LOCUS       sequence-469246-       15248 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of ProtA-EZH2, SUZ12, EED and RBBP4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 15248)
  AUTHORS   Lai Z, Flanigan SF, Boudes M, Davidovich C
  TITLE     Modular cloning of multigene vectors for the baculovirus system and 
            yeast.
  JOURNAL   J Mol Biol. 2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943.
  PUBMED    39814169
REFERENCE   2  (bases 1 to 15248)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2025 Jan 13:168943. doi: 10.1016/j.jmb.2025.168943."
COMMENT     Sequence Label: pMY.PRC2
FEATURES             Location/Qualifiers
     source          1..15248
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        854..1267
                     /gene="S. cerevisiae TEF1"
                     /label=TEF1 promoter
                     /note="promoter for EF-1-alpha"
     promoter        2963..3404
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2963..3080
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     3375..3398
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     CDS             3425..3598
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNDAQAPK"
     CDS             3599..3772
                     /codon_start=1
                     /product="IgG-binding unit of Staphylococcus aureus protein
                     A"
                     /label=ProtA
                     /translation="VDNKFNKEQQNAFYEILHLPNLNEEQRNAFIQSLKDDPSQSANLL
                     AEAKKLNGAQAPK"
     CDS             3812..3832
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     terminator      11556..11743
                     /gene="S. cerevisiae ADH1"
                     /label=ADH1 terminator
                     /note="transcription terminator for the S. cerevisiae 
                     alcohol dehydrogenase 1 (ADH1) gene"
     primer_bind     complement(11611..11635)
                     /label=pHybLex-R
                     /note="ADH terminator, reverse primer"
     CDS             12076..12879
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELL
                     ELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQ
                     YSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGE
                     YTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDD
                     VVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             complement(13521..14336)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(13521..14336)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     14244..14263
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     14328..14347
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     rep_origin      14561..15149
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     15050..15069
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 gcggccgcca atgctaaatt cgagtgaaac acaggaagat cagaaaatcc tcatttcatc
       61 catattaaca ataatttcaa atgtttattt gcattatttg aaactaggca agacaagcaa
      121 cgaaacgttt ttgaaaattt tgagtatttt caataaattt gtagaggact cagatattga
      181 aaaaaagcta cagcaattaa tacttgataa gaagagtatt gagaagggca acggttcatc
