LOCUS       sequence-469253-       10447 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iSM (iSpyMac) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10447)
  AUTHORS   Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, 
            Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A,
            Beisel CL, Cantz T
  TITLE     PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY 
            activity.
  JOURNAL   Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 
            10.1093/nar/gkaf782.
  PUBMED    40808297
REFERENCE   2  (bases 1 to 10447)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-08-11"; volume: "53(15):gkaf782. doi"; 
            pages: " 10.1093/nar/gkaf782"
COMMENT     Sequence Label: iSM-IP
FEATURES             Location/Qualifiers
     source          1..10447
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1250..2968
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5384..5404
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5468..6020
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5612..5629)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5839..5858
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6032..6631
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6032..6051)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6528..6548
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6657..7245
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6710..6730)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7128..7139)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7317..7550
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7628..7749
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7665..7684)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7719..7738
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7789..7924
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7944..7963)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7945..7963)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7973..7990)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7973..7989)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8096..8113)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8267..8855)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8347..8366)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9026..9817,9818..9886))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9649..9668
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9887..9991)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10070..10089)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    10223..10294
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10299..10345)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10363..10411
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 ctagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcgt ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgtggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat cagcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg cccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagcac aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcca gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct aaaagccaaa
     1201 agatctttgt cgatcctacc atccactcga cacacccgcc agcggccgca tggacaagaa
     1261 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     1321 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     1381 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
     1441 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     1501 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     1561 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     1621 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     1681 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     1741 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     1801 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     1861 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa agctggaaaa
     1921 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
     1981 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     2041 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     2101 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     2161 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     2221 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     2281 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     2341 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     2401 cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga agcagcggac
     2461 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     2521 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     2581 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     2641 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     2701 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     2761 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     2821 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     2881 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     2941 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     3001 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     3061 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     3121 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     3181 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     3241 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     3301 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     3361 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     3421 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     3481 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     3541 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     3601 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     3661 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     3721 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca
     3781 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg
     3841 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     3901 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga
     3961 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     4021 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     4081 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     4141 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     4201 ccacgacgcc tacctaaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     4261 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     4321 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     4381 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     4441 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg
     4501 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgagatcc agaccgtggg
