LOCUS       sequence-469117-       14116 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  FLP Recombinase switch; plant T-DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14116)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 14116)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC432
FEATURES             Location/Qualifiers
     source          1..14116
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        542..887
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     830..850
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    1018..1051
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             1053..1850
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1110..1129)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1720..1739
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1860..2108
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     protein_bind    2171..2204
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             2246..6196
                     /label=RUBY
     CDS             3746..3802
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4637..4693
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    6213..6387
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6519..6543
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6968..7762
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9187..9775
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9676..9695
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9929..9946
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    9961..10101
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10027..10046)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10187..10209
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10445..10639
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10705..11778)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12207..12836)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttt
       61 tcgtacgacc ctcggtaccg atcggcgcgc cggagagatc gcgcacgcca atgctaccgt
      121 aggtcctgct tcaggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg
      181 tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt
      241 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      301 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
      361 atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      421 aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgataac atggtggagc
      481 acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta
      541 ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta
      601 tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt
      661 gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac
      721 ccccacccac gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag
      781 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      841 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tcgagtataa
      901 gagctcattt ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat
      961 ttacaattat cgatacaatc tgagaacacg acgctgagct cggacggcgc gccggaggaa
     1021 gttcctattc tctagaaagt ataggaactt caatggggat tgaacaagat ggattgcacg
     1081 caggttctcc ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa
     1141 tcggctgctc tgatgccgcc gtgtttcggc tgtcagcgca ggggcgcccg gttctttttg
     1201 tcaagaccga cctgtccggt gccctgaatg aactccagga cgaggcagcg cggctatcgt
     1261 ggctggccac gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa
     1321 gggactggct gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc
     1381 ctgccgagaa agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg
     1441 ctacctgccc attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg
     1501 aagccggtct tgtcgatcag gatgatctgg acgaagaaca tcaggggctc gcgccagccg
     1561 aactgttcgc caggctcaag gcgcgcatgc ccgacggcga ggatctcgtc gtgacacatg
     1621 gcgatgcctg cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact
     1681 gtggccggct gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg
     1741 ctgaagaact tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc
     1801 ccgattcgca gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga gcgggcttat
     1861 atgaagatga agatgaaata tttggtgtgt caaataaaaa gcttgtgtgc ttaagtttgt
     1921 gtttttttct tggcttgttg tgttatgaat ttgtggcttt ttctaatatt aaatgaatgt
     1981 aagatcacat tataatgaat aaacaaatgt ttctataatc cattgtgaat gttttgttgg
     2041 atctcttctg cagcatataa ctactgtatg tgctatggta tggactatgg aatatgatta
     2101 aagataagcg ctgagctccc ggggcgcgcc ggagagatcg cgcacgccaa tgctaccgta
     2161 ggtcctgctt gaagttccta ttctctagaa agtataggaa cttccgctga gctctcccgg
     2221 cgcgccggag agatcgcgca cgccaatgga tcatgcgacc ctcgccatga tcctcgcgat
     2281 ctggttcatc agcttccact tcatcaagct gctgttctcc cagcagacca ccaagctgct
     2341 tccgccagga ccaaagccgc ttccgatcat cggcaacatc cttgaggtgg gcaagaagcc
