LOCUS       sequence-46910-e       10814 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10814)
  AUTHORS   Roberts JN, Buck CB, Thompson CD, Kines R, Bernardo M, Choyke PL, 
            Lowy DR, Schiller JT
  TITLE     Genital transmission of HPV in a mouse model is potentiated by 
            nonoxynol-9 and inhibited by carrageenan.
  JOURNAL   Nat Med. 2007 Jul;13(7):857-61. Epub 2007 Jul 1.
  PUBMED    17603495
REFERENCE   2  (bases 1 to 10814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Med."; 
            date: "2007-07"; volume: "13(7)"; pages: "857-61. Epub 2007 Jul 1"
COMMENT     Sequence Label: p45sheLL
FEATURES             Location/Qualifiers
     source          1..10814
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     regulatory      19..28
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      2260..2269
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(3665..3689)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    3751..4339
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3804..3824)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4222..4233)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(4376..4393)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4382..4606
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4652..5080
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4739..4758)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4949..4970
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5089..5109)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     CDS             7038..7406
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="KLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDDVT
                     LFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGREF
                     ALRDPAGNCVHFVAEEQD"
     polyA_signal    7536..7657
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7573..7592)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7627..7646
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7706..7722)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7706..7722)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7719..7741)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7730..7746
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7754..7760,7761..7778,7779..7784))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7799..7820
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(7937..7954)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8108..8693)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8188..8207)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(8864..9655,9656..9724))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9487..9506
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9725..9829)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(9904..9923)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        10095..10474
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        10475..10678
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     10629..10649
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     protein_bind    10680..10698
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    10701..10719
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     10729..10753
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
ORIGIN
        1 tccgggggat ccactagagc caccatggcc ctctggagac cctccgattc caccgtgtac
       61 ttgccccccc ccagcgtcgc acgcgtcgtg tctaccgacg actacgtcag caggacctca
      121 atcttctacc acgccgggtc cagtaggctg ctgaccgtgg ggaaccccta cttccgcgtc
      181 gtgcccaacg gcgccggcaa caagcaagcc gtccccaaag tcagtgccta ccagtaccgc
      241 gtcttccgcg tggccctgcc agaccccaac aagttcggcc tgcccgacag caccatctac
      301 aaccccgaga cccagaggct cgtctgggcc tgcgtgggca tggagatcgg caggggccaa
      361 cccctgggca tcgggttgtc cgggcacccc ttctacaaca agctcgacga caccgagtcc
      421 gcccacgccg ccaccgccgt catcacccag gacgtccgcg acaacgtcag cgtcgactac
      481 aaacagaccc aactctgcat cctgggctgc gtgcccgcca tcggcgaaca ttgggcaaag
      541 gggaccttgt gcaagcccgc ccagctccag cccggcgatt gcccccccct cgagttgaag
      601 aatacaatca tcgaggacgg cgacatggtc gacaccggct acggcgccat ggacttctcc
      661 accctccaag acaccaaatg tgaagtcccc ctggatatct gccagagtat ttgcaagtac
      721 cccgactacc tccagatgag cgccgaccca tacggcgaca gcatgttctt ctgtttgagg
      781 agggagcagc tcttcgcccg ccacttctgg aaccgcgccg gcgtcatggg cgataccgtg
      841 cccaccgatt tgtacatcaa ggggacctca gccaacatga gggagacacc ggggtcctgc
      901 gtctacagtc ccagcccatc cgggagcatc atcaccagcg acagccagct gttcaacaag
      961 ccctactggc tgcacaaagc acaggggcac aataacggca tctgctggca caaccaactc
     1021 ttcgtcaccg tggtcgatac cacaaggtcc accaacctga ccctgtgcgc aagcacccag
     1081 aaccccgtcc cctccaccta cgatcccacc aagttcaaac agtactcccg ccacgtcgaa
     1141 gagtacgacc tgcagttcat cttccaactc tgtaccatca ccctgaccgc cgaggtcatg
     1201 agctacattc actccatgaa ctcctccatc ctggagaact ggaacttcgg cgtgcccccc
     1261 ccccccacca cctccctcgt cgacacctac aggttcgtcc agagcgtcgc cgtcacatgc
     1321 cagaaggaca ccaccccccc cgagaaacag gacccctacg acaagctgaa gttctggacc
     1381 gtcgatttga aggagaagtt cagtagtgac ctcgaccagt acccattggg caggaaattc
     1441 ctggtccaag ccggcctgag gaggcgcccc acaatcggcc ccaggaagag gcccgccgcc
     1501 agtaccagca ccgccagcac cgccagccgc cccgcaaagc gcgtcaggat caggtccaag
     1561 aaatgagccc ggtggatccc aatcaagctt tttgcaaaag cctagggctc gaggaagctt
