LOCUS       sequence-469259-       10920 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of NM2A (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10920)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 10920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: HA-mNM2A
FEATURES             Location/Qualifiers
     source          1..10920
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(8782..8808)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      8811..8820
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8872..8891)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8873..8891)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8936..9139)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8965..8985)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9140..9519
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     9691..9710
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9785..9889
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9890..9958,9959..10750)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10108..10127)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggccct attcagctgc
     2881 cttggcatcg gccccatctg ctttaccgtc gacctcctcg tctgagcagt cgccagtgcc
     2941 tttccgaaca attcggcgag tcacgacaaa tggcaggtcc ccacgcctca gtttgttctt
     3001 cagggagctg acctcgcggt tcatagcatc agcggtctct gtggcatctt ccagctcacg
     3061 ctgcagcttc cggcgtgagg cattggcccg ctgggcctcc tcttcagcct cctctagctg
     3121 ccgtttaagc tgcttcaggc gggtggacgc cttgtcagcc tggtccttga actgttccgc
     3181 gttcctccgc tcgtcctcca cctgcagcag cacgtccttc agcttcttct ccgtccggcg
     3241 cacctgcttg gaggctgcct ggcgctcctt ggtctcgttg tccagctgtt cctccagctg
     3301 tgcaattttg gcctccaagg ccgcgatgga ggccttgtat ttggacttga cagcactctc
     3361 catttcctgc agcttggcct tgagctcctt gttctggcgt tccagctgct gtcgcgcatt
     3421 ctcattcttc tgtgcgtggc tgcgttccag gttcaggtcg gtgtttattt ggtcgatctg
     3481 caggttggcc ttcttcagcc gatcgttgat cagctccgtg ttgccctgtt cctcctccag
     3541 ctcctcctcc agcagggcaa tgcgggcctc cagtcgccgc ttctcctcta atgctagggc
     3601 ccctttgcca ctgctgttgg cgatctcatc agccagctcg tcccgttcct gttgggcctg
     3661 acgcttagca cgctcagcag ctgccagttc ctcctgcagc tgaatcatct cggcctccat
     3721 gctcttcagc ttcttctcat tctccttggc ctgcgccagg atctcctccc gggaggcgcg
     3781 cgtgtcgtcc agctcccgca tgcagtcctt catctgggcc tgaagcttcc gcagctgttt
     3841 gatggcctct tcccggttct tattggctgt gtcaatgtgt gcctccaggt ccttcagatc
     3901 catctccagt ttcttgcgtg cggccatggc catggagcgc tgcttcctct catcctccag
     3961 ctccgcctcc atctcccgca cctgtctgac cagctgcttc ttcttctcct cgctctgttc
     4021 atcccggccc tgcagatccc gctcaaactg ggccttcatg gcctgcaggt tcacctccag
     4081 gcggagcttg gcatcctccg tggcctgcag ctcatcctcc agctcctcca gctgggtctt
     4141 catctcctcc acctgctgct ccaaggcccg cttggacttc tccagctcgt ggacactctt
     4201 gcccacgtca tccttggagc tcatgaggtc ctccatctcc gtgcggaact gcttgttgag
     4261 ccgctccagc tctgccttct gctccatggc ctcctcaagc gcccgggcca gtgatagcgc
     4321 ctttgtctcc ttctcacggg cctcagcctc agctcggtca cgctcctctg catacttggc
     4381 cgagatggtc ttctcctcgg ctaggagctg gtcgaacttc ttctgcttct tttccaggtt
     4441 ggagacgctc tgccgctggt ggtccaggtc aaccagcagg tcgtccagct cctgctgcag
     4501 ccgtgtcttg gtcttctcca gcttatcgta ggcagccacc ttctcctcaa gccgctggct
     4561 caggccttcc aagtccttct gaagcctccg cttcgcctcc tctgcagtct ccaggcaccc
     4621 tacaccgtcc tccatcttct tcttcatgtc ggtcacctgg gcatggagcg tggcgatctg
     4681 cttctccaag ttgcgcttgg cctcctcctc ctcctccagc tgctccctga aggagttttt
     4741 ctcatcctcc atctgcttga gcttggtgct caggctcagc ttctgccggt tctcctcctg
     4801 gagcaactcc tgtgtgtcct gaagctggga ctccagcgca gagaagtcct tcgtaagctt
     4861 gctggacttg ctgtcagact ggctaaggag accggtcaca ctgtccagtt caacctgcag