      241 atctcatgga tctgcacatg aacaaacacc agagtcaaac gacgttgaaa ttgaggctac
      301 tgcgccaatt gatgacaata cagacgatga taacaaaccg aagttatctg atgtagaaaa
      361 ggattaaaga tgctaagaga tagtgatgat atttcataaa taatgtaatt ctatatatgt
      421 taattacctt ttttgcgagg catatttatg gtgaaggata agttttgacc atcaaagaag
      481 gttaatgtgg ctgtggtttc agggtccata aagcccacat ggataacatt acccctgaat
      541 tcgcatctag actgaactgg ccgataattg cagacgaacg ccttgccaac agggagttct
      601 tcagagacat ggaggctcaa aacgaaatta ttgacagcct agacatcaat agtcatacaa
      661 cagaaagcga ccacccaact ttggctgata atagcgtata aacaatgcat actttgtacg
      721 ttcaaaatac aatgcagtag atatatttat gcatattaca tataatacat atcacatagg
      781 aagcaacagg cgcgttggac ttttaatttt cgaggaccgc gaatccttac atcacaccca
      841 atcccccaca agtgatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac
      901 tcttccagat tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag
      961 catactaaat ttcccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt
     1021 tggaaaagaa aaaagacacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat
     1081 ttttatcacg tttctttttc ttgaaaattt ttttttttga tttttttctc tttcgatgac
     1141 ctcccattga tatttaagtt aataaacggt catcaatttc tcaagtttca gtttcatttt
     1201 tcttgttcta ttacaacttt ttttacttct tgctcattag aaagaaagca tagcaatcta
     1261 atctaagttt taattacaaa agatctatgt ccgagaggga agtgtcgact gcgccggcgg
     1321 gaacagacat gcctgcggcc aagaagcaga agctgagcag tgacgagaac agcaatccag
     1381 acctctctgg agatgagaat gatgacgctg tcagtataga aagtggtaca aacactgaac
     1441 gccctgatac acctacaaac acgccaaatg cacctggaag gaaaagttgg ggaaagggaa
     1501 aatggaagtc aaagaaatgc aaatattctt tcaaatgtgt aaatagtctc aaggaagatc
     1561 ataaccaacc attgtttgga gttcagttta actggcacag taaagaagga gatccattag
     1621 tgtttgcaac tgtaggaagc aacagagtta ccttgtatga atgtcattca caaggagaaa
     1681 tccggttgtt gcaatcttac gtggatgctg atgctgatga aaacttttac acttgtgcat
     1741 ggacctatga tagcaatacg agccatcctc tgctggctgt agctggatct agaggcataa
     1801 ttaggataat aaatcctata acaatgcagt gtataaagca ctatgttggc catggaaatg
     1861 ctatcaatga gctgaaattc catccaagag atccaaatct tctcctgtca gtaagtaaag
     1921 atcatgcttt acgattatgg aatatccaga cggacactct ggtggcaata tttggaggcg
     1981 tagaagggca cagagatgaa gttctaagtg ctgattatga tcttttgggt gaaaaaataa
     2041 tgtcctgtgg tatggatcat tctcttaaac tttggaggat caattcaaag agaatgatga
     2101 atgcaattaa ggaatcttat gattataatc caaataaaac taacaggcca tttatttctc
     2161 agaaaatcca ttttcctgat ttttctacca gagacataca taggaattat gttgattgtg
     2221 tgcgatggtt aggcgatttg atactttcta agtcttgtga aaatgccatt gtgtgctgga
     2281 aacctggcaa gatggaagat gatatagata aaattaaacc cagtgaatct aatgtgacta
     2341 ttcttgggcg atttgattac agccagtgtg acatttggta catgaggttt tctatggatt
     2401 tctggcaaaa gatgcttgca ttgggcaatc aagttggcaa actttatgtt tgggatttag
     2461 aagtagaaga tcctcataaa gccaaatgta caacactgac tcatcataaa tgtggtgctg
     2521 ctattcgaca aaccagtttt agcagggata gcagcattct tatagctgtt tgtgatgatg
     2581 ccagtatttg gcgctgggat cgacttcgag gatcctaact cgagagtgct tttaactaag
     2641 aattattagt cttttctgct tattttttca tcatagttta gaacacttta tattaacgaa
