LOCUS       sequence-469119-       11890 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRV2 expressing Cre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11890)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 11890)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC055
FEATURES             Location/Qualifiers
     source          1..11890
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        554..899
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     842..862
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             3947..3964
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      3999..4251
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4273..4297
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5598..6227
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6656..7729
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      7795..7989
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8225..8247)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8333..8473
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8388..8407
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8488..8505)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8659..9247)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8739..8758)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10672..11466)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      181 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      241 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      301 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      361 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      421 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      481 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      541 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      601 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      661 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      721 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
      901 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
      961 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1021 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1081 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1141 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1201 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1261 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1321 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1381 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1441 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1501 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1561 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1621 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1681 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1741 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1801 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1861 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     1921 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     1981 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2041 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2101 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2161 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2221 gtagcatttg agtttcggag catcttgttc tggggtttca cactatcttt agagaaagtg
     2281 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2341 agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact
     2401 gtatcactta cccgagttaa cgagtctaga aaccaaatga gcaacttgtt gacggtccat
     2461 caaaatcttc cggcgttgcc cgtcgatgcc acatccgatg aagtcagaaa gaacctcatg
     2521 gatatgtttc gggatcggca agcatttagc gaacacactt ggaagatgct cctgtccgtc
     2581 tgtaggagct gggctgcatg gtgcaaactc aacaatagaa agtggtttcc cgccgaaccc
     2641 gaggatgtgc gcgactacct cttgtacctg caagcgagag gattggcggt caagacaata
     2701 cagcagcatc tgggacaact caatatgctt catcgccggt ctggcttgcc tagaccaagc
     2761 gactctaatg cagtttcgtt ggtgatgcgg cgcattcgca aagagaacgt tgatgctggg
     2821 gaaagagcca aacaggcctt ggcgtttgaa cgcactgact tcgaccaagt caggagcctg
     2881 atggagaaca gcgaccgctg ccaagacatc aggaacctgg cattcctcgg gatagcgtat
     2941 aacacgcttc tccgcatagc ggaaattgct cggattcggg tcaaggatat atcaagaaca
     3001 gacggcgggc ggatgttgat tcacatcgga cgcactaaaa cgttggtgtc aactgccggg
     3061 gtggagaagg ctctctcttt gggtgtcact aaacttgtgg agcggtggat ctcggtcagc
     3121 ggcgtggctg atgacccgaa taactatctt ttttgccggg tcagaaaaaa tggtgtggcg
     3181 gccccttcgg cgacctcaca actctcgact cgggctctgg aaggcatatt tgaagcgacg
     3241 catcggctga tttatggtgc caaagatgat agcgggcaaa ggtacctcgc gtggtctggg
     3301 cactctgcga gagtgggggc agcacgcgat atggcgcgcg ctggtgtctc aatccccgaa
     3361 attatgcaag ccgggggctg gaccaatgtt aatatcgtga tgaattacat taggaacctc
     3421 gatagcgaga ctggggccat ggtgcggctt cttgaggatg gagattagct ttgagaccac
     3481 gagtgccggg catgtcccga agacattaaa ctacggttct ttaagtagat ccgtgtctga
     3541 agttttaggt tcaatttaaa cctacgagat tgacattctc gactgatctt gattgatcgg
