LOCUS       sequence-469202-       10611 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-5x which 
            contains 5 TRAF6 binding motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10611)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10611)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-5x
FEATURES             Location/Qualifiers
     source          1..10611
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1130..1132,1133..1843)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1172..1193)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1433..1452)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1780..1801
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2246..2834
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2299..2319)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2717..2728)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2837..2853)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2838..2854)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2944..3177
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3202..3220)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3203..3220)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3510..3529)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3743..3762)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3862..3884
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3922..3940)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4008..4112
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4113..4181,4182..4973)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4331..4350)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5144..5732
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5633..5652
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5886..5903
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5973..5993)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5978..6307
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6158..6293
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7441..7506
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7636..7656
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8081..8215
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8118..8137)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8172..8191
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8383..9016
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9063..9188
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9685..9918
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10103..10147
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10432..10549
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgtctg cgggagaccc
      241 ccgcgtggga tccgggtccc tggactcctt catgttctcc atacccttgg tcgcgcttaa
      301 cgtgggagtg aggcgccgcc tatcgctgtt cttgaaccct cggacgcccg tggcggccga
      361 ctggaccttg ctggcggagg agatgggctt cgagtacttg gagatccgag agctggaaac
      421 gcgccctgac cccactcgca gtttgttgga tgcctggcag gggcgctctg gcgcgtctgt
      481 cggcaggctg ctagagctgc tggccttgtt agaccgtgag gatatactga aggagctgaa
      541 gtcgcgcatc gaggaggact gccagaaata cttaggtaag cagcagaacc aggagtccga
      601 gaagccttta caggtggcca gagtggaaag cagtgtccca caaacaaagg aactgggagg
      661 catcaccacc cttgatgacc ccctaggaca aacgccggaa cttttcgatg cctttatctg
      721 ctactgcccc aacgatatcg agtttgtgca ggagatgatc cggcaactag aacagacaga
      781 ctatcggctt aagttgtgtg tgtccgaccg tgacgtcctg ccgggcacct gtgtctggtc
      841 cattgccagc gagctaattg agaaaaggtg tcgccgcatg gtggtggttg tttctgacga
      901 ttatctacag agcaaggaat gtgacttcca gaccaagttt gcactcagcc tgtctccagg
      961 tgtccaacag aagcgactga ttcctattaa atacaaggcg atgaagaagg actttcccag
     1021 tatcctgcgg ttcatcacta tatgcgacta taccaaccct tgcaccaagt cctggttctg
     1081 gacccgcctt gccaaggctt tgtccctgcc cggaggtagt ggcgggtccg tgagcaaggg
     1141 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1201 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1261 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1321 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1381 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1441 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1501 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1561 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     1621 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     1681 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     1741 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     1801 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagggtggaa gtcctaaaca
     1861 agaacctcaa gagatcgatt tcggaggagg cagtggcggc acaggatccg gcgggggcag
     1921 cggtgggccc aaacaggaac cacaggaaat tgatttcggc ggcgggtctg gaggtacagg
     1981 tagcgggggc actggaggct ctccaaagca ggagcctcag gagatagact ttggcggggg
     2041 gagcggcggt actggatctg gcggtggcag tggtggccct aagcaggagc cccaagagat
     2101 cgactttggg ggaggcagtg gtgggaccgg atcaggagga actgggggta gtcccaagca
     2161 agaaccacaa gaaatcgact tttaaagcgg ccgcgactct agagtcgacc tgcaggcatg
     2221 caagcttgat atcaagctta tcgataatca acctctggat tacaaaattt gtgaaagatt
     2281 gactggtatt cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc
     2341 tttgtatcat gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg
     2401 gttgctgtct ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac
     2461 tgtgtttgct gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc
     2521 cgggactttc gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc
     2581 ccgctgctgg acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa
     2641 atcatcgtcc tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc
     2701 cttctgctac gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc
     2761 ggctctgcgg cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg
     2821 ggccgcctcc ccgcatcgat accgtcgacc tcgagggaat taattcgagc tcggtacctt
     2881 taagaccaat gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg
     2941 gactggaagg gctaattcac tcccaacgaa gacaagatct gctttttgct tgtactgggt
     3001 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     3061 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     3121 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagca
     3181 tctagaatta attccgtgta ttctatagtg tcacctaaat cgtatgtgta tgatacataa
     3241 ggttatgtat taattgtagc cgcgttctaa cgacaatatg tacaagccta attgtgtagc
     3301 atctggctta ctgaagcaga ccctatcatc tctctcgtaa actgccgtca gagtcggttt
     3361 ggttggacga accttctgag tttctggtaa cgccgtcccg cacccggaaa tggtcagcga
     3421 accaatcagc agggtcatcg ctagccagat cctctacgcc ggacgcatcg tggccggcat
     3481 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     3541 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     3601 cgtggccggg ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt
     3661 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     3721 gcgtcgaatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     3781 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     3841 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     3901 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     3961 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     4021 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     4081 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     4141 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     4201 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     4261 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     4321 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     4381 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     4441 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     4501 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     4561 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     4621 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     4681 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     4741 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     4801 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     4861 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     4921 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     4981 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     5041 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     5101 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     5161 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     5221 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     5281 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     5341 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     5401 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     5461 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     5521 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     5581 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     5641 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     5701 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     5761 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     5821 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     5881 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     5941 ccgcgcgttg gccgattcat taatgcagct gtggaatgtg tgtcagttag ggtgtggaaa
     6001 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     6061 caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     6121 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag
     6181 ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc
     6241 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt
     6301 ttgcaaaaag cttggacaca agacaggctt gcgagatatg tttgagaata ccactttatc
     6361 ccgcgtcagg gagaggcagt gcgtaaaaag acgcggactc atgtgaaata ctggttttta
     6421 gtgcgccaga tctctataat ctcgcgcaac ctattttccc ctcgaacact ttttaagccg
     6481 tagataaaca ggctgggaca cttcacatga gcgaaaaata catcgtcacc tgggacatgt
     6541 tgcagatcca tgcacgtaaa ctcgcaagcc gactgatgcc ttctgaacaa tggaaaggca
     6601 ttattgccgt aagccgtggc ggtctgtacc gggtgcgtta ctggcgcgtg aactgggtat
     6661 tcgtcatgtc gataccgttt gtatttccag ctacgatcac gacaaccagc gcgagcttaa
     6721 agtgctgaaa cgcgcagaag gcgatggcga aggcttcatc gttattgatg acctggtgga
     6781 taccggtggt actgcggttg cgattcgtga aatgtatcca aaagcgcact ttgtcaccat
     6841 cttcgcaaaa ccggctggtc gtccgctggt tgatgactat gttgttgata tcccgcaaga
     6901 tacctggatt gaacagccgt gggatatggg cgtcgtattc gtcccgccaa tctccggtcg
     6961 ctaatctttt caacgcctgg cactgccggg cgttgttctt tttaacttca ggcgggttac
     7021 aatagtttcc agtaagtatt ctggaggctg catccatgac acaggcaaac ctgagcgaaa
     7081 ccctgttcaa accccgcttt aaacatcctg aaacctcgac gctagtccgc cgctttaatc
     7141 acggcgcaca accgcctgtg cagtcggccc ttgatggtaa aaccatccct cactggtatc
     7201 gcatgattaa ccgtctgatg tggatctggc gcggcattga cccacgcgaa atcctcgacg
     7261 tccaggcacg tattgtgatg agcgatgccg aacgtaccga cgatgattta tacgatacgg
     7321 tgattggcta ccgtggcggc aactggattt atgagtgggc cccggatctt tgtgaaggaa
     7381 ccttacttct gtggtgtgac ataattggac aaactaccta cagagattta aagctctaag
     7441 gtaaatataa aatttttaag tgtataatgt gttaaactac tgattctaat tgtttgtgta
     7501 ttttagattc caacctatgg aactgatgaa tgggagcagt ggtggaatgc ctttaatgag
     7561 gaaaacctgt tttgctcaga agaaatgcca tctagtgatg atgaggctac tgctgactct
     7621 caacattcta ctcctccaaa aaagaagaga aaggtagaag accccaagga ctttccttca
     7681 gaattgctaa gttttttgag tcatgctgtg tttagtaata gaactcttgc ttgctttgct
     7741 atttacacca caaaggaaaa agctgcactg ctatacaaga aaattatgga aaaatattct
     7801 gtaaccttta taagtaggca taacagttat aatcataaca tactgttttt tcttactcca
     7861 cacaggcata gagtgtctgc tattaataac tatgctcaaa aattgtgtac ctttagcttt
     7921 ttaatttgta aaggggttaa taaggaatat ttgatgtata gtgccttgac tagagatcat
     7981 aatcagccat accacatttg tagaggtttt acttgcttta aaaaacctcc cacacctccc
     8041 cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta ttgcagctta
     8101 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     8161 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatcaactg
     8221 gataactcaa gctaaccaaa atcatcccaa acttcccacc ccatacccta ttaccactgc
     8281 caattacctg tggtttcatt tactctaaac ctgtgattcc tctgaattat tttcatttta
     8341 aagaaattgt atttgttaaa tatgtactac aaacttagta gttggaaggg ctaattcact
     8401 cccaaagaag acaagatatc cttgatctgt ggatctacca cacacaaggc tacttccctg
     8461 attagcagaa ctacacacca gggccagggg tcagatatcc actgaccttt ggatggtgct
     8521 acaagctagt accagttgag ccagataagg tagaagaggc caataaagga gagaacacca
     8581 gcttgttaca ccctgtgagc ctgcatggga tggatgaccc ggagagagaa gtgttagagt
     8641 ggaggtttga cagccgccta gcatttcatc acgtggcccg agagctgcat ccggagtact
     8701 tcaagaactg ctgatatcga gcttgctaca agggactttc cgctggggac tttccaggga
     8761 ggcgtggcct gggcgggact ggggagtggc gagccctcag atcctgcata taagcagctg
     8821 ctttttgcct gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     8881 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     8941 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     9001 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     9061 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     9121 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     9181 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     9241 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     9301 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     9361 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     9421 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     9481 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc ggccgctgat
     9541 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     9601 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     9661 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     9721 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     9781 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     9841 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     9901 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     9961 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    10021 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    10081 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    10141 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    10201 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    10261 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    10321 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
    10381 attagtgaac ggatctcgac ggtatcgcca aatggcagta ttcatccaca attttaaaag
    10441 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
    10501 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
    10561 cagggacagc agagatccag tttggatcga taagcttgat atcgaattca g
//
