LOCUS       sequence-469118-       14286 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CinH Recombinase switch; plant T-DNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14286)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 14286)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC430
FEATURES             Location/Qualifiers
     source          1..14286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    21..45
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     promoter        542..887
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     830..850
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             1138..1935
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR
                     PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLL
                     SSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEH
                     QGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDI
                     ALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1195..1214)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1805..1824
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     terminator      1945..2193
                     /label=HSP terminator
                     /note="efficient transcription terminator from the 
                     Arabidopsis thaliana heat shock protein 18.2 gene (Nagaya 
                     et al., 2010)"
     CDS             2416..6366
                     /label=RUBY
     CDS             3916..3972
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             4807..4863
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     polyA_signal    6383..6557
                     /label=CaMV poly(A) signal
                     /note="cauliflower mosaic virus polyadenylation signal"
     misc_feature    6689..6713
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             7138..7932
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     rep_origin      9357..9945
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9846..9865
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10099..10116
                     /label=L4440
                     /note="L4440 vector, forward primer"
     misc_feature    10131..10271
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     complement(10197..10216)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     10357..10379
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     rep_origin      10615..10809
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(10875..11948)
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     CDS             complement(12377..13006)
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 taaacgctct tttctcttag gtttacccgc caatatatcc tgtcaaacac tgatagtttt
       61 tcgtacgacc ctcggtaccg atcggcgcgc cggagagatc gcgcacgcca atgctaccgt
      121 aggtcctgct tcaggaggtc aacatggtgg agcacgacac tctggtctac tccaaaaatg
      181 tcaaagatac agtctcagaa gatcaaaggg ctattgagac ttttcaacaa aggataattt
      241 cgggaaacct cctcggattc cattgcccag ctatctgtca cttcatcgaa aggacagtag
      301 aaaaggaagg tggctcctac aaatgccatc attgcgataa aggaaaggct atcattcaag
      361 atctctctgc cgacagtggt cccaaagatg gacccccacc cacgaggagc atcgtggaaa
      421 aagaagaggt tccaaccacg tctacaaagc aagtggattg atgtgataac atggtggagc
      481 acgacactct ggtctactcc aaaaatgtca aagatacagt ctcagaagat caaagggcta
