LOCUS       sequence-469210-       10806 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-bDD with 5 
            TRAF6 binding motifs.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10806)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10806)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-bDD-5xT6BMs
FEATURES             Location/Qualifiers
     source          1..10806
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..213
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      72..207
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1666..1668,1669..2382)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1708..1729)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1969..1988)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2316..2337
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2443..3031
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2496..2516)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2914..2925)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3034..3050)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3035..3051)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3141..3374
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3399..3417)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3400..3417)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3707..3726)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3940..3959)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4059..4081
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4119..4137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4205..4309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4310..4378,4379..5170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4528..4547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5341..5929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5830..5849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6083..6100
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6170..6190)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6175..6504
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6355..6490
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7638..7703
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7833..7853
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8278..8412
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8315..8334)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8369..8388
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8580..9213
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9260..9385
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9882..10115
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10300..10344
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10629..10746
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 aggcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
       61 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
      121 gactaatttt ttttatttat gcagaggccg aggccgcctc tgcctctgag ctattccaga
      181 agtagtgagg aggctttttt ggaggcctag gctacgcgtg ccaccatgca gactaagatt
      241 ttcatatgta ggcggttaac tcaggattgg caggacctgg ctgactattt cgaaatacag
      301 ccccacgaac gtgcggggtt taaacctggc cgggaaccac atagcatctg ggagtggctg
      361 gaacagagga atcgacttgg tgagcttgaa tctgctctga tagagatcgg aagggaggac
      421 ctggcgcagg agctgaagaa gaacggaggt agtggcgggt ccgagaagcc tttacaggtg
      481 gccagagtgg aaagcagtgt cccacaaaca aaggaactgg gaggcatcac cacccttgat
      541 gaccccctag gacaaacgcc ggaacttttc gatgccttta tctgctactg ccccaacgat
      601 atcgagtttg tgcaggagat gatccggcaa ctagaacaga cagactatcg gcttaagttg
      661 tgtgtgtccg accgtgacgt cctgccgggc acctgtgtct ggtccattgc cagcgagcta
      721 attgagaaaa ggtgtcgccg catggtggtg gttgtttctg acgattatct acagagcaag
      781 gaatgtgact tccagaccaa gtttgcactc agcctgtctc caggtgtcca acagaagcga
      841 ctgattccta ttaaatacaa ggcgatgaag aaggactttc ccagtatcct gcggttcatc
      901 actatatgcg actataccaa cccttgcacc aagtcctggt tctggacccg ccttgccaag
      961 gctttgtccc tgcccggagg tagtggcggg tcccctcctt ctccccaaga aaactcctat
     1021 gtcagctcca ctggtagggc tcactccgga gccgcaccat ggcagccact cgctgcacca
     1081 tcaggtgctt cagcccaggc cgcagagcaa ctgcaacggg gacccaacca gccagtcgag
     1141 tccgacgaaa gtcttggagg gctgagcgct gctctgcggt cctggcacct tacaccaagt
     1201 tgtccccttg atcctgctcc tctgcgggag gctgggtgcc cccaaggtga cacagcaggg
     1261 gaatcctcat ggggttcagg acctgggagc cggccaaccg ctgttgaagg cctggctttg
     1321 ggctcctctg catcatcatc atcagaacct ccccagatta tcatcaatcc agctcgccag
     1381 aaaatggttc agaaattggc tttgtatgag gatggtgccc tggattcctt gcaattgctg
     1441 tcatcttctt ctcttcccgg acttggactt gaacaagaca gacaaggtcc agaggagtcc
     1501 gacgagttcc agggtggcag tcctaagcag gaacctcaag aaatcgactt cggtgggggc
     1561 tcaggtggaa ctgggtcagg tggcggttct ggaggtccaa aacaagaacc acaggagata
     1621 gactttagcg gaggttccgg gggtactggg ggtagtggag gaagtgtgag caagggcgag
     1681 gagctgttca ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac
     1741 aagttcagcg tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag
     1801 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
     1861 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
     1921 tccgccatgc ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac
     1981 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
     2041 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac
     2101 aacagccaca acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc
     2161 aagatccgcc acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac
     2221 acccccatcg gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc
     2281 aagctgagca aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc
     2341 gccgccggga tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga
     2401 gtcgacctgc aggcatgcaa gcttgatatc aagcttatcg ataatcaacc tctggattac
     2461 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     2521 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     2581 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     2641 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     2701 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     2761 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     2821 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     2881 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     2941 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     3001 agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg agggaattaa
     3061 ttcgagctcg gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt
     3121 tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac aagatctgct
     3181 ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta
     3241 actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg
     3301 tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg
     3361 gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca cctaaatcgt
     3421 atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga caatatgtac
     3481 aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct ctcgtaaact
     3541 gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc cgtcccgcac
     3601 ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct ctacgccgga
     3661 cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta tatcgccgac
     3721 atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg
     3781 ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt gcatgcacca
     3841 ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt cctaatgcag
     3901 gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct ctgatgccgc
     3961 atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac gggcttgtct
