LOCUS       sequence-469051-       11142 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRV2 expressing CinH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 11142)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC062
FEATURES             Location/Qualifiers
     source          1..11142
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        554..899
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     842..862
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3001..3021
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             3490..3507
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3542..3794
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    3816..3840
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5141..5770
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6199..7272
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7338..7532
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(7768..7790)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    7876..8016
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     7931..7950
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8031..8048)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8202..8790)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8282..8301)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(9924..10718)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      181 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      241 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      301 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      361 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      421 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      481 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      541 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      601 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      661 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      721 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
      901 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
      961 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1021 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1081 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1141 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1201 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1261 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1321 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1381 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1441 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1501 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1561 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1621 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1681 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1741 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1801 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1861 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     1921 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     1981 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2041 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2101 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2161 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2221 gtagcatttg agtttcggag catcttgttc tggggtttca cactatcttt agagaaagtg
     2281 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2341 agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact
     2401 gtatcactta cccgagttaa cgagtctaga aacatgggga agggacaaaa ggttggctac
     2461 gttcgtgttt catcggttga gcaaaacact gggcggcagc tcgagggcat cgaggtggat
     2521 aggatcttcg tggacagggc ttccggcaag aataccgata gaccgaagtt ccaggaaatg
     2581 ctcaactacg tcagggaagg cgaccgcgtg atcgtccact caatggatag gttcgctagg
     2641 tccctcaagg acttggttac cgaggtcgac aaattggtca agagggggat tgccattcaa
     2701 ttcgtcaagg agaacatcac tttcaccgca cagtccactc caatggacaa cctcatgctg
     2761 cagcttatgg gcgccttcgc gcaatttgag agagagatta tacttgagcg gcagaaggag
     2821 ggtattaaga tcgctgccgc ccaaggaaag tacaaaggta gggtgcataa actcaacccg
     2881 gaccaggcta aggctttgct acaagcgtgg aaggagggca agtattctag caaggttgac
     2941 ctcgccaaag cgttcggcat atccaggcaa gccgtctaca gatacctcaa gcagatcaat
     3001 cctaagaaaa agcggaaagt gtgaagtgcc gggcatgtcc cgaagacatt aaactacggt
     3061 tctttaagta gatccgtgtc tgaagtttta ggttcaattt aaacctacga gattgacatt
     3121 ctcgactgat cttgattgat cggtaagtct tttgtaattt aattttcttt ttgattttat
     3181 tttaaattgt tatctgtttc tgtgtataga ctgtttgaga tcggcgtttg gccgactcat
     3241 tgtcttacca taggggaacg gactttgttt gtgttgttat tttatttgta ttttattaaa
     3301 attctcaacg atctgaaaaa gcctcgcggc taagagattg ttggggggtg agtaagtact
     3361 tttaaagtga tgatggttac aaaggcaaaa ggggtaaaac ccctcgccta cgtaagcgtt
     3421 attacgcccg tctgtactta tatcagtaca ctgacgagtc cctaaaggac gaaacgggag
     3481 aacgctagcc accaccacca ccaccacgtg tgaattacag gtgaccagct cgaatttccc
     3541 cgatcgttca aacatttggc aataaagttt cttaagattg aatcctgttg ccggtcttgc
     3601 gatgattatc atataatttc tgttgaatta cgttaagcat gtaataatta acatgtaatg
     3661 catgacgtta tttatgagat gggtttttat gattagagtc ccgcaattat acatttaata
     3721 cgcgatagaa aacaaaatat agcgcgcaaa ctaggataaa ttatcgcgcg cggtgtcatc
     3781 tatgttacta gatcgggaat taaactatca gtgtttgaca ggatatattg gcgggtaaac
     3841 ctaagagaaa agagcgttta ttagaataat cggatattta aaagggcgtg aaaaggttta
     3901 tccgttcgtc catttgtatg tgcatgccaa ccacagggtt cccctcggga tcaaagtact
     3961 ttgatccaac ccctccgctg ctatagtgca gtcggcttct gacgttcagt gcagccgtct
     4021 tctgaaaacg acatgtcgca caagtcctaa gttacgcgac aggctgccgc cctgcccttt
     4081 tcctggcgtt ttcttgtcgc gtgttttagt cgcataaagt agaatacttg cgactagaac
     4141 cggagacatt acgccatgaa caagagcgcc gccgctggcc tgctgggcta tgcccgcgtc
     4201 agcaccgacg accaggactt gaccaaccaa cgggccgaac tgcacgcggc cggctgcacc
     4261 aagctgtttt ccgagaagat caccggcacc aggcgcgacc gcccggagct ggccaggatg
     4321 cttgaccacc tacgccctgg cgacgttgtg acagtgacca ggctagaccg cctggcccgc
     4381 agcacccgcg acctactgga cattgccgag cgcatccagg aggccggcgc gggcctgcgt
     4441 agcctggcag agccgtgggc cgacaccacc acgccggccg gccgcatggt gttgaccgtg
     4501 ttcgccggca ttgccgagtt cgagcgttcc ctaatcatcg accgcacccg gagcgggcgc
     4561 gaggccgcca aggcccgagg cgtgaagttt ggcccccgcc ctaccctcac cccggcacag
     4621 atcgcgcacg cccgcgagct gatcgaccag gaaggccgca ccgtgaaaga ggcggctgca
     4681 ctgcttggcg tgcatcgctc gaccctgtac cgcgcacttg agcgcagcga ggaagtgacg
     4741 cccaccgagg ccaggcggcg cggtgccttc cgtgaggacg cattgaccga ggccgacgcc
     4801 ctggcggccg ccgagaatga acgccaagag gaacaagcat gaaaccgcac caggacggcc
     4861 aggacgaacc gtttttcatt accgaagaga tcgaggcgga gatgatcgcg gccgggtacg
     4921 tgttcgagcc gcccgcgcac gtctcaaccg tgcggctgca tgaaatcctg gccggtttgt
     4981 ctgatgccaa gctggcggcc tggccggcca gcttggccgc tgaagaaacc gagcgccgcc
     5041 gtctaaaaag gtgatgtgta tttgagtaaa acagcttgcg tcatgcggtc gctgcgtata
     5101 tgatgcgatg agtaaataaa caaatacgca aggggaacgc atgaaggtta tcgctgtact
     5161 taaccagaaa ggcgggtcag gcaagacgac catcgcaacc catctagccc gcgccctgca
     5221 actcgccggg gccgatgttc tgttagtcga ttccgatccc cagggcagtg cccgcgattg