      541 ttgagacttt tcaacaaagg ataatttcgg gaaacctcct cggattccat tgcccagcta
      601 tctgtcactt catcgaaagg acagtagaaa aggaaggtgg ctcctacaaa tgccatcatt
      661 gcgataaagg aaaggctatc attcaagatc tctctgccga cagtggtccc aaagatggac
      721 ccccacccac gaggagcatc gtggaaaaag aagaggttcc aaccacgtct acaaagcaag
      781 tggattgatg tgacatctcc actgacgtaa gggatgacgc acaatcccac tatccttcgc
      841 aagacccttc ctctatataa ggaagttcat ttcatttgga gaggacacgc tcgagtataa
      901 gagctcattt ttacaacaat taccaacaac aacaaacaac aaacaacatt acaattacat
      961 ttacaattat cgatacaatc tgagaacacg acgctgagct cggacggcgc gccggagcgt
     1021 aaattataaa ctttaaatat caaagttaca tgttatatat ggttaaaaat catttaaatg
     1081 ttacatagtt ttaagaactt ttatattgta actttagggt ataccccaaa gtaacgaatg
     1141 gggattgaac aagatggatt gcacgcaggt tctccggccg cttgggtgga gaggctattc
     1201 ggctatgact gggcacaaca gacaatcggc tgctctgatg ccgccgtgtt tcggctgtca
     1261 gcgcaggggc gcccggttct ttttgtcaag accgacctgt ccggtgccct gaatgaactc
     1321 caggacgagg cagcgcggct atcgtggctg gccacgacgg gcgttccttg cgcagctgtg
     1381 ctcgacgttg tcactgaagc gggaagggac tggctgctat tgggcgaagt gccggggcag
     1441 gatctcctgt catctcacct tgctcctgcc gagaaagtat ccatcatggc tgatgcaatg
     1501 cggcggctgc atacgcttga tccggctacc tgcccattcg accaccaagc gaaacatcgc
     1561 atcgagcgag cacgtactcg gatggaagcc ggtcttgtcg atcaggatga tctggacgaa
     1621 gaacatcagg ggctcgcgcc agccgaactg ttcgccaggc tcaaggcgcg catgcccgac
     1681 ggcgaggatc tcgtcgtgac acatggcgat gcctgcttgc cgaatatcat ggtggaaaat
     1741 ggccgctttt ctggattcat cgactgtggc cggctgggtg tggcggaccg ctatcaggac
     1801 atagcgttgg ctacccgtga tattgctgaa gaacttggcg gcgaatgggc tgaccgcttc
     1861 ctcgtgcttt acggtatcgc cgctcccgat tcgcagcgca tcgccttcta tcgccttctt
     1921 gacgagttct tctgagcggg cttatatgaa gatgaagatg aaatatttgg tgtgtcaaat
     1981 aaaaagcttg tgtgcttaag tttgtgtttt tttcttggct tgttgtgtta tgaatttgtg
     2041 gctttttcta atattaaatg aatgtaagat cacattataa tgaataaaca aatgtttcta
     2101 taatccattg tgaatgtttt gttggatctc ttctgcagca tataactact gtatgtgcta
     2161 tggtatggac tatggaatat gattaaagat aagcgctgag ctcccggggc gcgccggaga
     2221 gatcgcgcac gccaatgcta ccgtaggtcc tgcttcgtaa attataaact ttaaatatca
     2281 aagttacatg ttatatatgg ttaaaaatca tttaaatgtt acatagtttt aagaactttt
     2341 atattgtaac tttagggtat accccaaagt aacgcgctga gctctcccgg cgcgccggag
     2401 agatcgcgca cgccaatgga tcatgcgacc ctcgccatga tcctcgcgat ctggttcatc
     2461 agcttccact tcatcaagct gctgttctcc cagcagacca ccaagctgct tccgccagga
     2521 ccaaagccgc ttccgatcat cggcaacatc cttgaggtgg gcaagaagcc gcatcggtcc
     2581 ttcgccaacc tcgccaagat tcacggccca ctcatttccc tcagactcgg ctctgtgacc
     2641 accatcgttg tgtcctctgc cgacgtggcc aaagagatgt tcctcaagaa ggatcacccg
     2701 ctctccaacc gcacgatccc gaatagtgtt acagccggcg accaccacaa gctcaccatg
     2761 tcttggctcc cggtgtctcc gaagtggcgc aacttccgca agattaccgc cgtgcatctg
     2821 ctctccccac agagactcga tgcctgccag acattcaggc acgccaaggt gcagcagctc
     2881 tacgagtacg ttcaagagtg cgcccagaaa ggccaggccg tggatattgg caaggccgcc
     2941 tttacgacca gcctcaacct cctcagcaag ctgttcttca gcgtcgagct ggcgcaccac
     3001 aagtcccata ccagccaaga gttcaaagag ctgatctgga acatcatgga agatataggc
     3061 aagccgaact acgccgacta cttcccgatt ctcggctgcg ttgacccatc tggcattaga
     3121 agaaggctcg cctgctcctt cgacaagctg atcgccgtgt tccagggcat catctgcgag
     3181 agactcgccc cagattcctc caccacaact accaccacca ccgacgacgt gctcgatgtg
