LOCUS       sequence-469267-       10480 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses iSM_PPL2 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10480)
  AUTHORS   Eggenschwiler R, Hoffmann T, Dmytrenko O, Opitz M, Ackel-Zakour M, 
            Wang P, McCallan SA, Fraguas-Eggenschwiler M, Niemann H, Patronov A,
            Beisel CL, Cantz T
  TITLE     PAM-interacting domain turn-helix 51 motifs can improve Cas9-SpRY 
            activity.
  JOURNAL   Nucleic Acids Res. 2025 Aug 11;53(15):gkaf782. doi: 
            10.1093/nar/gkaf782.
  PUBMED    40808297
REFERENCE   2  (bases 1 to 10480)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res."; date: "2025-08-11"; volume: "53(15):gkaf782. doi"; 
            pages: " 10.1093/nar/gkaf782"
COMMENT     Sequence Label: iSM_PPL2-IP
FEATURES             Location/Qualifiers
     source          1..10480
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             1250..2968
                     /codon_start=1
                     /product="N-terminal portion of Streptococcus pyogenes Cas9
                     (Zetsche et al., 2015)"
                     /label=Cas9(N)
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRKL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLKREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIE"
     CDS             5417..5437
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    5501..6053
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(5645..5662)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     5872..5891
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             6065..6664
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(6065..6084)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     6561..6581
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6690..7278
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6743..6763)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7161..7172)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7350..7583
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7661..7782
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7698..7717)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7752..7771
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7822..7957
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(7977..7996)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7978..7996)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8006..8023)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8006..8022)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8129..8146)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8300..8888)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8380..8399)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9059..9850,9851..9919))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9682..9701
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9920..10024)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10103..10122)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    10256..10327
                     /label=5' beta-globin insulator
                     /note="insulator upstream of the human beta-globin locus 
                     (Farrell et al., 2002)"
     terminator      complement(10332..10378)
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     polyA_signal    10396..10444
                     /note="synthetic polyadenylation signal"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 ctagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcgt ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgtggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat cagcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg cccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagcac aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcca gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgaa aactacccct aaaagccaaa
     1201 agatctttgt cgatcctacc atccactcga cacacccgcc agcggccgca tggacaagaa
     1261 gtacagcatc ggcctggaca tcggcaccaa ctctgtgggc tgggccgtga tcaccgacga
     1321 gtacaaggtg cccagcaaga aattcaaggt gctgggcaac accgaccggc acagcatcaa
     1381 gaagaacctg atcggagccc tgctgttcga cagcggcgaa acagccgagg ccacccggct
     1441 gaagagaacc gccagaagaa gatacaccag acggaagaac cggatctgct atctgcaaga
     1501 gatcttcagc aacgagatgg ccaaggtgga cgacagcttc ttccacagac tggaagagtc
     1561 cttcctggtg gaagaggata agaagcacga gcggcacccc atcttcggca acatcgtgga
     1621 cgaggtggcc taccacgaga agtaccccac catctaccac ctgagaaaga aactggtgga
     1681 cagcaccgac aaggccgacc tgcggctgat ctatctggcc ctggcccaca tgatcaagtt
     1741 ccggggccac ttcctgatcg agggcgacct gaaccccgac aacagcgacg tggacaagct
     1801 gttcatccag ctggtgcaga cctacaacca gctgttcgag gaaaacccca tcaacgccag
     1861 cggcgtggac gccaaggcca tcctgtctgc cagactgagc aagagcagaa agctggaaaa
     1921 tctgatcgcc cagctgcccg gcgagaagaa gaatggcctg ttcggaaacc tgattgccct
     1981 gagcctgggc ctgaccccca acttcaagag caacttcgac ctggccgagg atgccaaact
     2041 gcagctgagc aaggacacct acgacgacga cctggacaac ctgctggccc agatcggcga