     1621 aaaacagctc tggggttgta cccaccccag aggcccacgt ggcggctagt actccggtat
     1681 tgcggtaccc ttgtacgcct gttttatact cccttcccgt aacttagacg cacaaaacca
     1741 agttcaatag aagggggtac aaaccagtac caccacgaac aagcacttct gtttccccgg
     1801 tgatgtcgta tagactgctt gcgtggttga aagcgacgga tccgttatcc gcttatgtac
     1861 ttcgagaagc ccagtaccac ctcggaatct tcgatgcgtt gcgctcagca ctcaacccca
     1921 gagtgtagct taggctgatg agtctggaca tccctcaccg gtgacggtgg tccaggctgc
     1981 gttggcggcc tacctatggc taacgccatg ggacgctagt tgtgaacaag gtgtgaagag
     2041 cctattgagc tacataagaa tcctccggcc cctgaatgcg gctaatccca acctcggagc
     2101 aggtggtcac aaaccagtga ttggcctgtc gtaacgcgca agtccgtggc ggaaccgact
     2161 actttgggtg tccgtgtttc cttttatttt attgtggctg cttatggtga caatcacaga
     2221 ttgttatcat aaagcgaatt ggattgcggc cgctctagag ccaccatggt cagtcatagg
     2281 gccgccagga ggaagagagc aagcgccacc gatctgtacc gcacctgcaa acagagtggc
     2341 acctgtccac ccgacgtcat caataaggtc gaggggacca cactggccga caagatcctg
     2401 caatggagct cattgggcat cttcctcggc gggttgggga tcggcacagg gtccggcagc
     2461 ggcgggagga ccggatacgt gccactgggc gggcgcagca acaccgtcgt cgacgtcggg
     2521 ccaacccgcc cccccgtcgt catcgagccc gtgggcccca ccgaccccag catcgtcacc
     2581 ctcgtggaag acagttccgt cgtcgcaagc ggcgcccccg tcccaacctt caccggcaca
     2641 agcggcttcg agatcaccag cagcggcacc acaacccccg ccgtcctcga tattaccccc
     2701 accgtcgata gcgtcagcat cagcagcacc tccttcacca acccagcctt cagcgaccca
     2761 agcatcatcg aggtcccaca gaccggcgaa gtcagcggca acatcttcgt cggcaccccc
     2821 accagcgggt ctcacggcta cgaagagatc ccactgcaga ccttcgccag cagcggcagc
     2881 ggcaccgagc caatctcctc cacaccattg cccaccgtca gaagagtggc cggcccaagg
     2941 ctctactccc gcgccaacca gcaagtcagg gtcagtacaa gccagttcct gacccaccca
     3001 agcagcctcg tcaccttcga caaccccgcc tacgagccac tcgatacaac cttgagtttc
     3061 gaacccacat ccaacgtccc cgacagtgac ttcatggaca tcatcaggct ccaccgcccc
     3121 gccctgagta gccgcagggg gaccgtccgc ttctcccgcc tcggccagcg cgccacaatg
     3181 ttcaccaggt ccggcaagca gatcggcggc cgcgtgcact tctatcacga catctctcca
     3241 atcgccgcca ccgaagagat cgagctccaa cccctgatct ccgccaccaa cgactccgat
     3301 ctcttcgacg tgtacgccga ttttccgcca cccgccagta ccaccccctc aaccatccat
     3361 aagagcttca cctaccccaa atacagtctc acaatgccca gcaccgccgc cagtagctat
     3421 tccaacgtca ccgtgcccct gaccagcgcc tgggacgtgc ccatctacac cgggcccgat
     3481 atcatcctcc cgagtcacac ccccatgtgg ccctccacca gccccacaaa cgccagtaca
     3541 acaacataca tcggcatcca cgggacccag tactacctgt ggccctggta ctactacttc
     3601 cccaagaaga ggaagaggat cccatacttc ttcgccgacg ggttcgtcgc cgcatgagcc
     3661 cgggacccag ctttcttgta caaagtggtt cgatctagaa tggctagtgg atcccccggg
     3721 ctgcaggaat tcgatatcaa gcttatcgat aatcaacctc tggattacaa aatttgtgaa
     3781 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     3841 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     3901 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3961 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4021 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4081 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4141 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4201 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4261 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     4321 ctttgggccg cctccccgca tcgataccgt cggcccgttt aaacccgctg atcagcctcg
     4381 actgtgcctt ctagttgcca gccatctgtt gtttgcccct cccccgtgcc ttccttgacc
     4441 ctggaaggtg ccactcccac tgtcctttcc taataaaatg aggaaattgc atcgcattgt
     4501 ctgagtaggt gtcattctat tctggggggt ggggtggggc aggacagcaa gggggaggat
     4561 tgggaagaca atagcaggca tgctggggat gcggtgggct ctatggcttc tgaggcggaa
     4621 agaaccagct ggggctctag ggggtatccc cacgcgccct gtagcggcgc attaagcgcg
     4681 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4741 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4801 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4861 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4921 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4981 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5041 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt aattctgtgg aatgtgtgtc
     5101 agttagggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcaga
     5161 attctatcaa atatttaaag aaaaaaaaat tgtatcaact ttctacaatc tctttcagaa
     5221 gacagaagca gagggaatac ttcctaaatc attcaactag gccagcatta ccttaatacc
     5281 ggaactagaa aatgacatta caagaaaaga aaacaacaga ccaatatctc tcatgaacaa
     5341 agatacaaac attttcaaca aaatattagc aaaaagaatc caagaatgta tcaaaaaata
     5401 tacaccacaa ccaagtagaa tttattccag atatgtaagg gtggttcaac gtttgaaaat
     5461 caattaacgt aatttgtccc atcaacaggt taaagaagaa aatcacatgg tcatattgat
     5521 agacacagaa aaagcatttg acaaaattta acacccattc atgatgcaat ctctcagtaa
     5581 actaggaata gaggaaaact tcctcagctt gaatgtacct tcctctcaat tttgctatga
     5641 acctgaaact cctcttaaaa aataaagttt ttcatttaaa aagaaaacaa aaaacatgga
     5701 ggagcgttga tgtatctcat tttagaccaa tcagctatgg atagttaggc gacagcacag
     5761 atagctgctg tacttctgtt tctggcaatg ttccagacta catttaaaaa atttttaatt
     5821 atagacttgt acttaatgtt caagaaaaat atgaaaatgg ctttgccgtg ttaatgctac
     5881 tcttttttaa aaaaaactaa agttcaaact ttatttatat ttcattagtt ttttagctac
     5941 tgttcttttt ctgttctggg atctcattca gaatgccaca ttacatataa ttctcatgtc
     6001 tccttgggtt cctcttagtt ttgacagttc ctcagacttt tcttattttt gatgaccttg
     6061 acagttttga ggagtactgg ttagatatag ggtaatggtt tttaaagtat atttgtcatg
