LOCUS       sequence-469278-       12829 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Study the role and mechanism of the 3' UTR in regulating RPS6KA3-P3 
            gene function..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12829)
  TITLE     Studying the role and mechanism of the 3' UTR in regulating gene 
            function. Batch 3.
REFERENCE   2  (bases 1 to 12829)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: RPS6KA3-P3
FEATURES             Location/Qualifiers
     source          1..12829
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(747..1538,1539..1607))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     1370..1389
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(1608..1712)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    1868..1916
                     /note="synthetic polyadenylation signal"
     CDS             complement(1980..2771)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(2091..2110)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2701..2720
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     CDS             complement(2823..3755)
                     /codon_start=1
                     /product="Renilla luciferase"
                     /label=hRluc
                     /note="human codon-optimized"
                     /translation="MASKVYDPEQRKRMITGPQWWARCKQMNVLDSFINYYDSEKHAEN
                     AVIFLHGNAASSYLWRHVVPHIEPVARCIIPDLIGMGKSGKSGNGSYRLLDHYKYLTAW
                     FELLNLPKKIIFVGHDWGACLAFHYSYEHQDKIKAIVHAESVVDVIESWDEWPDIEEDI
                     ALIKSEEGEKMVLENNFFVETMLPSKIMRKLEPEEFAAYLEPFKEKGEVRRPTLSWPRE
                     IPLVKGGKPDVVQIVRNYNAYLRASDDLPKMFIESDPGFFSNAIVEGAKKFPNTEFVKV
                     KGLHFSQEDAPDEMGKYIKSFVERVLKNEQ"
     regulatory      3752..3761
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(3806..4163)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3820..3955
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     enhancer        3972..4163
                     /label=SV40 enhancer
                     /note="enhancer for the SV40 early promoter (Herr, 1993)"
     primer_bind     4120..4140
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     polyA_signal    4408..4529
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4445..4464)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4499..4518
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     RBS             9262..9270
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(10176..11828)
                     /codon_start=1
                     /product="firefly luciferase"
                     /label=luciferase
                     /note="synthetic luc2 version of the luciferase gene"
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
     regulatory      11825..11834
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(11875..12374)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(11923..11942)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     12316..12339
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    12431..12714
