LOCUS       sequence-469112-        9235 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for regulated phage transfer.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9235)
  AUTHORS   Kusumawardhani, H., Zoppi, F., Avendano, R. et al.
  TITLE     Engineering intercellular communication using M13 phagemid and 
            CRISPR-based gene regulation for multicellular computing in 
            Escherichia coli
  JOURNAL   Nat Commun 16, 3569 (2025)
REFERENCE   2  (bases 1 to 9235)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Commun 
            16, 3569 (2025)"
COMMENT     Sequence Label: HP17deltaP8
FEATURES             Location/Qualifiers
     source          1..9235
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      718..939
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(1431..1525)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     terminator      complement(1681..1710)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     CDS             1921..3153
                     /codon_start=1
                     /gene="II"
                     /product="pII"
                     /label=M13 gene II
                     /note="replication"
                     /translation="MIDMLVLRLPFIDSLVCSRLSGNDLIAFVDLSKIATLSGMNLSAR
                     TVEYHIDGDLTVSGLSHPFESLPTHYSGIAFKIYEGSKNFYPCVEIKASPAKVLQGHNV
                     FGTTDLALCSEALLLNFANSLPCLYDLLDVNATTISRIDATFSARAPNENIAKQVIDHL
                     RNVSNGQTKSTRSQNWESTVTWNETSRHRTLVAYLKHVELQHQIQQLSSKPSAKMTSYQ
                     KEQLKVLSNPDLLEFASGLVRFEARIKTRYLKSFGLPLNLFDAIRFASDYNSQGKDLIF
                     DLWSFSFSELFKAFEGDSMNIYDDSAVLDAIQSKHFTITPSGKTSFAKASRYFGFYRRL
                     VNEGYDSVALTMPRNSFWRYVSALVECGIPKSQLMNLSTCNNVVPLVRFINVDFSSQRP
                     DWYNEPVLKIA"
     CDS             3165..3428
                     /codon_start=1
                     /gene="V"
                     /product="pV"
                     /label=M13 gene V
                     /note="replication"
                     /translation="MIKVEIKPSQAQFTTRSGVSRQGKPYSLNEQLCYVDLGNEYPVLV
                     KITLDEGQPAYAPGLYTVHLSSFKVGQFGSLMIDRLRLVPAK"
     CDS             3430..3531
                     /codon_start=1
                     /gene="VII"
                     /product="pVII"
                     /label=M13 gene VII
                     /note="minor coat protein"
                     /translation="MEQVADFDTIYQAMIQISVVLCFALGIIAGGQR"
     CDS             3528..3626
                     /codon_start=1
                     /gene="IX"
                     /product="pIX"
                     /label=M13 gene IX
                     /note="minor coat protein"
                     /translation="MSVLVYSFASFVLGWCLRSGITYFTRLMETSS"
     terminator      3635..3683
                     /label=fd terminator
                     /note="central terminator from bacteriophage fd (Otsuka and
                     Kunisawa, 1982)"
     CDS             3688..4962
                     /codon_start=1