     3601 taagtctttt gtaatttaat tttctttttg attttatttt aaattgttat ctgtttctgt
     3661 gtatagactg tttgagatcg gcgtttggcc gactcattgt cttaccatag gggaacggac
     3721 tttgtttgtg ttgttatttt atttgtattt tattaaaatt ctcaacgatc tgaaaaagcc
     3781 tcgcggctaa gagattgttg gggggtgagt aagtactttt aaagtgatga tggttacaaa
     3841 ggcaaaaggg gtaaaacccc tcgcctacgt aagcgttatt acgcccgtct gtacttatat
     3901 cagtacactg acgagtccct aaaggacgaa acgggagaac gctagccacc accaccacca
     3961 ccacgtgtga attacaggtg accagctcga atttccccga tcgttcaaac atttggcaat
     4021 aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata taatttctgt
     4081 tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt atgagatggg
     4141 tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac aaaatatagc
     4201 gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat cgggaattaa
     4261 actatcagtg tttgacagga tatattggcg ggtaaaccta agagaaaaga gcgtttatta
     4321 gaataatcgg atatttaaaa gggcgtgaaa aggtttatcc gttcgtccat ttgtatgtgc
     4381 atgccaacca cagggttccc ctcgggatca aagtactttg atccaacccc tccgctgcta
     4441 tagtgcagtc ggcttctgac gttcagtgca gccgtcttct gaaaacgaca tgtcgcacaa
     4501 gtcctaagtt acgcgacagg ctgccgccct gcccttttcc tggcgttttc ttgtcgcgtg
     4561 ttttagtcgc ataaagtaga atacttgcga ctagaaccgg agacattacg ccatgaacaa
     4621 gagcgccgcc gctggcctgc tgggctatgc ccgcgtcagc accgacgacc aggacttgac
     4681 caaccaacgg gccgaactgc acgcggccgg ctgcaccaag ctgttttccg agaagatcac
     4741 cggcaccagg cgcgaccgcc cggagctggc caggatgctt gaccacctac gccctggcga
     4801 cgttgtgaca gtgaccaggc tagaccgcct ggcccgcagc acccgcgacc tactggacat
     4861 tgccgagcgc atccaggagg ccggcgcggg cctgcgtagc ctggcagagc cgtgggccga
     4921 caccaccacg ccggccggcc gcatggtgtt gaccgtgttc gccggcattg ccgagttcga
     4981 gcgttcccta atcatcgacc gcacccggag cgggcgcgag gccgccaagg cccgaggcgt
     5041 gaagtttggc ccccgcccta ccctcacccc ggcacagatc gcgcacgccc gcgagctgat
     5101 cgaccaggaa ggccgcaccg tgaaagaggc ggctgcactg cttggcgtgc atcgctcgac
     5161 cctgtaccgc gcacttgagc gcagcgagga agtgacgccc accgaggcca ggcggcgcgg
     5221 tgccttccgt gaggacgcat tgaccgaggc cgacgccctg gcggccgccg agaatgaacg
     5281 ccaagaggaa caagcatgaa accgcaccag gacggccagg acgaaccgtt tttcattacc
     5341 gaagagatcg aggcggagat gatcgcggcc gggtacgtgt tcgagccgcc cgcgcacgtc
     5401 tcaaccgtgc ggctgcatga aatcctggcc ggtttgtctg atgccaagct ggcggcctgg
     5461 ccggccagct tggccgctga agaaaccgag cgccgccgtc taaaaaggtg atgtgtattt
     5521 gagtaaaaca gcttgcgtca tgcggtcgct gcgtatatga tgcgatgagt aaataaacaa
     5581 atacgcaagg ggaacgcatg aaggttatcg ctgtacttaa ccagaaaggc gggtcaggca
     5641 agacgaccat cgcaacccat ctagcccgcg ccctgcaact cgccggggcc gatgttctgt
     5701 tagtcgattc cgatccccag ggcagtgccc gcgattgggc ggccgtgcgg gaagatcaac
     5761 cgctaaccgt tgtcggcatc gaccgcccga cgattgaccg cgacgtgaag gccatcggcc
     5821 ggcgcgactt cgtagtgatc gacggagcgc cccaggcggc ggacttggct gtgtccgcga
     5881 tcaaggcagc cgacttcgtg ctgattccgg tgcagccaag cccttacgac atatgggcca
     5941 ccgccgacct ggtggagctg gttaagcagc gcattgaggt cacggatgga aggctacaag
     6001 cggcctttgt cgtgtcgcgg gcgatcaaag gcacgcgcat cggcggtgag gttgccgagg
     6061 cgctggccgg gtacgagctg cccattcttg agtcccgtat cacgcagcgc gtgagctacc
     6121 caggcactgc cgccgccggc acaaccgttc ttgaatcaga acccgagggc gacgctgccc
     6181 gcgaggtcca ggcgctggcc gctgaaatta aatcaaaact catttgagtt aatgaggtaa
     6241 agagaaaatg agcaaaagca caaacacgct aagtgccggc cgtccgagcg cacgcagcag
     6301 caaggctgca acgttggcca gcctggcaga cacgccagcc atgaagcggg tcaactttca
     6361 gttgccggcg gaggatcaca ccaagctgaa gatgtacgcg gtacgccaag gcaagaccat
     6421 taccgagctg ctatctgaat acatcgcgca gctaccagag taaatgagca aatgaataaa
     6481 tgagtagatg aattttagcg gctaaaggag gcggcatgga aaatcaagaa caaccaggca
     6541 ccgacgccgt ggaatgcccc atgtgtggag gaacgggcgg ttggccaggc gtaagcggct
     6601 gggttgcctg ccggccctgc aatggcactg gaacccccaa gcccgaggaa tcggcgtgag
     6661 cggtcgcaaa ccatccggcc cggtacaaat cggcgcggcg ctgggtgatg acctggtgga
     6721 gaagttgaag gccgcgcagg ccgcccagcg gcaacgcatc gaggcagaag cacgccccgg
     6781 tgaatcgtgg caagcggccg ctgatcgaat ccgcaaagaa tcccggcaac cgccggcagc
     6841 cggtgcgccg tcgattagga agccgcccaa gggcgacgag caaccagatt ttttcgttcc
     6901 gatgctctat gacgtgggca cccgcgatag tcgcagcatc atggacgtgg ccgttttccg
     6961 tctgtcgaag cgtgaccgac gagctggcga ggtgatccgc tacgagcttc cagacgggca
     7021 cgtagaggtt tccgcagggc cggccggcat ggccagtgtg tgggattacg acctggtact
     7081 gatggcggtt tcccatctaa ccgaatccat gaaccgatac cgggaaggga agggagacaa
     7141 gcccggccgc gtgttccgtc cacacgttgc ggacgtactc aagttctgcc ggcgagccga
     7201 tggcggaaag cagaaagacg acctggtaga aacctgcatt cggttaaaca ccacgcacgt
     7261 tgccatgcag cgtacgaaga aggccaagaa cggccgcctg gtgacggtat ccgagggtga
     7321 agccttgatt agccgctaca agatcgtaaa gagcgaaacc gggcggccgg agtacatcga
     7381 gatcgagcta gctgattgga tgtaccgcga gatcacagaa ggcaagaacc cggacgtgct
     7441 gacggttcac cccgattact ttttgatcga tcccggcatc ggccgttttc tctaccgcct
     7501 ggcacgccgc gccgcaggca aggcagaagc cagatggttg ttcaagacga tctacgaacg