                     /label=cer region
                     /note="ColE1-derived recombination site that helps to 
                     maintain plasmids as monomers"
ORIGIN
        1 ttgaaatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccggttg
      721 ggcgtcgctt ggtcggtcat ttcgaattac caatgcttaa tcagtgaggc acctatctca
      781 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
      841 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
      901 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
      961 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     1021 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     1081 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     1141 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat agttgtcaga
     1201 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     1261 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     1321 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     1381 ccacatagca gaactttgaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     1441 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     1501 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     1561 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     1621 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     1681 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     1741 gtctaagaaa ccattacgta gaaagccagt ccgcagaaac ggtgctgacc ccggatgaat
     1801 gtcagctact gggctatctg gacaagggaa aacgcaagcg caaagaggat ccttatcgct
     1861 atcgattcac acaaaaaacc aacacacaga tgtaatgaaa ataaagatat tttattgcgg
     1921 ccatcgtgat ggcaggttgg gcgtcgcttg gtcggtcatt tcgaacccca gagtcccgct
     1981 cagaagaact cgtcaagaag gcgatagaag gcgatgcgct gcgaatcggg agcggcgata
     2041 ccgtaaagca cgaggaagcg gtcagcccat tcgccgccaa gctcttcagc aatatcacgg
     2101 gtagccaacg ctatgtcctg atagcggtcc gccacaccca gccggccaca gtcgatgaat
     2161 ccagaaaagc ggccattttc caccatgata ttcggcaagc aggcatcgcc atgggtcacg
     2221 acgagatcct cgccgtcggg catgcgcgcc ttgagcctgg cgaacagttc ggctggcgcg
     2281 agcccctgat gctcttcgtc cagatcatcc tgatcgacaa gaccggcttc catccgagta
     2341 cgtgctcgct cgatgcgatg tttcgcttgg tggtcgaatg ggcaggtagc cggatcaagc
     2401 gtatgcagcc gccgcattgc atcagccatg atggatactt tctcggcagg agcaaggtga
     2461 gatgacagga gatcctgccc cggcacttcg cccaatagca gccagtccct tcccgcttca
     2521 gtgacaacgt cgagcacagc tgcgcaagga acgcccgtcg tggccagcca cgatagccgc
     2581 gctgcctcgt cctgcagttc attcagggca ccggacaggt cggtcttgac aaaaagaacc
     2641 gggcgcccct gcgctgacag ccggaacacg gcggcatcag agcagccgat tgtctgttgt
     2701 gcccagtcat agccgaatag cctctccacc caagcggccg gagaacctgc gtgcaatcca
     2761 tcttgttcaa tccctccgga gcctccgcca cctgatccgc cacctccgct cccaccaccg
     2821 gtctgctcgt tcttcagcac gcgctccacg aagctcttga tgtacttacc catttcatct
     2881 ggagcgtcct cctggctgaa gtggaggccc ttcaccttca cgaactcggt gttagggaac
     2941 ttcttagctc cctcgacaat agcgttggaa aagaacccag ggtcggactc gatgaacatc
     3001 ttaggcagat cgtcgctggc ccgaaggtag gcgttgtagt tgcggacaat ctggacgacg