                     /gene="III"
                     /product="pIII"
                     /label=M13 gene III
                     /note="minor coat protein"
                     /translation="MKKLLFAIPLVVPFYSHSAETVESCLAKPHTENSFTNVWKDDKTL
                     DRYANYEGCLWNATGVVVCTGDETQCYGTWVPIGLAIPENEGGGSEGGGSEGGGSEGGG
                     TKPPEYGDTPIPGYTYINPLDGTYPPGTEQNPANPNPSLEESQPLNTFMFQNNRFRNRQ
                     GALTVYTGTVTQGTDPVKTYYQYTPVSSKAMYDAYWNGKFRDCAFHSGFNEDPFVCEYQ
                     GQSSDLPQPPVNAGGGSGGGSGGGSEGGGSEGGGSEGGGSEGGGSGGGSGSGDFDYEKM
                     ANANKGAMTENVDENALQSDAKGKLDSVATDYGAAIDGFIGDVSGLANGNGATGDFAGS
                     NSQMAQVGDGDNSPLMNNFRQYLPSLPQSVECRPFVFGAGKPYEFSIDCDKINLFRGVF
                     AFLLYVATFMYVFSTFANILRNKES"
     CDS             4965..5303
                     /codon_start=1
                     /gene="VI"
                     /product="pVI"
                     /label=M13 gene VI
                     /note="minor coat protein"
                     /translation="MPVLLGIPLLLRFLGFLLVTLFGYLLTFLKKGFGKIAIAISLFLA
                     LIIGLNSILVGYLSDISAQLPSDFVQGVQLILPSNALPCFYVILSVKAAIFIFDVKQKI
                     VSYLDWDK"
     CDS             5305..6351
                     /codon_start=1
                     /gene="I"
                     /product="pI"
                     /label=M13 gene I
                     /note="phage assembly"
                     /translation="MAVYFVTGKLGSGKTLVSVGKIQDKIVAGCKIATNLDLRLQNLPQ
                     VGRFAKTPRVLRIPDKPSISDLLAIGRGNDSYDENKNGLLVLDECGTWFNTRSWNDKER
                     QPIIDWFLHARKLGWDIIFLVQDLSIVDKQARSALAEHVVYCRRLDRITLPFVGTLYSL
                     ITGSKMPLPKLHVGVVKYGDSQLSPTVERWLYTGKNLYNAYDTKQAFSSNYDSGVYSYL
                     TPYLSHGRYFKPLNLGQKMKLTKIYLKKFSRVLCLAIGFASAFTYSYITQPKPEVKKVV
                     SQTYDFDKFTIDSSQRLNLSYRYVFKDSKGKLINSDDLQKQGYSLTYIDLCTVSIKKGN
                     SNEIVKCN"
     CDS             6329..7609
                     /codon_start=1
                     /gene="IV"
                     /product="pIV"
                     /label=M13 gene IV
                     /note="phage assembly"
                     /translation="MKLLNVINFVFLMFVSSSSFAQVIEMNNSPLRDFVTWYSKQSGES
                     VIVSPDVKGTVTVYSSDVKPENLRNFFISVLRANNFDMVGSNPSIIQKYNPNNQDYIDE
                     LPSSDNQEYDDNSAPSGGFFVPQNDNVTQTFKINNVRAKDLIRVVELFVKSNTSKSSNV
                     LSIDGSNLLVVSAPKDILDNLPQFLSTVDLPTDQILIEGLIFEVQQGDALDFSFAAGSQ
                     RGTVAGGVNTDRLTSVLSSAGGSFGIFNGDVLGLSVRALKTNSHSKILSVPRILTLSGQ
                     KGSISVGQNVPFITGRVTGESANVNNPFQTIERQNVGISMSVFPVAMAGGNIVLDITSK
                     ADSLSSSTQASDVITNQRSIATTVNLRDGQTLLLGGLTDYKNTSQDSGVPFLSKIPLIG
                     LLFSSRSDSNEESTLYVLVKATIVRAL"
     terminator      7652..7683
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     CDS             complement(7782..8597)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     8505..8524
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     8589..8608
