ctaa
//