     4021 gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca tgtgtcagag
     4081 gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac gcctattttt
     4141 ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa
     4201 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     4261 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     4321 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     4381 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
     4441 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
     4501 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
     4561 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
     4621 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
     4681 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
     4741 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
     4801 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
     4861 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
     4921 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
     4981 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
     5041 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
     5101 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
     5161 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
     5221 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
     5281 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
     5341 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
     5401 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
     5461 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
     5521 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
     5581 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
     5641 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
     5701 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
     5761 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
     5821 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
     5881 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
     5941 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
     6001 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
     6061 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
     6121 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg gaatgtgtgt
     6181 cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat
     6241 ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg
     6301 caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg
     6361 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     6421 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     6481 tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg agatatgttt
     6541 gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg cggactcatg
     6601 tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta ttttcccctc
     6661 gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg aaaaatacat
     6721 cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac tgatgccttc
     6781 tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg tgcgttactg
     6841 gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta cgatcacgac
     6901 aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg cttcatcgtt
     6961 attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat gtatccaaaa
     7021 gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga tgactatgtt
     7081 gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt cgtattcgtc
     7141 ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt tgttcttttt
     7201 aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat ccatgacaca
     7261 ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa cctcgacgct
     7321 agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg atggtaaaac
     7381 catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg gcattgaccc
     7441 acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac gtaccgacga
     7501 tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg agtgggcccc
     7561 ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag
     7621 agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga
     7681 ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt
     7741 ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg
     7801 aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc
     7861 ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa
     7921 ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa
     7981 ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat cataacatac
     8041 tgttttttct tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat
     8101 tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg
     8161 ccttgactag agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa
     8221 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac
     8281 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat
     8341 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat
     8401 catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact tcccacccca
     8461 taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg tgattcctct
     8521 gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa cttagtagtt
     8581 ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga tctaccacac
     8641 acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca gatatccact
     8701 gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag aagaggccaa
     8761 taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg atgacccgga
     8821 gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg tggcccgaga
     8881 gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg gactttccgc
     8941 tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag ccctcagatc
     9001 ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac cagatctgag
     9061 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     9121 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     9181 gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc
     9241 gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc
     9301 aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa
     9361 ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga
     9421 aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc
     9481 aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg
     9541 tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc
     9601 attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac
     9661 caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca
     9721 agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     9781 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     9841 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     9901 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     9961 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
    10021 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
    10081 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
    10141 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
    10201 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
    10261 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
    10321 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
    10381 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
    10441 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
    10501 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
    10561 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccaaat ggcagtattc
    10621 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
    10681 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
    10741 aattttcggg tttattacag ggacagcaga gatccagttt ggatcgataa gcttgatatc
    10801 gaattc
//