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             complement(join(8955..9235,1..670))
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFGIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLHNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKYDLNGSFSWLTQKQRTTLENILAKYGRI"
ORIGIN
        1 gttcaacttg gaaaatcaac gtatcagtcg ggcggcctcg cttatcaacc accaatttca
       61 tattgctgta agtgtttaaa tctttactta ttggtttcaa aacccattgg ttaagccttt
      121 taaactcatg gtagttattt tcaagcatta acatgaactt aaattcatca aggctaatct
      181 ctatatttgc cttgtgagtt ttcttttgtg ttagttcttt taataaccac tcataaatcc
      241 tcatagagta tttgttttca aaagacttaa catgttccag attatatttt atgaattttt
      301 ttaactggaa aagataaggc aatatctctt cactaaaaac taattctaat ttttcgcttg
      361 agaacttggc atagtttgtc cactggaaaa tcccaaagcc tttaaccaaa ggattcctga
      421 tttccacagt tctcgtcatc agctctctgg ttgctttagc taatacacca taagcatttt
      481 ccctactgat gttcatcatc tgagcgtatt ggttataagt gaacgatacc gtccgttctt
      541 tccttgtagg gttttcaatc gtggggttga gtagtgccac acagcataaa attagcttgg
      601 tttcatgctc cgttaagtca tagcgactaa tcgctagttc atttgctttg aaaacaacta
      661 attcagacat acatctcaat tggtctaggt gattttaatc actataccaa ttgagatggg
      721 ctagtcaatg ataattacat gtccttttcc tttgagttgt gggtatctgt aaattctgct
      781 agacctttgc tggaaaactt gtaaattctg ctagaccctc tgtaaattcc gctagacctt
      841 tgtgtgtttt tttgtttata ttcaagtggt tataatttat agaataaaga aagaataaaa
      901 aaagataaaa agaatagatc ccagccctgt gtataactca ctactttagt cagttccgca
      961 gtattacaaa aggatgtcgc aaacgctgtt tgctcctcta caaaacagac cttaaaaccc
     1021 taaaggctta agtagcaccc tcgcaagctc gggcaaatcg ctgaatattc cttttgtctc
     1081 cgaccatcag gcacctgagt cgctgtcttt ttcgtgacat tcagttcgct gcgctcacgg
     1141 ctctggcagt gaatgggggt aaatggcact acaggcgcct tttatggatt catgcaagga
     1201 aactacccat aatacaagaa aagcccgtca cgggcttctc agggcgtttt atggcgggtc
     1261 tgctatgtgg tgctatctga ctttttgctg ttcagcagtt cctgccctct gattttccag
     1321 tctgaccact tcggattatc ccgtgacagg tcattcagac tggctaatgc acccagtaag
     1381 gcagcggtat catcaacagg cttacccgtc ttactgtccc tagtgcttgg attctcacca
     1441 ataaaaaacg cccggcggca accgagcgtt ctgaacaaat ccagatggag ttctgaggtc
     1501 attactggat ctatcaacag gagtccaagc gagctcgtaa acttggtctg acagctctag
     1561 ctccggcaaa aaaacgggca aggtgtcacc accctgccct ttttctttaa aaccgaaaag
     1621 attacttcgc gtttgccacc tgacgtctaa gaaaaggaat attcagcaat ttgcccgtgc
     1681 cgaagaaagg cccacccgtg aaggtgagcc agtgagttga ttgctacgta attagttagt
     1741 tagcccttag tgactccaga cgcgaattat ttttgatggc gttcctattg gttaaaaaat
     1801 gagctgattt aacaaaaatt taatgcgaat tttaacaaaa tattaacgtt tacaatttaa
     1861 atatttgctt atacaatctt cctgtttttg gggcttttct gattatcaac cggggtacat
     1921 atgattgaca tgctagtttt acgattaccg ttcatcgatt ctcttgtttg ctccagactc
     1981 tcaggcaatg acctgatagc ctttgtagac ctctcaaaaa tagctaccct ctccggcatg
     2041 aatttatcag ctagaacggt tgaatatcat attgatggtg atttgactgt ctccggcctt
