LOCUS       sequence-469144-       11830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRV2 expressing FLPe.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11830)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 11830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC060
FEATURES             Location/Qualifiers
     source          1..11830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        554..899
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     842..862
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             2434..3708
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=FLP
                     /note="FLP is a site-specific recombinase from 
                     Saccharomyces cerevisiae. Recombination occurs at FRT 
                     sequences."
                     /translation="MSQFDILCKTPPKVLVRQFVERFERPSGEKIASCAAELTYLCWMI
                     THNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEFTI
                     IPYNGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEKILN
                     SFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCLVTET
                     KTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLKDNLVR
                     SYNKALKKNAPYPIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKRASAVAR
                     TTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKGSAEGSIR
                     YPAWNGIISQEVLDYLSSYINRRIG"
     CDS             4178..4195
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4230..4482
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4504..4528
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5829..6458
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             6887..7960
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8026..8220
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8456..8478)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8564..8704
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8619..8638
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8719..8736)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8890..9478)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8970..8989)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10612..11406)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      181 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      241 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      301 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      361 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      421 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      481 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      541 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      601 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      661 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      721 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
      901 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
      961 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1021 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1081 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1141 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1201 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1261 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1321 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1381 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1441 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1501 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1561 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1621 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1681 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1741 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1801 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1861 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     1921 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     1981 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2041 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2101 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2161 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2221 gtagcatttg agtttcggag catcttgttc tggggtttca cactatcttt agagaaagtg