     5281 ggcggccgtg cgggaagatc aaccgctaac cgttgtcggc atcgaccgcc cgacgattga
     5341 ccgcgacgtg aaggccatcg gccggcgcga cttcgtagtg atcgacggag cgccccaggc
     5401 ggcggacttg gctgtgtccg cgatcaaggc agccgacttc gtgctgattc cggtgcagcc
     5461 aagcccttac gacatatggg ccaccgccga cctggtggag ctggttaagc agcgcattga
     5521 ggtcacggat ggaaggctac aagcggcctt tgtcgtgtcg cgggcgatca aaggcacgcg
     5581 catcggcggt gaggttgccg aggcgctggc cgggtacgag ctgcccattc ttgagtcccg
     5641 tatcacgcag cgcgtgagct acccaggcac tgccgccgcc ggcacaaccg ttcttgaatc
     5701 agaacccgag ggcgacgctg cccgcgaggt ccaggcgctg gccgctgaaa ttaaatcaaa
     5761 actcatttga gttaatgagg taaagagaaa atgagcaaaa gcacaaacac gctaagtgcc
     5821 ggccgtccga gcgcacgcag cagcaaggct gcaacgttgg ccagcctggc agacacgcca
     5881 gccatgaagc gggtcaactt tcagttgccg gcggaggatc acaccaagct gaagatgtac
     5941 gcggtacgcc aaggcaagac cattaccgag ctgctatctg aatacatcgc gcagctacca
     6001 gagtaaatga gcaaatgaat aaatgagtag atgaatttta gcggctaaag gaggcggcat
     6061 ggaaaatcaa gaacaaccag gcaccgacgc cgtggaatgc cccatgtgtg gaggaacggg
     6121 cggttggcca ggcgtaagcg gctgggttgc ctgccggccc tgcaatggca ctggaacccc
     6181 caagcccgag gaatcggcgt gagcggtcgc aaaccatccg gcccggtaca aatcggcgcg
     6241 gcgctgggtg atgacctggt ggagaagttg aaggccgcgc aggccgccca gcggcaacgc
     6301 atcgaggcag aagcacgccc cggtgaatcg tggcaagcgg ccgctgatcg aatccgcaaa
     6361 gaatcccggc aaccgccggc agccggtgcg ccgtcgatta ggaagccgcc caagggcgac
     6421 gagcaaccag attttttcgt tccgatgctc tatgacgtgg gcacccgcga tagtcgcagc
     6481 atcatggacg tggccgtttt ccgtctgtcg aagcgtgacc gacgagctgg cgaggtgatc
     6541 cgctacgagc ttccagacgg gcacgtagag gtttccgcag ggccggccgg catggccagt
     6601 gtgtgggatt acgacctggt actgatggcg gtttcccatc taaccgaatc catgaaccga
     6661 taccgggaag ggaagggaga caagcccggc cgcgtgttcc gtccacacgt tgcggacgta
     6721 ctcaagttct gccggcgagc cgatggcgga aagcagaaag acgacctggt agaaacctgc
     6781 attcggttaa acaccacgca cgttgccatg cagcgtacga agaaggccaa gaacggccgc
     6841 ctggtgacgg tatccgaggg tgaagccttg attagccgct acaagatcgt aaagagcgaa
     6901 accgggcggc cggagtacat cgagatcgag ctagctgatt ggatgtaccg cgagatcaca
     6961 gaaggcaaga acccggacgt gctgacggtt caccccgatt actttttgat cgatcccggc
     7021 atcggccgtt ttctctaccg cctggcacgc cgcgccgcag gcaaggcaga agccagatgg
     7081 ttgttcaaga cgatctacga acgcagtggc agcgccggag agttcaagaa gttctgtttc
     7141 accgtgcgca agctgatcgg gtcaaatgac ctgccggagt acgatttgaa ggaggaggcg
     7201 gggcaggctg gcccgatcct agtcatgcgc taccgcaacc tgatcgaggg cgaagcatcc
     7261 gccggttcct aatgtacgga gcagatgcta gggcaaattg ccctagcagg ggaaaaaggt
     7321 cgaaaaggtc tctttcctgt ggatagcacg tacattggga acccaaagcc gtacattggg
     7381 aaccggaacc cgtacattgg gaacccaaag ccgtacattg ggaaccggtc acacatgtaa
     7441 gtgactgata taaaagagaa aaaaggcgat ttttccgcct aaaactcttt aaaacttatt
     7501 aaaactctta aaacccgcct ggcctgtgca taactgtctg gccagcgcac agccgaagag
     7561 ctgcaaaaag cgcctaccct tcggtcgctg cgctccctac gccccgccgc ttcgcgtcgg
     7621 cctatcgcgg ccgctggccg ctcaaaaatg gctggcctac ggccaggcaa tctaccaggg
     7681 cgcggacaag ccgcgccgtc gccactcgac cgccggcgcc cacatcaagg caccctgcct
     7741 cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg agacggtcac
     7801 agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt
     7861 tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag tgtatactgg
     7921 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
     7981 ccgcacagat gcgtaaggag aaaataccgc atcaggcgct cttccgcttc ctcgctcact
