LOCUS       sequence-469224-        8901 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of mNeonGreen-tagged ARF6 WT.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8901)
  AUTHORS   Nikolatou K, Sandilands E, Roman-Fernandez A, Cumming EM, Freckmann 
            E, Lilla S, Buetow L, McGarry L, Neilson M, Shaw R, Strachan D, 
            Miller C, Huang DT, McNeish IA, Norman JC, Zanivan S, Bryant DM
  TITLE     PTEN deficiency exposes a requirement for an ARF GTPase module for 
            integrin-dependent invasion in ovarian cancer.
  JOURNAL   EMBO J. 2023 Sep 18;42(18):e113987. doi: 10.15252/embj.2023113987. 
            Epub 2023 Aug 14.
  PUBMED    37577760
REFERENCE   2  (bases 1 to 8901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "EMBO J."; 
            date: "2023-09-18"; volume: "42(18):e113987. doi"; pages: " 
            10.15252/embj.2023113987. Epub 2023 Aug 14"
COMMENT     Sequence Label: pLX304 ARF6-R-WT mNG
FEATURES             Location/Qualifiers
     source          1..8901
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        65..368
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        369..572
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     523..543
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     regulatory      601..610
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     regulatory      1147..1156
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1153..1863
                     /codon_start=1
                     /product="bright monomeric yellow-green fluorescent protein
                     derived from LanYFP (Shaner et al., 2013)"
                     /label=mNeonGreen
                     /translation="MVSKGEEDNMASLPATHELHIFGSINGVDFDMVGQGTGNPNDGYE
                     ELNLKSTKGDLQFSPWILVPHIGYGFHQYLPYPDGMSPFQAAMVDGSGYQVHRTMQFED
                     GASLTVNYRYTYEGSHIKGEAQVKGTGFPADGPVMTNSLTAADWCRSKKTYPNDKTIIS
                     TFKWSYTTGNGKRYRSTARTTYTFAKPMAANYLKNQPMYVFRKTELKHSKTELNFKEWQ
                     KAFTDVMGMDELYK"
     regulatory      1408..1417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    1908..2496
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1961..1981)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2379..2390)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2568..2801
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    2879..3000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2916..2935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2970..2989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3040..3175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3195..3214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3196..3214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3224..3241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3224..3240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3233..3255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3382..3837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3469..3488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3679..3700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3863..3967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3968..4036,4037..4828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4186..4205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4999..5587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5488..5507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5741..5758
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    5875..5896
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5911..5916,5917..5934,5935..5941)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5949..5965
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5954..5976
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5973..5989
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5973..5989
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6008..6028
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6010..6028
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6056..6282
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6283..6463
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6510..6635
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7128..7361
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7546..7590
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7765..8265
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     8184..8202
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
     CDS             8277..8672
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    8732..8849
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 cccggggtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
       61 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
      121 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
      181 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
      241 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
      301 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
      361 ttaccatggt gatgcggttt tggcagtaca tcaatgggcg tggatagcgg tttgactcac
      421 ggggatttcc aagtctccac cccattgacg tcaatgggag tttgttttgg caccaaaatc
      481 aacgggactt tccaaaatgt cgtaacaact ccgccccatt gacgcaaatg ggcggtaggc
      541 gtgtacggtg ggaggtctat ataagcagag ctggtttagt gaaccgtcag atccgctagc
      601 gccaccatgg ggaaggtgct atccaaaatc ttcgggaaca aggaaatgcg gatcctcatg
      661 ttgggcctgg acgcggccgg caagacaaca atcctgtaca agttgaagct gggccagtcg
      721 gtgaccacca ttcccactgt gggtttcaac gtggagacgg tgacttacaa aaatgtcaag
      781 ttcaacgtat gggatgtggg cggccaggac aagatccggc cgctctggcg gcattactac
      841 actgggaccc aaggtctcat cttcgtagtg gactgcgccg accgcgaccg catcgatgag
      901 gctcgccagg agctgcaccg cattatcaat gaccgggaga tgagggacgc cataatcctc
      961 atcttcgcca acaagcagga cctgcccgat gccatgaaac cccacgagat ccaggagaaa
     1021 ctgggcctga cccggattcg ggacaggaac tggtatgtgc agccctcctg tgccacctca
     1081 ggggacggac tctatgaggg cctgacctgg ctgaccagca actacaaatc tgaggatcca
     1141 ccggtcgcca ccatggtgag caagggcgag gaggataaca tggcctctct cccagcgaca
     1201 catgagttac acatctttgg ctccatcaac ggtgtggact ttgacatggt gggtcagggc
     1261 accggcaatc caaatgatgg ttatgaggag ttaaacctga agtccaccaa gggtgacctc
     1321 cagttctccc cctggattct ggtccctcat atcgggtatg gcttccatca gtacctgccc
     1381 taccctgacg ggatgtcgcc tttccaggcc gccatggtag atggctccgg ctaccaagtc
     1441 catcgcacaa tgcagtttga agatggtgcc tcccttactg ttaactaccg ctacacctac
     1501 gagggaagcc acatcaaagg agaggcccag gtgaagggga ctggtttccc tgctgacggt
     1561 cctgtgatga ccaactcgct gaccgctgcg gactggtgca ggtcgaagaa gacttacccc
     1621 aacgacaaaa ccatcatcag tacctttaag tggagttaca ccactggaaa tggcaagcgc
     1681 taccggagca ctgcgcggac cacctacacc tttgccaagc caatggcggc taactatctg
     1741 aagaaccagc cgatgtacgt gttccgtaag acggagctca agcactccaa gaccgagctc
     1801 aacttcaagg agtggcaaaa ggcctttacc gatgtgatgg gcatggacga gctgtacaag
     1861 taaagcggcc gcgactctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg
     1921 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     1981 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     2041 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     2101 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     2161 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     2221 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     2281 attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc tgtgttgcca
