LOCUS       sequence-469145-       11976 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  TRV2 expressing Int13.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11976)
  AUTHORS   Chamness JC, Cody JP, Cruz AJ, Voytas DF
  TITLE     Viral delivery of recombinases activates heritable genetic switches 
            in plants.
  JOURNAL   Plant Physiol. 2025 Mar 1;197(3):kiaf073. doi: 
            10.1093/plphys/kiaf073. Epub 2025 Feb 21.
  PUBMED    40111273
REFERENCE   2  (bases 1 to 11976)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2025-03-1"; volume: "197(3):kiaf073. doi"; pages: 
            " 10.1093/plphys/kiaf073. Epub 2025 Feb 21"
COMMENT     Sequence Label: pJPC063
FEATURES             Location/Qualifiers
     source          1..11976
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    1..25
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     promoter        554..899
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     842..862
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     CDS             4324..4341
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      4376..4628
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     misc_feature    4650..4674
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     CDS             5975..6604
                     /codon_start=1
                     /product="stability protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 StaA
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
     CDS             7033..8106
                     /codon_start=1
                     /product="replication protein from the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
                     /label=pVS1 RepA
                     /translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
                     QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
                     KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
                     GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
                     ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
                     LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
                     LVMRYRNLIEGEASAGS"
     rep_origin      8172..8366
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid 
                     pVS1 (Heeb et al., 2000)"
     primer_bind     complement(8602..8624)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     misc_feature    8710..8850
                     /label=bom
                     /note="basis of mobility region from pBR322"
     primer_bind     8765..8784
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(8865..8882)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9036..9624)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9116..9135)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10758..11552)
                     /codon_start=1
                     /gene="aphA-3"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
                     TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