     2281 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2341 agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact
     2401 gtatcactta cccgagttaa cgagtctaga aacatgagcc aatttgatat attatgtaaa
     2461 acaccaccta aggtcctggt tcgtcagttt gtggaaaggt ttgaaagacc ttcaggggaa
     2521 aaaatagcat catgtgctgc tgaactaacc tatttatgtt ggatgattac tcataacgga
     2581 acagcaatca agagagccac attcatgagc tataatacta tcataagcaa ttcgctgagt
     2641 ttcgatattg tcaacaaatc actccagttt aaatacaaga cgcaaaaagc aacaattctg
     2701 gaagcctcat taaagaaatt aattcctgct tgggaattta caattattcc ttacaatgga
     2761 caaaaacatc aatctgatat cactgatatt gtaagtagtt tgcaattaca gttcgaatca
     2821 tcggaagaag cagataaggg aaatagccac agtaaaaaaa tgcttaaagc acttctaagt
     2881 gagggtgaaa gcatctggga gatcactgag aaaatactaa attcgtttga gtatacctcg
     2941 agatttacaa aaacaaaaac tttataccaa ttcctcttcc tagctacttt catcaattgt
     3001 ggaagattca gcgatattaa gaacgttgat ccgaaatcat ttaaattagt ccaaaataag
     3061 tatctgggag taataatcca gtgtttagtg acagagacaa agacaagcgt tagtaggcac
     3121 atatacttct ttagcgcaag gggtaggatc gatccacttg tatatttgga tgaatttttg
     3181 aggaactctg aaccagtcct aaaacgagta aataggaccg gcaattcttc aagcaacaaa
     3241 caggaatacc aattattaaa agataactta gtcagatcgt acaacaaggc tttgaagaaa
     3301 aatgcgcctt atccaatctt tgctataaag aatggcccaa aatctcacat tggaaggcat
     3361 ttgatgacct catttctgtc aatgaagggc ctaacggagt tgactaatgt tgtgggaaat
     3421 tggagcgata agcgtgcttc tgccgtggcc aggacaacgt atactcatca gataacagca
     3481 atacctgatc actacttcgc actagtttct cggtactatg catatgatcc aatatcaaag
     3541 gaaatgatag cattgaagga tgagactaat ccaattgagg agtggcagca tatagaacag
     3601 ctaaagggta gtgctgaagg aagcatacga taccccgcat ggaatgggat aatatcacag
     3661 gaggtactag actacctttc atcctacata aatagacgca taggatgagc ttagtgccgg
     3721 gcatgtcccg aagacattaa actacggttc tttaagtaga tccgtgtctg aagttttagg
     3781 ttcaatttaa acctacgaga ttgacattct cgactgatct tgattgatcg gtaagtcttt
     3841 tgtaatttaa ttttcttttt gattttattt taaattgtta tctgtttctg tgtatagact
     3901 gtttgagatc ggcgtttggc cgactcattg tcttaccata ggggaacgga ctttgtttgt
     3961 gttgttattt tatttgtatt ttattaaaat tctcaacgat ctgaaaaagc ctcgcggcta
     4021 agagattgtt ggggggtgag taagtacttt taaagtgatg atggttacaa aggcaaaagg
     4081 ggtaaaaccc ctcgcctacg taagcgttat tacgcccgtc tgtacttata tcagtacact
     4141 gacgagtccc taaaggacga aacgggagaa cgctagccac caccaccacc accacgtgtg
     4201 aattacaggt gaccagctcg aatttccccg atcgttcaaa catttggcaa taaagtttct
     4261 taagattgaa tcctgttgcc ggtcttgcga tgattatcat ataatttctg ttgaattacg
     4321 ttaagcatgt aataattaac atgtaatgca tgacgttatt tatgagatgg gtttttatga
     4381 ttagagtccc gcaattatac atttaatacg cgatagaaaa caaaatatag cgcgcaaact
     4441 aggataaatt atcgcgcgcg gtgtcatcta tgttactaga tcgggaatta aactatcagt
     4501 gtttgacagg atatattggc gggtaaacct aagagaaaag agcgtttatt agaataatcg
     4561 gatatttaaa agggcgtgaa aaggtttatc cgttcgtcca tttgtatgtg catgccaacc
     4621 acagggttcc cctcgggatc aaagtacttt gatccaaccc ctccgctgct atagtgcagt
     4681 cggcttctga cgttcagtgc agccgtcttc tgaaaacgac atgtcgcaca agtcctaagt
     4741 tacgcgacag gctgccgccc tgcccttttc ctggcgtttt cttgtcgcgt gttttagtcg
     4801 cataaagtag aatacttgcg actagaaccg gagacattac gccatgaaca agagcgccgc
     4861 cgctggcctg ctgggctatg cccgcgtcag caccgacgac caggacttga ccaaccaacg
     4921 ggccgaactg cacgcggccg gctgcaccaa gctgttttcc gagaagatca ccggcaccag
     4981 gcgcgaccgc ccggagctgg ccaggatgct tgaccaccta cgccctggcg acgttgtgac
     5041 agtgaccagg ctagaccgcc tggcccgcag cacccgcgac ctactggaca ttgccgagcg
     5101 catccaggag gccggcgcgg gcctgcgtag cctggcagag ccgtgggccg acaccaccac
     5161 gccggccggc cgcatggtgt tgaccgtgtt cgccggcatt gccgagttcg agcgttccct
     5221 aatcatcgac cgcacccgga gcgggcgcga ggccgccaag gcccgaggcg tgaagtttgg