     3241 ctcctccagc tgttcaagca gaacgagctg acgatgggcg agatcaacca cctcctcgtg
     3301 gacatcttcg acgccggcac cgataccaca tcctccacat tcgagtgggt gatgaccgag
     3361 ctgatccgca atccagagat gatggaaaag gcccaagagg aaatcaagca ggtcctcggc
     3421 aaggacaagc agatccaaga gtccgacatc atcaacctgc cgtacctcca ggcgatcatc
     3481 aaagagacac tccgcctcca tccgccgacc gtgttcttgc tcccaagaaa ggccgacacc
     3541 gatgtcgagc tgtacggcta catcgtgccg aaggatgccc agatcctcgt gaacctctgg
     3601 gccattggca gggacccaaa cgcctggcag aacgccgata ttttcagccc agagcgcttc
     3661 atcggctgcg agatcgatgt taagggccgc gatttcggcc tccttccatt tggcgctggc
     3721 cgcagaattt gcccaggcat gaatctcgcc atcaggatgc tcaccctcat gctcgccaca
     3781 ctcctccagt tcttcaactg gaagctcgaa ggcgacatct ccccgaagga cctcgacatg
     3841 gacgagaagt tcggcattgc gctccaaaag accaagccgc tcaagctcat cccgattccg
     3901 cgctacggta gcggagctac caattttagc ctccttaagc aggctggtga tgtagaagag
     3961 aaccccgggc ctatgaagat gatgaacggc gaggacgcca acgaccagat gatcaaagag
     4021 tccttcttca tcacccacgg caacccgatc ctcaccgtcg aggatacaca tccgctcagg
     4081 ccgttcttcg agacatggcg cgagaagatt ttctccaaga agccgaaggc catcctcatc
     4141 atctccggcc actgggagac agtgaagcca accgtgaacg ccgtgcacat caacgacacc
     4201 atccacgact tcgacgacta cccagccgcc atgtaccagt tcaagtaccc agctccaggc
     4261 gagccagagc ttgcgagaaa ggtggaagag atcctcaaga agtccgggtt cgagacagcc
     4321 gagacagacc aaaagagggg ccttgatcac ggcgcctggg ttccactcat gctcatgtat
     4381 ccagaggcgg acatcccggt gtgccagctc tcagttcagc cacatctcga cggcacctac
     4441 cactacaatc tcggcagagc cctcgcgccg ctcaagaatg atggcgtgct cattattggc
     4501 tccggcagcg ccacacatcc actcgatgag acaccgcact acttcgatgg tgttgcccct
     4561 tgggccgctg ccttcgattc ttggcttagg aaggccctca tcaacggccg cttcgaggaa
     4621 gtgaacatct acgagagcaa ggccccgaac tggaagctcg cccatccatt tccagagcac
     4681 ttctacccgc tccacgttgt gctcggcgct gctggtgaaa agtggaaggc cgagctgatc
     4741 cactcctcct gggatcatgg cacactttgc cacggctcct acaagttcac ctccgccgga
     4801 tccggagcaa ccaactttag cctgctcaag caagcaggag atgttgagga aaatcctggc
     4861 cccatgaccg ccatcaagat gaacaccaac ggcgagggcg agacacagca catcctcatg
     4921 atcccgttca tggcgcaggg ccacctcagg ccatttctcg aactcgccat gttcctctac
     4981 aagcgctccc acgtgatcat caccctgctc acaactccgc tcaatgccgg cttcctcagg
     5041 cacctccttc accaccattc ctactcctcc agcggcatca ggatcgtcga gctgccattc
     5101 aactccacca accacggact cccaccgggc atcgagaaca ccgataagct cacactcccg
     5161 ctcgtggtgt ccctcttcca ttccaccatc agcctcgatc cgcacctccg cgattacatc
     5221 tccaggcatt tcagcccagc caggccacca ctctgcgtga tccatgatgt gttcctcggc
     5281 tgggttgacc aggtggccaa ggatgtgggc tctacaggcg tggtgttcac aacaggcggc
     5341 gcttatggca catccgccta cgtgtccatc tggaacgatc tcccgcacca gaactactcc
     5401 gacgaccaag agttcccgct gccaggcttc ccagagaacc ataagttccg caggtcccag
     5461 ctccatcggt tcctcagata tgccgacggc tccgacgatt ggtccaagta tttccagccg
     5521 cagctccgcc agtccatgaa gtcttttggc tggctctgca actccgtgga agagatcgag
     5581 acactcggct tctccatcct ccgcaactac accaagctgc cgatctgggg catcggccca
     5641 cttattgctt ccccagtgca gcactcctcc tccgacaaca attcaacagg cgccgagttc
     5701 gtgcagtggc tcagcctcaa agagccggac tccgtcctct acatctcctt cggctcccag
     5761 aacacgatca gcccgacgca gatgatggaa ctcgctgctg gccttgagtc ctccgagaag
     5821 ccattcctct gggtgatcag agccccgttc ggcttcgaca tcaacgaaga gatgcgccca
     5881 gagtggctgc cagagggctt tgaggaacgc atgaaggtga agaaacaggg caagctcgtg
