LOCUS       sequence-469277-        8834 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression  of Xenopus CGN in Insect Cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   D'Atri F, Nadalutti F, Citi S
  TITLE     Evidence for a functional interaction between cingulin and ZO-1 in 
            cultured cells.
  JOURNAL   J Biol Chem. 2002 Aug 2;277(31):27757-64. doi: 
            10.1074/jbc.M203717200. Epub 2002 May 21.
  PUBMED    12023291
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1074/jbc.M203717200"; journalName: "J Biol Chem"; date: 
            "2002-08-2- 2"; volume: "277"; issue: "31"; pages: "27757-64"
COMMENT     Sequence Label: xenCGN-Myc-His
FEATURES             Location/Qualifiers
     source          1..8834
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     mobile_element  894..1118
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7R
                     /note="mini-Tn7 element (right end of the Tn7 transposon)"
     CDS             complement(1185..1718)
                     /codon_start=1
                     /gene="aacC1"
                     /product="gentamycin acetyltransferase"
                     /label=GmR
                     /note="confers resistance to gentamycin"
                     /translation="MLRSSNDVTQQGSRPKTKLGGSSMGIIRTCRLGPDQVKSMRAALD
                     LFGREFGDVATYSQHQPDSDYLGNLLRSKTFIALAAFDQEAVVGALAAYVLPKFEQPRS
                     EIYIYDLAVSGEHRRQGIATALINLLKHEANALGAYVIYVQADYGDDPAVALYTKLGIR
                     EEVMHFDIDPSTAT"
     promoter        complement(join(1888..1893,1894..1910,1911..1916))
                     /gene="intI1 (promoter lies within the coding sequence)"
                     /label=Pc promoter
                     /note="class 1 integron promoter"
     promoter        2268..2359
                     /gene="polh from Autographa californica"
                     /label=polyhedrin promoter
                     /note="promoter for the baculovirus polyhedrin gene"
     primer_bind     2284..2301
                     /label=Polyhedrin-F
                     /note="Polyhedrin promoter, forward primer"
     polyA_signal    6707..6841
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6744..6763)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6798..6817
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     mobile_element  6870..7035
                     /mobile_element_type="transposon:Tn7"
                     /label=Tn7L
                     /note="mini-Tn7 element (left end of the Tn7 transposon)"
     rep_origin      7219..7673
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7306..7325)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7515..7536
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7699..7803
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7804..7872,7873..8664)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8022..8041)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat
      781 gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatagacc agccgcgtaa
      841 cctggcaaaa tcggttacgg ttgagtaata aatggatgcc ctgcgtaagc gggtgtgggc
      901 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
      961 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
     1021 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
     1081 agaggggcgt ggccaagggc atggtaaaga ctatattcgc ggcgttgtga caatttaccg
     1141 aacaactccg cggccgggaa gccgatctcg gcttgaacga attgttaggt ggcggtactt
     1201 gggtcgatat caaagtgcat cacttcttcc cgtatgccca actttgtata gagagccact
     1261 gcgggatcgt caccgtaatc tgcttgcacg tagatcacat aagcaccaag cgcgttggcc
     1321 tcatgcttga ggagattgat gagcgcggtg gcaatgccct gcctccggtg ctcgccggag
     1381 actgcgagat catagatata gatctcacta cgcggctgct caaacttggg cagaacgtaa
     1441 gccgcgagag cgccaacaac cgcttcttgg tcgaaggcag caagcgcgat gaatgtctta
     1501 ctacggagca agttcccgag gtaatcggag tccggctgat gttgggagta ggtggctacg
     1561 tctccgaact cacgaccgaa aagatcaaga gcagcccgca tggatttgac ttggtcaggg
     1621 ccgagcctac atgtgcgaat gatgcccata cttgagccac ctaactttgt tttagggcga
     1681 ctgccctgct gcgtaacatc gttgctgctg cgtaacatca aacatcgacc cacggcgtaa
     1741 cgcgcttgct gcttggatgc ccgaggcata gactgtacaa aaaaacagtc ataacaagcc
     1801 atgaaaaccg ccactgcgcc gttaccaccg ctgcgttcgg tcaaggttct ggaccagttg
