LOCUS       sequence-469283-        9942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of CAMSAP3 (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9942)
  AUTHORS   Flinois A, Mutero-Maeda A, Montessuit S, Citi S
  TITLE     Evidence for an interaction of paracingulin with microtubules.
  JOURNAL   MicroPubl Biol. 2024 Oct 14;2024:10.17912/micropub.biology.001341. 
            doi: 10.17912/micropub.biology.001341. eCollection 2024.
  PUBMED    39469042
REFERENCE   2  (bases 1 to 9942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "MicroPubl 
            Biol."; date: "2024-10-14"; volume: 
            "2024:10.17912/micropub.biology.001341. doi"; pages: " 
            10.17912/micropub.biology.001341. eCollection 2024"
COMMENT     Sequence Label: GFP-mCAMSAP3-Myc
FEATURES             Location/Qualifiers
     source          1..9942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..3304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3320..3349)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(7121..7831,7832..7834,7835..7837))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7121..7831,7832..7834,7835..7837))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7163..7184)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7512..7531
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7771..7792
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7834..7843
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7845..7861)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7845..7861)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7894..7913)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7895..7913)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7958..8161)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7987..8007)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8162..8541
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8713..8732
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8807..8911
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8912..8980,8981..9772)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9130..9149)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccgcc acccttcttg ggcgtggtgg gcttcttgct ttgccaaagg tgtccctgga
     3421 tagtgaaggc gtctacactc atagacatgg ttttagcagg gatctgggtg aaccgtttgc
     3481 gatccgagtt gtacttatag atgccttcga ccatggcagg agtgacggtc cgaggcccat
     3541 agcctgccag cctcgatagc tcctctgtct ccccagacag agtgtagagg gcccggaact
     3601 ggcagcttga gtcccgaaag agaatcagga agtggttggc cttgcttttc tcgatttcct
     3661 ctagaattct gttcttctgg ggctcattca ccttgcctgc caggcagcag tgtgacaagg
     3721 cattgtggat gataaacttg ttagacttgg cgctgggttc cttgtatagc cggggacctg
     3781 tgtactcagg cactgatgct ggggaggatg cattgcttcc attctcccag tcacgttctc
     3841 gactgccagg caggcggctt ggagacatga ggcctgatgg agaaggggcc cgagaggtgg
     3901 gcctcttcac accaaggtta ttgggagcct cgttggccac cgtagatagg gacagcgtgg
     3961 actgggaata gaccttgctg agccgtgaac ccagcaggcc gcgggctggg cttcgtgcca
     4021 aggccgagtc atcacagcaa ccagagcgtg gccgggtggc cctgcgcccg ccccgccctg
     4081 gtcccccggt ccctgaggcc cggggccgta gcaccttatc aaggtcatcc atcagtttca
     4141 gttgtgctcg gcgttcatac tcaagtcttg tgaagtcccc ccgccggggg cccacctcct
     4201 cctcagctgg ggccatggct ctggtagtag gagccaaggt ggccactgga gccgcagagg
     4261 ctacaacagg agttgtaaag gccaagcctg gagcctcctg agccagcctg gcggcctcct
     4321 cccgtttctg ctccttctct gcctcctgcc actgtttgcg ccgccgggct tcctctaccc