     4561 acagaatgga ggcctgttcg acgacaaccc caagagcccc ctggaggtga cccccagcaa
     4621 actggtgccc ctgaagaaag agctgaaccc caagaaatac ggcggctacc agaagcccac
     4681 caccgcctac cccgtgctgc tgatcaccga caccaagcag ctgatcccca tcagcgtgat
     4741 gaacaagaaa cagttcgagc agaaccccgt gaagttcctg cgggaccggg gctaccagca
     4801 ggtgggcaag aacgacttca tcaagctgcc caagtacacc ctggtggaca tcggcgacgg
     4861 catcaagcgg ctgtgggcca gcagcaagga gatccacaag ggcaaccagc tggtggtgag
     4921 caagaagagc cagatcctgc tgtaccacgc ccaccacctg gacagcgacc tgagcaacga
     4981 ctacctgcag aaccacaacc agcagttcga cgtgctgttc aacgagatca tcagcttcag
     5041 caaaaagtgc aagctgggca aggagcacat ccagaagatc gagaacgtgt acagcaacaa
     5101 gaagaacagc gccagcatcg aggagctggc cgagagcttt atcaaactgc tcggcttcac
     5161 ccagctgggc gccaccagcc ccttcaactt cctgggcgtg aagctgaacc agaagcagta
     5221 caagggcaag aaggactaca tcctgccctg caccgagggc accctgatca gacagagcat
     5281 caccggactg tacgagacca gagtggatct gagcaagatc ggcgaggata gcggcggaag
     5341 cggaggcagc aagagaaccg ccgatggaag cgagttcgag tctcccaaga agaagaggaa
     5401 agtcggaagc ggataagctg ccaagcttcc gagctctcga attcaaagga ggtacccccc
     5461 ccccctaacg ttactggccg aagccgcttg gaataaggcc ggtgtgcgtt tgtctatatg
     5521 ttattttcca ccatattgcc gtcttttggc aatgtgaggg cccggaaacc tggccctgtc
     5581 ttcttgacga gcattcctag gggtctttcc cctctcgcca aaggaatgca aggtctgttg
     5641 aatgtcgtga aggaagcagt tcctctggaa gcttcttgaa gacaaacaac gtctgtagcg
     5701 accctttgca ggcagcggaa ccccccacct ggcgacaggt gcctctgcgg ccaaaagcca
     5761 cgtgtataag atacacctgc aaaggcggca caaccccagt gccacgttgt gagttggata
     5821 gttgtggaaa gagtcaaatg gctctcctca agcgtattca acaaggggct gaaggatgcc
     5881 cagaaggtac cccattgtat gggatctgat ctggggcctc ggtgcacatg ctttacatgt
     5941 gtttagtcga ggttaaaaaa acgtctaggc cccccgaacc acggggacgt ggttttcctt
     6001 tgaaaaacac gatgataata tggccacaac catgaccgag tacaagccca cggtgcgcct
     6061 cgccacccgc gacgacgtcc ccagggccgt acgcaccctc gccgccgcgt tcgccgacta
     6121 ccccgccacg cgccacaccg tcgatccgga ccgccacatc gagcgggtca ccgagctgca
     6181 agaactcttc ctcacgcgcg tcgggctcga catcggcaag gtgtgggtcg cggacgacgg
     6241 cgccgcggtg gcggtctgga ccacgccgga gagcgtcgaa gcgggggcgg tgttcgccga
     6301 gatcggcccg cgcatggccg agttgagcgg ttcccggctg gccgcgcagc aacagatgga
     6361 aggcctcctg gcgccgcacc ggcccaagga gcccgcgtgg ttcctggcca ccgtcggcgt
     6421 ctcgcccgac caccagggca agggtctggg cagcgccgtc gtgctccccg gagtggaggc
     6481 ggccgagcgc gccggggtgc ccgccttcct ggagacctcc gcgccccgca acctcccctt
     6541 ctacgagcgg ctcggcttca ccgtcaccgc cgacgtcgag gtgcccgaag gaccgcgcac
     6601 ctggtgcatg acccgcaagc ccggtgcctg aaaacccgct gatcagcaaa gtcgacaatc
     6661 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     6721 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     6781 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     6841 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     6901 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     6961 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7021 gcactgacaa ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct
     7081 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7141 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
     7201 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcgtcga ctttaagacc
     7261 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7321 agggctaatt cactcccaac gaagacaaga tctgcttttt gcttgtactg ggtctctctg
     7381 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7441 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7501 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtacgtatag
     7561 tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag
     7621 tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7681 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7741 tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat cccgccccta
     7801 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
     7861 gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg cttttttgga
     7921 ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc tcactggccg
     7981 tcgttttaca ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
     8041 gaatcggcca acgcgcggga agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
     8101 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     8161 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     8221 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     8281 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     8341 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     8401 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     8461 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     8521 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     8581 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     8641 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     8701 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     8761 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     8821 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     8881 ggtctgacac tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     8941 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     9001 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     9061 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     9121 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     9181 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     9241 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     9301 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
     9361 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     9421 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     9481 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     9541 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     9601 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
     9661 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     9721 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     9781 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     9841 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     9901 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     9961 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    10021 tcgacggatc gggagatcga tctcccgatc ccctagggtc gactctcagt acaatctgct
    10081 ctgatgccgc tcgactctgg ctctatcgaa tctccgtcgt ttcgagctta cgcgaacagc
    10141 cgtggcgctc atttgctcgt cgggcatcga atctcgtcag ctatcgtcag cttacctttt
    10201 tggcagcgat cgcggctccc gacatcttgg accattagct ccacaggtat cttcttccct
    10261 ctagtggtca taacagcagc ttcagctacc tctcaattca aaaaacccct caagacccgt
    10321 ttagaggccc caaggggtta tgctatcaat cgttgcgtta cacacacaaa aaaccaacac
    10381 acatccatct tcgatggata gcgattttat tatctaactg ctgatcgagt gtagccagat
    10441 ctcgtga
//