     2701 tagtttatga atctatttag gtttaaaaat tgatacagtt ttataagtta ctttttcaaa
     2761 gactcgtgct gtctattgca taatgcactg gaaggggaaa aaaaaggtgc acacgcgtgg
     2821 ctttttcttg aatttgcagt ttgaaaaatg ctgccaaacc agatgtcaac acagctacaa
     2881 cgccccatta tcttagccta aaaaaacctt ctctttggaa ctttcagtaa tacgcttaac
     2941 tgctcattgc tatattgaag tacggattag aagccgccga gcgggcgaca gccctccgac
     3001 ggatgactct cctccgtgcg tcctcgtcat caccggtcgc gttcctgaaa cgcagatgtg
     3061 cctcgcgccg cactgctccg aacaataaag attctacaat actagctttt atggttatga
     3121 agaggaaaaa ttggcagtaa cctggcccca caaaccttca aattaacgaa tcaaattaac
     3181 aaccatagga tgataatgcg attagttttt tagccttatt tctggggtaa ttaatcagcg
     3241 aagcgatgat ttttgatcta ttaacagata tataaatgga aaagctgcat aaccacttta
     3301 actaatactt tcaacatttt cagtttgtat tacttcttat tcaaatgtca taaaagtatc
     3361 aacaaaaaat tgttaatata cctctatact ttaacgtcaa ggagaaaaaa ctatatcaag
     3421 aatggtggac aacaaattca acaaagaaca acaaaacgcg ttctatgaaa tcttacattt
     3481 acctaactta aacgaagaac aacgaaacgc cttcatccaa agtttaaaag atgacccaag
     3541 ccaaagcgct aaccttttag cagaagctaa aaagctaaat gatgctcagg cgccgaaagt
     3601 agacaacaaa ttcaacaaag aacaacaaaa cgcgttctat gaaatcttac atttacctaa
     3661 cttaaacgaa gaacaacgaa acgccttcat ccaaagttta aaagatgacc caagccaaag
     3721 cgctaacctt ttagcagaag ctaaaaagct aaatggtgct caggcgccga aagagctcaa
     3781 aaccgcggct cttgcgcaac acgatgaagc cgagaatttg tattttcagg gttccggtat
     3841 gggccagact gggaagaaat ctgagaaggg accagtttgt tggcggaagc gtgtaaaatc
     3901 agagtacatg cgactgagac agctcaagag gttcagacga gctgatgaag taaagagtat
     3961 gtttagttcc aatcgtcaga aaattttgga aagaacggaa atcttaaacc aagaatggaa
     4021 acagcgaagg atacagcctg tgcacatcct gacttctgtg agctcattgc gcgggactag
     4081 ggagtgttcg gtgaccagtg acttggattt tccaacacaa gtcatcccat taaagactct
     4141 gaatgcagtt gcttcagtac ccataatgta ttcttggtca cccctacagc agaattttat
     4201 ggtggaagat gaaactgttt tacataacat tccttatatg ggagatgaag ttttagatca
     4261 ggatggtact ttcattgaag aactaataaa aaattatgat gggaaagtac acggggatag
     4321 agaatgtggg tttataaatg atgaaatttt tgtggagttg gtgaatgccc ttggtcaata
     4381 taatgatgat gacgatgatg atgatggaga tgatcctgaa gaaagagaag aaaagcagaa
     4441 agacctggag gatcaccgag atgataaaga aagccgccca cctcggaaat ttccttctga
     4501 taaaattttt gaagccattt cctcaatgtt tccagataag ggcacagcag aagaactaaa
     4561 ggaaaaatat aaagaactca ccgaacagca gctcccaggc gcacttcctc ctgaatgtac
     4621 ccccaacata gatggaccaa atgctaaatc tgttcagaga gagcaaagct tacactcctt
     4681 tcatacgctt ttctgtaggc gatgttttaa atatgactgc ttcctacatc gtaagtgcaa
     4741 ttattctttt catgcaacac ccaacactta taagcggaag aacacagaaa cagctctaga
     4801 caacaaacct tgtggaccac agtgttacca gcatttggag ggagcaaagg agtttgctgc
     4861 tgctctcacc gctgagcgga taaagacccc accaaaacgt ccaggaggcc gcagaagagg
     4921 acggcttccc aataacagta gcaggcccag cacccccacc attaatgtgc tggaatcaaa
     4981 ggatacagac agtgataggg aagcagggac tgaaacgggg ggagagaaca atgataaaga
     5041 agaagaagag aagaaagatg aaacttcgag ctcctctgaa gcaaattctc ggtgtcaaac
     5101 accaataaag atgaagccaa atattgaacc tcctgagaat gtggagtgga gtggtgctga
