LOCUS       sequence-469225-        2278 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Linker plasmid (connect pMB.Pos2 to pMB.002).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2278)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2278)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pM.L3456
FEATURES             Location/Qualifiers
     source          1..2278
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(join(515..1306,1307..1375))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1138..1157
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1376..1480)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      1583..2171
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2072..2091
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ccctgaattc gcatctagaa taaatcgcag ccaagtgagt gaatagatga cgcaccacgg
       61 tcagacacgg ccacatcgta tctcacagga gcaagcgcga taggagcact cacacatagt
      121 acggtgatcc gctgactcct ttgcccaaat aagacgtgag cccgtctcag atgcacacac
      181 tggcttaaga tgacaacgac tagagatcta tgtgaggatc ctaactcgag atcgagctga
      241 gcatgagacg gaaatctgct cgtcagtggt gctcacactg acgaatcatg tacagatcat
      301 accgatgact gcctggcgac tcacaactaa gcaagacagc cggaaccagc gccggcgaac
      361 accactgcat atatggcata tcacaacagt ccaactagtg cactgcagta cagcggccgc
      421 gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt tttaaatcaa
      481 tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc agtgaggcac
      541 ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga
      601 taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata ccgcgggacc
      661 cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg gccgagcgca
      721 gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc cgggaagcta
      781 gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct acaggcatcg
      841 tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa cgatcaaggc
      901 gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg
      961 ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca ctgcataatt
     1021 ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac tcaaccaagt
     1081 cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca atacgggata
     1141 ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt tcttcggggc
     1201 gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc actcgtgcac
     1261 ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca aaaacaggaa
     1321 ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata ctcatactct
     1381 tcctttttca atattattga agcatttatc agggttattg tctcatgagc ggatacatat
     1441 ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc cgaaaagtgc
     1501 cacctgtcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     1561 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     1621 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     1681 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     1741 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     1801 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     1861 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     1921 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     1981 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     2041 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     2101 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     2161 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     2221 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt gcggccgc
//