     3061 tcgggcttgc ctcccttaac gagagggatc tcgcgaggcc aggagagggt aggccgtcta
     3121 acctcgccct tctccttgaa tggctccagg taggcagcga actcctcagg ctccagtttc
     3181 cgcatgatct tgcttgggag catggtctcg acgaagaagt tattctcaag caccattttc
     3241 tcgccctctt cgctcttgat cagggcgata tcctcctcga tgtcaggcca ctcgtcccag
     3301 gactcgatca cgtccacgac actctcagca tggacgatgg ccttgatctt gtcttggtgc
     3361 tcgtaggagt agtgaaaggc cagacaagcc ccccagtcgt ggcccacaaa gatgattttc
     3421 tttggaaggt tcagcagctc gaaccaagcg gtgaggtact tgtagtgatc caggaggcga
     3481 tatgagccat tcccgctctt gccggactta cccattccga tcagatcagg gatgatgcat
     3541 ctagccacgg gctcgatgtg aggcacgacg tgcctccaca ggtagctgga ggcagcgtta
     3601 ccatgcagaa aaatcacggc gttctcggcg tgcttctcgg aatcatagta gttgatgaag
     3661 gagtccagca cgttcatttg cttgcagcga gcccaccact gaggcccagt gatcatgcgt
     3721 ttgcgttgct cggggtcgta caccttggaa gccatggtgg ctccagcctt tggttcgaga
     3781 ctgttgtgtc agaagaatca agctttttgc aaaagcctag gcctccaaaa aagcctcctc
     3841 actacttctg gaatagctca gaggccgagg cggcctcggc ctctgcataa ataaaaaaaa
     3901 ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg
     3961 gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc
     4021 ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt
     4081 gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc
     4141 acagctggtt ctttccgcct cagaaggtac ctaaccaagt tcctctttca gaggttattt
     4201 caggccatgg tgctgcgcag atccgcgtat gcggtgtgaa ataccgcaca gatgcgtaag
     4261 gagaaaatac cgcatcagga aattgtaagc gttaatattt tgttaaaatt cgattttacc
     4321 acatttgtag aggttttact tgcttaaaaa aacctcccac atctccccct gaacctgaaa
     4381 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     4441 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     4501 ggtttgtcca aactcatcaa tgtatcttat catgtctgct cgaagcggcc gccccaaggg
     4561 gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt cgggctttgt
     4621 tagcagccgg atcagcttgc atgcctgcag gtcgactagt gacctcagtg agatatttgg
     4681 taccatggtg taagctgata gcacaagttc tggcgacagg tagcacgtat ctgagagaca
     4741 cctgcaagca cacactgtcc cagctggtac ccataatgct gctgttcctg ctgctgctcc
     4801 tgctttcctc tcttctcgct ttaaatgatt gttagcaagt tagattttcc tggagcttcg
     4861 gaagaaatga aaatggaaac atgatgaaga tatagtcact gaatcaatga gaaaaataat
     4921 gaacatatga ataccaccta aaaatacact gaataaagta caccatatag cattattttt
     4981 ataggaaata tttcatgtcc cttaaatatt ctttgttagt tatagggatg gacagtttat
     5041 gttaagcact tagcttaaac aatccgttta tattagcact gtatcccttg tgccatccaa
     5101 cattttgtat gtttttgtaa acagttcata tacagtacat ttctgtactg ctttctttaa
     5161 tgtatacatg ccttgtttaa cttggaatct attattatta atcaattgac tattaaatct
     5221 ggttaaatag ttcacctgga ttaacagtat tgttggacag tcctaaaaat ggccagattg
     5281 tggaacagct gttgaatgta atacttccaa aatgtacata tctttcccca cgtctgtttc
     5341 actggttcgt tcatttgttt gtttcttaaa gtcaggtgct ctgtcagact aacctagaga
     5401 gctgttatgg tagagaaagt tatcatatgt gtgtggcatg aaatcaagaa tacacctatg
     5461 aagttagtcc atatactttg caactcctta gagtactttt ttccttaatt aaggaagtag