     2101 tctcaccctt ttgaatcttt acctacacat tactcaggca ttgcatttaa aatatatgag
     2161 ggttctaaaa atttttatcc ttgcgttgaa ataaaggctt ctcccgcaaa agtattacag
     2221 ggtcataatg tttttggtac aaccgattta gctttatgct ctgaggcttt attgcttaat
     2281 tttgctaatt ctttgccttg cctgtatgat ttattggatg ttaacgctac tactattagt
     2341 agaattgatg ccaccttttc agctcgcgcc ccaaatgaaa atatagctaa acaggttatt
     2401 gaccatttgc gaaatgtatc taatggtcaa actaaatcta ctcgttcgca gaattgggaa
     2461 tcaactgtta catggaatga aacttccaga caccgtactt tagttgcata tttaaaacat
     2521 gttgagctac agcaccagat tcagcaatta agctctaagc catccgcaaa aatgacctct
     2581 tatcaaaagg agcaattaaa ggtactctct aatcctgacc tgttggagtt tgcttccggt
     2641 ctggttcgct ttgaagctcg aattaaaacg cgatatttga agtctttcgg gcttcctctt
     2701 aatctttttg atgcaatccg ctttgcttct gactataata gtcagggtaa agacctgatt
     2761 tttgatttat ggtcattctc gttttctgaa ctgtttaaag catttgaggg ggattcaatg
     2821 aatatttatg acgattccgc agtattggac gctatccagt ctaaacattt tactattacc
     2881 ccctctggca aaacttcttt tgcaaaagcc tctcgctatt ttggttttta tcgtcgtctg
     2941 gtaaacgagg gttatgatag tgttgctctt actatgcctc gtaattcctt ttggcgttat
     3001 gtatctgcat tagttgaatg tggtattcct aaatctcaac tgatgaatct ttctacctgt
     3061 aataatgttg ttccgttagt tcgttttatt aacgtagatt tttcttccca acgtcctgac
     3121 tggtataatg agccagttct taaaatcgca taaggtaatt cacaatgatt aaagttgaaa
     3181 ttaaaccatc tcaagcccaa tttactactc gttctggtgt ttctcgtcag ggcaagcctt
     3241 attcactgaa tgagcagctt tgttacgttg atttgggtaa tgaatatccg gttcttgtca
     3301 agattactct tgatgaaggt cagccagcct atgcgcctgg tctgtacacc gttcatctgt
     3361 cctctttcaa agttggtcag ttcggttccc ttatgattga ccgtctgcgc ctcgttccgg
     3421 ctaagtaaca tggagcaggt cgcggatttc gacacaattt atcaggcgat gatacaaatc
     3481 tccgttgtac tttgtttcgc gcttggtata atcgctgggg gtcaaagatg agtgttttag
     3541 tgtattcttt cgcctctttc gttttaggtt ggtgccttcg tagtggcatt acgtatttta
     3601 cccgtttaat ggaaacttcc tcatgagctg ataaaccgat acaattaaag gctccttttg
     3661 gagccttttt ttttggagat tttcaacgtg aaaaaattat tattcgcaat tcctttagtt
     3721 gttcctttct attctcactc cgctgaaact gttgaaagtt gtttagcaaa accccataca
     3781 gaaaattcat ttactaacgt ctggaaagac gacaaaactt tagatcgtta cgctaactat
     3841 gagggctgtc tgtggaatgc tacaggcgtt gtagtttgta ctggtgacga aactcagtgt
     3901 tacggtacat gggttcctat tgggcttgct atccctgaaa atgagggtgg tggctctgag
     3961 ggtggcggtt ctgagggtgg cggttctgag ggtggcggta ctaaacctcc tgagtacggt
     4021 gatacaccta ttccgggcta tacttatatc aaccctctcg acggcactta tccgcctggt
     4081 actgagcaaa accccgctaa tcctaatcct tctcttgagg agtctcagcc tcttaatact
     4141 ttcatgtttc agaataatag gttccgaaat aggcaggggg cattaactgt ttatacgggc
     4201 actgttactc aaggcactga ccccgttaaa acttattacc agtacactcc tgtatcatca
     4261 aaagccatgt atgacgctta ctggaacggt aaattcagag actgcgcttt ccattctggc
     4321 tttaatgagg atccattcgt ttgtgaatat caaggccaat cgtctgacct gcctcaacct
     4381 cctgtcaatg ctggcggcgg ctctggtggt ggttctggtg gcggctctga gggtggtggc
     4441 tctgagggtg gcggttctga gggtggcggc tctgagggag gcggttccgg tggtggctct