                     EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
                     PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
                     FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
ORIGIN
        1 tggcaggata tattgtggtg taaacaaatt gacgcttaga caacttaata acacattgcg
       61 gacgttttta atgtactgaa ttaacgccga attaattcct aggccaccat gttgggcccg
      121 gcgcgccaag cttgcatgcc tgcaggtcaa catggtggag cacgacactc tcgtctactc
      181 caagaatatc aaagatacag tctcagaaga ccagagggct attgagactt ttcaacaaag
      241 ggtaatatcg ggaaacctcc tcggattcca ttgcccagct atctgtcact tcatcgaaag
      301 gacagtagaa aaggaagatg gcttctacaa atgccatcat tgcgataaag gaaaggctat
      361 cgttcaagat gcctctaccg acagtggtcc caaagatgga cccccaccca cgaggaacat
      421 cgtggaaaaa gaagacgttc caaccacgtc ttcaaagcaa gtggattgat gtgatggtca
      481 acatggtgga gcacgacact ctcgtctact ccaagaatat caaagataca gtctcagaag
      541 accagagggc tattgagact tttcaacaaa gggtaatatc gggaaacctc ctcggattcc
      601 attgcccagc tatctgtcac ttcatcgaaa ggacagtaga aaaggaagat ggcttctaca
      661 aatgccatca ttgcgataaa ggaaaggcta tcgttcaaga tgcctctacc gacagtggtc
      721 ccaaagatgg acccccaccc acgaggaaca tcgtggaaaa agaagacgtt ccaaccacgt
      781 cttcaaagca agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc
      841 actatccttc gcaagaccct tcctctatat aaggaagttc atttcatttg gagaggataa
      901 aacattgcac ctatggtgtt gccctggctg gggtatgtca gtgatcgcag tagaatgtac
      961 taattgacaa gttggagaat acggtagaac gtccttatcc aacacagcct ttatccctct
     1021 ccctgacgag gtttttgtca gtgtaatatt tctttttgaa ctatccagct tagtaccgta
     1081 cgggaaagtg actggtgtgc ttatctttga aatgttactt tgggtttcgg ttctttaggt
     1141 tagtaagaaa gcacttgtct tctcatacaa aggaaaacct gagacgtatc gcttacgaaa
     1201 gtagcaatga aagaaaggtg gtggttttaa tcgctaccgc aaaaacgatg gggtcgtttt
     1261 aattaacttc tcctacgcaa gcgtctaaac ggacgttggg gttttgctag tttctttaga
     1321 gaaaactagc taagtcttta atgttatcat tagagatggc ataaatataa tacttgtgtc
     1381 tgctgataag atcattttaa tttggacgat tagacttgtt gaactacagg ttactgaatc
     1441 acttgcgcta atcaacatgg gagatatgta cgatgaatca tttgacaagt cgggcggtcc
     1501 tgctgacttg atggacgatt cttgggtgga atcagtttcg tggaaagatc tgttgaagaa
     1561 gttacacagc ataaaatttg cactacagtc tggtagagat gagatcactg ggttactagc
     1621 ggcactgaat agacagtgtc cttattcacc atatgagcag tttccagata agaaggtgta
     1681 tttcctttta gactcacggg ctaacagtgc tcttggtgtg attcagaacg cttcagcgtt
     1741 caagagacga gctgatgaga agaatgcagt ggcgggtgtt acaaatattc ctgcgaatcc
     1801 aaacacaacg gttacgacga accaagggag tactactact accaaggcga acactggctc
     1861 gactttggaa gaagacttgt acacttatta caaattcgat gatgcctcta cagctttcca
     1921 caaatctcta acttcgttag agaacatgga gttgaagagt tattaccgaa ggaactttga
     1981 gaaagtattc gggattaagt ttggtggagc agctgctagt tcatctgcac cgcctccagc
     2041 gagtggaggt ccgatacgtc ctaatcccta gggatttaag gacgtgaact ctgttgagat
     2101 ctctgtgaaa ttcagagggt gggtgatacc atattcactg atgccattag cgacatctaa
     2161 atagggctaa ttgtgactaa tttgagggaa tttcctttac cattgacgtc agtgtcgttg