     2401 gcatcggtcc ttcgccaacc tcgccaagat tcacggccca ctcatttccc tcagactcgg
     2461 ctctgtgacc accatcgttg tgtcctctgc cgacgtggcc aaagagatgt tcctcaagaa
     2521 ggatcacccg ctctccaacc gcacgatccc gaatagtgtt acagccggcg accaccacaa
     2581 gctcaccatg tcttggctcc cggtgtctcc gaagtggcgc aacttccgca agattaccgc
     2641 cgtgcatctg ctctccccac agagactcga tgcctgccag acattcaggc acgccaaggt
     2701 gcagcagctc tacgagtacg ttcaagagtg cgcccagaaa ggccaggccg tggatattgg
     2761 caaggccgcc tttacgacca gcctcaacct cctcagcaag ctgttcttca gcgtcgagct
     2821 ggcgcaccac aagtcccata ccagccaaga gttcaaagag ctgatctgga acatcatgga
     2881 agatataggc aagccgaact acgccgacta cttcccgatt ctcggctgcg ttgacccatc
     2941 tggcattaga agaaggctcg cctgctcctt cgacaagctg atcgccgtgt tccagggcat
     3001 catctgcgag agactcgccc cagattcctc caccacaact accaccacca ccgacgacgt
     3061 gctcgatgtg ctcctccagc tgttcaagca gaacgagctg acgatgggcg agatcaacca
     3121 cctcctcgtg gacatcttcg acgccggcac cgataccaca tcctccacat tcgagtgggt
     3181 gatgaccgag ctgatccgca atccagagat gatggaaaag gcccaagagg aaatcaagca
     3241 ggtcctcggc aaggacaagc agatccaaga gtccgacatc atcaacctgc cgtacctcca
     3301 ggcgatcatc aaagagacac tccgcctcca tccgccgacc gtgttcttgc tcccaagaaa
     3361 ggccgacacc gatgtcgagc tgtacggcta catcgtgccg aaggatgccc agatcctcgt
     3421 gaacctctgg gccattggca gggacccaaa cgcctggcag aacgccgata ttttcagccc
     3481 agagcgcttc atcggctgcg agatcgatgt taagggccgc gatttcggcc tccttccatt
     3541 tggcgctggc cgcagaattt gcccaggcat gaatctcgcc atcaggatgc tcaccctcat
     3601 gctcgccaca ctcctccagt tcttcaactg gaagctcgaa ggcgacatct ccccgaagga
     3661 cctcgacatg gacgagaagt tcggcattgc gctccaaaag accaagccgc tcaagctcat
     3721 cccgattccg cgctacggta gcggagctac caattttagc ctccttaagc aggctggtga
     3781 tgtagaagag aaccccgggc ctatgaagat gatgaacggc gaggacgcca acgaccagat
     3841 gatcaaagag tccttcttca tcacccacgg caacccgatc ctcaccgtcg aggatacaca
     3901 tccgctcagg ccgttcttcg agacatggcg cgagaagatt ttctccaaga agccgaaggc
     3961 catcctcatc atctccggcc actgggagac agtgaagcca accgtgaacg ccgtgcacat
     4021 caacgacacc atccacgact tcgacgacta cccagccgcc atgtaccagt tcaagtaccc
     4081 agctccaggc gagccagagc ttgcgagaaa ggtggaagag atcctcaaga agtccgggtt
     4141 cgagacagcc gagacagacc aaaagagggg ccttgatcac ggcgcctggg ttccactcat
     4201 gctcatgtat ccagaggcgg acatcccggt gtgccagctc tcagttcagc cacatctcga
     4261 cggcacctac cactacaatc tcggcagagc cctcgcgccg ctcaagaatg atggcgtgct
     4321 cattattggc tccggcagcg ccacacatcc actcgatgag acaccgcact acttcgatgg
     4381 tgttgcccct tgggccgctg ccttcgattc ttggcttagg aaggccctca tcaacggccg
     4441 cttcgaggaa gtgaacatct acgagagcaa ggccccgaac tggaagctcg cccatccatt
     4501 tccagagcac ttctacccgc tccacgttgt gctcggcgct gctggtgaaa agtggaaggc
     4561 cgagctgatc cactcctcct gggatcatgg cacactttgc cacggctcct acaagttcac
     4621 ctccgccgga tccggagcaa ccaactttag cctgctcaag caagcaggag atgttgagga
     4681 aaatcctggc cccatgaccg ccatcaagat gaacaccaac ggcgagggcg agacacagca
     4741 catcctcatg atcccgttca tggcgcaggg ccacctcagg ccatttctcg aactcgccat
     4801 gttcctctac aagcgctccc acgtgatcat caccctgctc acaactccgc tcaatgccgg
     4861 cttcctcagg cacctccttc accaccattc ctactcctcc agcggcatca ggatcgtcga
     4921 gctgccattc aactccacca accacggact cccaccgggc atcgagaaca ccgataagct
     4981 cacactcccg ctcgtggtgt ccctcttcca ttccaccatc agcctcgatc cgcacctccg
     5041 cgattacatc tccaggcatt tcagcccagc caggccacca ctctgcgtga tccatgatgt
     5101 gttcctcggc tgggttgacc aggtggccaa ggatgtgggc tctacaggcg tggtgttcac
     5161 aacaggcggc gcttatggca catccgccta cgtgtccatc tggaacgatc tcccgcacca
     5221 gaactactcc gacgaccaag agttcccgct gccaggcttc ccagagaacc ataagttccg
     5281 caggtcccag ctccatcggt tcctcagata tgccgacggc tccgacgatt ggtccaagta
     5341 tttccagccg cagctccgcc agtccatgaa gtcttttggc tggctctgca actccgtgga
     5401 agagatcgag acactcggct tctccatcct ccgcaactac accaagctgc cgatctgggg
     5461 catcggccca cttattgctt ccccagtgca gcactcctcc tccgacaaca attcaacagg
     5521 cgccgagttc gtgcagtggc tcagcctcaa agagccggac tccgtcctct acatctcctt
     5581 cggctcccag aacacgatca gcccgacgca gatgatggaa ctcgctgctg gccttgagtc
     5641 ctccgagaag ccattcctct gggtgatcag agccccgttc ggcttcgaca tcaacgaaga
     5701 gatgcgccca gagtggctgc cagagggctt tgaggaacgc atgaaggtga agaaacaggg
     5761 caagctcgtg tacaagctcg gcccgcagct tgagatcctc aaccatgaat ccatcggcgg
     5821 ctttctcacc cactgcggat ggaacagcat ccttgagtct cttcgcgagg gcgttccgat
     5881 gcttggatgg ccacttgctg ccgagcaggc ctacaacctc aagtacctcg aagatgagat
     5941 gggcgtcgcg gttgagcttg ctagaggcct cgaaggcgag atctccaaag agaaggtcaa
     6001 gcgcatcgtc gagatgatcc ttgagcgcaa cgagggctcc aaaggctggg agatgaagaa
     6061 tcgcgccgtg gaaatgggca aaaagctcaa ggacgccgtg aacgaggaaa aagagctgaa
     6121 gggctcctcc gtgaaggcga tcgacgattt cctcgacgcc gtcatgcagg ccaaacttga
     6181 gccaagcctc cagtgatagt gagcttctcg agtttctcca taataatgtg tgagtagttc
     6241 ccagataagg gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac
