LOCUS       sequence-469240-        2940 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for RBBP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2940)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 2940)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pCDS.RBBP4
FEATURES             Location/Qualifiers
     source          1..2940
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(1305..1339)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(1410..2069)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     1984..2003
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      2251..2839
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2740..2759
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atggccgaca aggaagcagc cttcgacgac gcagtggaag aacgagtgat
       61 caacgaggaa tacaaaatat ggaaaaagaa cacccctttt ctttatgatt tggtgatgac
      121 ccatgctctg gagtggccca gcctaactgc ccagtggctt ccagatgtaa ccagaccaga
      181 agggaaagat ttcagcattc atcgacttgt cctggggaca cacacatcgg atgaacaaaa
      241 ccatcttgtt atagccagtg tgcagctccc taatgatgat gctcagtttg atgcgtcaca
      301 ctacgacagt gagaaaggag aatttggagg ttttggttca gttagtggaa aaattgaaat
      361 agaaatcaag atcaaccatg aaggagaagt aaacagggcc cgttatatgc cccagaaccc
      421 ttgtatcatc gcaacaaaga ctccttccag tgatgttctt gtttttgact atacaaaaca
      481 tccttctaaa ccagatcctt ctggagagtg caacccagac ttgcgactcc gtggacatca
      541 gaaggaaggc tatgggcttt cttggaaccc aaatctcagt gggcacttac ttagtgcttc
      601 agatgaccat accatctgcc tgtgggacat cagtgccgtt ccaaaggagg gaaaagtggt
      661 agatgcgaag accatcttta cagggcatac ggcagtagta gaagatgttt cctggcatct
      721 actccatgag tctctgtttg ggtcagttgc tgatgatcag aaacttatga tttgggatac
      781 tcgttcaaac aatacttcca aaccaagcca ctcagttgat gctcacactg ctgaagtgaa
      841 ctgcctttct ttcaatcctt atagtgagtt cattcttgcc acaggatcag ctgacaagac
      901 tgttgccttg tgggatctga gaaatctgaa acttaagttg cattcctttg agtcacataa
      961 ggatgaaata ttccaggttc agtggtcacc tcacaatgag actattttag cttccagtgg
     1021 tactgatcgc agactgaatg tctgggattt aagtaaaatt ggagaggaac aatccccaga
     1081 agatgcagaa gacgggccac cagagttgtt gtttattcat ggtggtcata ctgccaagat
     1141 atctgatttc tcctggaatc ccaatgaacc ttgggtgatt tgttctgtat cagaagacaa
     1201 tatcatgcaa gtgtggcaaa tggcagagaa catttataat gatgaagacc ctgaaggaag
     1261 cgttgatcca gaaggacaag ggtccggatc ctgagaccag accaataaaa aacgcccggc
     1321 ggcaaccgag cgttctgaac aaatccagat ggagttctga ggtcattact ggatctatca
     1381 acaggagtcc aagcgagctc gatatcaaat tacgccccgc cctgccactc atcgcagtac
     1441 tgttgtaatt cattaagcat tctgccgaca tggaagccat cacaaacggc atgatgaacc
     1501 tgaatcgcca gcggcatcag caccttgtcg ccttgcgtat aatatttgcc catggtgaaa
     1561 acgggggcga agaagttgtc catattggcc acgtttaaat caaaactggt gaaactcacc
     1621 cagggattgg ctgaaacgaa aaacatattc tcaataaacc ctttagggaa ataggccagg
     1681 ttttcaccgt aacacgccac atcttgcgaa tatatgtgta gaaactgccg gaaatcgtcg
     1741 tggtattcac tccagagcga tgaaaacgtt tcagtttgct catggaaaac ggtgtaacaa
     1801 gggtgaacac tatcccatat caccagctca ccgtctttca ttgccatacg aaattccgga
     1861 tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt gtgcttattt
     1921 ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt ataggtacat
     1981 tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga tatatcaacg
     2041 gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga aaatctcgat
     2101 aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt ggaacctctt
     2161 acgtgcccga tcaatcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     2221 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2281 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     2341 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     2401 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     2461 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     2521 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     2581 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     2641 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     2701 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2761 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2821 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2881 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtag
//