     2221 gtagcatttg agtttcggag catcttgttc tggggtttca cactatcttt agagaaagtg
     2281 ttaagttaat taagttatct taattaagag cataattata ctgatttgtc tctcgttgat
     2341 agagtctatc attctgttac taaaaatttg acaactcggt ttgctgacct actggttact
     2401 gtatcactta cccgagttaa cgagtctaga aacatggccg tgggtatcta tattcgtgtt
     2461 agcacccaag aacaggcaag cgaaggtcat agcattgaaa gccagaaaaa aaagctggcg
     2521 agctattgtg aaattcaggg ttgggatgat tatcgcttct atattgaaga aggcatcagc
     2581 ggcaaaaata ccaatcgtcc gaaactgaaa ctgctgatgg aacatatcga aaagggcaaa
     2641 attaacatcc tgctggttta tcgtctggat cgtctgaccc gtagcgttat tgatctgcat
     2701 aagctgctga attttctgca agaacatggt tgtgcattta aaagcgcaac cgaaacctat
     2761 gataccacca ccgcaaatgg tcgtatgagc atgggtattg ttagcctgct ggcacagtgg
     2821 gaaaccgaaa atatgagcga acgtattaaa ctgaacctgg aacacaaagt tctggttgaa
     2881 ggtgaacgtg ttggtgcaat tccgtatggt tttgatctgt ccgatgatga gaaactggtg
     2941 aaaaatgaaa aaagcgccat tctgctggat atggttgaac gtgtggaaaa tggttggagc
     3001 gttaatcgca ttgtgaatta tctgaatctg accaacaatg accgtaattg gagcccgaat
     3061 ggtgttctgc gtctgctgcg taatccggca ctgtatggtg caacccgttg gaatgataaa
     3121 attgccgaaa atacgcacga aggcattatt agcaaagaac gttttaatcg cctgcagcag
     3181 attctggcag atcgtagcat tcatcatcgt cgtgatgtta aaggcaccta tatctttcag
     3241 ggtgtgctgc gttgtccggt ttgtgatcag accctgagcg tgaatcgttt tatcaaaaaa
     3301 cgtaaagatg gcaccgaata ttgtggtgtg ctgtatcgtt gtcagccgtg tattaaacag
     3361 aataaataca atctggccat tggcgaagca cgttttctga aagcactgaa tgaatatatg
     3421 agcaccgttg aatttcagac cgttgaagat gaagtgatcc cgaaaaaaag cgaacgtgaa
     3481 atgctggaaa gccagctgca gcaaattgca cgtaaacgtg aaaaatatca gaaagcatgg
     3541 gcaagcgatc tgatgagcga cgatgaattt gaaaaactga tggttgaaac ccgtgaaacc
     3601 tacgatgaat gtaaacagaa actggaaagc tgtgaggatc cgatcaaaat cgatgaaacc
     3661 tatctgaaag aaattgtgta tatgtttcac cagaccttta acgatctgga aagcgaaaaa
     3721 cagaaagaat ttatcagcaa gttcattcgc accattcgct ataccgttaa agaacagcag
     3781 ccgattcgtc cggataaaag caaaaccggt aaaggcaaac agaaggtgat tattaccgag
     3841 gtggaattct accagtaaag tgccgggcat gtcccgaaga cattaaacta cggttcttta
     3901 agtagatccg tgtctgaagt tttaggttca atttaaacct acgagattga cattctcgac
     3961 tgatcttgat tgatcggtaa gtcttttgta atttaatttt ctttttgatt ttattttaaa
     4021 ttgttatctg tttctgtgta tagactgttt gagatcggcg tttggccgac tcattgtctt
     4081 accatagggg aacggacttt gtttgtgttg ttattttatt tgtattttat taaaattctc
     4141 aacgatctga aaaagcctcg cggctaagag attgttgggg ggtgagtaag tacttttaaa
     4201 gtgatgatgg ttacaaaggc aaaaggggta aaacccctcg cctacgtaag cgttattacg
     4261 cccgtctgta cttatatcag tacactgacg agtccctaaa ggacgaaacg ggagaacgct
     4321 agccaccacc accaccacca cgtgtgaatt acaggtgacc agctcgaatt tccccgatcg
     4381 ttcaaacatt tggcaataaa gtttcttaag attgaatcct gttgccggtc ttgcgatgat
     4441 tatcatataa tttctgttga attacgttaa gcatgtaata attaacatgt aatgcatgac
     4501 gttatttatg agatgggttt ttatgattag agtcccgcaa ttatacattt aatacgcgat
     4561 agaaaacaaa atatagcgcg caaactagga taaattatcg cgcgcggtgt catctatgtt