     5281 cccccgccct accctcaccc cggcacagat cgcgcacgcc cgcgagctga tcgaccagga
     5341 aggccgcacc gtgaaagagg cggctgcact gcttggcgtg catcgctcga ccctgtaccg
     5401 cgcacttgag cgcagcgagg aagtgacgcc caccgaggcc aggcggcgcg gtgccttccg
     5461 tgaggacgca ttgaccgagg ccgacgccct ggcggccgcc gagaatgaac gccaagagga
     5521 acaagcatga aaccgcacca ggacggccag gacgaaccgt ttttcattac cgaagagatc
     5581 gaggcggaga tgatcgcggc cgggtacgtg ttcgagccgc ccgcgcacgt ctcaaccgtg
     5641 cggctgcatg aaatcctggc cggtttgtct gatgccaagc tggcggcctg gccggccagc
     5701 ttggccgctg aagaaaccga gcgccgccgt ctaaaaaggt gatgtgtatt tgagtaaaac
     5761 agcttgcgtc atgcggtcgc tgcgtatatg atgcgatgag taaataaaca aatacgcaag
     5821 gggaacgcat gaaggttatc gctgtactta accagaaagg cgggtcaggc aagacgacca
     5881 tcgcaaccca tctagcccgc gccctgcaac tcgccggggc cgatgttctg ttagtcgatt
     5941 ccgatcccca gggcagtgcc cgcgattggg cggccgtgcg ggaagatcaa ccgctaaccg
     6001 ttgtcggcat cgaccgcccg acgattgacc gcgacgtgaa ggccatcggc cggcgcgact
     6061 tcgtagtgat cgacggagcg ccccaggcgg cggacttggc tgtgtccgcg atcaaggcag
     6121 ccgacttcgt gctgattccg gtgcagccaa gcccttacga catatgggcc accgccgacc
     6181 tggtggagct ggttaagcag cgcattgagg tcacggatgg aaggctacaa gcggcctttg
     6241 tcgtgtcgcg ggcgatcaaa ggcacgcgca tcggcggtga ggttgccgag gcgctggccg
     6301 ggtacgagct gcccattctt gagtcccgta tcacgcagcg cgtgagctac ccaggcactg
     6361 ccgccgccgg cacaaccgtt cttgaatcag aacccgaggg cgacgctgcc cgcgaggtcc
     6421 aggcgctggc cgctgaaatt aaatcaaaac tcatttgagt taatgaggta aagagaaaat
     6481 gagcaaaagc acaaacacgc taagtgccgg ccgtccgagc gcacgcagca gcaaggctgc
     6541 aacgttggcc agcctggcag acacgccagc catgaagcgg gtcaactttc agttgccggc
     6601 ggaggatcac accaagctga agatgtacgc ggtacgccaa ggcaagacca ttaccgagct
     6661 gctatctgaa tacatcgcgc agctaccaga gtaaatgagc aaatgaataa atgagtagat
     6721 gaattttagc ggctaaagga ggcggcatgg aaaatcaaga acaaccaggc accgacgccg
     6781 tggaatgccc catgtgtgga ggaacgggcg gttggccagg cgtaagcggc tgggttgcct
     6841 gccggccctg caatggcact ggaaccccca agcccgagga atcggcgtga gcggtcgcaa
     6901 accatccggc ccggtacaaa tcggcgcggc gctgggtgat gacctggtgg agaagttgaa
     6961 ggccgcgcag gccgcccagc ggcaacgcat cgaggcagaa gcacgccccg gtgaatcgtg
     7021 gcaagcggcc gctgatcgaa tccgcaaaga atcccggcaa ccgccggcag ccggtgcgcc
     7081 gtcgattagg aagccgccca agggcgacga gcaaccagat tttttcgttc cgatgctcta
     7141 tgacgtgggc acccgcgata gtcgcagcat catggacgtg gccgttttcc gtctgtcgaa
     7201 gcgtgaccga cgagctggcg aggtgatccg ctacgagctt ccagacgggc acgtagaggt
     7261 ttccgcaggg ccggccggca tggccagtgt gtgggattac gacctggtac tgatggcggt
     7321 ttcccatcta accgaatcca tgaaccgata ccgggaaggg aagggagaca agcccggccg
     7381 cgtgttccgt ccacacgttg cggacgtact caagttctgc cggcgagccg atggcggaaa
     7441 gcagaaagac gacctggtag aaacctgcat tcggttaaac accacgcacg ttgccatgca
     7501 gcgtacgaag aaggccaaga acggccgcct ggtgacggta tccgagggtg aagccttgat
     7561 tagccgctac aagatcgtaa agagcgaaac cgggcggccg gagtacatcg agatcgagct
     7621 agctgattgg atgtaccgcg agatcacaga aggcaagaac ccggacgtgc tgacggttca
     7681 ccccgattac tttttgatcg atcccggcat cggccgtttt ctctaccgcc tggcacgccg
     7741 cgccgcaggc aaggcagaag ccagatggtt gttcaagacg atctacgaac gcagtggcag
     7801 cgccggagag ttcaagaagt tctgtttcac cgtgcgcaag ctgatcgggt caaatgacct
     7861 gccggagtac gatttgaagg aggaggcggg gcaggctggc ccgatcctag tcatgcgcta
     7921 ccgcaacctg atcgagggcg aagcatccgc cggttcctaa tgtacggagc agatgctagg
     7981 gcaaattgcc ctagcagggg aaaaaggtcg aaaaggtctc tttcctgtgg atagcacgta
     8041 cattgggaac ccaaagccgt acattgggaa ccggaacccg tacattggga acccaaagcc
     8101 gtacattggg aaccggtcac acatgtaagt gactgatata aaagagaaaa aaggcgattt
     8161 ttccgcctaa aactctttaa aacttattaa aactcttaaa acccgcctgg cctgtgcata
     8221 actgtctggc cagcgcacag ccgaagagct gcaaaaagcg cctacccttc ggtcgctgcg
     8281 ctccctacgc cccgccgctt cgcgtcggcc tatcgcggcc gctggccgct caaaaatggc
     8341 tggcctacgg ccaggcaatc taccagggcg cggacaagcc gcgccgtcgc cactcgaccg
     8401 ccggcgccca catcaaggca ccctgcctcg cgcgtttcgg tgatgacggt gaaaacctct
