LOCUS       sequence-46920-e         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Johannessen CM, Boehm JS, Kim SY, Thomas SR, Wardwell L, Johnson LA,
            Emery CM, Stransky N, Cogdill AP, Barretina J, Caponigro G, 
            Hieronymus H, Murray RR, Salehi-Ashtiani K, Hill DE, Vidal M, Zhao 
            JJ, Yang X, Alkan O, Kim S, Harris JL, Wilson CJ, Myer VE, Finan PM,
            Root DE, Roberts TM, Golub T, Flaherty KT, Dummer R, Weber BL, 
            Sellers WR, Schlegel R, Wargo JA, Hahn WC, Garraway LA
  TITLE     COT drives resistance to RAF inhibition through MAP kinase pathway 
            reactivation.
  JOURNAL   Nature. 2010 Dec 16;468(7326):968-72. doi: 10.1038/nature09627
  PUBMED    21107320
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nature"; date: "2010-12-16- 16"; volume: "468"; issue: "7326"; 
            pages: "968-72"
COMMENT     Sequence Label: pDONR223-MAPK1
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    17..116
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(118..153)
                     /codon_start=1
                     /product="epitope tag derived from MAP kinase 2"
                     /label=Tag-100
                     /translation="EETARFQPGYRS"
ORIGIN
        1 cacgccgcgc tggatggcaa taatgatttt attttgactg atagtgacct gttcgttgca
       61 acaaattgat aagcaatgct ttcttataat gccaactttg tacaagaaag ttggcaaaga
      121 tctgtatcct ggctggaatc tagcagtctc ttcaaaaatt agttctttga gcttttcctt
      181 aggcaagtca tccaattcca tgtcgaactt gaatggtgct tcggcgatgg gctcgtcact
      241 cgggtcgtaa tactgctcca gatatgggtg ggccagagcc tgttctactt caatcctctt
      301 gtgtgggttg aatgtcaaca ttttgtccaa taagtccaga gctttggagt cagcatttgg
      361 gaacagcctg ttccatggca ccttattttt gtgtggaaga gaaagcaaat agttcctagc
      421 ttttaaattt attatacaat tcaggtcttc ttgtgatggg gatccaagaa tacccaaaat
      481 gtggttcagc tggtcaagat aatgcttccc tggaaagatg ggcctgttag aaagcatttc
      541 tgccagaatg cagcctacag accaaatatc aatggacttg gtgtagccct tggaattcaa
      601 cataatttct ggagccctgt accaacgtgt ggccacatat tctgtcagga accctgtgtg
      661 atcatggtct ggatctgcaa cacgggccac gccaaagtca cagatcttga gatcacagtg
      721 gtgttgagca gcatgttgga aggcttgagg t
//