     4921 cttggtgacc ttgtcggcca gttcggttcg cacgcgctct ccctcgctga acttgacctg
     4981 cagttcttgc agctgcgcct ccaccttctt gcgcttgtgc tctgagtcgc ccttgccttg
     5041 cagcagggcc ttcacctcat tggccagctc tccccgctca ttctccaggg tctgcttcgc
     5101 cttctcaagg gtagctttta cccgcttcgt ctgctccaac tgatctgcca gctcctccac
     5161 agcctgtgag tgcttctgcc tcatctcctg gatctgggcc tcatgggtct tggcctcgtc
     5221 ctccagagtc ttcttcagga tgctcacctc ctgctcacgc ttcgacctca gctcctgctg
     5281 ggcagccgtg gagtccagcg tgtcctccag ctctgtcttc agcgcctcca gctcctctcc
     5341 cagatcccgt ttctgcttct cggctttatt cctggaggct cgctccgact ccaggtcctc
     5401 ctggagctca gatatctgag tttccagttc tcggatcttc ttcagggcca tattcttctg
     5461 agcagcttct tcttccactc tggccaaggc agcctgcaac tcctcctcct tcttggccag
     5521 ctgcatcttg agctctgcta tctgcgcctg gagctcagca atctggtcac tgaggtctgt
     5581 ggagtctccc tccagcttgc gacgggtctt ctccaactcc tgcctctgct tctcctccct
     5641 acggaggcgc tcttccaggt cggtgatcat tgcctcgtgc ttgttcttga gcttggccag
     5701 gctcttggac ttctcctcct cttccatgag gttggtagtg aactcagcta ctctgtcttc
     5761 cagcagtttc ttctccttgg ccagtttgca gttctggtcc tccatgatga tctggtcctc
     5821 ctccagtttc ttcagcttgg cctcggtggt caccttctca agctgcagct tctgccgggc
     5881 gctctcctcc tcctccaact gctcctcaag ttcctggatg ttctgctgca tcttcttctt
     5941 ctcggcctgc aggtactggc agcgctcctc ctcctcctcc accctggcct ccaggtcatg
     6001 gcatatctcc tccagctcct gcttcttcgc tgtcagacgg gcccggagct cctcagcctc
     6061 ggcacacagc tctgtctccg cctgcagctg ctcctgaagc tgcagcttct ctgccatgag
     6121 ctgagactgc atcgtctcca tctctgtcag cctgttctct gcagccagat gtttctctcg
     6181 aacctttgtc agttccgcct ccttggctaa cagctcatcc tcatgtctta ttgagttcaa
     6241 caggggcttg accttggtga agagcctcca ccactgccag ttgcgcagcc tgaggtacgc
     6301 agcacagttc ctctgtagga ccttcatggc ggtcagctgt tgctgcctct tggcaaaggc
     6361 cttcctggcc aggtagcccc tgcagcaggc ctggaagcca atgatgacat cggtgatctt
     6421 caggtcccgc tcttcctcca ggtgggcgag cactcctgcc cggaagaaca ctttgctctg
     6481 gccgatgcgg tacaggttgc tgtcaagctc caaggctttg atcatgagca cacacgcttg
     6541 cttgccatcc atgaagccct tcgggatgga gttgggggtg aggatctcat acctctgccg
     6601 gaactcctgg aagaccaccc tgttgggaaa gccctggcgg cagatccgga tgccctcaag
     6661 gacgccattg cagcgcagct ggtccagcac caagtgcggg tccagtttgc cggccttctt
     6721 ctcatggttg ggaatgatgc agcgcacgaa gttggggttg gtattcctca acgtggccat
     6781 gagcttggcc agctgctcct tgtacagctg tccgacagta cggaacatgc ccttccgggt
     6841 cttgaaggca ccaggtagtg ctgtctcgga cattccagcc acttggtcca agccaatgat
     6901 ccgatccaca tccttccaca gctcagagac aaacttgtct gaggactggt gaagcagcgt
     6961 ggcgatgttg tcgttcaagg ggtccatgtt cttcatcagc cactcgtcag ctttatagtc
     7021 caccttgccg gcatagtgga taatgcagaa atcagccttg tccttcagtt gcttgggctt
     7081 ctggaacttg gggtgggtgc cctgctcctg caccaccttc tccacgaagc tcttgtcagt
     7141 ggccttagga aaccagcact cctcatctag cagggccagg atgcctgggg gacccgccgg
     7201 cttctcaatg aggtcgatgc agggctgcag gtccaggccg aagtcgatga agttccactc
     7261 gatgccctct cgctggtact cctcctgctc caggatgaac atggtgtggt tgaacagctg
     7321 ctgcagcttc tcgttggtgt agttgatgca cagctgctcg aaggagttca gatcaaagat
     7381 ttcaaagcca gcgatgtcca gtatcccgat aaatgaggcg ccctgcctct tggtcttgtc
     7441 cagagctttg ttgatgcgaa gcaccagcca gcggaacatc cgctcatagg tagccttggc
     7501 caaggcctca atggcaaagt cagcctgctc tttagtctgc gccttctgca catagtctct
     7561 gcccaccttg atgcgtgggg tgaggatgcc tctggtgaag tcggtcacat tgatccccag
     7621 gaggtgggac accttttgag cagctgtgtt gtccggcatg gacgcctggt cagtgttccg
     7681 ctccttcttg aaggcaatgt tgccaagctg aaggacccca gagatgaccc gcagcaagcc
     7741 catctgctca tcctctggga tacccataat tctcatggcc tccattgtct cctggaacat
     7801 gtccttgtcc tgctgcccag ggatggtgac gtgcccgttg gacaggaagc ggtatttgtt