     5521 tccttgcact tctaatctta catagcatcc atacttagaa tttggcatat catctgggat
     5581 tttgccaata tacgtcaaag ccctttaaga gtcatggtaa ggagatgggt gaaggaaaat
     5641 ttagcaacag gtaattgaag tcctattgga tatttcatgt ttaaatagat attctatatt
     5701 aaacactaat ttaaatgtaa taaaggccaa aggcctctgt atgaaattgg atttaaactt
     5761 tcttatttta gggaataaaa cattattgat caaacagtat ctgttctaac ctaaaattat
     5821 aggtagggca ggctaagtga acagcattga gtattttctg aatccctcat gataatttat
     5881 agccacatac tgcttccttt gacttcagga atgatcagtt ttcataatgg ccactgggcc
     5941 tgcttagatt gcagtattca ttatctgcat ctaatttggt agtttccaca atcgtatttg
     6001 atgaaagaaa cttcagtccc cattattacc tgtgtctttg ccaagctgcc tagcatacat
     6061 cagtatgtaa tgtaaaagac atatgagcaa gaaaaaagtg atttaactta cctcatcaag
     6121 aatgtgcccc tacaggccgg gcgcagtggc tcacgcctgt aatcccagca ttttgggagg
     6181 ccgaggcagg tggatcacct gaggtcagga gtttgagacc agcctggcca acatggtgaa
     6241 accccgtctc tactaaaaaa tacaaaaatt agctgggcat ggtggcgtgc acctgtaatc
     6301 ctagctactc aggaggctga ggcaggagaa tcgcttgaac ctgggaggtg gtggttgcag
     6361 tgagccaaga tcacaccatt gcactccagc ctgggtgaca gagtgagact ctgtctcaag
     6421 aaggaaaaaa aaaagaatgt actcatacag atttttgtta gtcatcatgt aataagccat
     6481 ttatatcaga aacctttttt cctgccacag aagctttaaa ttttccccta agagtacaaa
     6541 gccaagagag tgtggtttat ctaaatgtcc tgaaatccac ttgtcattcc ttaatttctc
     6601 ctctatgcaa ttaaagagaa aggggaaatc tataacattt cttttgccca agtgacacaa
     6661 aaatgattac tgcatagaaa tcaaatgtaa gtttagcttc taaatgaaat aatatatagg
     6721 agggatatca gaatgtctag atagagaaag tattttcaaa ttcttgaata tatggatgct
     6781 attaaaagct gctttccatt gcctggaaag agagcttttt tccctcctct attcagtgtt
     6841 tcaaactttg gtcatctcaa attattgtca cttttaaaat gttaatgtca ttctgagcag
     6901 aaatagatat attttcagat ttttttacac tgatgctact ccgtaattgc ttaggagtat
     6961 tgtccatcat agtattttag aactagaagt aaacacataa atccttataa attacccaat
     7021 ccaactttct catattgcat gtagagaacc tgagacacat atcaaagtct agaatcctta
     7081 acttctcccc attttgtttt tttccgcctc atcacaccat gctgcctttc cctcatatga
     7141 tcaccaacca gtagctgagt tcaaaaacta catatttctt gggcagtcct ctactggtat
     7201 gtgatgccaa cttttatttc tttcgaagat aattgccttc tttgcctagc caagcagcca
     7261 attttattgg agaaaagcaa gttatatgtt cacaaaatgg aaggctttct acctaaattt
     7321 atttcatctg tggtcctctt attctcttcc attgcttttg acatacagtg gaaaaacttg
     7381 gccaacgatg tgctctaaat atagtgcttg tgttgggtta acttttccat taatttctca
     7441 acatcaatta ttcaggcccc tacagttgtg gtattgaggc ccctaaaggc ttctgccttt
     7501 caccaattag tgctgccagt tactgaggct tctcccaaaa ggtatcagtc cagtcttaaa
     7561 gatatttcat tgtgaaagaa gaaactaaac tatcaggcct cttttacaaa atgagagatt
     7621 gaatttaagc ttgtcaagca cgtactggaa ggtatgaatt acactaccat gtgttttgta
     7681 tcttcccttt caagtgatga tgttaaatga aggtaagttt ttcatccttt tttaattttt
     7741 gttttttata aatcatttca gctttttctg gtttatagag gtgtcttatt tctaatgcaa
     7801 cagaccccca actttaacag atttgatatg gatgcattta ttcacaagca accccaaaag
     7861 tccaaaaatg taataatttt gacaaggccc aaagttgaga tgctatgaag cttgtgtgtg
     7921 tgaaaagtca gttatgattg tctggagaga agctgtggtg tgtatgctgt agagtttcca
