LOCUS       sequence-469238-        5969 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of AEBP2 [Isoform 3].
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMB.AI3
FEATURES             Location/Qualifiers
     source          1..5969
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  892..1116
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1183..1716)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1905..1910,1911..1927,1928..1933))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2481..2572
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2497..2514
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    3653..3787
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3690..3709)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3744..3763
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  3997..4162
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      4352..4807
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4439..4458)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4649..4670
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4834..4938
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4939..5007,5008..5799)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5157..5176)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcagaccag ccgcgtaacc
      841 tggcaaaatc ggttacggtt gagtaataaa tggatgccct gcgtaagcgg gtgtgggcgg
      901 acaataaagt cttaaactga acaaaataga tctaaactat gacaataaag tcttaaacta
      961 gacagaatag ttgtaaactg aaatcagtcc agttatgctg tgaaaaagca tactggactt
     1021 ttgttatggc taaagcaaac tcttcatttt ctgaagtgca aattgcccgt cgtattaaag
     1081 aggggcgtgg ccaagggcat ggtaaagact atattcgcgg cgttgtgaca atttaccgaa
     1141 caactccgcg gccgggaagc cgatctcggc ttgaacgaat tgttaggtgg cggtacttgg
     1201 gtcgatatca aagtgcatca cttcttcccg tatgcccaac tttgtataga gagccactgc
     1261 gggatcgtca ccgtaatctg cttgcacgta gatcacataa gcaccaagcg cgttggcctc
     1321 atgcttgagg agattgatga gcgcggtggc aatgccctgc ctccggtgct cgccggagac
     1381 tgcgagatca tagatataga tctcactacg cggctgctca aacttgggca gaacgtaagc
     1441 cgcgagagcg ccaacaaccg cttcttggtc gaaggcagca agcgcgatga atgtcttact
     1501 acggagcaag ttcccgaggt aatcggagtc cggctgatgt tgggagtagg tggctacgtc
     1561 gccgaactca cgaccgaaaa gatcaagagc agcccgcatg gatttgactt ggtcagggcc
     1621 gagcctacat gtgcgaatga tgcccatact tgagccacct aactttgttt tagggcgact
     1681 gccctgctgc gtaacatcgt tgctgctgcg taacatcgtt gctgctccat aacatcaaac
     1741 atcgacccac ggcgtaacgc gcttgctgct tggatgcccg aggcatagac tgtacaaaaa
     1801 aacagtcata acaagccatg aaaaccgcca ctgcgccgtt accaccgctg cgttcggtca
     1861 aggttctgga ccagttgcgt gagcgcatac gctacttgca ttacagttta cgaaccgaac
     1921 aggcttatgt caactgggtt cgtgccttca tccgtttcca cggtgtgcgt cacccggcaa
     1981 ccttgggcag cagcgaagtc gaggcatttc tgtcctggct ggcgaacgag cgcaaggttt
     2041 cggactccac gcatcgtcag gcattggcgg ccttgctgtt cttctacggc aaggtgctgt
     2101 gcacggatct gccctggctt caggagatcg gaagacctcg gccgtcgcgg cgcttgccgg
     2161 tggtgctgac cccggatgaa gtggttcgca tcctcggttt tctggaaggc gagcatcgtt
     2221 tgttcgccca ggactctagc tatagttccc tgaattcgca tctagagcct gcactagacg
     2281 aactaggcaa gatgcgtcca atccgtctaa acatggtgac aacgctggac agatgacgta
     2341 acaccgagcc acatcctgaa atcgaggcag gctaaccgaa accgtgacaa tgcaaagaga
     2401 cagcctgacc gtctcaggta gactacccat gagtcacaat gaacgctagt ggttggctac
     2461 atactccgga atattaatag atcatggaga taattaaaat gataaccatc tcgcaaataa
     2521 ataagtattt tactgttttc gtaacagttt tgtaataaaa aaacctataa atattccgga
     2581 ttattcatac cgtcccacca tcgggcgctc cggtatgtac acacgtcggt actcaagcat
     2641 aagcagtact ataatggatg tagacagcac aatttccagt gggcgttcaa ctccagcaat
     2701 gatgaatgga caaggaagca ctacttcttc aagcaaaaat attgcctata attgttgttg
     2761 ggaccagtgc caggcttgct tcaactctag cccagacctg gcagatcaca tccgttccat
     2821 acatgtagat ggtcagcgag gaggggtatt tgtttgctta tggaaaggtt gtaaagtata
     2881 taacactcca tctaccagtc aaagttggtt acaaaggcat atgctgacac acagtggaga
     2941 caaacctttc aagtgtgttg ttggtggctg caatgccagc tttgcttctc agggagggct
     3001 agctcgtcat gtacccacac acttcagtca gcagaactcc tcaaaagttt ctagccagcc
     3061 aaaggccaaa gaagaatctc cttctaaagc tggaatgaac aaaaggagga aattaaagaa
     3121 caaaagacga cgctcattac cacggccaca tgatttcttc gatgcacaaa cactggatgc
     3181 gataagacat cgagccatat gctttaacct ctcagctcat atagaaagtt tagggaaggg
     3241 acacagtgtt gtttttcata gtactgtaat agctaagaga aaagaagatt ctgggaagat
     3301 caaacttttg cttcattgga tgcctgaaga cattctgcct gatgtgtggg tgaatgaaag
     3361 tgaacgacat cagttaaaaa ctaaagtagt tcatttatca aagctaccca aagatactgc
     3421 cttgcttttg gacccaaaca tatacagaac aatgccgcag aagaggttga agagaactct
     3481 gataagaaaa gtgttcaatt tgtatttaag caaacaggga tcctaatgag tcgagaagta
     3541 ctagaggatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt taaaaaacct
     3601 cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg ttaacttgtt
     3661 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
     3721 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
     3781 ctggatctga tcactgatat cgcctaggag atccggggct gaagttgaga cgacgcacaa
     3841 ggtcagggca ctcatgcgac aatcaactcg atgcatgatc cgcaccattg tcgaggggcc
     3901 agcgtcaata gtgccgatga ccacagaccc ggttaagaca tagccgaatg gagccgcgcc
     3961 gaccacagaa tgataactag tgcactgcag tacatcaacc agataagtga aatctagttc
     4021 caaactattt tgtcattttt aattttcgta ttagcttacg acgctacacc cagttcccat
     4081 ctattttgtc actcttccct aaataatcct taaaaactcc atttccaccc ctcccagttc
     4141 ccaactattt tgtccgccca cagcggggca tttttcttcc tgttatgttt ttaatcaaac
     4201 atcctgccaa ctccatgtga caaaccgtca tcttcggcta ctttttctct gtcacagaat
     4261 gaaaattttt ctgtcatctc ttcgttatta atgtttgtaa ttgactgaat atcaacgctt
     4321 atttgcagcc tgaatggcga atggggggag tacgcgccct gtagcggcgc attaagcgcg
     4381 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4441 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4501 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4561 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     4621 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     4681 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     4741 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgttt
     4801 acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     4861 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     4921 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     4981 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     5041 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     5101 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     5161 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     5221 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     5281 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     5341 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     5401 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     5461 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     5521 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     5581 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     5641 gccggtgagc gtggttctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     5701 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     5761 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     5821 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     5881 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     5941 gaccccgtag aaaagatcaa aggatcttc
//
