LOCUS       sequence-46927-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Bhakta MS, Segal DJ
  TITLE     The generation of zinc finger proteins by modular assembly.
  JOURNAL   Methods Mol Biol. 2010;649:3-30.
  PUBMED    20680825
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Methods Mol
            Biol."; date: "2010"; volume: "649"; pages: "3-30"
COMMENT     Sequence Label: pPGK-empty
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             37..63
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     regulatory      572..581
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    585..719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(622..641)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     676..695
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(811..831)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 cggtccaact gcacctcggt tctatcgatt gacatgtacc catacgatgt cccagactac
       61 gcgaattctt gaaatgaagg taatggaatt ttttatgaaa gtttatggat atagaggtaa
      121 acatttgggt ggatcaagga aaccggacgg agcaatttat actgtcggat ctcctattga
      181 ttacggtgtg atcgtggata ctaaagctta tagcggaggt tataatctgc caattggcca
      241 agcagatgaa atgcaagatt atgtcgaaga aaatcaaaca cgaaacaaac atatcaaccc
      301 taatgaatgg tggaaagtct atccatcttc tgtaacggaa tttaagtttt tatttgtgag
      361 tggtcacttt aaaggaaact acaaagctca gcttacacga ttaaatcata tcactaattg
      421 taatggagct gttcttagtg tagaagagct tttaattggt ggagaaatga ttaaagccgg
      481 cacattaacc ttagaggaag tgagacggaa atttaataac ggcgagataa acttttaatt
      541 aactaatcta gagtcgacct gcagaagctt ggccgccatg gcccaacttg tttattgcag
      601 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      661 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcg
      721 ggaattaatt cggcgcagca ccatggcctg aaataacctc tgaaagagga acttggttag
      781 gtaccttctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
      841 ccccaggctc cccagcaggc aganttgcaa gctgctccat atcacaca
//