     4621 actagatcgg gaattaaact atcagtgttt gacaggatat attggcgggt aaacctaaga
     4681 gaaaagagcg tttattagaa taatcggata tttaaaaggg cgtgaaaagg tttatccgtt
     4741 cgtccatttg tatgtgcatg ccaaccacag ggttcccctc gggatcaaag tactttgatc
     4801 caacccctcc gctgctatag tgcagtcggc ttctgacgtt cagtgcagcc gtcttctgaa
     4861 aacgacatgt cgcacaagtc ctaagttacg cgacaggctg ccgccctgcc cttttcctgg
     4921 cgttttcttg tcgcgtgttt tagtcgcata aagtagaata cttgcgacta gaaccggaga
     4981 cattacgcca tgaacaagag cgccgccgct ggcctgctgg gctatgcccg cgtcagcacc
     5041 gacgaccagg acttgaccaa ccaacgggcc gaactgcacg cggccggctg caccaagctg
     5101 ttttccgaga agatcaccgg caccaggcgc gaccgcccgg agctggccag gatgcttgac
     5161 cacctacgcc ctggcgacgt tgtgacagtg accaggctag accgcctggc ccgcagcacc
     5221 cgcgacctac tggacattgc cgagcgcatc caggaggccg gcgcgggcct gcgtagcctg
     5281 gcagagccgt gggccgacac caccacgccg gccggccgca tggtgttgac cgtgttcgcc
     5341 ggcattgccg agttcgagcg ttccctaatc atcgaccgca cccggagcgg gcgcgaggcc
     5401 gccaaggccc gaggcgtgaa gtttggcccc cgccctaccc tcaccccggc acagatcgcg
     5461 cacgcccgcg agctgatcga ccaggaaggc cgcaccgtga aagaggcggc tgcactgctt
     5521 ggcgtgcatc gctcgaccct gtaccgcgca cttgagcgca gcgaggaagt gacgcccacc
     5581 gaggccaggc ggcgcggtgc cttccgtgag gacgcattga ccgaggccga cgccctggcg
     5641 gccgccgaga atgaacgcca agaggaacaa gcatgaaacc gcaccaggac ggccaggacg
     5701 aaccgttttt cattaccgaa gagatcgagg cggagatgat cgcggccggg tacgtgttcg
     5761 agccgcccgc gcacgtctca accgtgcggc tgcatgaaat cctggccggt ttgtctgatg
     5821 ccaagctggc ggcctggccg gccagcttgg ccgctgaaga aaccgagcgc cgccgtctaa
     5881 aaaggtgatg tgtatttgag taaaacagct tgcgtcatgc ggtcgctgcg tatatgatgc
     5941 gatgagtaaa taaacaaata cgcaagggga acgcatgaag gttatcgctg tacttaacca
     6001 gaaaggcggg tcaggcaaga cgaccatcgc aacccatcta gcccgcgccc tgcaactcgc
     6061 cggggccgat gttctgttag tcgattccga tccccagggc agtgcccgcg attgggcggc
     6121 cgtgcgggaa gatcaaccgc taaccgttgt cggcatcgac cgcccgacga ttgaccgcga
     6181 cgtgaaggcc atcggccggc gcgacttcgt agtgatcgac ggagcgcccc aggcggcgga
     6241 cttggctgtg tccgcgatca aggcagccga cttcgtgctg attccggtgc agccaagccc
     6301 ttacgacata tgggccaccg ccgacctggt ggagctggtt aagcagcgca ttgaggtcac
     6361 ggatggaagg ctacaagcgg cctttgtcgt gtcgcgggcg atcaaaggca cgcgcatcgg
     6421 cggtgaggtt gccgaggcgc tggccgggta cgagctgccc attcttgagt cccgtatcac
     6481 gcagcgcgtg agctacccag gcactgccgc cgccggcaca accgttcttg aatcagaacc
     6541 cgagggcgac gctgcccgcg aggtccaggc gctggccgct gaaattaaat caaaactcat
     6601 ttgagttaat gaggtaaaga gaaaatgagc aaaagcacaa acacgctaag tgccggccgt
     6661 ccgagcgcac gcagcagcaa ggctgcaacg ttggccagcc tggcagacac gccagccatg
     6721 aagcgggtca actttcagtt gccggcggag gatcacacca agctgaagat gtacgcggta
     6781 cgccaaggca agaccattac cgagctgcta tctgaataca tcgcgcagct accagagtaa
     6841 atgagcaaat gaataaatga gtagatgaat tttagcggct aaaggaggcg gcatggaaaa
     6901 tcaagaacaa ccaggcaccg acgccgtgga atgccccatg tgtggaggaa cgggcggttg
     6961 gccaggcgta agcggctggg ttgcctgccg gccctgcaat ggcactggaa cccccaagcc
