LOCUS       sequence-469198-       10461 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-3xA which 
            contains 3 TRAF6 binding motif with a alanine mutation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10461)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10461)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-3xA
FEATURES             Location/Qualifiers
     source          1..10461
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1130..1132,1133..1843)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1172..1193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1433..1452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1780..1801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2096..2684
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2149..2169)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2567..2578)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2687..2703)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2688..2704)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2794..3027
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3052..3070)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3053..3070)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3360..3379)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3593..3612)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3712..3734
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3772..3790)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3858..3962
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3963..4031,4032..4823)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4181..4200)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4994..5582
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5483..5502
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5736..5753
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5823..5843)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5828..6157
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6008..6143
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7291..7356
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7486..7506
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7931..8065
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7968..7987)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8022..8041
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8233..8866
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8913..9038
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9535..9768
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9953..9997
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10282..10399
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgtctg cgggagaccc
      241 ccgcgtggga tccgggtccc tggactcctt catgttctcc atacccttgg tcgcgcttaa
      301 cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct cggacgcccg tggcggccga
      361 ctggaccttg ctggcggagg agatgggctt cgagtacttg gagatccgag agctggaaac
      421 gcgccctgac cccactcgca gtttgttgga tgcctggcag gggcgctctg gcgcgtctgt
      481 cggcaggctg ctagagctgc tggccttgtt agaccgtgag gatatactga aggagctgaa
      541 gtcgcgcatc gaggaggact gccagaaata cttaggtaag cagcagaacc aggagtccga
      601 gaagccttta caggtggcca gagtggaaag cagtgtccca caaacaaagg aactgggagg
      661 catcaccacc cttgatgacc ccctaggaca aacgccggaa cttttcgatg cctttatctg
      721 ctactgcccc aacgatatcg agtttgtgca ggagatgatc cggcaactag aacagacaga
      781 ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg ccgggcacct gtgtctggtc
      841 cattgccagc gagctaattg agaaaaggtg tcgccgcatg gtggtggttg tttctgacga
      901 ttatctacag agcaaggaat gtgacttcca gaccaagttt gcactcagcc tgtctccagg
      961 tgtccaacag aagcgactga ttcctattaa atacaaggcg atgaagaagg actttcccag
     1021 tatcctgcgg ttcatcacta tatgcgacta taccaaccct tgcaccaagt cctggttctg
     1081 gacccgcctt gccaaggctt tgtccctgcc cggaggtagt ggcgggtccg tgagcaaggg
     1141 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1201 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1261 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1321 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1381 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1441 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1501 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1561 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1621 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1741 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1801 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtggca gtcctaagca
     1861 ggaacctcaa gcaatcgact tcggtggggg ctcaggtgga actgggtcag gtggcggttc
     1921 tggaggtcca aaacaagaac cacaggcgat agactttgga ggcggcagcg gaggcaccgg
     1981 tagtggggga acagggggta gtcccaagca agagccccaa gcaatagact tttaaagcgg
     2041 ccgcgactct agagtcgacc tgcaggcatg caagcttgat atcaagctta tcgataatca
     2101 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
     2161 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
     2221 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
     2281 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
     2341 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
     2401 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
     2461 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
     2521 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
     2581 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
     2641 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
     2701 tcgagggaat taattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag
     2761 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     2821 gacaagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     2881 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2941 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     3001 tttagtcagt gtggaaaatc tctagcagca tctagaatta attccgtgta ttctatagtg
     3061 tcacctaaat cgtatgtgta tgatacataa ggttatgtat taattgtagc cgcgttctaa
     3121 cgacaatatg tacaagccta attgtgtagc atctggctta ctgaagcaga ccctatcatc
     3181 tctctcgtaa actgccgtca gagtcggttt ggttggacga accttctgag tttctggtaa
     3241 cgccgtcccg cacccggaaa tggtcagcga accaatcagc agggtcatcg ctagccagat
     3301 cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     3361 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     3421 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     3481 cttgcatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     3541 cttcctaatg caggagtcgc ataagggaga gcgtcgaatg gtgcactctc agtacaatct
     3601 gctctgatgc cgcatagtta agccagcccc gacacccgcc aacacccgct gacgcgccct
     3661 gacgggcttg tctgctcccg gcatccgctt acagacaagc tgtgaccgtc tccgggagct
     3721 gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc gagacgaaag ggcctcgtga
     3781 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