     6121 atttatactg gggtaagggt ttggggagga agcccatggg gtaaagtact gttctcatca
     6181 catcatatca aggttatata ccatcaatat tgccacagat gttacttagc cttttaatat
     6241 ttctctaatt tagtgtatat gcaatgatag ttctctgatt tctgagattg agtttctcat
     6301 gtgtaatgat tatttagagt ttctctttca tctgttcaaa tttttgtcta gttttatttt
     6361 ttactgattt gtaagacttc tttttataat ctgcatatta caattctctt tactggggtg
     6421 ttgcaaatat tttctgtcat tctatggcct gacttttctt aatggttttt taattttaaa
     6481 aataagtctt aatattcatg caatctaatt aacaatcttt tctttgtggt taggactttg
     6541 agtcataaga aatttttctc tacactgaag tcatgatggc atgcttctat attattttct
     6601 aaaagattta aagttttgcc ttctccattt agacttataa ttcactggaa tttttttgtg
     6661 tgtatggtat gacatatggg ttccctttta ttttttacat ataaatatat ttccctgttt
     6721 ttctaaaaaa gaaaaagatc atcattttcc cattgtaaaa tgccatattt ttttcatagg
     6781 tcacttacat atatcaatgg gtctgtttct gagctctact ctattttatc agcctcactg
     6841 tctatcccca cacatctcat gctttgctct aaatcttgat atttagtgga acattctttc
     6901 ccattttgtt ctacaagaat atttttgtta ttgtcttttg ggcttctata tacattttag
     6961 aatgaggttg gcaagttaac aaacagcttt tttggggtga acatattgac tacaaattta
     7021 tgtggaaaga aagtaccaag ttgaccagtg ccgttccggt gctcaccgcg cgcgacgtcg
     7081 ccggagcggt cgagttctgg accgaccggc tcgggttctc ccgggacttc gtggaggacg
     7141 acttcgccgg tgtggtccgg gacgacgtga ccctgttcat cagcgcggtc caggaccagg
     7201 tggtgccgga caacaccctg gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg
     7261 agtggtcgga ggtcgtgtcc acgaacttcc gggacgcctc cgggccggcc atgaccgaga
     7321 tcggcgagca gccgtggggg cgggagttcg ccctgcgcga cccggccggc aactgcgtgc
     7381 acttcgtggc cgaggagcag gactgacacg tgctacgaga tttcgattcc accgccgcct
     7441 tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg atcctccagc
     7501 gcggggatct catgctggag ttcttcgccc accccaactt gtttattgca gcttataatg
     7561 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7621 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctgtata ccgtcgacct
     7681 ctagctagag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     7741 tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     7801 gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     7861 tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     7921 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     7981 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     8041 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     8101 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     8161 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     8221 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     8281 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     8341 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     8401 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     8461 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     8521 ggtggcctaa ctacggctac actagaagaa cagtatttgg tatctgcgct ctgctgaagc
     8581 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     8641 gcggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     8701 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     8761 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     8821 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     8881 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     8941 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     9001 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     9061 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     9121 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     9181 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     9241 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     9301 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     9361 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     9421 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     9481 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     9541 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     9601 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     9661 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     9721 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     9781 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     9841 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     9901 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     9961 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
    10021 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
    10081 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
    10141 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
    10201 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
    10261 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
    10321 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
    10381 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
    10441 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
    10501 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
    10561 tgggagtttg ttttggaacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
    10621 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagagctct
    10681 ccctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag ctcgtttagt
    10741 gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata gaagacaccg
    10801 ggaccgatcc agcc
//