     7021 cgaggaatcg gcgtgagcgg tcgcaaacca tccggcccgg tacaaatcgg cgcggcgctg
     7081 ggtgatgacc tggtggagaa gttgaaggcc gcgcaggccg cccagcggca acgcatcgag
     7141 gcagaagcac gccccggtga atcgtggcaa gcggccgctg atcgaatccg caaagaatcc
     7201 cggcaaccgc cggcagccgg tgcgccgtcg attaggaagc cgcccaaggg cgacgagcaa
     7261 ccagattttt tcgttccgat gctctatgac gtgggcaccc gcgatagtcg cagcatcatg
     7321 gacgtggccg ttttccgtct gtcgaagcgt gaccgacgag ctggcgaggt gatccgctac
     7381 gagcttccag acgggcacgt agaggtttcc gcagggccgg ccggcatggc cagtgtgtgg
     7441 gattacgacc tggtactgat ggcggtttcc catctaaccg aatccatgaa ccgataccgg
     7501 gaagggaagg gagacaagcc cggccgcgtg ttccgtccac acgttgcgga cgtactcaag
     7561 ttctgccggc gagccgatgg cggaaagcag aaagacgacc tggtagaaac ctgcattcgg
     7621 ttaaacacca cgcacgttgc catgcagcgt acgaagaagg ccaagaacgg ccgcctggtg
     7681 acggtatccg agggtgaagc cttgattagc cgctacaaga tcgtaaagag cgaaaccggg
     7741 cggccggagt acatcgagat cgagctagct gattggatgt accgcgagat cacagaaggc
     7801 aagaacccgg acgtgctgac ggttcacccc gattactttt tgatcgatcc cggcatcggc
     7861 cgttttctct accgcctggc acgccgcgcc gcaggcaagg cagaagccag atggttgttc
     7921 aagacgatct acgaacgcag tggcagcgcc ggagagttca agaagttctg tttcaccgtg
     7981 cgcaagctga tcgggtcaaa tgacctgccg gagtacgatt tgaaggagga ggcggggcag
     8041 gctggcccga tcctagtcat gcgctaccgc aacctgatcg agggcgaagc atccgccggt
     8101 tcctaatgta cggagcagat gctagggcaa attgccctag caggggaaaa aggtcgaaaa
     8161 ggtctctttc ctgtggatag cacgtacatt gggaacccaa agccgtacat tgggaaccgg
     8221 aacccgtaca ttgggaaccc aaagccgtac attgggaacc ggtcacacat gtaagtgact
     8281 gatataaaag agaaaaaagg cgatttttcc gcctaaaact ctttaaaact tattaaaact
     8341 cttaaaaccc gcctggcctg tgcataactg tctggccagc gcacagccga agagctgcaa
     8401 aaagcgccta cccttcggtc gctgcgctcc ctacgccccg ccgcttcgcg tcggcctatc
     8461 gcggccgctg gccgctcaaa aatggctggc ctacggccag gcaatctacc agggcgcgga
     8521 caagccgcgc cgtcgccact cgaccgccgg cgcccacatc aaggcaccct gcctcgcgcg
     8581 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     8641 tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     8701 gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac
     8761 tatgcggcat cagagcagat tgtactgaga gtgcaccata tgcggtgtga aataccgcac
     8821 agatgcgtaa ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg
     8881 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
     8941 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
     9001 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
     9061 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
     9121 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
     9181 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
     9241 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
     9301 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
     9361 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
     9421 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca
     9481 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
