LOCUS       sequence-469205-       10692 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-dDD contains 
            the bacterial death domain from Nostoc. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS_bDD
FEATURES             Location/Qualifiers
     source          1..10692
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1550..1552,1553..2266)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1592..1613)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1853..1872)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2200..2221
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2327..2915
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2380..2400)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2798..2809)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2918..2934)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2919..2935)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3025..3258
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3283..3301)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3284..3301)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3591..3610)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3824..3843)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3943..3965
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4003..4021)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4089..4193
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4194..4262,4263..5054)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4412..4431)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5225..5813
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5714..5733
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5967..5984
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6054..6074)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6059..6388
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6239..6374
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7522..7587
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7717..7737
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8162..8296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8199..8218)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8253..8272
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8464..9097
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9144..9269
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9766..9999
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10184..10228
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10513..10630
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgcaga ctaagatttt
      241 catatgtagg cggttaactc aggattggca ggacctggct gactatttcg aaatacagcc
      301 ccacgaacgt gcggggttta aacctggccg ggaaccacat agcatctggg agtggctgga
      361 acagaggaat cgacttggtg agcttgaatc tgctctgata gagatcggaa gggaggacct
      421 ggcgcaggag ctgaagaaga acggaggtag tggcgggtcc gagaagcctt tacaggtggc
      481 cagagtggaa agcagtgtcc cacaaacaaa ggaactggga ggcatcacca cccttgatga
      541 ccccctagga caaacgccgg aacttttcga tgcctttatc tgctactgcc ccaacgatat
      601 cgagtttgtg caggagatga tccggcaact agaacagaca gactatcggc ttaagttgtg
      661 tgtgtccgac cgtgacgtcc tgccgggcac ctgtgtctgg tccattgcca gcgagctaat
      721 tgagaaaagg tgtcgccgca tggtggtggt tgtttctgac gattatctac agagcaagga
      781 atgtgacttc cagaccaagt ttgcactcag cctgtctcca ggtgtccaac agaagcgact
      841 gattcctatt aaatacaagg cgatgaagaa ggactttccc agtatcctgc ggttcatcac
      901 tatatgcgac tataccaacc cttgcaccaa gtcctggttc tggacccgcc ttgccaaggc
      961 tttgtccctg cccggaggta gtggcgggtc ccctccttct ccccaagaaa actcctatgt
     1021 cagctccact ggtagggctc actccggagc cgcaccatgg cagccactcg ctgcaccatc
     1081 aggtgcttca gcccaggccg cagagcaact gcaacgggga cccaaccagc cagtcgagtc
     1141 cgacgaaagt cttggagggc tgagcgctgc tctgcggtcc tggcacctta caccaagttg
     1201 tccccttgat cctgctcctc tgcgggaggc tgggtgcccc caaggtgaca cagcagggga
     1261 atcctcatgg ggttcaggac ctgggagccg gccaaccgct gttgaaggcc tggctttggg
     1321 ctcctctgca tcatcatcat cagaacctcc ccagattatc atcaatccag ctcgccagaa
     1381 aatggttcag aaattggctt tgtatgagga tggtgccctg gattccttgc aattgctgtc
     1441 atcttcttct cttcccggac ttggacttga acaagacaga caaggtccag aggagtccga
     1501 cgagttccag agcggaggtt ccgggggtac tgggggtagt ggaggaagtg tgagcaaggg
     1561 cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg
     1621 ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct
     1681 gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct
     1741 gacctacggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt
     1801 caagtccgcc atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg
     1861 caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga
     1921 gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa
     1981 ctacaacagc cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa
     2041 cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca
     2101 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca
     2161 gtccaagctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     2221 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc
     2281 tagagtcgac ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga
     2341 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     2401 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     2461 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     2521 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     2581 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     2641 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     2701 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
     2761 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     2821 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca
     2881 gacgagtcgg atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa
     2941 ttaattcgag ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     3001 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     3061 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     3121 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     3181 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     3241 tgtggaaaat ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa
     3301 tcgtatgtgt atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat
     3361 gtacaagcct aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta
     3421 aactgccgtc agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc
     3481 gcacccggaa atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc
     3541 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     3601 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     3661 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     3721 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     3781 gcaggagtcg cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg
     3841 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
     3901 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
     3961 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
     4021 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
     4081 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     4141 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     4201 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     4261 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     4321 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     4381 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     4441 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     4501 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     4561 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     4621 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     4681 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     4741 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     4801 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     4861 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     4921 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     4981 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     5041 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     5101 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     5161 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     5221 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     5281 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     5341 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     5401 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     5461 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     5521 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     5581 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     5641 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     5701 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     5761 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     5821 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     5881 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     5941 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     6001 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt
     6061 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     6121 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     6181 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     6241 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     6301 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     6361 cttttttgga ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat
     6421 gtttgagaat accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact
     6481 catgtgaaat actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc
     6541 cctcgaacac tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat
     6601 acatcgtcac ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc
     6661 cttctgaaca atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt
     6721 actggcgcgt gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca
     6781 cgacaaccag cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat
     6841 cgttattgat gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc
     6901 aaaagcgcac tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta
     6961 tgttgttgat atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt
     7021 cgtcccgcca atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct
     7081 ttttaacttc aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga
     7141 cacaggcaaa cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga
     7201 cgctagtccg ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta
     7261 aaaccatccc tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg
     7321 acccacgcga aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg
     7381 acgatgattt atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg
     7441 ccccggatct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
     7501 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
     7561 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
     7621 tggtggaatg cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat
     7681 gatgaggcta ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa
     7741 gaccccaagg actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat
     7801 agaactcttg cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag
     7861 aaaattatgg aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac
     7921 atactgtttt ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa
     7981 aaattgtgta cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat
     8041 agtgccttga ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt
     8101 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt
     8161 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     8221 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     8281 ttatcatgtc tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac
     8341 cccataccct attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc
     8401 ctctgaatta ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt
     8461 agttggaagg gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc
     8521 acacacaagg ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc
     8581 cactgacctt tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg
     8641 ccaataaagg agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc
     8701 cggagagaga agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc
     8761 gagagctgca tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt
     8821 ccgctgggga ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca
     8881 gatcctgcat ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc
     8941 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     9001 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     9061 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     9121 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     9181 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     9241 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     9301 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     9361 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     9421 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     9481 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     9541 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     9601 agcaagcggc cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
     9661 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
     9721 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
     9781 tgggttcttg ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca
     9841 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
     9901 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
     9961 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
    10021 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
    10081 acagatttgg aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag
    10141 cttaatacac tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt
    10201 attggaatta gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg
    10261 gtatataaaa ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc
    10321 tgtactttct atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca
    10381 cctcccaacc ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag
    10441 agacagagac agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt
    10501 attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat
    10561 agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat
    10621 tcaaaatttt cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga
    10681 tatcgaattc ag
//
