LOCUS       sequence-469221-       10419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Chimeric Higher-Order Assemblies for 
            Receptor Mediated Signaling (CHARMS): CHARMS-TIR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10419)
  AUTHORS   Lichtenstein MA, Cao F, Lobnow F, Dirvanskyte P, Kulesza A, Ziska E,
            Taylor MJ
  TITLE     Bottom-up reconstruction of functional Death Domain Signalosomes 
            reveals a requirement for polymer stability and avidity
  JOURNAL   bioRxiv 2024.05.08.593169
REFERENCE   2  (bases 1 to 10419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2024.05.08.593169"
COMMENT     Sequence Label: pHRSV_CHARMS-TIR
FEATURES             Location/Qualifiers
     source          1..10419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..211
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      70..205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             join(1277..1279,1280..1993)
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(1319..1340)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1580..1599)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1927..1948
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    2054..2642
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2107..2127)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2525..2536)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2645..2661)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2646..2662)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2752..2985
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     promoter        complement(3010..3028)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(3011..3028)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(3318..3337)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(3551..3570)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3670..3692
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3730..3748)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3816..3920
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3921..3989,3990..4781)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4139..4158)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4952..5540
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5441..5460
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5694..5711
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(5781..5801)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5786..6115
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5966..6101
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     intron          7249..7314
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             7444..7464
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    7889..8023
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7926..7945)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7980..7999
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             8191..8824
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    8871..8996
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9493..9726
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9911..9955
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10240..10357
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta
       61 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
      121 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag
      181 tagtgaggag gcttttttgg aggcctaggc tacgcgtgcc accatgccac aaacaaagga
      241 actgggaggc atcaccaccc ttgatgaccc cctaggacaa acgccggaac ttttcgatgc
      301 ctttatctgc tactgcccca acgatatcga gtttgtgcag gagatgatcc ggcaactaga
      361 acagacagac tatcggctta agttgtgtgt gtccgaccgt gacgtcctgc cgggcacctg
      421 tgtctggtcc attgccagcg agctaattga gaaaaggtgt cgccgcatgg tggtggttgt
      481 ttctgacgat tatctacaga gcaaggaatg tgacttccag accaagtttg cactcagcct
      541 gtctccaggt gtccaacaga agcgactgat tcctattaaa tacaaggcga tgaagaagga
      601 ctttcccagt atcctgcggt tcatcactat atgcgactat accaaccctt gcaccaagtc
      661 ctggttctgg acccgccttg ccaaggcttt gtccctgccc ggaggtagtg gcgggtcccc
      721 tccttctccc caagaaaact cctatgtcag ctccactggt agggctcact ccggagccgc
      781 accatggcag ccactcgctg caccatcagg tgcttcagcc caggccgcag agcaactgca
      841 acggggaccc aaccagccag tcgagtccga cgaaagtctt ggagggctga gcgctgctct
      901 gcggtcctgg caccttacac caagttgtcc ccttgatcct gctcctctgc gggaggctgg
      961 gtgcccccaa ggtgacacag caggggaatc ctcatggggt tcaggacctg ggagccggcc
     1021 aaccgctgtt gaaggcctgg ctttgggctc ctctgcatca tcatcatcag aacctcccca
     1081 gattatcatc aatccagctc gccagaaaat ggttcagaaa ttggctttgt atgaggatgg
     1141 tgccctggat tccttgcaat tgctgtcatc ttcttctctt cccggacttg gacttgaaca
     1201 agacagacaa ggtccagagg agtccgacga gttccagagc ggaggttccg ggggtactgg
     1261 gggtagtgga ggaagtgtga gcaagggcga ggagctgttc accggggtgg tgcccatcct
     1321 ggtcgagctg gacggcgacg taaacggcca caagttcagc gtgtccggcg agggcgaggg
     1381 cgatgccacc tacggcaagc tgaccctgaa gttcatctgc accaccggca agctgcccgt
     1441 gccctggccc accctcgtga ccaccctgac ctacggcgtg cagtgcttca gccgctaccc
     1501 cgaccacatg aagcagcacg acttcttcaa gtccgccatg cccgaaggct acgtccagga
     1561 gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc cgcgccgagg tgaagttcga
     1621 gggcgacacc ctggtgaacc gcatcgagct gaagggcatc gacttcaagg aggacggcaa
     1681 catcctgggg cacaagctgg agtacaacta caacagccac aacgtctata tcatggccga
     1741 caagcagaag aacggcatca aggtgaactt caagatccgc cacaacatcg aggacggcag
     1801 cgtgcagctc gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct
     1861 gcccgacaac cactacctga gcacccagtc caagctgagc aaagacccca acgagaagcg
     1921 cgatcacatg gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga
     1981 gctgtacaag taaagcggcc gcgactctag agtcgacctg caggcatgca agcttgatat
     2041 caagcttatc gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     2101 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     2161 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     2221 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     2281 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     2341 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     2401 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt
     2461 tccttggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     2521 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     2581 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     2641 gcatcgatac cgtcgacctc gagggaatta attcgagctc ggtaccttta agaccaatga
     2701 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     2761 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     2821 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     2881 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     2941 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagcatc tagaattaat
     3001 tccgtgtatt ctatagtgtc acctaaatcg tatgtgtatg atacataagg ttatgtatta
     3061 attgtagccg cgttctaacg acaatatgta caagcctaat tgtgtagcat ctggcttact
     3121 gaagcagacc ctatcatctc tctcgtaaac tgccgtcaga gtcggtttgg ttggacgaac
     3181 cttctgagtt tctggtaacg ccgtcccgca cccggaaatg gtcagcgaac caatcagcag
     3241 ggtcatcgct agccagatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     3301 aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     3361 cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     3421 actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     3481 caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaatggt
     3541 gcactctcag tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa
     3601 cacccgctga cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg
