LOCUS       sequence-469260-        8912 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of chimeric CGN containing the Rod1 domain from CGNL1
            (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8912)
  AUTHORS   Flinois A, Mean I, Mutero-Maeda A, Guillemot L, Citi S
  TITLE     Paracingulin recruits CAMSAP3 to tight junctions and regulates 
            microtubule and polarized epithelial cell organization.
  JOURNAL   J Cell Sci. 2024 Mar 1;137(5):jcs260745. doi: 10.1242/jcs.260745. 
            Epub 2023 May 15.
  PUBMED    37013686
REFERENCE   2  (bases 1 to 8912)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-03-1"; volume: "137(5):jcs260745. doi"; pages: " 
            10.1242/jcs.260745. Epub 2023 May 15"
COMMENT     Sequence Label: GFP-mCGN-mCGNL1-Rod1-Myc
FEATURES             Location/Qualifiers
     source          1..8912
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     685..704
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        820..867
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             886..1260
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1390..1511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1427..1446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1481..1500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1560..1576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1560..1576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1573..1595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1608..1614,1615..1632,1633..1638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1653..1674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1791..1808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1962..2550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2042..2061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2721..3512,3513..3581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3344..3363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3582..3686)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3761..3780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3952..4331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4332..4535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4486..4506
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4580..4599
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4580..4598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             join(4675..4677,4678..4680,4681..5391)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(4675..4677,4678..4680,4681..5391)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(4720..4741)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4981..5000)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5328..5349
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             8557..8586
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(8637..8654)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8643..8867
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
      841 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
      901 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
      961 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     1021 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     1081 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     1141 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     1201 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     1261 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1381 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1501 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     3721 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     3781 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     3841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     3901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     3961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4321 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4441 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4501 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     4561 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
     4621 taaacttaag cttggtaccg agctcggatc cactagtcca gtgtggtgga attcatggtg
     4681 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     4741 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     4801 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     4861 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     4921 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     4981 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5041 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5101 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5161 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5221 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5281 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5341 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gagcggccgc