     2341 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     2401 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     2461 agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac caatgactta
     2521 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2581 tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct ggttagacca
     2641 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
     2701 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
     2761 atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata gtagttcatg
     2821 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     2881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     2941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     3001 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     3061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     3121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     3181 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     3241 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     3301 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     3361 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     3421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     3481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     3541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     3601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     3661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     3721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     3781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     3841 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     3901 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     3961 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4021 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     4081 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     4141 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     4201 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     4261 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     4321 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     4381 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     4441 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     4501 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     4561 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     4621 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     4681 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     4741 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     4801 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     4861 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     4921 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     4981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     5041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     5101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     5161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     5221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     5281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     5341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     5401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     5461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     5521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     5581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     5641 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     5701 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     5761 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     5821 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     5881 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     5941 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     6001 caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag cttaatgtag
     6061 tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca acatgcctta
     6121 caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta cgatcgtgcc
     6181 ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga attgccgcat
     6241 tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct ctggttagac
     6301 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     6361 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     6421 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
     6481 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
     6541 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
     6601 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
     6661 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
     6721 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
     6781 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
     6841 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
     6901 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
     6961 ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag ggacaattgg
     7021 agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt agcacccacc
     7081 aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg agctttgttc
     7141 cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac gctgacggta
     7201 caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct gagggctatt
     7261 gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct ccaggcaaga
     7321 atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg gggttgctct
     7381 ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa taaatctctg
     7441 gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa caattacaca
     7501 agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa
     7561 ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac aaattggctg
     7621 tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag aatagttttt
     7681 gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc gtttcagacc
     7741 cacctcccaa ccccgagggg acccttgcgc cttttccaag gcagccctgg gtttgcgcag
     7801 ggacgcggct gctctgggcg tggttccggg aaacgcagcg gcgccgaccc tgggtctcgc
     7861 acattcttca cgtccgttcg cagcgtcacc cggatcttcg ccgctaccct tgtgggcccc
     7921 ccggcgacgc ttcctgctcc gcccctaagt cgggaaggtt ccttgcggtt cgcggcgtgc
     7981 cggacgtgac aaacggaagc cgcacgtctc actagtaccc tcgcagacgg acagcgccag
     8041 ggagcaatgg cagcgcgccg accgcgatgg gctgtggcca atagcggctg ctcagcaggg
     8101 cgcgccgaga gcagcggccg ggaaggggcg gtgcgggagg cggggtgtgg ggcggtagtg
     8161 tgggccctgt tcctgcccgc gcggtgttcc gcattctgca agcctccgga gcgcacgtcg
     8221 gcagtcggct ccctcgttga ccgaatcacc gacctctctc cccagggggt accaccatgg
     8281 ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct acaatcaaca
     8341 gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc gacggccgca
     8401 tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgtgcagaa ctcgtggtgc
     8461 tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg atcggaaatg
     8521 agaacagggg catcttgagc ccctgcggac ggtgccgaca ggtgcttctc gatctgcatc
     8581 ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt gggattcgtg
     8641 aattgctgcc ctctggttat gtgtgggagg gcctgcagct gcagtagtaa gaattctaga
     8701 tcttgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg attggggggt
     8761 acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact aaagaattac
     8821 aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc agagatccac
     8881 tttggcgccg gctcgagggg g
//