     9541 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
     9601 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
     9661 cagtggaacg aaaactcacg ttaagggctg atgaatcccc taatgatttt tatcaaaatc
     9721 attaagttaa ggtagataca catcttgtca tatgatcaaa tggtttcgcc aaaaatcaat
     9781 aatcagacaa caaaatgtgc gaactcgata ttttacacga ctctctttac caattctgcc
     9841 ccgaattaca cttaaaacga ctcaacagct taacgttggc ttgccacgcc ttacttgact
     9901 gtaaaactct cactcttacc gaacttggcc gtaacctgcc aaccaaagcg agaacaaaac
     9961 ataacatcaa acgaatcgac cgattgttag gtaatcgtca cctccacaaa gagcgactcg
    10021 ctgtataccg ttggcatgct agctttatct gttcgggcaa tacgatgccc attgtacttg
    10081 ttgactggtc tgatatccgt gagcaaaaac ggcttatggt attgcgagct tcagtcgcac
    10141 tacacggtcg ttctgttact ctttatgaga aagcgttccc gctttcagag caatgttcaa
    10201 agaaagctca tgaccaattt ctagccgacc ttgcgagcat tctaccgagt aacaccacac
    10261 cgctcattgt cagtgatgct ggctttaaag tgccatggat tctagcaact aacttacctg
    10321 ttgaaattcg aacacccaaa caacttgtta atatctattc gaagcgaatg cagattgaag
    10381 aaaccttccg agacttgaaa agtcctgcct acggactagg cctacgccat agccgaacga
    10441 gcagctcaga gcgttttgat atcatgctgc taatcgccct gatgcttcaa ctaacatgtt
    10501 ggcttgcggg cgttcatgct cagaaacaag gttgggacaa gcacttccag gctaacacag
    10561 tcagaaatcg aaacgtactc tcaacagttc gcttaggcat ggaagttttg cggcattctg
    10621 gctacacaat aacaagggaa gactcactcg tggctgcaac cctgcttact caaaatctat
    10681 tcacacatgg ttacgttttg gggaaattat gaggggatct ctcagcgtta agggattttg
    10741 gtcatgcatt ctaggtacta aaacaattca tccagtaaaa tataatattt tattttctcc
    10801 caatcaggct tgatccccag taagtcaaaa aatagctcga catactgttc ttccccgata
    10861 tcctccctga tcgaccggac gcagaaggca atgtcatacc acttgtccgc cctgccgctt
    10921 ctcccaagat caataaagcc acttactttg ccatctttca caaagatgtt gctgtctccc
    10981 aggtcgccgt gggaaaagac aagttcctct tcgggctttt ccgtctttaa aaaatcatac
    11041 agctcgcgcg gatctttaaa tggagtgtct tcttcccagt tttcgcaatc cacatcggcc
    11101 agatcgttat tcagtaagta atccaattcg gctaagcggc tgtctaagct attcgtatag
    11161 ggacaatccg atatgtcgat ggagtgaaag agcctgatgc actccgcata cagctcgata
    11221 atcttttcag ggctttgttc atcttcatac tcttccgagc aaaggacgcc atcggcctca
    11281 ctcatgagca gattgctcca gccatcatgc cgttcaaagt gcaggacctt tggaacaggc
    11341 agctttcctt ccagccatag catcatgtcc ttttcccgtt ccacatcata ggtggtccct
    11401 ttataccggc tgtccgtcat ttttaaatat aggttttcat tttctcccac cagcttatat
    11461 accttagcag gagacattcc ttccgtatct tttacgcagc ggtatttttc gatcagtttt
    11521 ttcaattccg gtgatattct cattttagcc atttattatt tccttcctct tttctacagt
    11581 atttaaagat accccaagaa gctaattata acaagacgaa ctccaattca ctgttccttg
    11641 cattctaaaa ccttaaatac cagaaaacag ctttttcaaa gttgttttca aagttggcgt
    11701 ataacatagt atcgacggag ccgattttga aaccgcggtg atcacaggca gcaacgctct
    11761 gtcatcgtta caatcaacat gctaccctcc gcgagatcat ccgtgtttca aacccggcag
    11821 cttagttgcc gttcttccga atagcatcgg taacatgagc aaagtctgcc gccttacaac
    11881 ggctctcccg ctgacgccgt cccggactga tgggctgcct gtatcgagtg gtgattttgt
    11941 gccgagctgc cggtcgggga gctgttggct ggctgg
//