     3661 tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga
     3721 gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     3781 cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     3841 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     3901 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     3961 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     4021 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     4081 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     4141 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     4201 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     4261 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     4321 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     4381 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     4441 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     4501 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     4561 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     4621 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     4681 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     4741 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     4801 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     4861 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     4921 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     4981 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     5041 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     5101 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     5161 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     5221 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     5281 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     5341 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     5401 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     5461 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     5521 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     5581 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     5641 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     5701 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     5761 cgattcatta atgcagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc
     5821 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa
     5881 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac
     5941 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     6001 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     6061 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct
     6121 tggacacaag acaggcttgc gagatatgtt tgagaatacc actttatccc gcgtcaggga
     6181 gaggcagtgc gtaaaaagac gcggactcat gtgaaatact ggtttttagt gcgccagatc
     6241 tctataatct cgcgcaacct attttcccct cgaacacttt ttaagccgta gataaacagg
     6301 ctgggacact tcacatgagc gaaaaataca tcgtcacctg ggacatgttg cagatccatg
     6361 cacgtaaact cgcaagccga ctgatgcctt ctgaacaatg gaaaggcatt attgccgtaa
     6421 gccgtggcgg tctgtaccgg gtgcgttact ggcgcgtgaa ctgggtattc gtcatgtcga
     6481 taccgtttgt atttccagct acgatcacga caaccagcgc gagcttaaag tgctgaaacg
     6541 cgcagaaggc gatggcgaag gcttcatcgt tattgatgac ctggtggata ccggtggtac
     6601 tgcggttgcg attcgtgaaa tgtatccaaa agcgcacttt gtcaccatct tcgcaaaacc
     6661 ggctggtcgt ccgctggttg atgactatgt tgttgatatc ccgcaagata cctggattga
     6721 acagccgtgg gatatgggcg tcgtattcgt cccgccaatc tccggtcgct aatcttttca
     6781 acgcctggca ctgccgggcg ttgttctttt taacttcagg cgggttacaa tagtttccag
     6841 taagtattct ggaggctgca tccatgacac aggcaaacct gagcgaaacc ctgttcaaac
     6901 cccgctttaa acatcctgaa acctcgacgc tagtccgccg ctttaatcac ggcgcacaac
     6961 cgcctgtgca gtcggccctt gatggtaaaa ccatccctca ctggtatcgc atgattaacc
     7021 gtctgatgtg gatctggcgc ggcattgacc cacgcgaaat cctcgacgtc caggcacgta
     7081 ttgtgatgag cgatgccgaa cgtaccgacg atgatttata cgatacggtg attggctacc
     7141 gtggcggcaa ctggatttat gagtgggccc cggatctttg tgaaggaacc ttacttctgt
     7201 ggtgtgacat aattggacaa actacctaca gagatttaaa gctctaaggt aaatataaaa
     7261 tttttaagtg tataatgtgt taaactactg attctaattg tttgtgtatt ttagattcca
     7321 acctatggaa ctgatgaatg ggagcagtgg tggaatgcct ttaatgagga aaacctgttt
     7381 tgctcagaag aaatgccatc tagtgatgat gaggctactg ctgactctca acattctact
     7441 cctccaaaaa agaagagaaa ggtagaagac cccaaggact ttccttcaga attgctaagt
     7501 tttttgagtc atgctgtgtt tagtaataga actcttgctt gctttgctat ttacaccaca
     7561 aaggaaaaag ctgcactgct atacaagaaa attatggaaa aatattctgt aacctttata
     7621 agtaggcata acagttataa tcataacata ctgttttttc ttactccaca caggcataga
     7681 gtgtctgcta ttaataacta tgctcaaaaa ttgtgtacct ttagcttttt aatttgtaaa
     7741 ggggttaata aggaatattt gatgtatagt gccttgacta gagatcataa tcagccatac
     7801 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
     7861 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
     7921 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     7981 tggtttgtcc aaactcatca atgtatctta tcatgtctgg atcaactgga taactcaagc
     8041 taaccaaaat catcccaaac ttcccacccc ataccctatt accactgcca attacctgtg
     8101 gtttcattta ctctaaacct gtgattcctc tgaattattt tcattttaaa gaaattgtat
     8161 ttgttaaata tgtactacaa acttagtagt tggaagggct aattcactcc caaagaagac
     8221 aagatatcct tgatctgtgg atctaccaca cacaaggcta cttccctgat tagcagaact
     8281 acacaccagg gccaggggtc agatatccac tgacctttgg atggtgctac aagctagtac
     8341 cagttgagcc agataaggta gaagaggcca ataaaggaga gaacaccagc ttgttacacc
     8401 ctgtgagcct gcatgggatg gatgacccgg agagagaagt gttagagtgg aggtttgaca
     8461 gccgcctagc atttcatcac gtggcccgag agctgcatcc ggagtacttc aagaactgct
     8521 gatatcgagc ttgctacaag ggactttccg ctggggactt tccagggagg cgtggcctgg
     8581 gcgggactgg ggagtggcga gccctcagat cctgcatata agcagctgct ttttgcctgt
     8641 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8701 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8761 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8821 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac
     8881 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta
     8941 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta
     9001 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga
     9061 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta
     9121 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat
     9181 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt
     9241 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag
     9301 agcaaaacaa aagtaagacc accgcacagc aagcggccgg ccgctgatct tcagacctgg
     9361 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     9421 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     9481 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     9541 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     9601 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     9661 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     9721 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     9781 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     9841 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     9901 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     9961 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
    10021 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
    10081 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
    10141 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
    10201 atctcgacgg tatcgccaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
    10261 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
    10321 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
    10381 agatccagtt tggatcgata agcttgatat cgaattcag
//