     1861 cgtgagcgca tacgctactt gcattacagt ttacgaaccg aacaggctta tgtcaactgg
     1921 gttcgtgcct tcatccgttt ccacggtgtg cgtcacccgg caaccttggg cagcagcgaa
     1981 gtcgaggcat ttctgtcctg gctggcgaac gagcgcaagg tttcggtctc cacgcatcgt
     2041 caggcattgg cggccttgct gttcttctac ggcaaggtgc tgtgcacgga tctgccctgg
     2101 cttcaggaga tcggaagacc tcggccgtcg cggcgcttgc cggtggtgct gaccccggat
     2161 gaagtggttc gcatcctcgg ttttctggaa ggcgagcatc gtttgttcgc ccaggactct
     2221 agctatagtt ctagtggttg gctacgtata ctccggaata ttaatagatc atggagataa
     2281 ttaaaatgat aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt
     2341 aataaaaaaa cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc
     2401 ccggtccgaa gcgcgcggaa ttcaaaggcc tacgtcgact aatggaaagg gatatatatg
     2461 gcgacatggc tgaccagcat atccctgtag gtcagggtgt ccagatccgg tttattggtg
     2521 acctgaagga aaatggaaaa cccaggggca agcgatccaa gcaagattcc tacggggtag
     2581 ccgttcgggt gcaagggata gacgggcagc cctttgtggt gttaaatagt ggggacaaag
     2641 ccaagtcttc ttttggagta cagatcaaat cacaggaacc ttatttaaat gcttccaata
     2701 caagcccacc caactaccag aactactcat caaagcccag agggcccagc agatccataa
     2761 gctcagaatc cgaactgcca gagaacccat atggaagcag aggctacaga ccaagcagtt
     2821 cccattacag cagtgcctcc gatgaagagc agaaaccacg tggtaacatc agaggaagcg
     2881 atggattgtc ctctttacct cgacccttgc aggcatcccg tagggaagaa ctcagacgct
     2941 cccagtcgca cagttccttg cttgaacctg atgtcgagga gtcctacgac tatgatcacc
     3001 attatagcga gcgctcatcc acccttgaca ccacttactc ccagtcctcc agagactcgg
     3061 catggagccg cagcagccag aagaagatag acaatggtga ttacccatca cttggctaca
     3121 ggtctgcaac ctctcagcaa tctacctccg tcagtaacaa gacaaagaaa aatggacttt
     3181 ccacctcctc accctcaaat cagtccaacg aggacattga caccaaaccc ctgtcatctg
     3241 tggattcttt gatcaacaag tttgacatta agggccaggt acgaggcagg acggcaagac
     3301 ggagccaggc tctgaaggat gaacggaagc gctctcaaag cttggatggg cgcaagaatt
     3361 accatgacac tgctgactcc agagaaataa ttgttgagaa gcaaaatgag gtgcagacaa
     3421 tgagggaacc tgtcaatgct agtaaccggt cctttaacag acagaccctg gaaagggggg
     3481 atatcagcaa gaccagactg accaaagagt ggttggacca agacagagaa gagccggtga
     3541 ttctgaaaca gcaaagaact gtccagagtg aattccagct gaaaagtacc cagatcttct
     3601 gagggatcag cagccggatg gcagtgaccc cactcgagag atgatctttg gcatccttag
     3661 ggaaggctca ctagagagtg agaataccct gaggaaaaag accagtatcc tgctggagaa
     3721 gttgccttct ttacaagtcc aaccaggaga ggacaccatc tcccttggat cccagaagaa
     3781 agaactggag cggaaagtgg cagaactgca gaggcaactg gatgatgaga tgaagcaaag
     3841 gatgaagttg gaaacctctc aggggcgccc caaggccggc atgcagaggc tggagattga
     3901 gctagaggag agcaaggagg agtgcagccg gctgaaggaa ctgtacgaaa agaagaagaa
     3961 cgagctgagc gcgatgtctc aggagctcat ggaagtgaga atgggcaaag agcaggtgga
     4021 gaccaagctc agaaccatgg aggataaact tatggattcc aaggaagagt tgtcccacct
     4081 acgggcaaag ggtggcacct cccctgacaa actggcttta ttgaaggagc tggaggaggt
     4141 ccaagacgag ctggacgagg tcctacagat ccgccagaag caggaagagc tgttgcgcca
     4201 aaaggaccgg gagctgactg ccctgaaggg ggcgctgaaa gatgaggtgg ccaatcatga
     4261 caaagacttg gaccgggtga gggagcagta ccagaatgac atgcagcagc tgcgaaagaa
     4321 catggacaat gtctctcagg accaactcag cctggagtca gaacggcaga aaataaacca
     4381 ggtggtgcgg aacctgcagc gcgagctgga agagagcagc gacgagatca gccaatggaa
     4441 agagatgttc cagaagaaca aggaggagct gcgcagcaca aagcaggagc tcttgcagat
     4501 gaagctggag aaagaggagt ctgaggatga gctgaaagag acccgagacc gcttctcctt
     4561 actgcagtca gagctggcac aagtcaagaa gggatctgta gatcctggag aagttgccag
     4621 tgttagaaag gagctgcagc gggttcaaga tcagctgaaa caactatctg tggacaagca
     4681 gaaggtggaa gagaacctgc aacagcgaga gagggaaatg tctgccctga aagggacact
     4741 taaggaggag gtatcagggc gggaccgtga gacagtcaga ctgcgggagc agttgcagtc
     4801 agaggtgatg caggtcaaga aagagaatga aggtcttgcc aaggaatctc ggcgcattca
     4861 ggatcagctg aaacaggtgt tgctggaaaa gcagagacat gaggagactg tacaccagag
     4921 agagagggag ctgtcagtgc tgaagggggc cctgaaggac gaggtgtctg gccgagaccg
     4981 tgagacagag aaactgaggg agcggttaga gcaggatgcg ctaatgacta agagaagcta
     5041 cgaagagctg gtcaagatca acaaaaggct ggagagtgag aagacagact tagaacgtgt
     5101 gaggcaagtg atcgagaaca atttgcagga gtcccgcgaa gagaatgacg atctcaggag
     5161 gaagatcttg gggttggagg cacagctgaa ggaaaccaac acattctgcg atgaccttca