     6301 ccttagtatg tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa
     6361 ccaaaatcca gtactaaaat ccagatcccc cgaattacgc tgagctcagt gtttgatcgc
     6421 cggcggtacc gagtgtactt caagtcactc aattcggcgt taattcagta cattaaaaac
     6481 gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg
     6541 ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca
     6601 ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg
     6661 ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga
     6721 tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc
     6781 accgcggttt caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact
     6841 ttgaaaaagc tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt
     6901 tcgtcttgtt ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa
     6961 ataataaatg gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg
     7021 ctgcgtaaaa gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa
     7081 tgaaaaccta tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga
     7141 acgggaaaag gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca
     7201 ctttgaacgg catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg
     7261 ctcggaagag tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg
     7321 catcaggctc tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag
     7381 ccgcttagcc gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa
     7441 ctgggaagaa gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga
     7501 aaagcccgaa gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt
     7561 gaaagatggc aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg
     7621 gtatgacatt gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt
     7681 cgagctattt tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat
     7741 tttactggat gaattgtttt agtacctaga atgcatgacc aaaatccctt aacgctgatg
     7801 aatcccctaa tgattttggt aaaaatcatt aagttaaggt ggatacacat cttgtcatat
     7861 gatcaaatgg tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa ctcgatattt
     7921 tacacgactc tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa
     7981 cgttggcttg ccacgcatta cttgactgta aaactctcac tcttaccgaa cttggccgta
     8041 acctgccaac caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta
     8101 atcgtcacct ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt
     8161 cgggcaatac gatgcccatt gtacttgttg actggtctga tattcgtgag caaaaacgac
     8221 ttatggtatt gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag
     8281 cgttcccgct ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg
     8341 cgagcattct accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc
     8401 catggtataa atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag
     8461 tacaatatgc agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt
     8521 catctagtca ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat
     8581 gccaaattct attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga
     8641 ctcattgtca ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatgggttc
     8701 tagcaactaa cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga
     8761 agcgaatgca gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc
     8821 tacgccatag ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga
     8881 tgcttcaact aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc
     8941 acttccaggc taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg
     9001 aagttttgcg gcattctggc tacacaataa caagggaaga cttactcgtg gctgcaaccc
     9061 tactagctca aaatttattc acacatggtt acgctttggg gaaattatga ggggatctct
     9121 cagcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     9181 atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     9241 gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     9301 tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     9361 ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     9421 ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     9481 ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     9541 aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     9601 cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     9661 gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     9721 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     9781 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     9841 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     9901 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg
     9961 cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac
    10021 tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta
    10081 cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg
    10141 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtttccgg gagctgcatg
    10201 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg
    10261 ccggcggtcg agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag
