LOCUS       sequence-469215-       10982 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS) variant: CHARMS-DHF (Designed 
            Helical Filament).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10982)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10982)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-DHF
FEATURES             Location/Qualifiers
     source          1..10982
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     regulatory      217..226
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(1840..1842,1843..2556)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1882..1903)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2143..2162)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     2490..2511
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2617..3205
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2670..2690)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3088..3099)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3208..3224)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3209..3225)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             3315..3548
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3573..3591)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3574..3591)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3881..3900)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(4114..4133)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     4233..4255
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(4293..4311)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        4379..4483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4484..4552,4553..5344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4702..4721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5515..6103
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6004..6023
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6257..6274
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(6344..6364)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6349..6678
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6529..6664
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7812..7877
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             8007..8027
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    8452..8586
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8489..8508)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8543..8562
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8754..9387
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    9434..9559
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    10056..10289
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10474..10518
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10803..10920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcggcca ccatgggcga tgagcgacgg
      241 gagctggaaa aggtcgctgt taaagctata atggcagcaa tgcttggaaa cactgacgaa
      301 gtacgagagc agcttcaacg cgcactggaa atcgccaggg agtcaggcac actgctggct
      361 gtcgtactgg ctctcgaagt tgtggcacgg gttgctatcg aagctgctcg gaaaggaaat
      421 actgatgctg tacgggaggc tcttgaagtt gcactggaaa tagctcgaga atcaggtact
      481 aaggtagccg ttgtgctcgc cctcgaagtt gttgcacgag tcgcaataga ggccgcacga
      541 cggggtaacg tgttggctgt aatcctcgct ctcgaagtag ctcttgagat agcacgcgag
      601 tctggtaccg aagaggccgc cctcctggca gttgaagtag tagtcagagt ctcagatgaa
      661 gctaaaaagc aaggcaatgc agtcgctgtt gcagttgctg aacaggttgc aaaaaaaatt
      721 ctcgaggaat caggaggtag tggcgggtcc gagaagcctt tacaggtggc cagagtggaa
      781 agcagtgtcc cacaaacaaa ggaactggga ggcatcacca cccttgatga ccccctagga
      841 caaacgccgg aacttttcga tgcctttatc tgctactgcc ccaacgatat cgagtttgtg
      901 caggagatga tccggcaact agaacagaca gactatcggc ttaagttgtg tgtgtccgac
      961 cgtgacgtcc tgccgggcac ctgtgtctgg tccattgcca gcgagctaat tgagaaaagg
     1021 tgtcgccgca tggtggtggt tgtttctgac gattatctac agagcaagga atgtgacttc
     1081 cagaccaagt ttgcactcag cctgtctcca ggtgtccaac agaagcgact gattcctatt
     1141 aaatacaagg cgatgaagaa ggactttccc agtatcctgc ggttcatcac tatatgcgac
     1201 tataccaacc cttgcaccaa gtcctggttc tggacccgcc ttgccaaggc tttgtccctg
     1261 cccggaggta gtggcgggtc ccctccttct ccccaagaaa actcctatgt cagctccact
     1321 ggtagggctc actccggagc cgcaccatgg cagccactcg ctgcaccatc aggtgcttca
     1381 gcccaggccg cagagcaact gcaacgggga cccaaccagc cagtcgagtc cgacgaaagt
     1441 cttggagggc tgagcgctgc tctgcggtcc tggcacctta caccaagttg tccccttgat
     1501 cctgctcctc tgcgggaggc tgggtgcccc caaggtgaca cagcagggga atcctcatgg
     1561 ggttcaggac ctgggagccg gccaaccgct gttgaaggcc tggctttggg ctcctctgca
     1621 tcatcatcat cagaacctcc ccagattatc atcaatccag ctcgccagaa aatggttcag
     1681 aaattggctt tgtatgagga tggtgccctg gattccttgc aattgctgtc atcttcttct
     1741 cttcccggac ttggacttga acaagacaga caaggtccag aggagtccga cgagttccag
     1801 agcggaggtt ccgggggtac tgggggtagt ggaggaagtg tgagcaaggg cgaggagctg
     1861 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc
     1921 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
     1981 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc
     2041 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
     2101 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag
     2161 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc
     2221 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc
     2281 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc
     2341 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc
     2401 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccaagctg
     2461 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc
     2521 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagagtcgac
     2581 ctgcaggcat gcaagcttga tatcaagctt atcgataatc aacctctgga ttacaaaatt
     2641 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2701 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2761 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2821 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2881 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2941 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3001 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3061 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3121 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3181 atctcccttt gggccgcctc cccgcatcga taccgtcgac ctcgagggaa ttaattcgag
     3241 ctcggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     3301 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     3361 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3421 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3481 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3541 ctctagcagc atctagaatt aattccgtgt attctatagt gtcacctaaa tcgtatgtgt
     3601 atgatacata aggttatgta ttaattgtag ccgcgttcta acgacaatat gtacaagcct
     3661 aattgtgtag catctggctt actgaagcag accctatcat ctctctcgta aactgccgtc
     3721 agagtcggtt tggttggacg aaccttctga gtttctggta acgccgtccc gcacccggaa
     3781 atggtcagcg aaccaatcag cagggtcatc gctagccaga tcctctacgc cggacgcatc
     3841 gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc cgacatcacc
     3901 gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg
     3961 gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc accattcctt
     4021 gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat gcaggagtcg