     7981 catttcacat gcagtgtact ccaaaaagcg ggtttgggtc aaccatttcc catctctttt
     8041 taagaagtga ctgctgttgg ggccagggga catcatggga ggtggggctg tccagttagc
     8101 tgtgccctgc accttcagcc caggaaagat ttgaacagag caaaggcttc aagagagggc
     8161 agaatgtatt cggcagaaaa gggactcagg taagcccagc ctgggatgag agcagaaaag
     8221 cattcaagat ttgcaggcct tgctatggat gcgcttaatc accatggagg ccagcaatac
     8281 ccatctcagc atggctttga tattctctac ttcctggcct ttaaaaatga cctataattt
     8341 ttcagtttgc tttactatat tttataaaga aaattctatc ttatggttga ttgagcattg
     8401 agacttatga aggcattagg atagatagct caggaatgta aaggttcaga aaaggtctgt
     8461 tttctcagat taacaaatat gatggattcc atggctgacc ttggtgctta aaccaggagg
     8521 tttcaatcta gtcctagagt tgtgtccctc tgaaaggccc aatgccatgt aactaacttt
     8581 aaactggata tatactttga gccttactta attcacagat aagttgactt aactcagtat
     8641 ttttatttca attaatgaaa acagtcctct tttcaacccc aggttgctta cattttgctg
     8701 gtctccccaa gtgaccattg gtggagacca attaatgaag gaatgaaatt cactttattg
     8761 ggactgtggt attcaacaga gccacactta accacttttt ccaatgaaga atctccagaa
     8821 tgataatgcc caaatatgga tggccaagaa gaatttgtat ctacggtgtg ctttatgtgt
     8881 ttttgacact gctgtattct gtgtgatcaa gtgatttgca gctggttcca atgtgactga
     8941 gtgttctcaa agatttctag taactaagtc aacttaattt tcttaagcct ggtattacta
     9001 tcagcctcac atttaccact ttgattctag ttttttaact gttcataaca gggcataccg
     9061 agggttggga tgagagccta cttcctacct cttaaggcac tttcctcatt attttgccat
     9121 ataatcttga actgcatgat aagctgttta aatgtccatg acttctccca gagcaactag
     9181 caaagtatat gacattttga atagagatta gtggaaagga aaatgtagag atttaagttc
     9241 agaggtacaa acctcaataa aacctcctta gagcacatac catgagtaga gtatatgtaa
     9301 acgtatatga gaagagagtc gctataatag tcctctctaa gtagattagt tcattgtcta
     9361 atggaagtga acatctttac tcctgttatc accatagtac tgaatgaact gtataatgag
     9421 tttttcaaac aagtatttaa aatagaactt tcacgtacaa agcaagggca taaagatttt
     9481 ggtttgtgtt tctgtaagtg tagtttacca tctaaagctt tggtttttaa ttttaaaaaa
     9541 agcttcaagg tattgccatt ccttagcatc cttattgatt atgggtgatg agtttttaac
     9601 tcttaaaatc atatacgtgt attagtttat cttaactgtt gccctaagca aaagggaagg
     9661 tatacaccta agggatgtac ttattttgca tttttggtca tgggtgggaa tggggtgggt
     9721 gcctacttaa atagttttta aaaaagaaaa aaatcacaaa tatttttcta ctgttatata
     9781 tgatcttcct ttatactagt ttctctctag taaggcatgc cagaagccca agataccata
     9841 tcatgaattc ttacatattg taccttttgt tggtggttaa atcgcatcag atgcttggca
     9901 ttgctgccat aattataaaa tgcttgtaaa ggatcaaata tcactaaata ctttaaattg
     9961 ttttacttaa gagtctaatc tgggaagttt tcaaatcata ctattaatgt gtaatctaag
    10021 ctcttcagat gtatccatga ataatcctgg aacaatattg cttgtattcc tgtcatagaa
    10081 caggttttgt aatctttaaa agaaatgaaa atttatataa taaagtttca aatcaatgca
    10141 actcgaggct agcgagctcg tttaaacaac tagaattaca cggcgatctt gccgcccttc
    10201 ttggccttaa tgagaatctc gcggatcttg cgggcgtcca acttgccggt cagtccttta
    10261 ggcacctcgt ccacgaacac aacaccaccg cgcagcttct tggcggttgt aacctggctg
    10321 gccacatagt ccacgatctc cttctcggtc atggttttac cgtgttccag cacgacgact