     7861 gtatggctcc aacaggagat cagtcttcag gtgttctccg gccccagaca gcaggtagta
     7921 gaagatgtgg aaggtccgct cctctttggc ttggcggata gcacgagatt tctccagaag
     7981 ataagtctca atgttggcac caacaatgta gccattgaca tcaaagttga tacgaatgaa
     8041 tttaccgaat cgagaggagt tgtcattctt caccgtcttg gcgtttccaa aggcctctag
     8101 gatagggttg gcctgtagca gctgccgctc caactccccc tggtccttct tgctcttgtg
     8161 tgaggaggcc acatgtgcca ggtactggat gactttcttg gtgttctctg tcttccctgc
     8221 tccagactcc cccgtgcaca ggatggactg atcttcccgg tcctgcatca tgctccggta
     8281 ggcagtatct gtgatggcgt agatgtgggg tggcatctcg tgcctcttct tgcccttgta
     8341 catctccacg atctcctctg agtagatggg caggttctta taagggttga tgaccacaca
     8401 gaacaggcct gaataggtgt agataagccc tgagtagtat cgctccttga ggttgtgcag
     8461 caccgaagct tcgttgaggc acgtgagctc tgccatgtcc tccaccttgg agaacttggg
     8521 tgggttcatc ttctggatgt cgtccttgtt caccttcacc ttcttcccat tctctaccag
     8581 ctctacaatg gcctcttctc ccacctcctc cttgaggctg gctggttcaa agccattctt
     8641 gctggaaggc acccatacca acttcttggc agcccagtca gcttgggcca gcgggttatt
     8701 gatgaagttt ttatccacat agaggtactt gtctgcagcc tgctgagcgc ttccgcttcc
     8761 gccggatgcg ctgcctccac cagcgtagtc tgggacgtcg tatgggtagg ccatggtggc
     8821 gatccgagct cggtaccaag cttaagttta aacgctagcc agcttgggtc tccctatagt
     8881 gagtcgtatt aatttcgata agccagtaag cagtgggttc tctagttagc cagagagctc
     8941 tgcttatata gacctcccac cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt
     9001 tgttacgaca ttttggaaag tcccgttgat tttggtgcca aaacaaactc ccattgacgt
     9061 caatggggtg gagacttgga aatccccgtg agtcaaaccg ctatccacgc ccattgatgt
     9121 actgccaaaa ccgcatcacc atggtaatag cgatgactaa tacgtagatg tactgccaag
     9181 taggaaagtc ccataaggtc atgtactggg cataatgcca ggcgggccat ttaccgtcat
     9241 tgacgtcaat agggggcgta cttggcatat gatacacttg atgtactgcc aagtgggcag
     9301 tttaccgtaa atactccacc cattgacgtc aatggaaagt ccctattggc gttactatgg
     9361 gaacatacgt cattattgac gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc
     9421 catttaccgt aagttatgta acgcggaact ccatatatgg gctatgaact aatgaccccg
     9481 taattgatta ctattaataa ctagtcaata atcaatgtca acgcgtatat ctggcccgta
     9541 catcgcgaag cagcgcaaaa cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca
     9601 agccttgcct tgttgtagct taaattttgc tcgcgcacta ctcagcgacc tccaacacac
     9661 aagcagggag cagatactgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
     9721 accatagggg atcgggagat ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa
     9781 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
     9841 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
     9901 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
     9961 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
    10021 catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt
    10081 tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc
    10141 cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc
    10201 accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc
    10261 cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa
    10321 ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga
    10381 accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat
    10441 ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca
    10501 attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc
    10561 ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat
    10621 tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag
    10681 tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa
    10741 gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca
    10801 tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc
    10861 ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc
//
