LOCUS       sequence-469200-       10830 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS three mutations
            in the death domain that disrupt oligomer formation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-V43D/E52K/R62E
FEATURES             Location/Qualifiers
     source          1..10830
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1688..1690,1691..2404)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1730..1751)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1991..2010)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2338..2359
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2465..3053
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2518..2538)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2936..2947)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3056..3072)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3057..3073)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3163..3396
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3421..3439)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3422..3439)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3729..3748)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3962..3981)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4081..4103
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4141..4159)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4227..4331
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4332..4400,4401..5192)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4550..4569)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5363..5951
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5852..5871
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6105..6122
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6192..6212)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6197..6526
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6377..6512
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7660..7725
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7855..7875
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8300..8434
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8337..8356)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8391..8410
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8602..9235
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9282..9407
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9904..10137
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10322..10366
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10651..10768
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgtctg cgggagaccc
      241 ccgcgtggga tccgggtccc tggactcctt catgttctcc atacccttgg tcgcgcttaa
      301 cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct cggacgcccg acgcggccga
      361 ctggaccttg ctggcgaagg agatgggctt cgagtacttg gagatcgaag agctggaaac
      421 gcgccctgac cccactcgca gtttgttgga tgcctggcag gggcgctctg gcgcgtctgt
      481 cggcaggctg ctagagctgc tggccttgtt agaccgtgag gatatactga aggagctgaa
      541 gtcgcgcatc gaggaggact gccagaaata cttaggtaag cagcagaacc aggagtccga
      601 gaagccttta caggtggcca gagtggaaag cagtgtccca caaacaaagg aactgggagg
      661 catcaccacc cttgatgacc ccctaggaca aacgccggaa cttttcgatg cctttatctg
      721 ctactgcccc aacgatatcg agtttgtgca ggagatgatc cggcaactag aacagacaga
      781 ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg ccgggcacct gtgtctggtc
      841 cattgccagc gagctaattg agaaaaggtg tcgccgcatg gtggtggttg tttctgacga
      901 ttatctacag agcaaggaat gtgacttcca gaccaagttt gcactcagcc tgtctccagg
      961 tgtccaacag aagcgactga ttcctattaa atacaaggcg atgaagaagg actttcccag
     1021 tatcctgcgg ttcatcacta tatgcgacta taccaaccct tgcaccaagt cctggttctg
     1081 gacccgcctt gccaaggctt tgtccctgcc cggaggtagt ggcgggtccc ctccttctcc
     1141 ccaagaaaac tcctatgtca gctccactgg tagggctcac tccggagccg caccatggca
     1201 gccactcgct gcaccatcag gtgcttcagc ccaggccgca gagcaactgc aacggggacc
     1261 caaccagcca gtcgagtccg acgaaagtct tggagggctg agcgctgctc tgcggtcctg
     1321 gcaccttaca ccaagttgtc cccttgatcc tgctcctctg cgggaggctg ggtgccccca
     1381 aggtgacaca gcaggggaat cctcatgggg ttcaggacct gggagccggc caaccgctgt
     1441 tgaaggcctg gctttgggct cctctgcatc atcatcatca gaacctcccc agattatcat
     1501 caatccagct cgccagaaaa tggttcagaa attggctttg tatgaggatg gtgccctgga
     1561 ttccttgcaa ttgctgtcat cttcttctct tcccggactt ggacttgaac aagacagaca
     1621 aggtccagag gagtccgacg agttccagag cggaggttcc gggggtactg ggggtagtgg
     1681 aggaagtgtg agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct
     1741 ggacggcgac gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac
     1801 ctacggcaag ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc
     1861 caccctcgtg accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat
     1921 gaagcagcac gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat
     1981 cttcttcaag gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac
     2041 cctggtgaac cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg
     2101 gcacaagctg gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa
     2161 gaacggcatc aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct
     2221 cgccgaccac taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa
     2281 ccactacctg agcacccagt ccaagctgag caaagacccc aacgagaagc gcgatcacat
     2341 ggtcctgctg gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa
     2401 gtaaagcggc cgcgactcta gagtcgacct gcaggcatgc aagcttgata tcaagcttat
     2461 cgataatcaa cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt
     2521 tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc
     2581 ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga
     2641 gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc
     2701 cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct
     2761 ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     2821 gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct
     2881 gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc
     2941 cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg
     3001 tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcatcgata
     3061 ccgtcgacct cgagggaatt aattcgagct cggtaccttt aagaccaatg acttacaagg
     3121 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     3181 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     3241 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     3301 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     3361 tcagaccctt ttagtcagtg tggaaaatct ctagcagcat ctagaattaa ttccgtgtat
     3421 tctatagtgt cacctaaatc gtatgtgtat gatacataag gttatgtatt aattgtagcc
     3481 gcgttctaac gacaatatgt acaagcctaa ttgtgtagca tctggcttac tgaagcagac
     3541 cctatcatct ctctcgtaaa ctgccgtcag agtcggtttg gttggacgaa ccttctgagt
     3601 ttctggtaac gccgtcccgc acccggaaat ggtcagcgaa ccaatcagca gggtcatcgc
     3661 tagccagatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt
     3721 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct
     3781 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg
     3841 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact
     3901 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaatgg tgcactctca
     3961 gtacaatctg ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg
     4021 acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct
     4081 ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg
     4141 gcctcgtgat acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt
     4201 caggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     4261 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     4321 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     4381 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     4441 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     4501 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     4561 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     4621 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     4681 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     4741 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     4801 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     4861 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     4921 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     4981 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     5041 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     5101 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     5161 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     5221 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     5281 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     5341 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     5401 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     5461 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     5521 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     5581 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     5641 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     5701 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     5761 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     5821 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     5881 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     5941 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6001 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     6061 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     6121 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     6181 aatgcagctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     6241 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     6301 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     6361 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     6421 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     6481 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttggacacaa
     6541 gacaggcttg cgagatatgt ttgagaatac cactttatcc cgcgtcaggg agaggcagtg
     6601 cgtaaaaaga cgcggactca tgtgaaatac tggtttttag tgcgccagat ctctataatc
     6661 tcgcgcaacc tattttcccc tcgaacactt tttaagccgt agataaacag gctgggacac
     6721 ttcacatgag cgaaaaatac atcgtcacct gggacatgtt gcagatccat gcacgtaaac
     6781 tcgcaagccg actgatgcct tctgaacaat ggaaaggcat tattgccgta agccgtggcg
     6841 gtctgtaccg ggtgcgttac tggcgcgtga actgggtatt cgtcatgtcg ataccgtttg
     6901 tatttccagc tacgatcacg acaaccagcg cgagcttaaa gtgctgaaac gcgcagaagg
     6961 cgatggcgaa ggcttcatcg ttattgatga cctggtggat accggtggta ctgcggttgc
     7021 gattcgtgaa atgtatccaa aagcgcactt tgtcaccatc ttcgcaaaac cggctggtcg
     7081 tccgctggtt gatgactatg ttgttgatat cccgcaagat acctggattg aacagccgtg
     7141 ggatatgggc gtcgtattcg tcccgccaat ctccggtcgc taatcttttc aacgcctggc
     7201 actgccgggc gttgttcttt ttaacttcag gcgggttaca atagtttcca gtaagtattc
     7261 tggaggctgc atccatgaca caggcaaacc tgagcgaaac cctgttcaaa ccccgcttta
     7321 aacatcctga aacctcgacg ctagtccgcc gctttaatca cggcgcacaa ccgcctgtgc
     7381 agtcggccct tgatggtaaa accatccctc actggtatcg catgattaac cgtctgatgt
     7441 ggatctggcg cggcattgac ccacgcgaaa tcctcgacgt ccaggcacgt attgtgatga
     7501 gcgatgccga acgtaccgac gatgatttat acgatacggt gattggctac cgtggcggca
     7561 actggattta tgagtgggcc ccggatcttt gtgaaggaac cttacttctg tggtgtgaca
     7621 taattggaca aactacctac agagatttaa agctctaagg taaatataaa atttttaagt
     7681 gtataatgtg ttaaactact gattctaatt gtttgtgtat tttagattcc aacctatgga
     7741 actgatgaat gggagcagtg gtggaatgcc tttaatgagg aaaacctgtt ttgctcagaa
     7801 gaaatgccat ctagtgatga tgaggctact gctgactctc aacattctac tcctccaaaa
     7861 aagaagagaa aggtagaaga ccccaaggac tttccttcag aattgctaag ttttttgagt
     7921 catgctgtgt ttagtaatag aactcttgct tgctttgcta tttacaccac aaaggaaaaa
     7981 gctgcactgc tatacaagaa aattatggaa aaatattctg taacctttat aagtaggcat
     8041 aacagttata atcataacat actgtttttt cttactccac acaggcatag agtgtctgct
     8101 attaataact atgctcaaaa attgtgtacc tttagctttt taatttgtaa aggggttaat
     8161 aaggaatatt tgatgtatag tgccttgact agagatcata atcagccata ccacatttgt
     8221 agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     8281 gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     8341 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8401 caaactcatc aatgtatctt atcatgtctg gatcaactgg ataactcaag ctaaccaaaa
     8461 tcatcccaaa cttcccaccc cataccctat taccactgcc aattacctgt ggtttcattt
     8521 actctaaacc tgtgattcct ctgaattatt ttcattttaa agaaattgta tttgttaaat
     8581 atgtactaca aacttagtag ttggaagggc taattcactc ccaaagaaga caagatatcc
     8641 ttgatctgtg gatctaccac acacaaggct acttccctga ttagcagaac tacacaccag
     8701 ggccaggggt cagatatcca ctgacctttg gatggtgcta caagctagta ccagttgagc
     8761 cagataaggt agaagaggcc aataaaggag agaacaccag cttgttacac cctgtgagcc
     8821 tgcatgggat ggatgacccg gagagagaag tgttagagtg gaggtttgac agccgcctag
     8881 catttcatca cgtggcccga gagctgcatc cggagtactt caagaactgc tgatatcgag
     8941 cttgctacaa gggactttcc gctggggact ttccagggag gcgtggcctg ggcgggactg
     9001 gggagtggcg agccctcaga tcctgcatat aagcagctgc tttttgcctg tactgggtct
     9061 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     9121 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     9181 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     9241 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     9301 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     9361 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     9421 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     9481 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     9541 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     9601 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     9661 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     9721 aaagtaagac caccgcacag caagcggccg gccgctgatc ttcagacctg gaggaggaga
     9781 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
     9841 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
     9901 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
     9961 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
    10021 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
    10081 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
    10141 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
    10201 gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt gggacagaga
    10261 aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa accagcaaga
    10321 aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga attggtttaa
    10381 cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag gcttggtagg
    10441 tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg gatattcacc
    10501 attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg aaggaataga
    10561 agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg gatctcgacg
    10621 gtatcgccaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
    10681 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
    10741 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccagt
    10801 ttggatcgat aagcttgata tcgaattcag
//