     5941 tacaagctcg gcccgcagct tgagatcctc aaccatgaat ccatcggcgg ctttctcacc
     6001 cactgcggat ggaacagcat ccttgagtct cttcgcgagg gcgttccgat gcttggatgg
     6061 ccacttgctg ccgagcaggc ctacaacctc aagtacctcg aagatgagat gggcgtcgcg
     6121 gttgagcttg ctagaggcct cgaaggcgag atctccaaag agaaggtcaa gcgcatcgtc
     6181 gagatgatcc ttgagcgcaa cgagggctcc aaaggctggg agatgaagaa tcgcgccgtg
     6241 gaaatgggca aaaagctcaa ggacgccgtg aacgaggaaa aagagctgaa gggctcctcc
     6301 gtgaaggcga tcgacgattt cctcgacgcc gtcatgcagg ccaaacttga gccaagcctc
     6361 cagtgatagt gagcttctcg agtttctcca taataatgtg tgagtagttc ccagataagg
     6421 gaattagggt tcctataggg tttcgctcat gtgttgagca tataagaaac ccttagtatg
     6481 tatttgtatt tgtaaaatac ttctatcaat aaaatttcta attcctaaaa ccaaaatcca
     6541 gtactaaaat ccagatcccc cgaattacgc tgagctcagt gtttgatcgc cggcggtacc
     6601 gagtgtactt caagtcactc aattcggcgt taattcagta cattaaaaac gtccgcaatg
     6661 tgttattaag ttgtctaagc gtcaatttgt ttacaccaca atatatcctg ccaccagcca
     6721 gccaacagct ccccgaccgg cagctcggca caaaatcacc actcgataca ggcagcccat
     6781 cagtccggga cggcgtcagc gggagagccg ttgtaaggcg gcagactttg ctcatgttac
     6841 cgatgctatt cggaagaacg gcaactaagc tgccgggttt gaaacacgga tgatctcgcg
     6901 gagggtagca tgttgattgt aacgatgaca gagcgttgct gcctgtgatc accgcggttt
     6961 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     7021 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     7081 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     7141 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     7201 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     7261 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     7321 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     7381 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     7441 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     7501 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     7561 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     7621 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     7681 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     7741 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     7801 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     7861 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     7921 gaattgtttt agtacctaga atgcatgacc aaaatccctt aacgctgatg aatcccctaa
     7981 tgattttggt aaaaatcatt aagttaaggt ggatacacat cttgtcatat gatcaaatgg
     8041 tttcgcgaaa aatcaataat cagacaacaa gatgtgcgaa ctcgatattt tacacgactc
     8101 tctttaccaa ttctgccccg aattacactt aaaacgactc aacagcttaa cgttggcttg
     8161 ccacgcatta cttgactgta aaactctcac tcttaccgaa cttggccgta acctgccaac
     8221 caaagcgaga acaaaacata acatcaaacg aatcgaccga ttgttaggta atcgtcacct
     8281 ccacaaagag cgactcgctg tataccgttg gcatgctagc tttatctgtt cgggcaatac
     8341 gatgcccatt gtacttgttg actggtctga tattcgtgag caaaaacgac ttatggtatt
     8401 gcgagcttca gtcgcactac acggtcgttc tgttactctt tatgagaaag cgttcccgct
     8461 ttcagagcaa tgttcaaaga aagctcatga ccaatttcta gccgaccttg cgagcattct
     8521 accgagtaac accacaccgc tcattgtcag tgatgctggc tttaaagtgc catggtataa
     8581 atccgttgag aagctgggtt ggtactggtt aagtcgagta agaggaaaag tacaatatgc
     8641 agacctagga gcggaaaact ggaaacctat cagcaactta catgatatgt catctagtca
     8701 ctcaaagact ttaggctata agaggctgac taaaagcaat ccaatctcat gccaaattct