     4381 gcctctgctg acgctccagc aggctagctc gcttctgagc catctcatcc tcaggcttgt
     4441 cttcgtcctt ataaaagaat ccaagccctg atcggggctc tccctcagaa gatgcctctt
     4501 cctctaagga atcctcagcc ccaggggggc tcccatctcc ttcctcatca gcagcaggct
     4561 cccccaggct ggctagaggg atctcaatga gggcaggctt ggtggtgggc tcctcgggag
     4621 gtgtcccctc cgacaggagg atagaggagc gagtctgtac aggcacctgg ctgggtgaaa
     4681 acttgcgtag gtgagggagg ctgtctacat catggggtgg tgtgagtacc cttgtcaaag
     4741 gtgcaagctt cagctctgcc ggccttgcat gttttggact acggggtgtt gggctggggc
     4801 ctggcccagg gctgcgtcgg gcagctgggg cacgacgggc agggctgggg gatgctgctt
     4861 taggcccagt ggcgggtcca ggaatgaccc aggctgcagg tggtggggca ggtccaggag
     4921 cttctggagg ggctagaagc cgctgttgct ggtctgtgag cctctgcatg tcccgctgca
     4981 gagagctcag agcggcactc agcttactga ctgctctatt gtaatcccca agtccctctg
     5041 ggggcactgg gcccaggtct ggagagaagg taactgactt gtgccgtaag gacggctcac
     5101 cctggccctc acctgctggc cgttccccac caggaactga gcccagctca gcttcctcct
     5161 cggcctcccc tgcagcctcc cgaggctgca cctgcaggaa agcgctcttg cccagcctct
     5221 gcctatgctt ggcaaagatt gcctcaatgc gtcgtttctg tgcctctatg gctcggcgct
     5281 tctcctccag cctggctccc agctcactca tctctgagct caaggcctca ggtgctacgg
     5341 gggtggatgt tggagacccc aatcctgact cagcctcagc cttcaccagc tgtttcttgc
     5401 gttcagcaaa actggtcatc ttcacttcgg agttgttggc tgctggggat gaagccacag
     5461 ctttggggga accctcggat gggggtggtg gtttcagtat ctctcctgtt gagcagggag
     5521 atgattttga agggttctca gggtgtgata tatagatagg cgccttgttg agcctatcag
     5581 acagcggctt tgaggctcct tcgggagggt agggggagag tggtggtttg gagagaccct
     5641 caggagaatg gaggtagaaa ctgccatcag cagccccatc agggagcagt cggggctcag
     5701 cactgtgaat gatctgcagg gcctcttcaa tcgtgggtag gtctccagat tctggggctg
     5761 gctgagcaga attccgggat gatgttgaca caggacgtgg aggacccaga ctgtctgagc
     5821 tgacggagcg gagcaggaca ggatctccca tgacgacatc cacatcgctg tccaggccaa
     5881 agggagtgct gaacgacaca gcctgggaga ggggacggct aagctgacag ttccaggctt
     5941 tgccaagagc ctccatgtga gacattgatg gagaggactt cagggagcct gtggatcctc
     6001 ggagtggaga ctggggacca ccaggtgaca gaagtgggtg acggaagttg aagacaggag
     6061 aactgctgcc actgttgttc tgagatggaa cagtctctgt attccggggg gagacagcat
     6121 gtccatcagg caagtctttg gcctgcacaa aatcaggctt cagaacctca aagcacatat
     6181 acatctcagc caacagcacc accaggttga ccttgagggg tggtgggaca tacagcaagt
     6241 cttcaaggga cagggggcag cctcgaggaa gatgggaggc acagaagtct tgcaccagct
     6301 ggaggttgta tagactgtct gccacagaca tggggtcctt gaggcacacc tcctcaagtc
     6361 gtaatagctg gggacaatag cagtggatgg tggcagccag tgctgcccca ctggccaggt
     6421 cctgaagggt agtcacattt ggaaagcagg gagcccttcg ggcaatggca cggtccttgc
     6481 gatatcgaat cgagggctgg gcaggagagg ccccatccag aggggctgca ggagatgcac
     6541 gctgggctgc ttcttgttct gtcttctcct gcagccgccg aacagttgtg tctacccaga
     6601 aaaggagttt gtgctccaag ctgctcagag ccaagggacc aggtagtgtc tttgtccact
     6661 caaatgagaa ggcaaccatg agagcatcga tgacagctag gtgggctccc atgagaattg
     6721 gctggtgttt caggtcagcc tcgcgcactg ggtgctcagg cagtgaaggc accgtgcccc
     6781 tcctcgccag caaggccaac agagcagagg gactggggga tggctcgagc tgtggcggtg
     6841 cctggcgcca ggcccgacag tatagttccg cagagagcag cagccgcgtc actgggggct
     6901 tcacgtgctc ctgtgcgtac tgatcggtgt agaagggttc ccacagctcc ggaggcacat
     6961 gctctgctcc cccgaatgcg gcccgcagca cccacgccag actcgccgcc gccttggctc
     7021 gagagaaatc gtactggtcc aaagacttga tctcggggac caggaaagtc ctccgcagtg