     4081 cataagggag agcgtcgaat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     4141 aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     4201 ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     4261 accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     4321 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     4381 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     4441 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     4501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     4561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     4621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     4681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     4741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     4801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     4861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     4921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     4981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     5041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     5101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     5161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     5221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     5281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     5341 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     5401 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     5461 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     5521 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     5581 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     5641 agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa
     5701 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     5761 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     5821 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     5881 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     5941 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     6001 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     6061 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     6121 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     6181 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     6241 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa
     6301 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tgtggaatgt gtgtcagtta
     6361 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     6421 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
     6481 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
     6541 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     6601 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     6661 ggcctaggct tttgcaaaaa gcttggacac aagacaggct tgcgagatat gtttgagaat
     6721 accactttat cccgcgtcag ggagaggcag tgcgtaaaaa gacgcggact catgtgaaat
     6781 actggttttt agtgcgccag atctctataa tctcgcgcaa cctattttcc cctcgaacac
     6841 tttttaagcc gtagataaac aggctgggac acttcacatg agcgaaaaat acatcgtcac
     6901 ctgggacatg ttgcagatcc atgcacgtaa actcgcaagc cgactgatgc cttctgaaca
     6961 atggaaaggc attattgccg taagccgtgg cggtctgtac cgggtgcgtt actggcgcgt
     7021 gaactgggta ttcgtcatgt cgataccgtt tgtatttcca gctacgatca cgacaaccag
     7081 cgcgagctta aagtgctgaa acgcgcagaa ggcgatggcg aaggcttcat cgttattgat
     7141 gacctggtgg ataccggtgg tactgcggtt gcgattcgtg aaatgtatcc aaaagcgcac
     7201 tttgtcacca tcttcgcaaa accggctggt cgtccgctgg ttgatgacta tgttgttgat
     7261 atcccgcaag atacctggat tgaacagccg tgggatatgg gcgtcgtatt cgtcccgcca
     7321 atctccggtc gctaatcttt tcaacgcctg gcactgccgg gcgttgttct ttttaacttc
     7381 aggcgggtta caatagtttc cagtaagtat tctggaggct gcatccatga cacaggcaaa
     7441 cctgagcgaa accctgttca aaccccgctt taaacatcct gaaacctcga cgctagtccg
     7501 ccgctttaat cacggcgcac aaccgcctgt gcagtcggcc cttgatggta aaaccatccc
     7561 tcactggtat cgcatgatta accgtctgat gtggatctgg cgcggcattg acccacgcga
     7621 aatcctcgac gtccaggcac gtattgtgat gagcgatgcc gaacgtaccg acgatgattt
     7681 atacgatacg gtgattggct accgtggcgg caactggatt tatgagtggg ccccggatct
     7741 ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct acagagattt
     7801 aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta ctgattctaa
     7861 ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag tggtggaatg
     7921 cctttaatga ggaaaacctg ttttgctcag aagaaatgcc atctagtgat gatgaggcta
     7981 ctgctgactc tcaacattct actcctccaa aaaagaagag aaaggtagaa gaccccaagg
     8041 actttccttc agaattgcta agttttttga gtcatgctgt gtttagtaat agaactcttg
     8101 cttgctttgc tatttacacc acaaaggaaa aagctgcact gctatacaag aaaattatgg
     8161 aaaaatattc tgtaaccttt ataagtaggc ataacagtta taatcataac atactgtttt
     8221 ttcttactcc acacaggcat agagtgtctg ctattaataa ctatgctcaa aaattgtgta
     8281 cctttagctt tttaatttgt aaaggggtta ataaggaata tttgatgtat agtgccttga
     8341 ctagagatca taatcagcca taccacattt gtagaggttt tacttgcttt aaaaaacctc
     8401 ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgttgt taacttgttt
     8461 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
     8521 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
     8581 tggatcaact ggataactca agctaaccaa aatcatccca aacttcccac cccataccct
     8641 attaccactg ccaattacct gtggtttcat ttactctaaa cctgtgattc ctctgaatta
     8701 ttttcatttt aaagaaattg tatttgttaa atatgtacta caaacttagt agttggaagg
     8761 gctaattcac tcccaaagaa gacaagatat ccttgatctg tggatctacc acacacaagg
     8821 ctacttccct gattagcaga actacacacc agggccaggg gtcagatatc cactgacctt
     8881 tggatggtgc tacaagctag taccagttga gccagataag gtagaagagg ccaataaagg
     8941 agagaacacc agcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga
     9001 agtgttagag tggaggtttg acagccgcct agcatttcat cacgtggccc gagagctgca
     9061 tccggagtac ttcaagaact gctgatatcg agcttgctac aagggacttt ccgctgggga
     9121 ctttccaggg aggcgtggcc tgggcgggac tggggagtgg cgagccctca gatcctgcat
     9181 ataagcagct gctttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
     9241 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     9301 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     9361 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     9421 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     9481 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     9541 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     9601 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     9661 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     9721 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     9781 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     9841 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     9901 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     9961 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
    10021 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
    10081 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
    10141 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
    10201 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
    10261 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
    10321 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
    10381 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
    10441 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
    10501 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
    10561 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
    10621 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
    10681 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
    10741 agatccattc gattagtgaa cggatctcga cggtatcgcc aaatggcagt attcatccac
    10801 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
    10861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
    10921 cgggtttatt acagggacag cagagatcca gtttggatcg ataagcttga tatcgaattc
    10981 ag
//