     8461 gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     8521 aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt
     8581 cacgtagcga tagcggagtg tatactggct taactatgcg gcatcagagc agattgtact
     8641 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8701 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg
     8761 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc
     8821 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
     8881 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
     8941 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
     9001 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
     9061 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
     9121 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
     9181 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
     9241 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
     9301 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa
     9361 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg
     9421 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga
     9481 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg
     9541 gctgatgaat cccctaatga tttttatcaa aatcattaag ttaaggtaga tacacatctt
     9601 gtcatatgat caaatggttt cgccaaaaat caataatcag acaacaaaat gtgcgaactc
     9661 gatattttac acgactctct ttaccaattc tgccccgaat tacacttaaa acgactcaac
     9721 agcttaacgt tggcttgcca cgccttactt gactgtaaaa ctctcactct taccgaactt
     9781 ggccgtaacc tgccaaccaa agcgagaaca aaacataaca tcaaacgaat cgaccgattg
     9841 ttaggtaatc gtcacctcca caaagagcga ctcgctgtat accgttggca tgctagcttt
     9901 atctgttcgg gcaatacgat gcccattgta cttgttgact ggtctgatat ccgtgagcaa
     9961 aaacggctta tggtattgcg agcttcagtc gcactacacg gtcgttctgt tactctttat
    10021 gagaaagcgt tcccgctttc agagcaatgt tcaaagaaag ctcatgacca atttctagcc
    10081 gaccttgcga gcattctacc gagtaacacc acaccgctca ttgtcagtga tgctggcttt
    10141 aaagtgccat ggattctagc aactaactta cctgttgaaa ttcgaacacc caaacaactt
    10201 gttaatatct attcgaagcg aatgcagatt gaagaaacct tccgagactt gaaaagtcct
    10261 gcctacggac taggcctacg ccatagccga acgagcagct cagagcgttt tgatatcatg
    10321 ctgctaatcg ccctgatgct tcaactaaca tgttggcttg cgggcgttca tgctcagaaa
    10381 caaggttggg acaagcactt ccaggctaac acagtcagaa atcgaaacgt actctcaaca
    10441 gttcgcttag gcatggaagt tttgcggcat tctggctaca caataacaag ggaagactca
    10501 ctcgtggctg caaccctgct tactcaaaat ctattcacac atggttacgt tttggggaaa
    10561 ttatgagggg atctctcagc gttaagggat tttggtcatg cattctaggt actaaaacaa
    10621 ttcatccagt aaaatataat attttatttt ctcccaatca ggcttgatcc ccagtaagtc
    10681 aaaaaatagc tcgacatact gttcttcccc gatatcctcc ctgatcgacc ggacgcagaa
    10741 ggcaatgtca taccacttgt ccgccctgcc gcttctccca agatcaataa agccacttac
    10801 tttgccatct ttcacaaaga tgttgctgtc tcccaggtcg ccgtgggaaa agacaagttc
    10861 ctcttcgggc ttttccgtct ttaaaaaatc atacagctcg cgcggatctt taaatggagt
    10921 gtcttcttcc cagttttcgc aatccacatc ggccagatcg ttattcagta agtaatccaa
    10981 ttcggctaag cggctgtcta agctattcgt atagggacaa tccgatatgt cgatggagtg
    11041 aaagagcctg atgcactccg catacagctc gataatcttt tcagggcttt gttcatcttc
    11101 atactcttcc gagcaaagga cgccatcggc ctcactcatg agcagattgc tccagccatc
    11161 atgccgttca aagtgcagga cctttggaac aggcagcttt ccttccagcc atagcatcat
    11221 gtccttttcc cgttccacat cataggtggt ccctttatac cggctgtccg tcatttttaa
    11281 atataggttt tcattttctc ccaccagctt atatacctta gcaggagaca ttccttccgt
    11341 atcttttacg cagcggtatt tttcgatcag ttttttcaat tccggtgata ttctcatttt
    11401 agccatttat tatttccttc ctcttttcta cagtatttaa agatacccca agaagctaat
    11461 tataacaaga cgaactccaa ttcactgttc cttgcattct aaaaccttaa ataccagaaa
    11521 acagcttttt caaagttgtt ttcaaagttg gcgtataaca tagtatcgac ggagccgatt
    11581 ttgaaaccgc ggtgatcaca ggcagcaacg ctctgtcatc gttacaatca acatgctacc
    11641 ctccgcgaga tcatccgtgt ttcaaacccg gcagcttagt tgccgttctt ccgaatagca
    11701 tcggtaacat gagcaaagtc tgccgcctta caacggctct cccgctgacg ccgtcccgga
    11761 ctgatgggct gcctgtatcg agtggtgatt ttgtgccgag ctgccggtcg gggagctgtt
    11821 ggctggctgg
//
