LOCUS       sequence-469284-        9831 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of CGN (dog) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9831)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 9831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: GFP-cCGN-Myc
FEATURES             Location/Qualifiers
     source          1..9831
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..3304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3320..3349)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3395..3424)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(7010..7720,7721..7723,7724..7726))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7010..7720,7721..7723,7724..7726))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7052..7073)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7401..7420
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7660..7681
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7723..7732
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7734..7750)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7734..7750)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7783..7802)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7784..7802)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7847..8050)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7876..7896)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8051..8430
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8602..8621
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8696..8800
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8801..8869,8870..9661)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9019..9038)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccggg ccccccctcg aggtcgacct aattcagatc ctcttctgag atgagttttt
     3421 gttcatcgat acaggagctg gtctgcaggt tgctctccgt aagcagcgat gcaatggaag
     3481 aagggtcgta gacactgtca aactcctcat ccgagctcag cccttcacgc tgggctgact
     3541 cagcagctga acgggaggct ttgcgccagg agtccttctc tagggttttg atccgggcct
     3601 ggagctgttc attgacctca tgttgctctt ccagctcacg ctgggccttc ttcctcaggc
     3661 catccagtcg ctcaatctcc tcttctgctt catccacctg ccgcttcaaa gccttcaccc
     3721 tcaggcttag ctggtccttc tggtcattga catgctgccg ctcatcatca atttggatgg
     3781 acagctcttt aaccctccgc tccagttttc ggttggtgga ctgaaggacc gtcttctccc
     3841 tctcttcagc ctgcagccgc tcctgcaact cctgattctg ggattcaagc tgagagaggc
     3901 tggcactggg cttctggaag ccttctgagc tggccaatcg gctcttcaag tccttgttct
     3961 gtctctctag ggagattttg tcacactcca ggtcctgtcg agcagatctc tcctgcatga
     4021 gctctgacct cagctggtct acctggtccc ggccacgagt cacacgttct gtcagcagct
     4081 ccacggtact cctctcctca tctaactctg cttccagccg tgagactttt tcctcaagac
     4141 ccttcagctg ccgggccctg tcatcctggg agcgcttctt gttctctgcc tcttgctcca
     4201 ggccctgcag tcgctgggcc agcagctctt tatccagcct tgctgtgtcc cggtcagcct
     4261 gggatgcctg cagggcctgc cggagcctct gggtctcatc ctgaagccgg ctcagccccc
     4321 ctgagttctt ctcagcctca gtggcccatt ccttggcctg acgctgggca tccgccacct
     4381 cccgccgggc cttctccttg taatcctcca gctgggcctg gagctgctgc agagcctgct
     4441 tagaatcgtt cccaatctgc tccagctcct tgttgagctg gcacacagtc ttgtccagca
     4501 agcgcttctg ttcctccagc tcagccttgc tccgccggag cgcctcccgc tgcttcccct
     4561 cctcctctag ggcccggttc agtgctagct gctcctgccc taggcgggcc aacccccgct
     4621 gggcctcctc cagccgggcc tccagtgccc gcttggcagc tgccaggctc ccctcctctt
     4681 cctgagcggc attcagggcc tcttcgagcc gctgtttctc tgcctctagc cgctgcacct
     4741 tgtcccgaag ccttgcctct gccacttccc cgccctctgc caggcctcgg gcctccttta
     4801 gctgctgttc cagacccaga atacgccgtc ggaactcgtc attctcctcc tgggtctccc
     4861 gaagtgtcac ttccactgct gcccgtcgct gccccagcac cgccacctct gcctctgctg
     4921 ccacctgagc cttggaagcc tcttcacagt cctgtctcag ttgctggagg gttttttgta
     4981 gctggaggtt ctgctgttcc aacccctggc ctcgatcagc ctcaaccttt agctgcttct
     5041 ccaattcccg ctcccggtga cgcccagcca cctcctggtt ctgctgctct tccttcagtt
     5101 ccttaagttc cttctgtgtc tggcacagct ccttcttaag ctcctccatt tggcgagtat
     5161 ctgcagcacc agctcgggcc tgccctaatt ccctttgtaa gacctctatc ttctccccaa
     5221 gctcctcttc tatctcctcc ttctccatcc gcagctgcag gagttcctgc ttagtggctc
     5281 taagttcatc cttgttcttc tggaacatgc tctgccaatg ccctgtctcc tctgaggtct
     5341 cctccagctc cttctgcagc tcccgcacca gggttgacat tttctgcctc tccacctcta
     5401 atgcagcatg gtcctgggtt gcatcctgta tactccggcg aagctgctct gcatctcgct
     5461 ggcactgctg ccggacatgt tccacctcct ggtcacggga ggccacctcc tccttcaggg
     5521 cccccttcag ggctgtcagc tcccgctccc gcagtctcag gtgctcctcc atccgctgct
     5581 tcccctctaa gacctcttcc agaagctctc gggcctctaa caggtccttc atgagcgcct
     5641 ccttagcagg ctcaggctcc tgggcctgtt tcagcttgtc ctgcagctcc ttcacctggg
     5701 cctccagccc acatcgtacc ctttcactct ggtccacaag gagcttcatg ttctggagct
     5761 ctttggtgct ctgctgggcc tcgccctttc tcctttccag cagctcctgc agttggctgc