     5161 agcctcaatg tttagagtcc tcattggcac ttactatgac aatttctgtg ccattgctag
     5221 gttaattggg accaaaacat gtagacaggt gtatgagttt agagtcaaag aatctagcat
     5281 catagctcca gctcccgctg aggatgtgga tactcctcca aggaaaaaga agaggaaaca
     5341 ccggttgtgg gctgcacact gcagaaagat acagctgaaa aaggacggct cctctaacca
     5401 tgtttacaac tatcaaccct gtgatcatcc acggcagcct tgtgacagtt cgtgcccttg
     5461 tgtgatagca caaaattttt gtgaaaagtt ttgtcaatgt agttcagagt gtcaaaaccg
     5521 ctttccggga tgccgctgca aagcacagtg caacaccaag cagtgcccgt gctacctggc
     5581 tgtccgagag tgtgaccctg acctctgtct tacttgtgga gccgctgacc attgggacag
     5641 taaaaatgtg tcctgcaaga actgcagtat tcagcggggc tccaaaaagc atctattgct
     5701 ggcaccatct gacgtggcag gctgggggat ttttatcaaa gatcctgtgc agaaaaatga
     5761 atttatctca gaatactgtg gagagattat ttctcaagat gaagctgaca gaagagggaa
     5821 agtgtatgat aaatacatgt gcagctttct gttcaacttg aacaatgatt ttgtggtgga
     5881 tgcaacccgc aagggtaaca aaattcgttt tgcaaatcat tcggtaaatc caaactgcta
     5941 tgcaaaagtt atgatggtta acggtgatca caggataggt atttttgcca agagagccat
     6001 ccagactggc gaagagctgt tttttgatta cagatacagc caggctgatg ccctgaagta
     6061 tgtcggcatc gaaagagaaa tggaaatccc tggatcctaa ctcgagataa agcaatcttg
     6121 atgaggataa tgattttttt ttgaatatac ataaatacta ccgtttttct gctagatttt
     6181 gtgatgacgt aaataagtac atattacttt ttaagccaag acaagattaa gcattaactt
     6241 tacccttttc tttctaagtt tcaatattag ttatcactgt ttaaaagtta tggcgagaac
     6301 gtcggcggtt aaaatatatt accctgaacg gctggatgca cacactggct taagatgaca
     6361 acgttgatag gtcaagatca atgtaaacaa ttactttgtt atgtagagtt tttttagcta
     6421 cctatattcc accataacat caatcatgcg gttgctggtg tatttaccaa taatgtttaa
     6481 tgtatatata tatatatata tatggggccg tatacttaca tatagtagat gtcaagcgta
     6541 ggcgcttccc ctgccggctg tgagggcgcc ataaccaagg tatctataga ccgccaatca
     6601 gcaaactacc tccgtacatt catgttgcac ccacacattt atacacccag accgcgacaa
     6661 attacccata aggttgtttg tgacggcgtc gtacaagaga acgtgggaac tttttaggct
     6721 caccaaaaaa gaaagaaaaa atacgagttg ctgacagaag cctcaagaaa aaaaaaattc
     6781 ttcttcgact atgctggagg cagagatgat cgagccggta gttaactata tatagctaaa
     6841 ttggttccat caccttcttt tctggtgtcg ctccttctag tgctatttct ggcttttcct
     6901 attttttttt ttccattttt ctttctctct ttctaatata taaattctct tgcattttct
     6961 atttttctct ctatctattc tacttgttta ttcccttcaa ggtttttttt taaggagtac
     7021 ttgtttttag aatatacggt caacgaacta taattaacta aacagatcta tggccgacaa
     7081 ggaagcagcc ttcgacgacg cagtggaaga acgagtgatc aacgaggaat acaaaatatg
     7141 gaaaaagaac accccttttc tttatgattt ggtgatgacc catgctctgg agtggcccag
     7201 cctaactgcc cagtggcttc cagatgtaac cagaccagaa gggaaagatt tcagcattca
     7261 tcgacttgtc ctggggacac acacatcgga tgaacaaaac catcttgtta tagccagtgt
     7321 gcagctccct aatgatgatg ctcagtttga tgcgtcacac tacgacagtg agaaaggaga
     7381 atttggaggt tttggttcag ttagtggaaa aattgaaata gaaatcaaga tcaaccatga
     7441 aggagaagta aacagggccc gttatatgcc ccagaaccct tgtatcatcg caacaaagac
     7501 tccttccagt gatgttcttg tttttgacta tacaaaacat ccttctaaac cagatccttc
     7561 tggagagtgc aacccagact tgcgactccg tggacatcag aaggaaggct atgggctttc
     7621 ttggaaccca aatctcagtg ggcacttact tagtgcttca gatgaccata ccatctgcct