     8761 attgtataaa tctcgctcta aaggccgaaa aaatcagcgc tcgacacgga ctcattgtca
     8821 ccacccgtca cctaaaatct actcagcgtc ggcaaaggag ccatgggttc tagcaactaa
     8881 cttacctgtt gaaattcgaa cacccaaaca acttgttaat atctattcga agcgaatgca
     8941 gattgaagaa accttccgag acttgaaaag tcctgcctac ggactaggcc tacgccatag
     9001 ccgaacgagc agctcagagc gttttgatat catgctgcta atcgccctga tgcttcaact
     9061 aacatgttgg cttgcgggcg ttcatgctca gaaacaaggt tgggacaagc acttccaggc
     9121 taacacagtc agaaatcgaa acgtactctc aacagttcgc ttaggcatgg aagttttgcg
     9181 gcattctggc tacacaataa caagggaaga cttactcgtg gctgcaaccc tactagctca
     9241 aaatttattc acacatggtt acgctttggg gaaattatga ggggatctct cagcccttaa
     9301 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     9361 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     9421 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     9481 agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag
     9541 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     9601 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     9661 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     9721 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     9781 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     9841 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     9901 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     9961 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
    10021 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
    10081 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagaggg cctgatgcgg
    10141 tattttctcc ttacgcatct gtgcggtatt tcacaccgca tatggtgcac tctcagtaca
    10201 atctgctctg atgccgcata gttaagccag tatacactcc gctatcgcta cgtgactggg
    10261 tcatggctgc gccccgacac ccgccaacac ccgctgacgc gccctgacgg gcttgtctgc
    10321 tcccggcatc cgcttacaga caagctgtga ccgtttccgg gagctgcatg tgtcagaggt
    10381 tttcaccgtc atcaccgaaa cgcgcgaggc agggtgcctt gatgtgggcg ccggcggtcg
    10441 agtggcgacg gcgcggcttg tccgcgccct ggtagattgc ctggccgtag gccagccatt
    10501 tttgagcggc cagcggccgc gataggccga cgcgaagcgg cggggcgtag ggagcgcagc
    10561 gaccgaaggg taggcgcttt ttgcagctct tgggctgtgc gctggccaga cagttatgca
    10621 caggccaggc gggttttaag agttttaata agttttaaag agttttaggc ggaaaaatcg
    10681 ccttttttct cttttatatc agtcacttac atgtgtgacc ggttcccaat gtacggcttt
    10741 gggttcccaa tgtacgggtt ccggttccca atgtacggct ttgggttccc aatgtacgtg
    10801 ctatccacag gaaagaggcc ttttcgacct ttttcccctg ctagggcaat ttgccctagc
    10861 atctgctccg tacattagga accggcggat gcttcgccct cgatcaggtt gcggtagcgc
    10921 atgactagga tcgggccagc ctgccccgcc tcctccttca aatcgtactc cggcaggtca
    10981 tttgacccga tcagcttgcg cacggtgaaa cagaacttct tgaactctcc ggcgctgcca
    11041 ctgcgttcgt agatcgtctt gaacaaccat ctggcttctg ccttgcctgc ggcgcggcgt
    11101 gccaggcggt agagaaaacg gccgatgccg ggatcgatca aaaagtaatc ggggtgaacc
    11161 gtcagcacgt ccgggttctt gccttctgtg atctcgcggt acatccaatc agctagctcg
    11221 atctcgatgt actccggccg cccggtttcg ctctttacga tcttgtagcg gctaatcaag
    11281 gcttcaccct cggataccgt caccaggcgg ccgttcttgg ccttcttcgt acgctgcatg
    11341 gcaacgtgcg tggtgtttaa ccgaatgcag gtttctacca ggtcgtcttt ctgctttccg
    11401 ccatcggctc gccggcagaa cttgagtacg tccgcaacgt gtggacggaa cacgcggccg
    11461 ggcttgtctc ccttcccttc ccggtatcgg ttcatggatt cggttagatg ggaaaccgcc
    11521 atcagtacca ggtcgtaatc ccacacactg gccatgccgg ccggccctgc ggaaacctct