     7561 cagtggcagc gccggagagt tcaagaagtt ctgtttcacc gtgcgcaagc tgatcgggtc
     7621 aaatgacctg ccggagtacg atttgaagga ggaggcgggg caggctggcc cgatcctagt
     7681 catgcgctac cgcaacctga tcgagggcga agcatccgcc ggttcctaat gtacggagca
     7741 gatgctaggg caaattgccc tagcagggga aaaaggtcga aaaggtctct ttcctgtgga
     7801 tagcacgtac attgggaacc caaagccgta cattgggaac cggaacccgt acattgggaa
     7861 cccaaagccg tacattggga accggtcaca catgtaagtg actgatataa aagagaaaaa
     7921 aggcgatttt tccgcctaaa actctttaaa acttattaaa actcttaaaa cccgcctggc
     7981 ctgtgcataa ctgtctggcc agcgcacagc cgaagagctg caaaaagcgc ctacccttcg
     8041 gtcgctgcgc tccctacgcc ccgccgcttc gcgtcggcct atcgcggccg ctggccgctc
     8101 aaaaatggct ggcctacggc caggcaatct accagggcgc ggacaagccg cgccgtcgcc
     8161 actcgaccgc cggcgcccac atcaaggcac cctgcctcgc gcgtttcggt gatgacggtg
     8221 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
     8281 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
     8341 tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg catcagagca
     8401 gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     8461 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg
     8521 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg
     8581 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa
     8641 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg
     8701 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc
     8761 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc
     8821 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc
     8881 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg
     8941 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc
     9001 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga
     9061 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc
     9121 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac
     9181 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg
     9241 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc
     9301 acgttaaggg ctgatgaatc ccctaatgat ttttatcaaa atcattaagt taaggtagat
     9361 acacatcttg tcatatgatc aaatggtttc gccaaaaatc aataatcaga caacaaaatg
     9421 tgcgaactcg atattttaca cgactctctt taccaattct gccccgaatt acacttaaaa
     9481 cgactcaaca gcttaacgtt ggcttgccac gccttacttg actgtaaaac tctcactctt
     9541 accgaacttg gccgtaacct gccaaccaaa gcgagaacaa aacataacat caaacgaatc
     9601 gaccgattgt taggtaatcg tcacctccac aaagagcgac tcgctgtata ccgttggcat
     9661 gctagcttta tctgttcggg caatacgatg cccattgtac ttgttgactg gtctgatatc
     9721 cgtgagcaaa aacggcttat ggtattgcga gcttcagtcg cactacacgg tcgttctgtt
     9781 actctttatg agaaagcgtt cccgctttca gagcaatgtt caaagaaagc tcatgaccaa
     9841 tttctagccg accttgcgag cattctaccg agtaacacca caccgctcat tgtcagtgat
     9901 gctggcttta aagtgccatg gtataaatcc gttgagaagc tgggttggta ctggttaagt
     9961 cgagtaagag gaaaagtaca atatgcagac ctaggagcgg aaaactggaa acctatcagc
    10021 aacttacatg atatgtcatc tagtcactca aagactttag gctataagag gctgactaaa
    10081 agcaatccaa tctcatgcca aattctattg tataaatctc gctctaaagg ccgaaaaaat
    10141 cagcgctcga cacggactca ttgtcaccac ccgtcaccta aaatctactc agcgtcggca
    10201 aaggagccat ggattctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt
    10261 gttaatatct attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct
    10321 gcctacggac taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg
    10381 ctgctaatcg ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa
    10441 caaggttggg acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca
    10501 gttcgcttag gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactca
    10561 ctcgtggctg caaccctgct tactcaaaat ctattcacac atggttacgt tttggggaaa
    10621 ttatgagggg atctctcagc gttaagggat tttggtcatg cattctaggt actaaaacaa
    10681 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    10741 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    10801 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    10861 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    10921 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    10981 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    11041 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    11101 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    11161 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    11221 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    11281 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    11341 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    11401 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    11461 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    11521 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    11581 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    11641 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    11701 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    11761 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11821 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11881 ggctggctgg
//