    10321 gccagccatt tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag
    10381 ggagcgcagc gaccgaaggg taggcgcttt ttgcagctct tgggctgtgc gctggccaga
    10441 cagttatgca caggccaggc gggttttaag agttttaata agttttaaag agttttaggc
    10501 ggaaaaatcg ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat
    10561 gtacggcttt gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc
    10621 aatgtacgtg ctatccacag gaaagaggcc ttttcgacct ttttcccctg ctagggcaat
    10681 ttgccctagc atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt
    10741 gcggtagcgc atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc
    10801 cggcaggtca tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc
    10861 ggcgctgcca ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc
    10921 ggcgcggcgt gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc
    10981 ggggtgaacc gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc
    11041 agctagctcg atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg
    11101 gctaatcaag gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt
    11161 acgctgcatg gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt
    11221 ctgctttccg ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa
    11281 cacgcggccg ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg
    11341 ggaaaccgcc atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc
    11401 ggaaacctct acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc
    11461 acgcttcgac agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac
    11521 gtcatagagc atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat
    11581 cgacggcgca ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc
    11641 ttgccacgat tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc
    11701 ggccttcaac ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga
    11761 tggtttgcga ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc
    11821 cggcaggcaa cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat
    11881 tccacggcgt cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa
    11941 attcatctac tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga
    12001 tagcagctcg gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc
    12061 ctccgccggc aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa
    12121 cgttgcagcc ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt
    12181 gctcattttc tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc
    12241 gcctggacct cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg
    12301 gcggcagtgc ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg
    12361 tacccggcca gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac
    12421 acgacaaagg ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc
    12481 accaggtcgg cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag
    12541 tcggctgcct tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact
    12601 acgaagtcgc gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg
    12661 acaacggtta gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga
    12721 tcggaatcga ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt
    12781 gcgatggtcg tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt
    12841 tccccttgcg tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca
    12901 agctgtttta ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg
    12961 ccaagctggc cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca
    13021 gccgcacggt tgagccgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg
    13081 cctcgatctc ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc
    13141 ttgttcctct tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc
    13201 ctcacggaag gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag
    13261 tgcgcggtac agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg
    13321 gccttcctgg tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg
    13381 ggggccaaac ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat
    13441 gattagggaa cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc
    13501 cggcgtggtg gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg
    13561 gatgcgctcg gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt
    13621 cactgtcaca acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc
    13681 gcgcctggtg ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc
    13741 ggcccgttgg ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc
    13801 agcggcggcg ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt
    13861 tatgcgacta aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg
    13921 cgtaacttag gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa
    13981 gccgactgca ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc
    14041 ctgtggttgg catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa
    14101 tatccgatta ttctaa
//
