LOCUS       sequence-469242-        2032 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Hs TRAV gene for makeTCR assembly.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2032)
  TITLE     makeTCR collection
REFERENCE   2  (bases 1 to 2032)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: makeTCR_Hs.TRAV25
FEATURES             Location/Qualifiers
     source          1..2032
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(362..378)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(362..378)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     terminator      417..448
                     /label=tonB terminator
                     /note="bidirectional E. coli tonB-P14 transcription 
                     terminator"
     promoter        449..551
                     /label=cat promoter
                     /note="promoter of the E. coli cat gene encoding 
                     chloramphenicol acetyltransferase"
     CDS             552..1211
                     /codon_start=1
                     /gene="cat"
                     /product="chloramphenicol acetyltransferase"
                     /label=CmR
                     /note="confers resistance to chloramphenicol"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     primer_bind     complement(618..637)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
     terminator      1235..1262
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     rep_origin      complement(1274..1861)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(1354..1373)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     terminator      complement(1883..1912)
                     /label=T3Te terminator
                     /note="phage T3 early transcription terminator"
     primer_bind     1985..2002
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     1986..2002
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 cgtctcagat gctactcatc acatcaatgt tggtcttatg gatgcaattg tcacaggtga
       61 atggacaaca ggtaatgcaa attcctcagt accagcatgt acaagaagga gaggacttca
      121 ccacgtactg caattcctca actactttaa gcaatataca gtggtataag caaaggcctg
      181 gtggacatcc cgtttttttg atacagttag tgaagagtgg agaagtgaag aagcagaaaa
      241 gactgacatt tcagtttgga gaagcaaaaa agaacagctc cctgcacatc acagccaccc
      301 agactacaga tgtaggaacc tacttctgtt gagacgcagg gaagatggcc gcgaagaaaa
      361 cgtcatagct gtttcctgac tgaccattta aatcatacct gacctccata gcagaaagtc
      421 aaaagcctcc gaccggaggc ttttgacttg atcggcacgt aagaggttcc aactttcacc
      481 ataatgaaat aagatcacta ccgggcgtat tttttgagtt atcgagattt tcaggagcta
      541 aggaagctaa aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc
      601 atcgtaaaga acattttgag gcatttcagt cagttgctca atgtacctat aaccagaccg
      661 ttcagctgga tattacggcc tttttaaaga ccgtaaagaa aaataagcac aagttttatc
      721 cggcctttat tcacattctt gcccgcctga tgaatgctca tccggaattt cgtatggcaa
      781 tgaaagacgg tgagctggtg atatgggata gtgttcaccc ttgttacacc gttttccatg
      841 agcaaactga aacgttttca tcgctctgga gtgaatacca cgacgatttc cggcagtttc
      901 tacacatata ttcgcaagat gtggcgtgtt acggtgaaaa cctggcctat ttccctaaag
      961 ggtttattga gaatatgttt tttgtctcag ccaatccctg ggtgagtttc accagttttg
     1021 atttaaacgt ggccaatatg gacaacttct tcgcccccgt tttcaccatg ggcaaatatt
     1081 atacgcaagg cgacaaggtg ctgatgccgc tggcgattca ggttcatcat gccgtttgtg
     1141 atggcttcca tgtcggcaga atgcttaatg aattacaaca gtactgcgat gagtggcagg
     1201 gcggggcgta atgagggccc aaatgtaatc acctggctca ccttcgggtg ggcctttctg
     1261 cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgatgct
     1321 caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     1381 gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     1441 tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     1501 aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     1561 ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     1621 cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     1681 tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc
     1741 tgaagccagt tacctcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc
     1801 tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca
     1861 agaagatcct ttgattttct accgaagaaa ggcccacccg tgaaggtgag ccagtgagtt
     1921 gattgcagtc cagttacgct ggagtctgag gctcgtcctg aatgatatca agcttgaatt
     1981 cgtttgtaaa acgacggcca gtgctaaccg tatcgcattg gccataccta ca
//