     7681 gtgggacatc agtgccgttc caaaggaggg aaaagtggta gatgcgaaga ccatctttac
     7741 agggcatacg gcagtagtag aagatgtttc ctggcatcta ctccatgagt ctctgtttgg
     7801 gtcagttgct gatgatcaga aacttatgat ttgggatact cgttcaaaca atacttccaa
     7861 accaagccac tcagttgatg ctcacactgc tgaagtgaac tgcctttctt tcaatcctta
     7921 tagtgagttc attcttgcca caggatcagc tgacaagact gttgccttgt gggatctgag
     7981 aaatctgaaa cttaagttgc attcctttga gtcacataag gatgaaatat tccaggttca
     8041 gtggtcacct cacaatgaga ctattttagc ttccagtggt actgatcgca gactgaatgt
     8101 ctgggattta agtaaaattg gagaggaaca atccccagaa gatgcagaag acgggccacc
     8161 agagttgttg tttattcatg gtggtcatac tgccaagata tctgatttct cctggaatcc
     8221 caatgaacct tgggtgattt gttctgtatc agaagacaat atcatgcaag tgtggcaaat
     8281 ggcagagaac atttataatg atgaagaccc tgaaggaagc gttgatccag aaggacaagg
     8341 gtccggatcc taactcgaga ttgaattgaa ttgaaatcga tagatcaatt tttttctttt
     8401 ctctttcccc atcctttacg ctaaaataat agtttatttt attttttgaa tattttttat
     8461 ttatatacgt atatatagac tattatttat cttttaatga ttattaagat ttttattaaa
     8521 aaaaaattcg ctcctctttt aatgccttta tgcagttttt ttttcccatt cgatatttct
     8581 atgtgctggt tctgtatggg cacagacaac ctaaacgcac ccatgaacca cacggttagt
     8641 ccaaaagggg cagttcagat tccagatgcg ggaattagct tgctgccacc ctcacctcac
     8701 taacgctgcg gtgtgcggat acttcatgct atttatagac gcgcgtgtcg gaatcagcac
     8761 gcgcaagaac caaatgggaa aatcggaatg ggtccagaac tgctttgagt gctggctatt
     8821 ggcgtctgat ttccgttttg ggaatccttt gccgcgcgcc cctctcaaaa ctccgcacaa
     8881 gtcccagaaa gcgggaaaga aataaaacgc caccaaaaaa aaaaaaataa aagccaatcc
     8941 tcgaagcgtg ggtggtaggc cctggattat cccgtacaag tatttctcag gagtaaaaaa
     9001 accgtttgtt ttggaatttc ccatttcgcg gccacctacg ccgctatctt tgcaacaact
     9061 atctgcgata actcagcaaa ttttgcatat tcgtgttgca gtattgcgat aatgggagtc
     9121 ttacttccaa cataacggca gaaagaaatg tgagaaaatt ttgcatcctt tgcctccgtt
     9181 caagtatata aagtcggcat gcttgataat ctttctttcc atcctacatt gttctaatta
     9241 ttcttattct cctttattct ttcctaacat accaagaaat taatcttctg tcattcgctt
     9301 aaacactata tcaataaaga tctatggcgc ctcagaagca cggcggtggg ggagggggcg
     9361 gctcggggcc cagcgcgggg tccgggggag gcggcttcgg gggttcggcg gcggtggcgg
     9421 cggcgacggc ttcgggcggc aaatccggcg gcgggagctg tggagggggt ggcagttact
     9481 cggcctcctc ctcctcctcc gcggcggcag cggcgggggc tgcggtgtta ccggtgaaga
     9541 agccgaaaat ggagcacgtc caggctgacc acgagctttt cctccaggcc tttgagaagc
     9601 caacacaaat ctatagattt cttcgaactc ggaatctcat agcaccaata tttttgcaca
     9661 gaactcttac ttacatgtct catcgaaact ccagaacaaa catcaaaagg aaaacattta
     9721 aagttgatga tatgttatca aaagtagaga aaatgaaagg agagcaagaa tctcatagct
     9781 tgtcagctca tttgcagctt acgtttactg gtttcttcca caaaaatgat aagccatcac
     9841 caaactcaga aaatgaacaa aattctgtta ccctggaagt cctgcttgtg aaagtttgcc
     9901 acaaaaaaag aaaggatgta agttgtccaa taaggcaagt tcccacaggt aaaaagcagg
     9961 tgcctttgaa tcctgacctc aatcaaacaa aacccggaaa tttcccgtcc cttgcagttt
    10021 ccagtaatga atttgaacct agtaacagcc atatggtgaa gtcttactcg ttgctattta
    10081 gagtgactcg tccaggaaga agagagttta atggaatgat taatggagaa accaatgaaa
