LOCUS       sequence-46907-e         699 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mycobacteria replicating vector expressing eGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 699)
  AUTHORS   Seeliger JC, Topp S, Sogi KM, Previti ML, Gallivan JP, Bertozzi CR
  TITLE     A riboswitch-based inducible gene expression system for 
            mycobacteria.
  JOURNAL   PLoS One. 2012;7(1):e29266. Epub 2012 Jan 18.
  PUBMED    22279533
REFERENCE   2  (bases 1 to 699)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2012"; volume: "7(1)"; pages: "e29266. Epub 2012 Jan 18"
COMMENT     Sequence Label: pST5552
FEATURES             Location/Qualifiers
     source          1..699
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      69..115
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(265..284)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     primer_bind     complement(349..368)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
ORIGIN
        1 cgagctgtac aagtaaaagc ttatcgatgt cgacgtagtt aactagcgta cgatcgactg
       61 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatgccat
      121 catggccgcg gtgatcagct agccacctga cgtcgggggg gggggaaagc cacgttgtgt
      181 ctcaaaatct ctgatgttac attgcacaag ataaaaatat atcatcatga acaataaaac
      241 tgtctgctta cataaacagt aatacaaggg gtgttatgag ccatattcaa cgggaaacgt
      301 cttgctcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
      361 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagccccatg
      421 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
      481 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
      541 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cgggaaaaca gcattccagg
      601 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgttcctgc
      661 gccggttgca ttcgattcct gtttgtaatt gtcctttta
//
