LOCUS       sequence-469197-       10545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-11xTAcontains 
            11 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10545)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-11xTA
FEATURES             Location/Qualifiers
     source          1..10545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1403..1405,1406..2119)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1445..1466)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1706..1725)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2053..2074
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2180..2768
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2233..2253)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2651..2662)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2771..2787)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2772..2788)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2878..3111
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3136..3154)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3137..3154)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3444..3463)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3677..3696)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3796..3818
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3856..3874)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3942..4046
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4047..4115,4116..4907)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4265..4284)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5078..5666
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5567..5586
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5820..5837
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5907..5927)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5912..6241
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6092..6227
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7375..7440
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7570..7590
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8015..8149
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8052..8071)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8106..8125
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8317..8950
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8997..9122
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9619..9852
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10037..10081
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10366..10483
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgacag ctaccgctac
      241 tgccactgct actgccactg caaccgccac tgccacagca actgcaaccg ctggaggctc
      301 tggcggtagc agcgagaagc ctttacaggt ggccagagtg gaaagcagtg tcccacaaac
      361 aaaggaactg ggaggcatca ccacccttga tgacccccta ggacaaacgc cggaactttt
      421 cgatgccttt atctgctact gccccaacga tatcgagttt gtgcaggaga tgatccggca
      481 actagaacag acagactatc ggcttaagtt gtgtgtgtcc gaccgtgacg tcctgccggg
      541 cacctgtgtc tggtccattg ccagcgagct aattgagaaa aggtgtcgcc gcatggtggt
      601 ggttgtttct gacgattatc tacagagcaa ggaatgtgac ttccagacca agtttgcact
      661 cagcctgtct ccaggtgtcc aacagaagcg actgattcct attaaataca aggcgatgaa
      721 gaaggacttt cccagtatcc tgcggttcat cactatatgc gactatacca acccttgcac
      781 caagtcctgg ttctggaccc gccttgccaa ggctttgtcc ctgcccggag gtagtggcgg
      841 gtcccctcct tctccccaag aaaactccta tgtcagctcc actggtaggg ctcactccgg
      901 agccgcacca tggcagccac tcgctgcacc atcaggtgct tcagcccagg ccgcagagca
      961 actgcaacgg ggacccaacc agccagtcga gtccgacgaa agtcttggag ggctgagcgc
     1021 tgctctgcgg tcctggcacc ttacaccaag ttgtcccctt gatcctgctc ctctgcggga
     1081 ggctgggtgc ccccaaggtg acacagcagg ggaatcctca tggggttcag gacctgggag
     1141 ccggccaacc gctgttgaag gcctggcttt gggctcctct gcatcatcat catcagaacc
     1201 tccccagatt atcatcaatc cagctcgcca gaaaatggtt cagaaattgg ctttgtatga
     1261 ggatggtgcc ctggattcct tgcaattgct gtcatcttct tctcttcccg gacttggact
     1321 tgaacaagac agacaaggtc cagaggagtc cgacgagttc cagagcggag gttccggggg
     1381 tactgggggt agtggaggaa gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1441 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1501 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1561 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1621 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1681 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1741 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1801 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1861 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1921 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     1981 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     2041 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2101 ggacgagctg tacaagtaaa gcggccgcga ctctagagtc gacctgcagg catgcaagct
     2161 tgatatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
     2221 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
     2281 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
     2341 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
     2401 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
     2461 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
     2521 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaaatcatc
     2581 gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
     2641 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
     2701 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
     2761 ctccccgcat cgataccgtc gacctcgagg gaattaattc gagctcggta cctttaagac
     2821 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     2881 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     2941 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     3001 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     3061 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agcatctaga
     3121 attaattccg tgtattctat agtgtcacct aaatcgtatg tgtatgatac ataaggttat
     3181 gtattaattg tagccgcgtt ctaacgacaa tatgtacaag cctaattgtg tagcatctgg
     3241 cttactgaag cagaccctat catctctctc gtaaactgcc gtcagagtcg gtttggttgg
     3301 acgaaccttc tgagtttctg gtaacgccgt cccgcacccg gaaatggtca gcgaaccaat
     3361 cagcagggtc atcgctagcc agatcctcta cgccggacgc atcgtggccg gcatcaccgg
     3421 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc
     3481 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc
     3541 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa
     3601 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg
     3661 aatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     3721 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     3781 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     3841 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     3901 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     3961 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     4021 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     4081 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     4141 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     4201 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     4261 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     4321 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     4381 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     4441 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     4501 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     4561 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     4621 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     4681 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     4741 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     4801 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     4861 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     4921 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     4981 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     5041 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     5101 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     5161 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     5221 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     5281 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     5341 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     5401 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     5461 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     5521 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     5581 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     5641 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     5701 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     5761 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     5821 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     5881 gttggccgat tcattaatgc agctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     5941 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     6001 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     6061 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     6121 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     6181 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     6241 aaagcttgga cacaagacag gcttgcgaga tatgtttgag aataccactt tatcccgcgt
     6301 cagggagagg cagtgcgtaa aaagacgcgg actcatgtga aatactggtt tttagtgcgc
     6361 cagatctcta taatctcgcg caacctattt tcccctcgaa cactttttaa gccgtagata
     6421 aacaggctgg gacacttcac atgagcgaaa aatacatcgt cacctgggac atgttgcaga
     6481 tccatgcacg taaactcgca agccgactga tgccttctga acaatggaaa ggcattattg
     6541 ccgtaagccg tggcggtctg taccgggtgc gttactggcg cgtgaactgg gtattcgtca
     6601 tgtcgatacc gtttgtattt ccagctacga tcacgacaac cagcgcgagc ttaaagtgct
     6661 gaaacgcgca gaaggcgatg gcgaaggctt catcgttatt gatgacctgg tggataccgg
     6721 tggtactgcg gttgcgattc gtgaaatgta tccaaaagcg cactttgtca ccatcttcgc
     6781 aaaaccggct ggtcgtccgc tggttgatga ctatgttgtt gatatcccgc aagatacctg
     6841 gattgaacag ccgtgggata tgggcgtcgt attcgtcccg ccaatctccg gtcgctaatc
     6901 ttttcaacgc ctggcactgc cgggcgttgt tctttttaac ttcaggcggg ttacaatagt
     6961 ttccagtaag tattctggag gctgcatcca tgacacaggc aaacctgagc gaaaccctgt
     7021 tcaaaccccg ctttaaacat cctgaaacct cgacgctagt ccgccgcttt aatcacggcg
     7081 cacaaccgcc tgtgcagtcg gcccttgatg gtaaaaccat ccctcactgg tatcgcatga
     7141 ttaaccgtct gatgtggatc tggcgcggca ttgacccacg cgaaatcctc gacgtccagg
     7201 cacgtattgt gatgagcgat gccgaacgta ccgacgatga tttatacgat acggtgattg
     7261 gctaccgtgg cggcaactgg atttatgagt gggccccgga tctttgtgaa ggaaccttac
     7321 ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc taaggtaaat
     7381 ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg tgtattttag
     7441 attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa tgaggaaaac
     7501 ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga ctctcaacat
     7561 tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc ttcagaattg
     7621 ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt tgctatttac
     7681 accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata ttctgtaacc
     7741 tttataagta ggcataacag ttataatcat aacatactgt tttttcttac tccacacagg
     7801 catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag ctttttaatt
     7861 tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga tcataatcag
     7921 ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc tccccctgaa
     7981 cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag cttataatgg
     8041 ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt cactgcattc
     8101 tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatca actggataac
     8161 tcaagctaac caaaatcatc ccaaacttcc caccccatac cctattacca ctgccaatta
     8221 cctgtggttt catttactct aaacctgtga ttcctctgaa ttattttcat tttaaagaaa
     8281 ttgtatttgt taaatatgta ctacaaactt agtagttgga agggctaatt cactcccaaa
     8341 gaagacaaga tatccttgat ctgtggatct accacacaca aggctacttc cctgattagc
     8401 agaactacac accagggcca ggggtcagat atccactgac ctttggatgg tgctacaagc
     8461 tagtaccagt tgagccagat aaggtagaag aggccaataa aggagagaac accagcttgt
     8521 tacaccctgt gagcctgcat gggatggatg acccggagag agaagtgtta gagtggaggt
     8581 ttgacagccg cctagcattt catcacgtgg cccgagagct gcatccggag tacttcaaga
     8641 actgctgata tcgagcttgc tacaagggac tttccgctgg ggactttcca gggaggcgtg
     8701 gcctgggcgg gactggggag tggcgagccc tcagatcctg catataagca gctgcttttt
     8761 gcctgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     8821 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     8881 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     8941 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     9001 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     9061 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     9121 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     9181 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     9241 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     9301 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     9361 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     9421 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccggccgc tgatcttcag
     9481 acctggagga ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt
     9541 aaaaattgaa ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga
     9601 aaaaagagca gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac
     9661 tatgggcgca gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt
     9721 gcagcagcag aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac
     9781 agtctggggc atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga
     9841 tcaacagctc ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc
     9901 ttggaatgct agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat
     9961 ggagtgggac agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc
    10021 gcaaaaccag caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt
    10081 gtggaattgg tttaacataa caaattggct gtggtatata aaattattca taatgatagt
    10141 aggaggcttg gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag
    10201 gcagggatat tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag
    10261 gcccgaagga atagaagaag aaggtggaga gagagacaga gacagatcca ttcgattagt
    10321 gaacggatct cgacggtatc gccaaatggc agtattcatc cacaatttta aaagaaaagg
    10381 ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa cagacataca
    10441 aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt attacaggga
    10501 cagcagagat ccagtttgga tcgataagct tgatatcgaa ttcag
//