     4501 ggttccggtg attttgatta tgaaaagatg gcaaacgcta ataagggggc tatgaccgaa
     4561 aatgtcgatg aaaacgcgct acagtctgac gctaaaggca aacttgattc tgtcgctact
     4621 gattacggtg ctgctatcga tggtttcatt ggtgacgttt ccggccttgc taatggtaat
     4681 ggtgctactg gtgattttgc tggctctaat tcccaaatgg ctcaagtcgg tgacggtgat
     4741 aattcacctt taatgaataa tttccgtcaa tatttacctt ccctccctca atcggttgaa
     4801 tgtcgccctt ttgtctttgg cgctggtaaa ccatatgaat tttctattga ttgtgacaaa
     4861 ataaacttat tccgtggtgt ctttgcgttt cttttatatg ttgccacctt tatgtatgta
     4921 ttttctacgt ttgctaacat actgcgtaat aaggagtctt aatcatgcca gttcttttgg
     4981 gtattccgtt attattgcgt ttcctcggtt tccttctggt aactttgttc ggctatctgc
     5041 ttacttttct taaaaagggc ttcggtaaga tagctattgc tatttcattg tttcttgctc
     5101 ttattattgg gcttaactca attcttgtgg gttatctctc tgatattagc gctcaattac
     5161 cctctgactt tgttcagggt gttcagttaa ttctcccgtc taatgcgctt ccctgttttt
     5221 atgttattct ctctgtaaag gctgctattt tcatttttga cgttaaacaa aaaatcgttt
     5281 cttatttgga ttgggataaa taatatggct gtttattttg taactggcaa attaggctct
     5341 ggaaagacgc tcgttagcgt tggtaagatt caggataaaa ttgtagctgg gtgcaaaata
     5401 gcaactaatc ttgatttaag gcttcaaaac ctcccgcaag tcgggaggtt cgctaaaacg
     5461 cctcgcgttc ttagaatacc ggataagcct tctatatctg atttgcttgc tattgggcgc
     5521 ggtaatgatt cctacgatga aaataaaaac ggcttgcttg ttctcgatga gtgcggtact
     5581 tggtttaata cccgttcttg gaatgataag gaaagacagc cgattattga ttggtttcta
     5641 catgctcgta aattaggatg ggatattatt tttcttgttc aggacttatc tattgttgat
     5701 aaacaggcgc gttctgcatt agctgaacat gttgtttatt gtcgtcgtct ggacagaatt
     5761 actttacctt ttgtcggtac tttatattct cttattactg gctcgaaaat gcctctgcct
     5821 aaattacatg ttggcgttgt taaatatggc gattctcaat taagccctac tgttgagcgt
     5881 tggctttata ctggtaagaa tttgtataac gcatatgata ctaaacaggc tttttctagt
     5941 aattatgatt ccggtgttta ttcttattta acgccttatt tatcacacgg tcggtatttc
     6001 aaaccattaa atttaggtca gaagatgaaa ttaactaaaa tatatttgaa aaagttttct
     6061 cgcgttcttt gtcttgcgat tggatttgca tcagcattta catatagtta tataacccaa
     6121 cctaagccgg aggttaaaaa ggtagtctct cagacctatg attttgataa attcactatt
     6181 gactcttctc agcgtcttaa tctaagctat cgctatgttt tcaaggattc taagggaaaa
     6241 ttaattaata gcgacgattt acagaagcaa ggttattcac tcacatatat tgatttatgt
     6301 actgtttcca ttaaaaaagg taattcaaat gaaattgtta aatgtaatta attttgtttt
     6361 cttgatgttt gtttcatcat cttcttttgc tcaggtaatt gaaatgaata attcgcctct
     6421 gcgcgatttt gtaacttggt attcaaagca atcaggcgaa tccgttattg tttctcccga
     6481 tgtaaaaggt actgttactg tatattcatc tgacgttaaa cctgaaaatc tacgcaattt
     6541 ctttatttct gttttacgtg caaataattt tgatatggta ggttctaacc cttccattat
     6601 tcagaagtat aatccaaaca atcaggatta tattgatgaa ttgccatcat ctgataatca
     6661 ggaatatgat gataattccg ctccttctgg tggtttcttt gttccgcaaa atgataatgt
     6721 tactcaaact tttaaaatta ataacgttcg ggcaaaggat ttaatacgag ttgtcgaatt
     6781 gtttgtaaag tctaatactt ctaaatcctc aaatgtatta tctattgacg gctctaatct
