LOCUS       sequence-469241-        3918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Coding sequence for EZH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3918)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 3918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pCDS.EZH2
FEATURES             Location/Qualifiers
     source          1..3918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      complement(2283..2317)
                     /label=lambda t0 terminator
                     /note="minimal transcription terminator from phage lambda 
                     (Scholtissek and Grosse, 1987)"
     CDS             complement(2388..3047)
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     2962..2981
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     rep_origin      3229..3817
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3718..3737
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tcggtctcat atgggccaga ctgggaagaa atctgagaag ggaccagttt gttggcggaa
       61 gcgtgtaaaa tcagagtaca tgcgactgag acagctcaag aggttcagac gagctgatga
      121 agtaaagagt atgtttagtt ccaatcgtca gaaaattttg gaaagaacgg aaatcttaaa
      181 ccaagaatgg aaacagcgaa ggatacagcc tgtgcacatc ctgacttctg tgagctcatt
      241 gcgcgggact agggagtgtt cggtgaccag tgacttggat tttccaacac aagtcatccc
      301 attaaagact ctgaatgcag ttgcttcagt acccataatg tattcttggt cacccctaca
      361 gcagaatttt atggtggaag atgaaactgt tttacataac attccttata tgggagatga
      421 agttttagat caggatggta ctttcattga agaactaata aaaaattatg atgggaaagt
      481 acacggggat agagaatgtg ggtttataaa tgatgaaatt tttgtggagt tggtgaatgc
      541 ccttggtcaa tataatgatg atgacgatga tgatgatgga gatgatcctg aagaaagaga
      601 agaaaagcag aaagacctgg aggatcaccg agatgataaa gaaagccgcc cacctcggaa
      661 atttccttct gataaaattt ttgaagccat ttcctcaatg tttccagata agggcacagc
      721 agaagaacta aaggaaaaat ataaagaact caccgaacag cagctcccag gcgcacttcc
      781 tcctgaatgt acccccaaca tagatggacc aaatgctaaa tctgttcaga gagagcaaag
      841 cttacactcc tttcatacgc ttttctgtag gcgatgtttt aaatatgact gcttcctaca
      901 tcgtaagtgc aattattctt ttcatgcaac acccaacact tataagcgga agaacacaga
      961 aacagctcta gacaacaaac cttgtggacc acagtgttac cagcatttgg agggagcaaa
     1021 ggagtttgct gctgctctca ccgctgagcg gataaagacc ccaccaaaac gtccaggagg
     1081 ccgcagaaga ggacggcttc ccaataacag tagcaggccc agcaccccca ccattaatgt
     1141 gctggaatca aaggatacag acagtgatag ggaagcaggg actgaaacgg ggggagagaa
     1201 caatgataaa gaagaagaag agaagaaaga tgaaacttcg agctcctctg aagcaaattc
     1261 tcggtgtcaa acaccaataa agatgaagcc aaatattgaa cctcctgaga atgtggagtg
     1321 gagtggtgct gaagcctcaa tgtttagagt cctcattggc acttactatg acaatttctg
     1381 tgccattgct aggttaattg ggaccaaaac atgtagacag gtgtatgagt ttagagtcaa
     1441 agaatctagc atcatagctc cagctcccgc tgaggatgtg gatactcctc caaggaaaaa
     1501 gaagaggaaa caccggttgt gggctgcaca ctgcagaaag atacagctga aaaaggacgg
     1561 ctcctctaac catgtttaca actatcaacc ctgtgatcat ccacggcagc cttgtgacag
     1621 ttcgtgccct tgtgtgatag cacaaaattt ttgtgaaaag ttttgtcaat gtagttcaga
     1681 gtgtcaaaac cgctttccgg gatgccgctg caaagcacag tgcaacacca agcagtgccc
     1741 gtgctacctg gctgtccgag agtgtgaccc tgacctctgt cttacttgtg gagccgctga
     1801 ccattgggac agtaaaaatg tgtcctgcaa gaactgcagt attcagcggg gctccaaaaa
     1861 gcatctattg ctggcaccat ctgacgtggc aggctggggg atttttatca aagatcctgt
     1921 gcagaaaaat gaatttatct cagaatactg tggagagatt atttctcaag atgaagctga
     1981 cagaagaggg aaagtgtatg ataaatacat gtgcagcttt ctgttcaact tgaacaatga
     2041 ttttgtggtg gatgcaaccc gcaagggtaa caaaattcgt tttgcaaatc attcggtaaa
     2101 tccaaactgc tatgcaaaag ttatgatggt taacggtgat cacaggatag gtatttttgc
     2161 caagagagcc atccagactg gcgaagagct gttttttgat tacagataca gccaggctga
     2221 tgccctgaag tatgtcggca tcgaaagaga aatggaaatc cctggatcct gagaccagac
     2281 caataaaaaa cgcccggcgg caaccgagcg ttctgaacaa atccagatgg agttctgagg
     2341 tcattactgg atctatcaac aggagtccaa gcgagctcga tatcaaatta cgccccgccc
     2401 tgccactcat cgcagtactg ttgtaattca ttaagcattc tgccgacatg gaagccatca
     2461 caaacggcat gatgaacctg aatcgccagc ggcatcagca ccttgtcgcc ttgcgtataa
     2521 tatttgccca tggtgaaaac gggggcgaag aagttgtcca tattggccac gtttaaatca
     2581 aaactggtga aactcaccca gggattggct gaaacgaaaa acatattctc aataaaccct
     2641 ttagggaaat aggccaggtt ttcaccgtaa cacgccacat cttgcgaata tatgtgtaga
     2701 aactgccgga aatcgtcgtg gtattcactc cagagcgatg aaaacgtttc agtttgctca
     2761 tggaaaacgg tgtaacaagg gtgaacacta tcccatatca ccagctcacc gtctttcatt
     2821 gccatacgaa attccggatg agcattcatc aggcgggcaa gaatgtgaat aaaggccgga
     2881 taaaacttgt gcttattttt ctttacggtc tttaaaaagg ccgtaatatc cagctgaacg
     2941 gtctggttat aggtacattg agcaactgac tgaaatgcct caaaatgttc tttacgatgc
     3001 cattgggata tatcaacggt ggtatatcca gtgatttttt tctccatttt agcttcctta
     3061 gctcctgaaa atctcgataa ctcaaaaaat acgcccggta gtgatcttat ttcattatgg
     3121 tgaaagttgg aacctcttac gtgcccgatc aatcatgacc aaaatccctt aacgtgagtt
     3181 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
     3241 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
     3301 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
     3361 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
     3421 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
     3481 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
     3541 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
     3601 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga gaaaggcgga
     3661 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
     3721 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
     3781 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
     3841 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
     3901 ttctgtggat aaccgtag
//