     2101 ccagtacgcc gacctgtttc tggccgccaa gaacctgtcc gacgccatcc tgctgagcga
     2161 catcctgaga gtgaacaccg agatcaccaa ggcccccctg agcgcctcta tgatcaagag
     2221 atacgacgag caccaccagg acctgaccct gctgaaagct ctcgtgcggc agcagctgcc
     2281 tgagaagtac aaagagattt tcttcgacca gagcaagaac ggctacgccg gctacattga
     2341 cggcggagcc agccaggaag agttctacaa gttcatcaag cccatcctgg aaaagatgga
     2401 cggcaccgag gaactgctcg tgaagctgaa gagagaggac ctgctgcgga agcagcggac
     2461 cttcgacaac ggcagcatcc cccaccagat ccacctggga gagctgcacg ccattctgcg
     2521 gcggcaggaa gatttttacc cattcctgaa ggacaaccgg gaaaagatcg agaagatcct
     2581 gaccttccgc atcccctact acgtgggccc tctggccagg ggaaacagca gattcgcctg
     2641 gatgaccaga aagagcgagg aaaccatcac cccctggaac ttcgaggaag tggtggacaa
     2701 gggcgcttcc gcccagagct tcatcgagcg gatgaccaac ttcgataaga acctgcccaa
     2761 cgagaaggtg ctgcccaagc acagcctgct gtacgagtac ttcaccgtgt ataacgagct
     2821 gaccaaagtg aaatacgtga ccgagggaat gagaaagccc gccttcctga gcggcgagca
     2881 gaaaaaggcc atcgtggacc tgctgttcaa gaccaaccgg aaagtgaccg tgaagcagct
     2941 gaaagaggac tacttcaaga aaatcgagtg cttcgactcc gtggaaatct ccggcgtgga
     3001 agatcggttc aacgcctccc tgggcacata ccacgatctg ctgaaaatta tcaaggacaa
     3061 ggacttcctg gacaatgagg aaaacgagga cattctggaa gatatcgtgc tgaccctgac
     3121 actgtttgag gacagagaga tgatcgagga acggctgaaa acctatgccc acctgttcga
     3181 cgacaaagtg atgaagcagc tgaagcggcg gagatacacc ggctggggca ggctgagccg
     3241 gaagctgatc aacggcatcc gggacaagca gtccggcaag acaatcctgg atttcctgaa
     3301 gtccgacggc ttcgccaaca gaaacttcat gcagctgatc cacgacgaca gcctgacctt
     3361 taaagaggac atccagaaag cccaggtgtc cggccagggc gatagcctgc acgagcacat
     3421 tgccaatctg gccggcagcc ccgccattaa gaagggcatc ctgcagacag tgaaggtggt
     3481 ggacgagctc gtgaaagtga tgggccggca caagcccgag aacatcgtga tcgaaatggc
     3541 cagagagaac cagaccaccc agaagggaca gaagaacagc cgcgagagaa tgaagcggat
     3601 cgaagagggc atcaaagagc tgggcagcca gatcctgaaa gaacaccccg tggaaaacac
     3661 ccagctgcag aacgagaagc tgtacctgta ctacctgcag aatgggcggg atatgtacgt
     3721 ggaccaggaa ctggacatca accggctgtc cgactacgat gtggaccata tcgtgcctca
     3781 gagctttctg aaggacgact ccatcgacaa caaggtgctg accagaagcg acaagaaccg
     3841 gggcaagagc gacaacgtgc cctccgaaga ggtcgtgaag aagatgaaga actactggcg
     3901 gcagctgctg aacgccaagc tgattaccca gagaaagttc gacaatctga ccaaggccga
     3961 gagaggcggc ctgagcgaac tggataaggc cggcttcatc aagagacagc tggtggaaac
     4021 ccggcagatc acaaagcacg tggcacagat cctggactcc cggatgaaca ctaagtacga
     4081 cgagaatgac aagctgatcc gggaagtgaa agtgatcacc ctgaagtcca agctggtgtc
     4141 cgatttccgg aaggatttcc agttttacaa agtgcgcgag atcaacaact accaccacgc
     4201 ccacgacgcc tacctaaacg ccgtcgtggg aaccgccctg atcaaaaagt accctaagct
     4261 ggaaagcgag ttcgtgtacg gcgactacaa ggtgtacgac gtgcggaaga tgatcgccaa
     4321 gagcgagcag gaaatcggca aggctaccgc caagtacttc ttctacagca acatcatgaa
     4381 ctttttcaag accgagatta ccctggccaa cggcgagatc cggaagcggc ctctgatcga
     4441 gacaaacggc gaaaccgggg agatcgtgtg ggataagggc cgggattttg ccaccgtgcg
     4501 gaaagtgctg agcatgcccc aagtgaatat cgtgaaaaag accgagatcc agaccgtggg
     4561 acagaatgga ggcctgttcg acgacaaccc caagagcccc ctggaggtga cccccagcaa
     4621 actggtgccc ctgaagaaag agctgaaccc caagaaatac ggcggctacc agaagcccac
     4681 caccgcctac cccgtgctgg tggtggccaa agtggaaaag ggcaagtcca agaaactgaa
     4741 gagtgtgaag cagctgatcc ccatcagcgt gatgaacaag aaacagttcg agcagaaccc
     4801 cgtgaagttc ctgcgggacc ggggctacca gcaggtgggc aagaacgact tcatcaagct
     4861 gcccaagtac accctggtgg acatcggcga cggcatcaag cggctgtggg ccagcagcaa
     4921 ggagatccac aagggcaacc agctggtggt gagcaagaag agccagatcc tgctgtacca
     4981 cgcccaccac ctggacagcg acctgagcaa cgactacctg cagaaccaca accagcagtt
     5041 cgacgtgctg ttcaacgaga tcatcagctt cagcaaaaag tgcaagctgg gcaaggagca
     5101 catccagaag atcgagaacg tgtacagcaa caagaagaac agcgccagca tcgaggagct
     5161 ggccgagagc tttatcaaac tgctcggctt cacccagctg ggcgccacca gccccttcaa
     5221 cttcctgggc gtgaagctga accagaagca gtacaagggc aagaaggact acatcctgcc
     5281 ctgcaccgag ggcaccctga tcagacagag catcaccgga ctgtacgaga ccagagtgga
     5341 tctgagcaag atcggcgagg atagcggcgg aagcggaggc agcaagagaa ccgccgatgg
     5401 aagcgagttc gagtctccca agaagaagag gaaagtcgga agcggataag ctgccaagct
     5461 tccgagctct cgaattcaaa ggaggtaccc ccccccccta acgttactgg ccgaagccgc
     5521 ttggaataag gccggtgtgc gtttgtctat atgttatttt ccaccatatt gccgtctttt
     5581 ggcaatgtga gggcccggaa acctggccct gtcttcttga cgagcattcc taggggtctt
     5641 tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg tgaaggaagc agttcctctg
     5701 gaagcttctt gaagacaaac aacgtctgta gcgacccttt gcaggcagcg gaacccccca
     5761 cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat aagatacacc tgcaaaggcg
     5821 gcacaacccc agtgccacgt tgtgagttgg atagttgtgg aaagagtcaa atggctctcc
     5881 tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg taccccattg tatgggatct
     5941 gatctggggc ctcggtgcac atgctttaca tgtgtttagt cgaggttaaa aaaacgtcta
     6001 ggccccccga accacgggga cgtggttttc ctttgaaaaa cacgatgata atatggccac