     3841 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     3901 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     3961 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     4021 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     4081 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     4141 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     4201 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     4261 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     4321 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     4381 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     4441 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     4501 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     4561 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     4621 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     4681 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     4741 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     4801 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     4861 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     4921 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     4981 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     5041 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     5101 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     5161 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     5221 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     5281 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     5341 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     5401 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     5461 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     5521 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     5581 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     5641 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     5701 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     5761 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     5821 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     5881 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     5941 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6001 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6061 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     6121 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag cttggacaca agacaggctt
     6181 gcgagatatg tttgagaata ccactttatc ccgcgtcagg gagaggcagt gcgtaaaaag
     6241 acgcggactc atgtgaaata ctggttttta gtgcgccaga tctctataat ctcgcgcaac
     6301 ctattttccc ctcgaacact ttttaagccg tagataaaca ggctgggaca cttcacatga
     6361 gcgaaaaata catcgtcacc tgggacatgt tgcagatcca tgcacgtaaa ctcgcaagcc
     6421 gactgatgcc ttctgaacaa tggaaaggca ttattgccgt aagccgtggc ggtctgtacc
     6481 gggtgcgtta ctggcgcgtg aactgggtat tcgtcatgtc gataccgttt gtatttccag
     6541 ctacgatcac gacaaccagc gcgagcttaa agtgctgaaa cgcgcagaag gcgatggcga
     6601 aggcttcatc gttattgatg acctggtgga taccggtggt actgcggttg cgattcgtga
     6661 aatgtatcca aaagcgcact ttgtcaccat cttcgcaaaa ccggctggtc gtccgctggt
     6721 tgatgactat gttgttgata tcccgcaaga tacctggatt gaacagccgt gggatatggg
     6781 cgtcgtattc gtcccgccaa tctccggtcg ctaatctttt caacgcctgg cactgccggg
     6841 cgttgttctt tttaacttca ggcgggttac aatagtttcc agtaagtatt ctggaggctg
     6901 catccatgac acaggcaaac ctgagcgaaa ccctgttcaa accccgcttt aaacatcctg
     6961 aaacctcgac gctagtccgc cgctttaatc acggcgcaca accgcctgtg cagtcggccc
     7021 ttgatggtaa aaccatccct cactggtatc gcatgattaa ccgtctgatg tggatctggc
     7081 gcggcattga cccacgcgaa atcctcgacg tccaggcacg tattgtgatg agcgatgccg
     7141 aacgtaccga cgatgattta tacgatacgg tgattggcta ccgtggcggc aactggattt
     7201 atgagtgggc cccggatctt tgtgaaggaa ccttacttct gtggtgtgac ataattggac
     7261 aaactaccta cagagattta aagctctaag gtaaatataa aatttttaag tgtataatgt
     7321 gttaaactac tgattctaat tgtttgtgta ttttagattc caacctatgg aactgatgaa
     7381 tgggagcagt ggtggaatgc ctttaatgag gaaaacctgt tttgctcaga agaaatgcca
     7441 tctagtgatg atgaggctac tgctgactct caacattcta ctcctccaaa aaagaagaga
     7501 aaggtagaag accccaagga ctttccttca gaattgctaa gttttttgag tcatgctgtg
     7561 tttagtaata gaactcttgc ttgctttgct atttacacca caaaggaaaa agctgcactg
     7621 ctatacaaga aaattatgga aaaatattct gtaaccttta taagtaggca taacagttat
     7681 aatcataaca tactgttttt tcttactcca cacaggcata gagtgtctgc tattaataac
     7741 tatgctcaaa aattgtgtac ctttagcttt ttaatttgta aaggggttaa taaggaatat
     7801 ttgatgtata gtgccttgac tagagatcat aatcagccat accacatttg tagaggtttt
     7861 acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa tgaatgcaat
     7921 tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     7981 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     8041 caatgtatct tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa
     8101 acttcccacc ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac
     8161 ctgtgattcc tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac
     8221 aaacttagta gttggaaggg ctaattcact cccaaagaag acaagatatc cttgatctgt
     8281 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
     8341 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
     8401 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
     8461 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
     8521 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
     8581 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
     8641 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
     8701 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
     8761 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
     8821 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca
     8881 gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact cggcttgctg
     8941 aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa attttgacta
     9001 gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg gggagaatta
     9061 gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat aaattaaaac
     9121 atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc ctgttagaaa
     9181 catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag acaggatcag
     9241 aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat caaaggatag
     9301 agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac aaaagtaaga
     9361 ccaccgcaca gcaagcggcc ggccgctgat cttcagacct ggaggaggag atatgaggga
     9421 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     9481 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     9541 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     9601 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     9661 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     9721 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     9781 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     9841 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     9901 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     9961 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
    10021 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
    10081 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
    10141 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
    10201 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcca
    10261 aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg
    10321 ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat
    10381 tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccag tttggatcga
    10441 taagcttgat atcgaattca g
//