    10141 atattgatgt caatgaagag cttccagcca gaagaaaacg aaatcgtgag gatggggaaa
    10201 agacatttgt tgcacaaatg acagtatttg ataaaaacag gcgcttacag cttttagatg
    10261 gggaatatga agtagccatg caggaaatgg aagaatgtcc aataagcaag aaaagagcaa
    10321 catgggagac tattcttgat gggaagaggc tgcctccatt cgaaacattt tctcagggac
    10381 ctacgttgca gttcactctt cgttggacag gagaaaccaa tgataaatct acggctccta
    10441 ttgccaaacc tcttgccact agaaattcag agagtctcca tcaggaaaac aagcctggtt
    10501 cagttaaacc tactcaaact attgctgtta aagaatcatt gactacagac ctacaaacaa
    10561 gaaaagaaaa ggatactcca aatgaaaacc gacaaaaatt aagaatattt tatcagtttc
    10621 tctataacaa caatacaagg caacaaactg aagcaagaga tgacctgcat tgcccttggt
    10681 gtactctgaa ctgccgcaaa ctttatagtt tactcaagca tcttaaactc tgccatagca
    10741 gatttatctt caactatgtt tatcatccaa aaggtgctag gatagatgtt tctatcaatg
    10801 agtgttatga tggctcctat gcaggaaatc ctcaggatat tcatcgccaa cctggatttg
    10861 cttttagtcg caacggacca gttaagagaa cacctatcac acatattctt gtgtgcaggc
    10921 caaaacgaac aaaagcaagc atgtctgaat ttcttgaatc tgaagatggg gaagtagaac
    10981 agcaaagaac atatagtagt ggccacaatc gtctgtattt ccatagtgat acctgcttac
    11041 ctctccgtcc acaagaaatg gaagtagata gtgaagatga aaaagatcct gaatggctaa
    11101 gagaaaaaac cattacacaa attgaagagt tttctgatgt taatgaagga gagaaagaag
    11161 tgatgaaact ctggaatctc catgtcatga agcatgggtt tattgctgac aatcaaatga
    11221 atcatgcctg tatgctgttt gtagaaaatt atggacagaa aataattaag aagaatttat
    11281 gtcgaaactt catgcttcat ctagtcagca tgcatgactt taatcttatt agcataatgt
    11341 caatagataa agctgttacc aagctccgtg aaatgcagca aaaattagaa aagggggaat
    11401 ctgcttcccc tgcaaacgaa gaaataactg aagaacaaaa tgggacagca aatggattta
    11461 gtgaaattaa ctcaaaagag aaagctttgg aaacagatag tgtctcaggg gtttcaaaac
    11521 agagcaaaaa acaaaaactc ggatcctaac tcgaggcgaa tttcttatga tttatgattt
    11581 ttattattaa ataagttata aaaaaaataa gtgtatacaa attttaaagt gactcttagg
    11641 ttttaaaacg aaaattctta ttcttgagta actctttcct gtaggtcagg ttgctttctc
    11701 aggtatagca tgaggtcgct cttattgacc acacctctac cggcatgccg agcaaatgcc
    11761 tgcaaatcgc tccccatttc gctgggtaga ctacccatga gtcacaatga acgactagag
    11821 atctatgtga ggatcctaac tcgagatcga gctgagcagt tacagagatg ttacgaacca
    11881 ctagtgcact gcagtacacg gtttccttga aatttttttg attcggtaat ctccgaacag
    11941 aaggaagaac gaaggaagga gcacagactt agattggtat atatacgcat atgtagtgtt
    12001 gaagaaacat gaaattgccc agtattctta acccaactgc acagaacaaa aacgtgcagg
    12061 aaacgaagat aaatcatgtc gaaagctaca tataaggaac gtgctgctac tcatcctagt
    12121 cctgttgctg ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca
    12181 ttggatgttc gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt
    12241 tgtttactaa aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag
    12301 ccgctaaagg cattatccgc caagtacaat tttttactct tcgaggacag aaaatttgct
    12361 gacattggta atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg
    12421 gcagacatta cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag
    12481 gcggcagaag aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc
    12541 aagggctccc tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac
    12601 aaagattttg ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac
    12661 gattggttga ttatgacacc cggtgtgggt ttagatgaca agggagatgc attgggtcaa
    12721 cagtatagaa ccgtggatga tgtggtttct acaggatctg acattattat tgttggaaga
    12781 ggactatttg caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc
    12841 tgggaagcat atttgagaag atgcggccag caaaactaaa aaactgtatt ataagtaaat
    12901 gcatgtatac taaactcaca aattagagct tcaatttaat tatatcagtt attacccgag
    12961 tagagcactt gaatccactg ccccgggaat ctcggtcgta atgatttcta taatgacgaa
    13021 aaaaaaaaaa ttggaaagaa aaagcttcat ggcctttata aaaaggaact atccaatacc
    13081 tcgccagaac caagtaacag tattttacgg ggcacaaatc aagaacaata agacaggact
    13141 gtaaagatgg acgcattgaa ctccaaagaa caacaagagt tccaaaaagt agtggaacaa
    13201 aagcaaatga aggatttcat gcgtttgtac tctaatctgg tagaaagatg tttcacagac
    13261 tgtgtcaatg acttcacaac atcaaagcta accaataagg aacaaacatg catcatgaag
    13321 tgctcagaaa agttcttgaa gcatagcgaa cgtgtagggc agcgtttcca agaacaaaac
    13381 gctgccttgg gacaaggctt gggccgataa ggtgtactgg cgtatatata tctaattatg
    13441 tatctctggt gtagcccatt tttagcatgt aaatataaag accgagcggc cgcgtgttac
    13501 aaccaattaa ccaattctga ttagaaaaac tcatcgagca tcaaatgaaa ctgcaattta
    13561 ttcatatcag gattatcaat accatatttt tgaaaaagcc gtttctgtaa tgaaggagaa
    13621 aactcaccga ggcagttcca taggatggca agatcctggt atcggtctgc gattccgact
    13681 cgtccaacat caatacaacc tattaatttc ccctcgtcaa aaataaggtt atcaagtgag
    13741 aaatcaccat gagtgacgac tgaatccggt gagaatggca aaagcttatg catttctttc
    13801 cagacttgtt caacaggcca gccattacgc tcgtcatcaa aatcactcgc atcaaccaaa
    13861 ccgttattca ttcgtgattg cgcctgagcg aggcgaaata cgcgatcgct gttaaaagga
    13921 caattacaaa caggaatcga atgcaaccgg cgcaggaaca ctgccagcgc atcaacaata
    13981 ttttcacctg aatcaggata ttcttctaat acctggaatg ctgttttccc ggggatcgca
    14041 gtggtgagta accatgcatc atcaggagta cggataaaat gcttgatggt cggaagaggc
    14101 ataaattccg tcagccagtt tagtctgacc atctcatctg taacatcatt ggcaacgcta
    14161 cctttgccat gtttcagaaa caactctggc gcatcgggct tcccatacaa tcgatagatt
    14221 gtcgcacctg attgcccgac attatcgcga gcccatttat acccatataa atcagcatcc
    14281 atgttggaat ttaatcgcgg cctggagcaa gacgtttccc gttgaatatg gctcataaca
    14341 ccccttgtat tactgtttat gtaagcagac agttttattg ttcatgatga tatattttta
    14401 tcttgtgcaa tgtaacatca gagattttga gacacaacgt ggctttgttg aataaatcga
    14461 acttttgctg agttgaagga tcagtcatga ccaaaatccc ttaacgtgag ttttcgttcc
    14521 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    14581 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    14641 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    14701 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    14761 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    14821 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    14881 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    14941 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    15001 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    15061 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    15121 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    15181 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
    15241 ataaccgt
//