     5221 gcgggcagag tctcggctga aagacaagat caataagctg gaggctgaac gcaagagaat
     5281 ggaggacagt ctgggtgaag ttgcggatca ggagcaggaa ttggcttttg tgaaacgtga
     5341 cctggagagc aaactggatg aggctcagcg tagcctgaaa cgtctctctc tggaatatga
     5401 ggagctgcag gagtgctacc aggaggagat gaagcagaag gaccatctta agaaaaccaa
     5461 aaatgagttg gaggagcaga agcgacttct ggacaagtcc atggataaac taaccagaga
     5521 gttggataac atgtccaatg agtcgcgggg atccctgcag ttgctgcaga cccagctgga
     5581 agaatacagg gagaaatctc ggaaagagat tggagaggcc cagaaacagg ctaaggagaa
     5641 aacagccgag gctgagagac accagttcaa ctccagccgg atgcaggaag aggtacagaa
     5701 gctgaaattg gctctacaag agctccaggt ggagaaggag acggtggagc tggacaaaca
     5761 gatgatcagc cagcggctac agagcctgga gcaggacata gagtccaaga aacgagtcca
     5821 ggatgaccgc tcacgacaag tcaaagttct ggaggacaag ctgaagcgca tggaggctga
     5881 gctggacgag gagaagaaca cggtggagct tctgactgac agagttaata ggagcagaga
     5941 ccagatggag cagcagcgag ctgaactgaa ccaggagaga tcccgggggc aggatctgga
     6001 gtgtgacaag atttctctgg agagacagaa caaagagctg aaaaaccgct tggccagcat
     6061 ggaggggcag cagaaaccca gcgtcaatgt gtcccacctg gaggcaaaac tacaggagat
     6121 tcaggagcgg ctgcagttgg aggagaggga aaaggccacc ctactgtcta caaaccgcaa
     6181 actggagagg aaattaaagg aactgaatat ccagttagag gatgagaggc tccaagttaa
     6241 tgaccagaaa gaccagttga acctgagggt taaagccctg aagcggcagg tggatgaggc
     6301 agaggaagag attgagcggc tggagggtct ccggaagaaa gccgtgcgtg agatggagga
     6361 gcagcaggag atcaatgagc agctccagac ccgggttaaa gttatggaga aggaatccaa
     6421 gcgcaagccc atccgaccag cccacgatga cgacctgagc tcagacggag agtttggggg
     6481 cccctacgac cccagttcca tcacctcact gctcacggag agtaacctgc agaccagctc
     6541 atgctctaga gcctgcagtc tcgaggcatg cggtaccaag cttgtcgaga agtactagag
     6601 gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa acctcccaca
     6661 cctccccctg aacctgaaac ataaaatgaa tgcaattgtt gttgttaact tgtttattgc
     6721 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     6781 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     6841 ctgatcactg cttgagccta ggagatccga accagataag tgaaatctag ttccaaacta
     6901 ttttgtcatt tttaattttc gtattagctt acgacgctac acccagttcc catctatttt
     6961 gtcactcttc cctaaataat ccttaaaaac tccatttcca cccctcccag ttcccaacta
     7021 ttttgtccgc ccacagcggg gcatttttct tcctgttatg tttttaatca aacatcctgc
     7081 caactccatg tgacaaaccg tcatcttcgg ctactttttc tctgtcacag aatgaaaatt
     7141 tttctgtcat ctcttcgtta ttaatgtttg taattgactg aatatcaacg cttatttgca
     7201 gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg
     7261 ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct
     7321 tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc
     7381 ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg
     7441 atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt
     7501 ccacgttctt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     7561 ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     7621 gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc
     7681 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     7741 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     7801 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     7861 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     7921 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     7981 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8041 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8101 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8161 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     8221 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     8281 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     8341 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     8401 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     8461 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     8521 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     8581 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     8641 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     8701 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     8761 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     8821 atcaaaggat cttc
//
