LOCUS       sequence-469193-       10580 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-17xTAcontains 
            17 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10580)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10580)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-17xTA
FEATURES             Location/Qualifiers
     source          1..10580
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1445..1447,1448..2161)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1487..1508)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1748..1767)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2095..2116
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2215..2803
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2268..2288)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2686..2697)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2806..2822)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2807..2823)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2913..3146
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3171..3189)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3172..3189)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3479..3498)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3712..3731)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3831..3853
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3891..3909)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3977..4081
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4082..4150,4151..4942)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4300..4319)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5113..5701
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5602..5621
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5855..5872
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5942..5962)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5947..6276
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6127..6262
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7410..7475
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7605..7625
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8050..8184
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8087..8106)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8141..8160
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8352..8985
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9032..9157
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9654..9887
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10072..10116
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10401..10518
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcaattc tcagccacca tgactgcaac
      241 ggccactgct actgccacag ccacagccac agccactgct acagctacag ccacagctac
      301 cgccaccgcc accgctacag caaccgcgac tgctggagga tcaggcggat catccgaaaa
      361 gcctttacag gtggccagag tggaaagcag tgtcccacaa acaaaggaac tgggaggcat
      421 caccaccctt gatgaccccc taggacaaac gccggaactt ttcgatgcct ttatctgcta
      481 ctgccccaac gatatcgagt ttgtgcagga gatgatccgg caactagaac agacagacta
      541 tcggcttaag ttgtgtgtgt ccgaccgtga cgtcctgccg ggcacctgtg tctggtccat
      601 tgccagcgag ctaattgaga aaaggtgtcg ccgcatggtg gtggttgttt ctgacgatta
      661 tctacagagc aaggaatgtg acttccagac caagtttgca ctcagcctgt ctccaggtgt
      721 ccaacagaag cgactgattc ctattaaata caaggcgatg aagaaggact ttcccagtat
      781 cctgcggttc atcactatat gcgactatac caacccttgc accaagtcct ggttctggac
      841 ccgccttgcc aaggctttgt ccctgcccgg aggtagtggc gggtcccctc cttctcccca
      901 agaaaactcc tatgtcagct ccactggtag ggctcactcc ggagccgcac catggcagcc
      961 actcgctgca ccatcaggtg cttcagccca ggccgcagag caactgcaac ggggacccaa
     1021 ccagccagtc gagtccgacg aaagtcttgg agggctgagc gctgctctgc ggtcctggca
     1081 ccttacacca agttgtcccc ttgatcctgc tcctctgcgg gaggctgggt gcccccaagg
     1141 tgacacagca ggggaatcct catggggttc aggacctggg agccggccaa ccgctgttga
     1201 aggcctggct ttgggctcct ctgcatcatc atcatcagaa cctccccaga ttatcatcaa
     1261 tccagctcgc cagaaaatgg ttcagaaatt ggctttgtat gaggatggtg ccctggattc
     1321 cttgcaattg ctgtcatctt cttctcttcc cggacttgga cttgaacaag acagacaagg
     1381 tccagaggag tccgacgagt tccagagcgg aggttccggg ggtactgggg gtagtggagg
     1441 aagtgtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
     1501 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
     1561 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
     1621 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
     1681 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
     1741 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
     1801 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
     1861 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
     1921 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
     1981 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
     2041 ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg atcacatggt
     2101 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtacaagta
     2161 agcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat cgataatcaa
     2221 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2281 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2341 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2401 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     2461 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     2521 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     2581 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     2641 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     2701 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     2761 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata ccgtcgacct
     2821 cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga
     2881 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     2941 acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     3001 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     3061 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     3121 ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat tctatagtgt
     3181 cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc gcgttctaac
     3241 gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac cctatcatct
     3301 ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt ttctggtaac
     3361 gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc tagccagatc
     3421 ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc
     3481 tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct
     3541 tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc
     3601 ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc
     3661 ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca gtacaatctg
     3721 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
     3781 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
     3841 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
     3901 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
     3961 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
     4021 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
     4081 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
     4141 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
     4201 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
     4261 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
     4321 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
     4381 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
     4441 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
     4501 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
     4561 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
     4621 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
     4681 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
     4741 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
     4801 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
     4861 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     4921 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     4981 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     5041 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     5101 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     5161 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     5221 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     5281 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     5341 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     5401 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     5461 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     5521 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     5581 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     5641 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     5701 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     5761 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     5821 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     5881 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     5941 tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6001 caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
     6061 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc gcccctaact
     6121 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
     6181 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
     6241 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa gacaggcttg
     6301 cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg cgtaaaaaga
     6361 cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc tcgcgcaacc
     6421 tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac ttcacatgag
     6481 cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac tcgcaagccg
     6541 actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg gtctgtaccg
     6601 ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg tatttccagc
     6661 tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg cgatggcgaa
     6721 ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc gattcgtgaa
     6781 atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg tccgctggtt
     6841 gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg ggatatgggc
     6901 gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc actgccgggc
     6961 gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc tggaggctgc
     7021 atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta aacatcctga
     7081 aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc agtcggccct
     7141 tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt ggatctggcg
     7201 cggcattgac ccacgcgaaa tcctcgacat ccaggcacgt attgtgatga gcgatgccga
     7261 acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca actggattta
     7321 tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca taattggaca
     7381 aactacctac agagatttaa agctctaagg taaatataaa atttttaagt gtataatgtg
     7441 ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga actgatgaat
     7501 gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa gaaatgccat
     7561 ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa aagaagagaa
     7621 aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt catgctgtgt
     7681 ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa gctgcactgc
     7741 tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat aacagttata
     7801 atcataacat actgtttttt cttactccac acaggcatag agtgtctgct attaataact
     7861 atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat aaggaatatt
     7921 tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt agaggtttta
     7981 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     8041 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     8101 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     8161 aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa tcatcccaaa
     8221 cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt actctaaacc
     8281 tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat atgtactaca
     8341 aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc ttgatctgtg
     8401 gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag ggccaggggt
     8461 cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc cagataaggt
     8521 agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc tgcatgggat
     8581 ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag catttcatca
     8641 cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag cttgctacaa
     8701 gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg gggagtggcg
     8761 agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct ctctggttag
     8821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     9001 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     9061 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     9121 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9181 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9241 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9301 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9361 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9421 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9481 caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga tatgagggac
     9541 aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt aggagtagca
     9601 cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg aataggagct
     9661 ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc aatgacgctg
     9721 acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa tttgctgagg
     9781 gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa gcagctccag
     9841 gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg gatttggggt
     9901 tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg gagtaataaa
     9961 tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga aattaacaat
    10021 tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa
    10081 caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa cataacaaat
    10141 tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg tttaagaata
    10201 gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc attatcgttt
    10261 cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga agaagaaggt
    10321 ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg gtatcgccaa
    10381 atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg
    10441 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
    10501 acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt ttggatcgat
    10561 aagcttgata tcgaattcag
//