     5821 actcttctgc cttctcctgc agctgccgct ccagcccaag ccgagatgac tctagcttcg
     5881 gccgtttctt cacctcctca tccagtttct gctgtagctc ctccactttt tggctgagct
     5941 cactctgccc agtcaggccc ttagcagatc cagaagtcat caccagaggc tgcatctgtt
     6001 ccagcaccaa attgaccttc ctcctcacag aggtttcggt ttctgagctt ccctccctca
     6061 ggatgctata gatagtggcc ttcacatggt ccacactgcc cggtggggcc gtctcctgct
     6121 ggtctcggag aaggtctgga gttgatttga actgtagcat gccagggtcc caggctctcc
     6181 cccgtggctc ctcaaaactc tgaagaaccc agtcctgggt ctgacgggaa cagctaaatc
     6241 caccaagagg gccctgattc tgggatgact gtttggagtt gcccatgtgg ctgtcacgct
     6301 ttgtgctagg agtccaatgg gtagggaact gatgttctcg ttcatcaagg gtatcccgtg
     6361 ggagccggtt gtccaggctt tggctccgct tgcgttgttc agggggcagt gtccgcatgc
     6421 gccggccagt gcggccccgg gcctgcccac cctggcgact atcaaacttg ttgatgagtg
     6481 agtccactga agacagggga gcagtgtcaa tagtgctccc tggggaagct acttcagggg
     6541 ccagctgcag caggctagtg ctcctggtgc tcggactcac aggggcaggg cctttcagtg
     6601 atgcctggga ctgggatcgg agtagccttc cattccaatg ggccccaagc tcctcatcag
     6661 acatgctgcc ctgtgagcgg gcaggaaatt ccttggcatg agagtaggtg ctttcaggga
     6721 gttcagagtc agagcttaga gctcctggag gccctctgtt gttggtcccc ttgatctgga
     6781 ccccaaagga gtcactacct tgctccccac tgttgagcac cacaaagggc tgcccagcaa
     6841 tgccctgcac acgcacagcg actccatagg tattggctct agcatcctta gcaggacgtc
     6901 gtccaccacg acgcagggtg tccatctctg cattacccac tggctctgtg atgaagcgaa
     6961 tctggactcc atggtctaca gggccccggg gctcagccat gcggccgctc ttgtacagct
     7021 cgtccatgcc gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc
     7081 gcttctcgtt ggggtctttg ctcagggcgg actgggtgct caggtagtgg ttgtcgggca
     7141 gcagcacggg gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc
     7201 tgccgtcctc gatgttgtgg cggatcttga agttcacctt gatgccgttc ttctgcttgt
     7261 cggccatgat atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt
     7321 tgccgtcctc cttgaagtcg atgcccttca gctcgatgcg gttcaccagg gtgtcgccct
     7381 cgaacttcac ctcggcgcgg gtcttgtagt tgccgtcgtc cttgaagaag atggtgcgct
     7441 cctggacgta gccttcgggc atggcggact tgaagaagtc gtgctgcttc atgtggtcgg
     7501 ggtagcggct gaagcactgc acgccgtagg tcagggtggt cacgagggtg ggccagggca
     7561 cgggcagctt gccggtggtg cagatgaact tcagggtcag cttgccgtag gtggcatcgc
     7621 cctcgccctc gccggacacg ctgaacttgt ggccgtttac gtcgccgtcc agctcgacca
     7681 ggatgggcac caccccggtg aacagctcct cgcccttgct caccatggtg gcggatccac
     7741 tagttctaga gggcccgttt aaacgctagc cagcttgggt ctccctatag tgagtcgtat
     7801 taatttcgat aagccagtaa gcagtgggtt ctctagttag ccagagagct ctgcttatat
     7861 agacctccca ccgtacacgc ctaccgccca tttgcgtcaa tggggcggag ttgttacgac
     7921 attttggaaa gtcccgttga ttttggtgcc aaaacaaact cccattgacg tcaatggggt
     7981 ggagacttgg aaatccccgt gagtcaaacc gctatccacg cccattgatg tactgccaaa
     8041 accgcatcac catggtaata gcgatgacta atacgtagat gtactgccaa gtaggaaagt
     8101 cccataaggt catgtactgg gcataatgcc aggcgggcca tttaccgtca ttgacgtcaa
     8161 tagggggcgt acttggcata tgatacactt gatgtactgc caagtgggca gtttaccgta
     8221 aatactccac ccattgacgt caatggaaag tccctattgg cgttactatg ggaacatacg
     8281 tcattattga cgtcaatggg cgggggtcgt tgggcggtca gccaggcggg ccatttaccg
     8341 taagttatgt aacgcggaac tccatatatg ggctatgaac taatgacccc gtaattgatt
     8401 actattaata actagtcaat aatcaatgtc aacgcgtata tctggcccgt acatcgcgaa
     8461 gcagcgcaaa acgcctaacc ctaagcagat tcttcatgca attgtcggtc aagccttgcc
     8521 ttgttgtagc ttaaattttg ctcgcgcact actcagcgac ctccaacaca caagcaggga
     8581 gcagatactg gcttaactat gcggcatcag agcagattgt actgagagtg caccataggg
     8641 gatcgggaga tctcccgatc cgtcgacgtc aggtggcact tttcggggaa atgtgcgcgg
     8701 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
     8761 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
     8821 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
     8881 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
     8941 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
     9001 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
     9061 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
     9121 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
     9181 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
     9241 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
     9301 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
     9361 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
     9421 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
     9481 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
     9541 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
     9601 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     9661 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     9721 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     9781 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt c
//