    10381 gcggcgggca gctcgccggc atcgtcgtcg ggcaggccgg cgaccccggc gtcgaagatg
    10441 ttggggtgtt gcagcaggat gctctccagt tcggctgggg ctacctggta gcccttgtat
    10501 ttgatcaggc tcttcagccg gtccacgatg aagaagtgct cgtcctcgtc ccagtaggcg
    10561 atgtcgccgc tgtgcagcca gccgtccttg tcgatgagag cgtttgtagc ctcggggttg
    10621 ttaacgtagc cgctcatgat catggggcca cggacgcaca gctcgccgcg ctggttcaca
    10681 cccagtgtct taccggtgtc caagtccacc accttagcct cgaagaaggg caccaccttg
    10741 cctactgcgc caggcttgtc gtccccttcg ggggtgatca gaatggcgct ggttgtttct
    10801 gtcaggccgt agccctggcg gatgcctggt aggtggaagc gtttggccac ggcctcacct
    10861 acctccttgc tgagcggcgc cccgccgctg gcgatctcgt gcaagttgct taggtcgtac
    10921 ttgtcgatga gagtgctctt agcgaagaag ctaaatagtg tgggcaccag cagggcagat
    10981 tgaatcttat agtcttgcaa gctgcgcaag aatagctcct cctcgaagcg gtacatgagc
    11041 acgacccgaa agccgcagat caagtagccc agcgtggtga acatgccgaa gccgtggtga
    11101 aatggcacca cgctgaggat agcggtgtcg gggatgatct ggttgccgaa gatggggtcg
    11161 cgggcatgac tgaatcggac acaagcggtg cggtgcggta gggctacgcc cttgggcaat
    11221 ccggtactgc cactactgtt catgatcagg gcgatggttt tgtcccggtc gaagctctcg
    11281 ggcacgaagt cgtactcgtt gaagccgggt ggcaaatggg aagtcacgaa ggtgtacatg
    11341 ctttggaagc cctggtagtc ggtcttgcta tccatgatga tgatcttttg tatgatcggt
    11401 agcttctttt gcacgttgag gatcttttgc agccctttct tgctcacgaa tacgacggtg
    11461 ggctggctga tgcccatgct gttcagcagc tcgcgctcgt tgtagatgtc gttagctggg
    11521 gccacagcca caccgatgaa cagggcaccc aacacgggca tgaagaactg caagctattc
    11581 tcgctgcaca ccacgatccg atggtttgta ttcagcccat agcgcttcat agcttctgcc
    11641 agccgaacgc tcatctcgaa gtactcggcg taggtaatgt ccacctcgat atgtgcgtcg
    11701 gtaaaggcga tggtgccggg caccagggcg tagcgcttca tggctttgtg cagctgctcg
    11761 ccggcggtcc cgtcttcgag tgggtagaat ggcgctgggc ccttcttaat gtttttggca
    11821 tcttccatgg tggctttacc aacagtaccg gattgccaag cttgggctgc aggtcgaaag
    11881 gcccggagat gaggaagagg agaacagcgc ggcagacgtg cgcttttgaa gcgtgcagaa
    11941 tgccgggcct ccggaggacc ttcgggcgcc cgccccgccc ctgagcccgc ccctgagccc
    12001 gcccccggac ccaccccttc ccagcctctg agcccagaaa gcgaaggagc aaagctgcta
    12061 ttggccgctg ccccaaaggc ctacccgctt ccattgctca gcggtgctgt ccatctgcac
    12121 gagactagtg agacgtgcta cttccatttg tcacgtcctg cacgacgcga gctgcggggc
    12181 gggggggaac ttcctgacta ggggaggagt agaaggtggc gcgaaggggc caccaaagaa
    12241 cggagccggt tggcgcctac cggtggatgt ggaatgtgtg cgaggccaga ggccacttgt
    12301 gtagcgccaa gtgcccagcg gggctgctaa agcgcatgct ccagactgcc ttgggaaaag
    12361 cgcctcccct acccggtaga tctcgatcct ctacgccgga cgcatcgtgg ccggagtact
    12421 gcggtttatc tcgatggcta cgagggcaga cagtaagtgg atttaccata atcccttaat
    12481 tgtacgcacc gctaaaacgc gttcagcgcg atcacggcag cagacaggta aaaatggcaa
    12541 caaaccaccc gaaaaactgc cgcaatcgcg cctgataaat tttaaccgta tgaataccta
    12601 tgcaaccaga gggtacaggc cacattaccc ccacttaatc cactgaagct gccatttttc
    12661 atggtttcac catcccagcg aagggccatc cagcgtgcgt tcctgtattt ccggactata
    12721 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    12781 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttc
//