     7081 gcccggaccc tgcgggcgcc gcttccacca tgcggccgct cttgtacagc tcgtccatgc
     7141 cgagagtgat cccggcggcg gtcacgaact ccagcaggac catgtgatcg cgcttctcgt
     7201 tggggtcttt gctcagggcg gactgggtgc tcaggtagtg gttgtcgggc agcagcacgg
     7261 ggccgtcgcc gatgggggtg ttctgctggt agtggtcggc gagctgcacg ctgccgtcct
     7321 cgatgttgtg gcggatcttg aagttcacct tgatgccgtt cttctgcttg tcggccatga
     7381 tatagacgtt gtggctgttg tagttgtact ccagcttgtg ccccaggatg ttgccgtcct
     7441 ccttgaagtc gatgcccttc agctcgatgc ggttcaccag ggtgtcgccc tcgaacttca
     7501 cctcggcgcg ggtcttgtag ttgccgtcgt ccttgaagaa gatggtgcgc tcctggacgt
     7561 agccttcggg catggcggac ttgaagaagt cgtgctgctt catgtggtcg gggtagcggc
     7621 tgaagcactg cacgccgtag gtcagggtgg tcacgagggt gggccagggc acgggcagct
     7681 tgccggtggt gcagatgaac ttcagggtca gcttgccgta ggtggcatcg ccctcgccct
     7741 cgccggacac gctgaacttg tggccgttta cgtcgccgtc cagctcgacc aggatgggca
     7801 ccaccccggt gaacagctcc tcgcccttgc tcaccatggt ggcggatcca ctagttctag
     7861 agggcccgtt taaacgctag ccagcttggg tctccctata gtgagtcgta ttaatttcga
     7921 taagccagta agcagtgggt tctctagtta gccagagagc tctgcttata tagacctccc
     7981 accgtacacg cctaccgccc atttgcgtca atggggcgga gttgttacga cattttggaa
     8041 agtcccgttg attttggtgc caaaacaaac tcccattgac gtcaatgggg tggagacttg
     8101 gaaatccccg tgagtcaaac cgctatccac gcccattgat gtactgccaa aaccgcatca
     8161 ccatggtaat agcgatgact aatacgtaga tgtactgcca agtaggaaag tcccataagg
     8221 tcatgtactg ggcataatgc caggcgggcc atttaccgtc attgacgtca atagggggcg
     8281 tacttggcat atgatacact tgatgtactg ccaagtgggc agtttaccgt aaatactcca
     8341 cccattgacg tcaatggaaa gtccctattg gcgttactat gggaacatac gtcattattg
     8401 acgtcaatgg gcgggggtcg ttgggcggtc agccaggcgg gccatttacc gtaagttatg
     8461 taacgcggaa ctccatatat gggctatgaa ctaatgaccc cgtaattgat tactattaat
     8521 aactagtcaa taatcaatgt caacgcgtat atctggcccg tacatcgcga agcagcgcaa
     8581 aacgcctaac cctaagcaga ttcttcatgc aattgtcggt caagccttgc cttgttgtag
     8641 cttaaatttt gctcgcgcac tactcagcga cctccaacac acaagcaggg agcagatact
     8701 ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatagg ggatcgggag
     8761 atctcccgat ccgtcgacgt caggtggcac ttttcgggga aatgtgcgcg gaacccctat
     8821 ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata
     8881 aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct
     8941 tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa
     9001 agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa
     9061 cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt
     9121 taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg
     9181 tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca
     9241 tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa
     9301 cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt
     9361 gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc
     9421 cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa
     9481 actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga
     9541 ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc
     9601 tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga
     9661 tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga
     9721 acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga
     9781 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat
     9841 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt
     9901 ccactgagcg tcagaccccg tagaaaagat caaaggatct tc
//