     8041 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     8101 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     8161 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     8221 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     8281 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     8341 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     8401 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     8461 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     8521 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     8581 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     8641 aggacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     8701 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     8761 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     8821 gacgctcagt ggaacgaaaa ctcacgttaa gggctgatga atcccctaat gatttttatc
     8881 aaaatcatta agttaaggta gatacacatc ttgtcatatg atcaaatggt ttcgccaaaa
     8941 atcaataatc agacaacaaa atgtgcgaac tcgatatttt acacgactct ctttaccaat
     9001 tctgccccga attacactta aaacgactca acagcttaac gttggcttgc cacgccttac
     9061 ttgactgtaa aactctcact cttaccgaac ttggccgtaa cctgccaacc aaagcgagaa
     9121 caaaacataa catcaaacga atcgaccgat tgttaggtaa tcgtcacctc cacaaagagc
     9181 gactcgctgt ataccgttgg catgctagct ttatctgttc gggcaatacg atgcccattg
     9241 tacttgttga ctggtctgat atccgtgagc aaaaacggct tatggtattg cgagcttcag
     9301 tcgcactaca cggtcgttct gttactcttt atgagaaagc gttcccgctt tcagagcaat
     9361 gttcaaagaa agctcatgac caatttctag ccgaccttgc gagcattcta ccgagtaaca
     9421 ccacaccgct cattgtcagt gatgctggct ttaaagtgcc atggattcta gcaactaact
     9481 tacctgttga aattcgaaca cccaaacaac ttgttaatat ctattcgaag cgaatgcaga
     9541 ttgaagaaac cttccgagac ttgaaaagtc ctgcctacgg actaggccta cgccatagcc
     9601 gaacgagcag ctcagagcgt tttgatatca tgctgctaat cgccctgatg cttcaactaa
     9661 catgttggct tgcgggcgtt catgctcaga aacaaggttg ggacaagcac ttccaggcta
     9721 acacagtcag aaatcgaaac gtactctcaa cagttcgctt aggcatggaa gttttgcggc
     9781 attctggcta cacaataaca agggaagact cactcgtggc tgcaaccctg cttactcaaa
     9841 atctattcac acatggttac gttttgggga aattatgagg ggatctctca gcgttaaggg
     9901 attttggtca tgcattctag gtactaaaac aattcatcca gtaaaatata atattttatt
     9961 ttctcccaat caggcttgat ccccagtaag tcaaaaaata gctcgacata ctgttcttcc
    10021 ccgatatcct ccctgatcga ccggacgcag aaggcaatgt cataccactt gtccgccctg
    10081 ccgcttctcc caagatcaat aaagccactt actttgccat ctttcacaaa gatgttgctg
    10141 tctcccaggt cgccgtggga aaagacaagt tcctcttcgg gcttttccgt ctttaaaaaa
    10201 tcatacagct cgcgcggatc tttaaatgga gtgtcttctt cccagttttc gcaatccaca
    10261 tcggccagat cgttattcag taagtaatcc aattcggcta agcggctgtc taagctattc
    10321 gtatagggac aatccgatat gtcgatggag tgaaagagcc tgatgcactc cgcatacagc
    10381 tcgataatct tttcagggct ttgttcatct tcatactctt ccgagcaaag gacgccatcg
    10441 gcctcactca tgagcagatt gctccagcca tcatgccgtt caaagtgcag gacctttgga
    10501 acaggcagct ttccttccag ccatagcatc atgtcctttt cccgttccac atcataggtg
    10561 gtccctttat accggctgtc cgtcattttt aaatataggt tttcattttc tcccaccagc
    10621 ttatatacct tagcaggaga cattccttcc gtatctttta cgcagcggta tttttcgatc
    10681 agttttttca attccggtga tattctcatt ttagccattt attatttcct tcctcttttc
    10741 tacagtattt aaagataccc caagaagcta attataacaa gacgaactcc aattcactgt
    10801 tccttgcatt ctaaaacctt aaataccaga aaacagcttt ttcaaagttg ttttcaaagt
    10861 tggcgtataa catagtatcg acggagccga ttttgaaacc gcggtgatca caggcagcaa
    10921 cgctctgtca tcgttacaat caacatgcta ccctccgcga gatcatccgt gtttcaaacc
    10981 cggcagctta gttgccgttc ttccgaatag catcggtaac atgagcaaag tctgccgcct
    11041 tacaacggct ctcccgctga cgccgtcccg gactgatggg ctgcctgtat cgagtggtga
    11101 ttttgtgccg agctgccggt cggggagctg ttggctggct gg
//