     5401 atgatggctg atccccgggg ccccgtagac catggagttc aaattcgatt tattacagag
     5461 ccagagggtg ctactgaaat gggcaccctt cgtcgtagtg ggcggcgccc tgcaagggat
     5521 gcaagagcca gtacctatgg ggtcgctgtg cgtgtgcagg gcatcgccgg gcaaccgttt
     5581 gtggttctta acagtggaga gaaagggact gactcctttg gggtccagat caaagggggc
     5641 aacaaccggg ggtccccagg tgcactgagc tccgattcag agctccccga aaacccctac
     5701 tctcaagtca agggacgtcc tgccacttct cggagcagca catctgatga ggagcccaag
     5761 gaccacttga atggaaaact gatccgttcc cagtcgcaag cctctctgac cgggcttgcc
     5821 tttatgagtc ctagtaatag gagcaccagc ttgctggaac tagcccctaa accgacttcc
     5881 tcgattaaca ccattgacac tgcccccttg tcttcagtgg actcgctcat caacaagttt
     5941 gatagtcaga agggggggca ggtccggggt cgcactgggc gccgcacaag gacacttccc
     6001 cacgaacagc gcaagcggag ccagagtctg gacagcaggc tcccacggga tacacgtgag
     6061 gaacgggagc accagtccgc taaccactgg acccgcggca caaagtatga caaccatgtg
     6121 gacagttcaa agaacccatc gcagaagcag agcccgttca gcagcttcag ccgttcgcgt
     6181 cagactcaag actgggtgct tcagagtttt gaggagacac gggatccagc catggtgcag
     6241 ttcaaatcaa ctccagacct tctcagagac cagagagaga cagcgccacc aggcagtgcg
     6301 gaccatgtga aggccaccat ctatggcatc ctaagggagg gaagctcgga gagtgaggca
     6361 tctgtgagga ggaaagttag tttggtgctg gagcaaatgc agcctctggg gatgagagca
     6421 gcagggagtg cccaaggaag caatcaagct ccgaattctc cctctgaagg caacagtcta
     6481 ttggatcaga agaacaaatt gatcctagaa gtgtctgaac tacagcagca actgcaactg
     6541 gagatgaaga accaacagaa catcaaggag gagcgagaaa gaatgagaga ggatttagaa
     6601 gagctgcgag tccggcacca gagccaagtg gaggagacgg ccaccctgca gagacggctg
     6661 gaggaaagtg aaggcgagct gaggaagagc ttggaagagc tgttccaagt gaagatggag
     6721 cgtgaacagc accagactga gatcagagac ctgcaggacc agctgtcgga aatgcatgat
     6781 gagctagaca gcaccaagcg atccgaggac agggagaagg gagccctgat tgaggagctc
     6841 ctgcaagcca agcaggacct tcaagatctg ctcattgcca aagaggaaca ggaagatctc
     6901 ttgaggaagc gggagcggga gctgactgcc ttgaagggcg ccttgaagga ggaggtttcc
     6961 agccatgacc aggagatgga taagctgaag gaacagtacg atgctgagct gcaggccctc
     7021 agggagagtg tggaggaggc aaccaagaat gttgaggtcc tggccagcag gagcaactcc
     7081 tcagagcaga gccaggcgga ggctgacctg cgtgagaaag tgctgaagga ggagaatgag
     7141 aagctgcagg ggaggattgc ggagctggag cgcagggcag cccagctaca gaggcagatg
     7201 gaggatgtga agggtgacga ggcccaagca aaggagacac tccgaaagtg tgagagtgaa
     7261 gtacagcagc tggaggaagc ccttgtgcat gcccgggagg aggaggggaa cctggcagca
     7321 gccaagcggg ctctggaggt ccggctagat gaggcccagc gggggctggc acgccttggg
     7381 caggaacagc aagcactgaa ccgggcccta gaggaagagg ggaagcagcg ggaggctctc
     7441 cggaggagca aggcagagtt ggaggagcag aagcgtttgc tgaacaggac tgtagaccga
     7501 ctcaacaaag agctggagca gattggggat gactccaagc tagccctgca gcagctccag
     7561 gctcagatgg aagattacaa ggaaaaggcc cggaaggaag tagctgatgc ccagcgtcag
     7621 gccaaggatt gggccagtga ggctgaaaag aattcagggg ggctgagccg gcttcaggat
     7681 gagctgcaaa ggctgcggca agccctgcag acatcccagg ctgagcggga cacagccagg
     7741 ctggacaaag agctgctggc ccagcgactg cagggtctag agcaagaggc tgagaacaag
     7801 aaacgcttcc aggacgacaa ggctcgacag ctgaagagcc tagaggagaa agtctcgcgg
     7861 ctggaagcgg agttagatga agaaaagaac actgtggaac tgttaacaga tcgggtgaac
     7921 cgtggccggg atcaggtgga tcaactgagg acagagctca tgcaggagag atctgctcga
     7981 caggacttgg agtgtgacaa aatctccttg gagagacaga acaaggattt gaagacccgg
     8041 ttggccagtt cagagggctt ccagaaaccc agcgctagcc tctcccagct tgagtcccag
     8101 aatcagctgc tgcaagagcg gctgcaagct gaagagagag agaagacagt tctgcagtcc
     8161 accaatcgca aactagaacg gagggttaag gagctctcca tccagattga tgatgaacgg
     8221 cagcatgtca acgaccagaa ggaccagcta accctgaggg tgaaggcttt gaagcggcag
     8281 gtggatgaag cagaagagga aattgagcgg ctggacagcc tgaggaagaa ggcacagcgg
     8341 gaactggagg aacagcatga ggtcaatgaa cagctccaag cccggatcaa atccttggaa
     8401 aaggatgcct ggcggaaagc ttcccgctca gctgctgagt cagccctcaa acaagagggg
     8461 ctgagctctg atgaggagtt tgacaacgtc tacgacccct catccattgc atcactgctt
     8521 actgagagca acctgcagac tagctcatgt atcgatgaac aaaaactcat ctcagaagag
     8581 gatctgaatt aggcggccgc tcgagtctag agggcccgtt taaacccgct gatcagcctc
     8641 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
     8701 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg
     8761 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga
     8821 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatggctt ctgaggcgga
     8881 aagaaccagc tggggctcta gggggtatcc cc
//