     6061 aaccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc
     6121 cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc
     6181 ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct
     6241 cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc
     6301 ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag
     6361 cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa
     6421 ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct
     6481 gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt
     6541 cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac
     6601 cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc
     6661 ctgaaaaccc gctgatcagc aaagtcgaca atcaacctct ggattacaaa atttgtgaaa
     6721 gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa
     6781 tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat
     6841 cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt
     6901 gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc
     6961 tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc
     7021 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg
     7081 ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt ctgcgcggga
     7141 cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc
     7201 tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc
     7261 tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag ctgtagatct
     7321 tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc aacgaagaca
     7381 agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag cctgggagct
     7441 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     7501 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     7561 gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta ttattcagta
     7621 tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta ttgcagctta
     7681 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     7741 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggctctagct
     7801 atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
     7861 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
     7921 ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc aattcgccct
     7981 atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acactcactg cccgctttcc
     8041 agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg ggaagaggcg
     8101 gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     8161 ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     8221 gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     8281 aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc
     8341 gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     8401 ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     8461 cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     8521 cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     8581 gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     8641 cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     8701 agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg
     8761 ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     8821 ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     8881 gatctcaaga agatcctttg atcttttcta cggggtctga cactcagtgg aacgaaaact
     8941 cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     9001 attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     9061 accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     9121 ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     9181 gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     9241 agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     9301 ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     9361 ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     9421 gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     9481 ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     9541 tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     9601 tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct
     9661 cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta aaagtgctca
     9721 tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     9781 gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     9841 tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     9901 ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     9961 attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
    10021 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat cgatctcccg
    10081 atcccctagg gtcgactctc agtacaatct gctctgatgc cgctcgactc tggctctatc
    10141 gaatctccgt cgtttcgagc ttacgcgaac agccgtggcg ctcatttgct cgtcgggcat
    10201 cgaatctcgt cagctatcgt cagcttacct ttttggcagc gatcgcggct cccgacatct
    10261 tggaccatta gctccacagg tatcttcttc cctctagtgg tcataacagc agcttcagct
    10321 acctctcaat tcaaaaaacc cctcaagacc cgtttagagg ccccaagggg ttatgctatc
    10381 aatcgttgcg ttacacacac aaaaaaccaa cacacatcca tcttcgatgg atagcgattt
    10441 tattatctaa ctgctgatcg agtgtagcca gatctcgtga
//