     6841 attagttgtt agtgctccta aagatatttt agataacctt cctcaattcc tttcaactgt
     6901 tgatttgcca actgaccaga tattgattga gggtttgata tttgaggttc agcaaggtga
     6961 tgctttagat ttttcatttg ctgctggctc tcagcgtggc actgttgcag gcggtgttaa
     7021 tactgaccgc ctcacctctg ttttatcttc tgctggtggt tcgttcggta tttttaatgg
     7081 cgatgtttta gggctatcag ttcgcgcatt aaagactaat agccattcaa aaatattgtc
     7141 tgtgccacgt attcttacgc tttcaggtca gaagggttct atctctgttg gccagaatgt
     7201 cccttttatt actggtcgtg tgactggtga atctgccaat gtaaataatc catttcagac
     7261 gattgagcgt caaaatgtag gtatttccat gagcgttttt cctgttgcaa tggctggcgg
     7321 taatattgtt ctggatatta ccagcaaggc cgatagtttg agttcttcta ctcaggcaag
     7381 tgatgttatt actaatcaaa gaagtattgc tacaacggtt aatttgcgtg atggacagac
     7441 tcttttactc ggtggcctca ctgattataa aaacacttct caggattctg gcgtaccgtt
     7501 cctgtctaaa atccctttaa tcggcctcct gtttagctcc cgctctgatt ctaacgagga
     7561 aagcacgtta tacgtgctcg tcaaagcaac catagtacgc gccctgtagc atgcaggagt
     7621 cactaagggt tagttagtta gattagcaga aagtcaaaag cctccgaccg gaggcttttg
     7681 actaaaactt cccttggggt tatcattggg gctcactcaa aggcggtaat cagataaaaa
     7741 aaatccttag ctttcgctaa ggatgatttc tgctagtatt attagaaaaa ctcatcgagc
     7801 atcaaatgaa actgcaattt attcatatca ggattatcaa taccatattt ttgaaaaagc
     7861 cgtttctgta atgaaggaga aaactcaccg aggcagttcc aaagaatggc aaggtcctgg
     7921 taacggtctg cgattccgac ccgtccaaca tcaatacaac ctattaattt cccctcgtca
     7981 aaaataaggt tatcaagtga gaaatcacca tgagtgacga ctgaatccgg tgagaatggc
     8041 aagagcttgt gcatttcttt ccagacttgt tcaacaggcc agccattacg ctcgtcatca
     8101 aaatcactcg catcaaccaa accgttattc atgcgtgatt gcgcctgagc aagacgaaat
     8161 acacgatcgc tgttaaaagg acaattacaa acaggaatcg aatgtaaccg gcgcaggaac
     8221 acggccagcg catcaacaat attttcacct gaatcaggat attcttctaa tacctggaag
     8281 gctgttttcc caggaatcgc ggtggtgagt aaccacgcat catcaggagt acggataaaa
     8341 tgcttgatgg tcgggagagg cataaactcc gtcagccagt tgagacggac catctcatct
     8401 gtaacatcat tggcaacgct acctttgcca tgtttcagaa acaactctgg cgcatcgggc
     8461 ttcccataca agcgatagat tgtcgcacct gattgcccga cattatcgcg agcccattta
     8521 tacccatata aatcagcgtc catgttggag tttaagcgcg gacgggagca agacgtttcc
     8581 cgttgaatat ggctcataac accccttgta ttactgttta tgtaagcaga cagttttatt
     8641 gttcatgatg atatattttt atcttgtgca atgtaacatc agagattttg agacacaacg
     8701 tggctttgtt gaataaatcg aacttttgct gagttgaagg atcagctcta gtagttacat
     8761 tgtcgatctg ttcatggtga acagctttga atgcaccaaa aactcgtaaa agctctgatg
     8821 tatctatctt ttttacaccg ttttcatctg tgcatatgga cagttttccc tttgatatgt
     8881 aacggtgaac agttgttcta cttttgtttg ttagtcttga tgcttcactg atagatacaa
     8941 gagccataag aacctcagat ccttccgtat ttagccagta tgttctctag tgtggttcgt
     9001 tgtttttgcg tgagccatga gaacgaacca ttgagatcat acttactttg catgtcactc
     9061 aaaaattttg cctcaaaact ggtgagctga atttttgcag ttaaagcatc gtgtagtgtt
     9121 tttcttagtc cgttatgtag gtaggaatct gatgtaatgg ttgttggtat tttgtcacca
     9181 ttcattttta tctggttgtt ctcaagttcg gttacgagat ccatttgtct atcta
//
