LOCUS       sequence-469213-       10599 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-10xTAcontains 
            20 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10599)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10599)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-20xTA
FEATURES             Location/Qualifiers
     source          1..10599
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1463..1465,1466..2179)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1505..1526)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1766..1785)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2113..2134
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2233..2821
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2286..2306)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2704..2715)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2824..2840)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2825..2841)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2931..3164
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3189..3207)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3190..3207)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3497..3516)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3730..3749)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3849..3871
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3909..3927)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3995..4099
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4100..4168,4169..4960)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4318..4337)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5131..5719
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5620..5639
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5873..5890
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5960..5980)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5965..6294
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6145..6280
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7428..7493
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7623..7643
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8068..8202
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8105..8124)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8159..8178
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8370..9003
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9050..9175
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9672..9905
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10090..10134
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10419..10536
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcaattc tcagccacca tgactgctac
      241 cgccacagct actgcaactg ccacggccac agccacggcc accgcgactg caactgcaac
      301 cgctactgct actgccacag ctaccgcaac tgctactgcc acagcgactg ccggaggatc
      361 aggcggatca tccgaaaagc ctttacaggt ggccagagtg gaaagcagtg tcccacaaac
      421 aaaggaactg ggaggcatca ccacccttga tgacccccta ggacaaacgc cggaactttt
      481 cgatgccttt atctgctact gccccaacga tatcgagttt gtgcaggaga tgatccggca
      541 actagaacag acagactatc ggcttaagtt gtgtgtgtcc gaccgtgacg tcctgccggg
      601 cacctgtgtc tggtccattg ccagcgagct aattgagaaa aggtgtcgcc gcatggtggt
      661 ggttgtttct gacgattatc tacagagcaa ggaatgtgac ttccagacca agtttgcact
      721 cagcctgtct ccaggtgtcc aacagaagcg actgattcct attaaataca aggcgatgaa
      781 gaaggacttt cccagtatcc tgcggttcat cactatatgc gactatacca acccttgcac
      841 caagtcctgg ttctggaccc gccttgccaa ggctttgtcc ctgcccggag gtagtggcgg
      901 gtcccctcct tctccccaag aaaactccta tgtcagctcc actggtaggg ctcactccgg
      961 agccgcacca tggcagccac tcgctgcacc atcaggtgct tcagcccagg ccgcagagca
     1021 actgcaacgg ggacccaacc agccagtcga gtccgacgaa agtcttggag ggctgagcgc
     1081 tgctctgcgg tcctggcacc ttacaccaag ttgtcccctt gatcctgctc ctctgcggga
     1141 ggctgggtgc ccccaaggtg acacagcagg ggaatcctca tggggttcag gacctgggag
     1201 ccggccaacc gctgttgaag gcctggcttt gggctcctct gcatcatcat catcagaacc
     1261 tccccagatt atcatcaatc cagctcgcca gaaaatggtt cagaaattgg ctttgtatga
     1321 ggatggtgcc ctggattcct tgcaattgct gtcatcttct tctcttcccg gacttggact
     1381 tgaacaagac agacaaggtc cagaggagtc cgacgagttc cagagcggag gttccggggg
     1441 tactgggggt agtggaggaa gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     1501 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
     1561 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     1621 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     1681 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     1741 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     1801 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     1861 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     1921 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
     1981 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     2041 gctgctgccc gacaaccact acctgagcac ccagtccaag ctgagcaaag accccaacga
     2101 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     2161 ggacgagctg tacaagtaag cgactctaga gtcgacctgc aggcatgcaa gcttgatatc
     2221 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     2281 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     2341 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     2401 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     2461 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     2521 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     2581 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
     2641 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     2701 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     2761 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     2821 catcgatacc gtcgacctcg agggaattaa ttcgagctcg gtacctttaa gaccaatgac
     2881 ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac tggaagggct
     2941 aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc tctggttaga
     3001 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     3061 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     3121 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagcatct agaattaatt
     3181 ccgtgtattc tatagtgtca cctaaatcgt atgtgtatga tacataaggt tatgtattaa
     3241 ttgtagccgc gttctaacga caatatgtac aagcctaatt gtgtagcatc tggcttactg
     3301 aagcagaccc tatcatctct ctcgtaaact gccgtcagag tcggtttggt tggacgaacc
     3361 ttctgagttt ctggtaacgc cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg
     3421 gtcatcgcta gccagatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca
     3481 ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac
     3541 ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga
     3601 ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc
     3661 aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaatggtg
     3721 cactctcagt acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac
     3781 acccgctgac gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt
     3841 gaccgtctcc gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag
     3901 acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     3961 ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     4021 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     4081 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     4141 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     4201 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     4261 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     4321 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     4381 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     4441 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     4501 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     4561 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     4621 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     4681 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     4741 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     4801 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     4861 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     4921 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     4981 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     5041 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     5101 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     5161 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     5221 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     5281 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     5341 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     5401 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     5461 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     5521 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     5581 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     5641 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     5701 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     5761 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     5821 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     5881 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     5941 gattcattaa tgcagctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     6001 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     6061 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     6121 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     6181 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
     6241 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
     6301 ggacacaaga caggcttgcg agatatgttt gagaatacca ctttatcccg cgtcagggag
     6361 aggcagtgcg taaaaagacg cggactcatg tgaaatactg gtttttagtg cgccagatct
     6421 ctataatctc gcgcaaccta ttttcccctc gaacactttt taagccgtag ataaacaggc
     6481 tgggacactt cacatgagcg aaaaatacat cgtcacctgg gacatgttgc agatccatgc
     6541 acgtaaactc gcaagccgac tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag
     6601 ccgtggcggt ctgtaccggg tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat
     6661 accgtttgta tttccagcta cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc
     6721 gcagaaggcg atggcgaagg cttcatcgtt attgatgacc tggtggatac cggtggtact
     6781 gcggttgcga ttcgtgaaat gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg
     6841 gctggtcgtc cgctggttga tgactatgtt gttgatatcc cgcaagatac ctggattgaa
     6901 cagccgtggg atatgggcgt cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa
     6961 cgcctggcac tgccgggcgt tgttcttttt aacttcaggc gggttacaat agtttccagt
     7021 aagtattctg gaggctgcat ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc
     7081 ccgctttaaa catcctgaaa cctcgacgct agtccgccgc tttaatcacg gcgcacaacc
     7141 gcctgtgcag tcggcccttg atggtaaaac catccctcac tggtatcgca tgattaaccg
     7201 tctgatgtgg atctggcgcg gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat
     7261 tgtgatgagc gatgccgaac gtaccgacga tgatttatac gatacggtga ttggctaccg
     7321 tggcggcaac tggatttatg agtgggcccc ggatctttgt gaaggaacct tacttctgtg
     7381 gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     7441 ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     7501 cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     7561 gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     7621 ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     7681 ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     7741 aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
     7801 gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
     7861 tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
     7921 gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     7981 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     8041 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     8101 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     8161 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tcaactggat aactcaagct
     8221 aaccaaaatc atcccaaact tcccacccca taccctatta ccactgccaa ttacctgtgg
     8281 tttcatttac tctaaacctg tgattcctct gaattatttt cattttaaag aaattgtatt
     8341 tgttaaatat gtactacaaa cttagtagtt ggaagggcta attcactccc aaagaagaca
     8401 agatatcctt gatctgtgga tctaccacac acaaggctac ttccctgatt agcagaacta
     8461 cacaccaggg ccaggggtca gatatccact gacctttgga tggtgctaca agctagtacc
     8521 agttgagcca gataaggtag aagaggccaa taaaggagag aacaccagct tgttacaccc
     8581 tgtgagcctg catgggatgg atgacccgga gagagaagtg ttagagtgga ggtttgacag
     8641 ccgcctagca tttcatcacg tggcccgaga gctgcatccg gagtacttca agaactgctg
     8701 atatcgagct tgctacaagg gactttccgc tggggacttt ccagggaggc gtggcctggg
     8761 cgggactggg gagtggcgag ccctcagatc ctgcatataa gcagctgctt tttgcctgta
     8821 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     8881 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     8941 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     9001 gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg
     9061 caggactcgg cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac
     9121 gccaaaaatt ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat
     9181 taagcggggg agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa
     9241 aaaatataaa ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa
     9301 tcctggcctg ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc
     9361 ccttcagaca ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg
     9421 tgtgcatcaa aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga
     9481 gcaaaacaaa agtaagacca ccgcacagca agcggccggc cgctgatctt cagacctgga
     9541 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     9601 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     9661 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     9721 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     9781 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     9841 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     9901 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     9961 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
    10021 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
    10081 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
    10141 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
    10201 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
    10261 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
    10321 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
    10381 tctcgacggt atcgccaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt
    10441 ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa
    10501 gaattacaaa aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga
    10561 gatccagttt ggatcgataa gcttgatatc gaattccag
//
