LOCUS       sequence-469214-       10569 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-15xTAcontains 
            15 TA repeats in place of the death domain.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10569)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10569)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-15xTA
FEATURES             Location/Qualifiers
     source          1..10569
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1427..1429,1430..2143)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1469..1490)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1730..1749)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2077..2098
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2204..2792
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2257..2277)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2675..2686)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2795..2811)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2796..2812)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2902..3135
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3160..3178)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3161..3178)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3468..3487)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3701..3720)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3820..3842
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3880..3898)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3966..4070
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4071..4139,4140..4931)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4289..4308)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5102..5690
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5591..5610
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5844..5861
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5931..5951)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5936..6265
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6116..6251
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7399..7464
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7594..7614
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8039..8173
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8076..8095)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8130..8149
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8341..8974
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9021..9146
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9643..9876
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10061..10105
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10390..10507
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgacag ccactgcaac
      241 cgcaacagct acagctaccg ccacagcaac cgcaacagca accgcaactg ctactgcaac
      301 agcaacagca accgccggag gatcaggcgg atcatccgaa aagcctttac aggtggccag
      361 agtggaaagc agtgtcccac aaacaaagga actgggaggc atcaccaccc ttgatgaccc
      421 cctaggacaa acgccggaac ttttcgatgc ctttatctgc tactgcccca acgatatcga
      481 gtttgtgcag gagatgatcc ggcaactaga acagacagac tatcggctta agttgtgtgt
      541 gtccgaccgt gacgtcctgc cgggcacctg tgtctggtcc attgccagcg agctaattga
      601 gaaaaggtgt cgccgcatgg tggtggttgt ttctgacgat tatctacaga gcaaggaatg
      661 tgacttccag accaagtttg cactcagcct gtctccaggt gtccaacaga agcgactgat
      721 tcctattaaa tacaaggcga tgaagaagga ctttcccagt atcctgcggt tcatcactat
      781 atgcgactat accaaccctt gcaccaagtc ctggttctgg acccgccttg ccaaggcttt
      841 gtccctgccc ggaggtagtg gcgggtcccc tccttctccc caagaaaact cctatgtcag
      901 ctccactggt agggctcact ccggagccgc accatggcag ccactcgctg caccatcagg
      961 tgcttcagcc caggccgcag agcaactgca acggggaccc aaccagccag tcgagtccga
     1021 cgaaagtctt ggagggctga gcgctgctct gcggtcctgg caccttacac caagttgtcc
     1081 ccttgatcct gctcctctgc gggaggctgg gtgcccccaa ggtgacacag caggggaatc
     1141 ctcatggggt tcaggacctg ggagccggcc aaccgctgtt gaaggcctgg ctttgggctc
     1201 ctctgcatca tcatcatcag aacctcccca gattatcatc aatccagctc gccagaaaat
     1261 ggttcagaaa ttggctttgt atgaggatgg tgccctggat tccttgcaat tgctgtcatc
     1321 ttcttctctt cccggacttg gacttgaaca agacagacaa ggtccagagg agtccgacga
     1381 gttccagagc ggaggttccg ggggtactgg gggtagtgga ggaagtgtga gcaagggcga
     1441 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     1501 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     1561 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     1621 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     1681 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     1741 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     1801 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     1861 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     1921 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     1981 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     2041 caagctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     2101 cgccgccggg atcactctcg gcatggacga gctgtacaag taaagcggcc gcgactctag
     2161 agtcgacctg caggcatgca agcttgatat caagcttatc gataatcaac ctctggatta
     2221 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     2281 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     2341 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     2401 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     2461 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     2521 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     2581 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
     2641 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     2701 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     2761 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacctc gagggaatta
     2821 attcgagctc ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact
     2881 ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc
     2941 tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag ctctctggct
     3001 aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt
     3061 gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt
     3121 ggaaaatctc tagcagcatc tagaattaat tccgtgtatt ctatagtgtc acctaaatcg
     3181 tatgtgtatg atacataagg ttatgtatta attgtagccg cgttctaacg acaatatgta
     3241 caagcctaat tgtgtagcat ctggcttact gaagcagacc ctatcatctc tctcgtaaac
     3301 tgccgtcaga gtcggtttgg ttggacgaac cttctgagtt tctggtaacg ccgtcccgca
     3361 cccggaaatg gtcagcgaac caatcagcag ggtcatcgct agccagatcc tctacgccgg
     3421 acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct atatcgccga
     3481 catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt gtttcggcgt
     3541 gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct tgcatgcacc
     3601 attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct tcctaatgca
     3661 ggagtcgcat aagggagagc gtcgaatggt gcactctcag tacaatctgc tctgatgccg
     3721 catagttaag ccagccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc
     3781 tgctcccggc atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga
     3841 ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg cctcgtgata cgcctatttt
     3901 tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa
     3961 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4021 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4081 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4141 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4201 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     4261 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     4321 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     4381 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     4441 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     4501 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     4561 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     4621 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     4681 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     4741 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     4801 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     4861 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     4921 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     4981 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5041 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5101 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5161 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     5221 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     5281 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     5341 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     5401 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     5461 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     5521 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     5581 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     5641 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     5701 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     5761 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     5821 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     5881 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgt ggaatgtgtg
     5941 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     6001 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6061 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
     6121 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     6181 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     6241 ttttggaggc ctaggctttt gcaaaaagct tggacacaag acaggcttgc gagatatgtt
     6301 tgagaatacc actttatccc gcgtcaggga gaggcagtgc gtaaaaagac gcggactcat
     6361 gtgaaatact ggtttttagt gcgccagatc tctataatct cgcgcaacct attttcccct
     6421 cgaacacttt ttaagccgta gataaacagg ctgggacact tcacatgagc gaaaaataca
     6481 tcgtcacctg ggacatgttg cagatccatg cacgtaaact cgcaagccga ctgatgcctt
     6541 ctgaacaatg gaaaggcatt attgccgtaa gccgtggcgg tctgtaccgg gtgcgttact
     6601 ggcgcgtgaa ctgggtattc gtcatgtcga taccgtttgt atttccagct acgatcacga
     6661 caaccagcgc gagcttaaag tgctgaaacg cgcagaaggc gatggcgaag gcttcatcgt
     6721 tattgatgac ctggtggata ccggtggtac tgcggttgcg attcgtgaaa tgtatccaaa
     6781 agcgcacttt gtcaccatct tcgcaaaacc ggctggtcgt ccgctggttg atgactatgt
     6841 tgttgatatc ccgcaagata cctggattga acagccgtgg gatatgggcg tcgtattcgt
     6901 cccgccaatc tccggtcgct aatcttttca acgcctggca ctgccgggcg ttgttctttt
     6961 taacttcagg cgggttacaa tagtttccag taagtattct ggaggctgca tccatgacac
     7021 aggcaaacct gagcgaaacc ctgttcaaac cccgctttaa acatcctgaa acctcgacgc
     7081 tagtccgccg ctttaatcac ggcgcacaac cgcctgtgca gtcggccctt gatggtaaaa
     7141 ccatccctca ctggtatcgc atgattaacc gtctgatgtg gatctggcgc ggcattgacc
     7201 cacgcgaaat cctcgacgtc caggcacgta ttgtgatgag cgatgccgaa cgtaccgacg
     7261 atgatttata cgatacggtg attggctacc gtggcggcaa ctggatttat gagtgggccc
     7321 cggatctttg tgaaggaacc ttacttctgt ggtgtgacat aattggacaa actacctaca
     7381 gagatttaaa gctctaaggt aaatataaaa tttttaagtg tataatgtgt taaactactg
     7441 attctaattg tttgtgtatt ttagattcca acctatggaa ctgatgaatg ggagcagtgg
     7501 tggaatgcct ttaatgagga aaacctgttt tgctcagaag aaatgccatc tagtgatgat
     7561 gaggctactg ctgactctca acattctact cctccaaaaa agaagagaaa ggtagaagac
     7621 cccaaggact ttccttcaga attgctaagt tttttgagtc atgctgtgtt tagtaataga
     7681 actcttgctt gctttgctat ttacaccaca aaggaaaaag ctgcactgct atacaagaaa
     7741 attatggaaa aatattctgt aacctttata agtaggcata acagttataa tcataacata
     7801 ctgttttttc ttactccaca caggcataga gtgtctgcta ttaataacta tgctcaaaaa
     7861 ttgtgtacct ttagcttttt aatttgtaaa ggggttaata aggaatattt gatgtatagt
     7921 gccttgacta gagatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
     7981 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
     8041 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     8101 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     8161 tcatgtctgg atcaactgga taactcaagc taaccaaaat catcccaaac ttcccacccc
     8221 ataccctatt accactgcca attacctgtg gtttcattta ctctaaacct gtgattcctc
     8281 tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa acttagtagt
     8341 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
     8401 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
     8461 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
     8521 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
     8581 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
     8641 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
     8701 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
     8761 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
     8821 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
     8881 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
     8941 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
     9001 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
     9061 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
     9121 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
     9181 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
     9241 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
     9301 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     9361 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     9421 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     9481 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9541 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9601 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9661 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9721 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9781 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9841 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9901 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     9961 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    10021 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    10081 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10141 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10201 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10261 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
    10321 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccaaa tggcagtatt
    10381 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
    10441 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
    10501 aaattttcgg gtttattaca gggacagcag agatccagtt tggatcgata agcttgatat
    10561 cgaattcag
//