    11581 acgtgcccgt ctggaagctc gtagcggatc acctcgccag ctcgtcggtc acgcttcgac
    11641 agacggaaaa cggccacgtc catgatgctg cgactatcgc gggtgcccac gtcatagagc
    11701 atcggaacga aaaaatctgg ttgctcgtcg cccttgggcg gcttcctaat cgacggcgca
    11761 ccggctgccg gcggttgccg ggattctttg cggattcgat cagcggccgc ttgccacgat
    11821 tcaccggggc gtgcttctgc ctcgatgcgt tgccgctggg cggcctgcgc ggccttcaac
    11881 ttctccacca ggtcatcacc cagcgccgcg ccgatttgta ccgggccgga tggtttgcga
    11941 ccgctcacgc cgattcctcg ggcttggggg ttccagtgcc attgcagggc cggcaggcaa
    12001 cccagccgct tacgcctggc caaccgcccg ttcctccaca catggggcat tccacggcgt
    12061 cggtgcctgg ttgttcttga ttttccatgc cgcctccttt agccgctaaa attcatctac
    12121 tcatttattc atttgctcat ttactctggt agctgcgcga tgtattcaga tagcagctcg
    12181 gtaatggtct tgccttggcg taccgcgtac atcttcagct tggtgtgatc ctccgccggc
    12241 aactgaaagt tgacccgctt catggctggc gtgtctgcca ggctggccaa cgttgcagcc
    12301 ttgctgctgc gtgcgctcgg acggccggca cttagcgtgt ttgtgctttt gctcattttc
    12361 tctttacctc attaactcaa atgagttttg atttaatttc agcggccagc gcctggacct
    12421 cgcgggcagc gtcgccctcg ggttctgatt caagaacggt tgtgccggcg gcggcagtgc
    12481 ctgggtagct cacgcgctgc gtgatacggg actcaagaat gggcagctcg tacccggcca
    12541 gcgcctcggc aacctcaccg ccgatgcgcg tgcctttgat cgcccgcgac acgacaaagg
    12601 ccgcttgtag ccttccatcc gtgacctcaa tgcgctgctt aaccagctcc accaggtcgg
    12661 cggtggccca tatgtcgtaa gggcttggct gcaccggaat cagcacgaag tcggctgcct
    12721 tgatcgcgga cacagccaag tccgccgcct ggggcgctcc gtcgatcact acgaagtcgc
    12781 gccggccgat ggccttcacg tcgcggtcaa tcgtcgggcg gtcgatgccg acaacggtta
    12841 gcggttgatc ttcccgcacg gccgcccaat cgcgggcact gccctgggga tcggaatcga
    12901 ctaacagaac atcggccccg gcgagttgca gggcgcgggc tagatgggtt gcgatggtcg
    12961 tcttgcctga cccgcctttc tggttaagta cagcgataac cttcatgcgt tccccttgcg
    13021 tatttgttta tttactcatc gcatcatata cgcagcgacc gcatgacgca agctgtttta
    13081 ctcaaataca catcaccttt ttagacggcg gcgctcggtt tcttcagcgg ccaagctggc
    13141 cggccaggcc gccagcttgg catcagacaa accggccagg atttcatgca gccgcacggt
    13201 tgagccgtgc gcgggcggct cgaacacgta cccggccgcg atcatctccg cctcgatctc
    13261 ttcggtaatg aaaaacggtt cgtcctggcc gtcctggtgc ggtttcatgc ttgttcctct
    13321 tggcgttcat tctcggcggc cgccagggcg tcggcctcgg tcaatgcgtc ctcacggaag
    13381 gcaccgcgcc gcctggcctc ggtgggcgtc acttcctcgc tgcgctcaag tgcgcggtac
    13441 agggtcgagc gatgcacgcc aagcagtgca gccgcctctt tcacggtgcg gccttcctgg
    13501 tcgatcagct cgcgggcgtg cgcgatctgt gccggggtga gggtagggcg ggggccaaac
    13561 ttcacgcctc gggccttggc ggcctcgcgc ccgctccggg tgcggtcgat gattagggaa
    13621 cgctcgaact cggcaatgcc ggcgaacacg gtcaacacca tgcggccggc cggcgtggtg
    13681 gtgtcggccc acggctctgc caggctacgc aggcccgcgc cggcctcctg gatgcgctcg
    13741 gcaatgtcca gtaggtcgcg ggtgctgcgg gccaggcggt ctagcctggt cactgtcaca
    13801 acgtcgccag ggcgtaggtg gtcaagcatc ctggccagct ccgggcggtc gcgcctggtg
    13861 ccggtgatct tctcggaaaa cagcttggtg cagccggccg cgtgcagttc ggcccgttgg
    13921 ttggtcaagt cctggtcgtc ggtgctgacg cgggcatagc ccagcaggcc agcggcggcg
    13981 ctcttgttca tggcgtaatg tctccggttc tagtcgcaag tattctactt tatgcgacta
    14041 aaacacgcga caagaaaacg ccaggaaaag ggcagggcgg cagcctgtcg cgtaacttag
    14101 gacttgtgcg acatgtcgtt ttcagaagac ggctgcactg aacgtcagaa gccgactgca
    14161 ctatagcagc ggaggggttg gatcaaagta ctttgatccc gaggggaacc ctgtggttgg
    14221 catgcacata caaatggacg aacggataaa ccttttcacg cccttttaaa tatccgatta
    14281 ttctaa
//
