LOCUS       sequence-469292-       10918 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of NM2A (dog) - with HA and FLAG tags in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10918)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 10918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: HA-cNM2A-Flag
FEATURES             Location/Qualifiers
     source          1..10918
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..429
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(88..107)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     298..319
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(438..458)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        443..772
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      623..758
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     promoter        820..867
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             886..1260
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    1390..1511
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1427..1446)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1481..1500
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(1560..1576)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1560..1576)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1573..1595)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1584..1600
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1608..1614,1615..1632,1633..1638))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1653..1674
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(1791..1808)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(1962..2550)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2042..2061)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2721..3512,3513..3581))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3344..3363
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3582..3686)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(3761..3780)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        3952..4331
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4332..4535
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     4486..4506
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     4580..4599
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        4580..4598
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             4661..4687
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             10574..10597
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(10643..10660)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    10649..10873
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
      481 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
      541 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
      601 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
      661 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga
      721 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc
      781 gggagcttgt atatccattt tcggatctga tcagcacgtg ttgacaatta atcatcggca
      841 tagtatatcg gcatagtata atacgacaag gtgaggaact aaaccatggc caagttgacc
      901 agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt ctggaccgac
      961 cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt ccgggacgac
     1021 gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac cctggcctgg
     1081 gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt gtccacgaac
     1141 ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg ggggcgggag
     1201 ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga gcaggactga
     1261 cacgtgctac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     1321 gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     1381 gcccacccca acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1441 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1501 aatgtatctt atcatgtctg tataccgtcg acctctagct agagcttggc gtaatcatgg
     1561 tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa catacgagcc
     1621 ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac attaattgcg
     1681 ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca ttaatgaatc
     1741 ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc ctcgctcact
     1801 gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc aaaggcggta
     1861 atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc aaaaggccag
     1921 caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag gctccgcccc
     1981 cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc gacaggacta
     2041 taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg
     2101 ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc
     2161 tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac
     2221 gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac
     2281 ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat tagcagagcg
     2341 aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg ctacactaga
     2401 agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa aagagttggt
     2461 agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag
     2521 cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc tacggggtct
     2581 gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt atcaaaaagg
     2641 atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta aagtatatat
     2701 gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc
     2761 tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac tacgatacgg
     2821 gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg ctcaccggct
     2881 ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag tggtcctgca
     2941 actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt aagtagttcg
     3001 ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt gtcacgctcg
     3061 tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt tacatgatcc
     3121 cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag
     3181 ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct tactgtcatg
     3241 ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt ctgagaatag
     3301 tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac cgcgccacat
     3361 agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa actctcaagg
     3421 atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa ctgatcttca
     3481 gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca aaatgccgca
     3541 aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct ttttcaatat
     3601 tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga atgtatttag
     3661 aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgacgtcgac
     3721 ggatcgggag atctcccgat cccctatggt gcactctcag tacaatctgc tctgatgccg
     3781 catagttaag ccagtatctg ctccctgctt gtgtgttgga ggtcgctgag tagtgcgcga
     3841 gcaaaattta agctacaaca aggcaaggct tgaccgacaa ttgcatgaag aatctgctta
     3901 gggttaggcg ttttgcgctg cttcgcgatg tacgggccag atatacgcgt tgacattgat
     3961 tattgactag ttattaatag taatcaatta cggggtcatt agttcatagc ccatatatgg
     4021 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
     4081 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
     4141 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
     4201 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
     4261 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
     4321 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
     4381 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
     4441 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
     4501 ggcgtgtacg gtgggaggtc tatataagca gagctctctg gctaactaga gaacccactg
     4561 cttactggct tatcgaaatt aatacgactc actataggga gacccaagct ggctagcgtt
     4621 taaacttaag cttggtaccg agctcggatc cgccaccatg tacccatacg acgtcccaga
     4681 ctacgctatc gatatggcgc agcaagctgc cgataagtat ctctatgtgg ataaaaactt
     4741 catcaataac ccgctggccc aggccgactg ggctgctaag aagctggtat gggtgccttc
     4801 cgacaagagc ggctttgagc ccgccagcct caaggaggag gtgggcgagg aggccatcgt
     4861 ggagctggtg gagaacggga agaaggtgaa ggtgaacaag gatgacatcc agaagatgaa
     4921 cccacccaag ttctccaagg tggaggacat ggcggagctc acgtgcctca acgaagcctc
     4981 cgtgttgcac aacctcaagg aacgctacta ctcagggctc atctacacct attcaggcct
     5041 gttctgtgtg gtcatcaatc cttataagaa cctgcccatc tactccgaag agatcgtgga
     5101 aatgtacaag ggcaagaaga ggcatgagat gcccccccac atctatgcca tcacagacac
     5161 cgcctacagg agtatgatgc aagaccgaga ggaccagtcc attttgtgca cgggtgagtc
     5221 tggagctggc aagacagaaa acaccaagaa agtcattcag tatctggcgc acgtggcctc
     5281 ctcacacaag agcaagaagg accagggtga gttggagagg cagctgctgc aggccaaccc
     5341 catcctggag gcctttggga acgccaagac tgtgaagaat gacaactcct cacgatttgg
     5401 caaattcatt cgcatcaact ttgatgtcaa tggctatatc gttggagcca acattgagac
     5461 ttatcttctg gagaaatcac gtgctatccg ccaagccaag gaggagcgga ccttccacat
     5521 cttctattac ctcctgtctg gggctggaga gcacctgaag actgatctcc tgttggagcc
     5581 atacaacaaa taccgcttcc tgtccaatgg gcatgtcacc atccctgggc agcaggacaa
     5641 ggacatgttc caggagacca tggaggccat gaggattatg ggcatcccag aagaagaaca
     5701 aatgggcttg ttgcgagtca tctcaggggt tctccagctc ggcaacatcg tcttcaagaa
     5761 ggaacgtaac accgaccagg cgtccatgcc cgacaacaca gctgcccaga aagtgtccca
     5821 cctgctgggg atcaacgtga cggatttcac ccgaggcatc ctcacgccgc gcatcaaggt
     5881 gggacgggac tacgtccaga aggcgcagac aaaggaacag gctgactttg ccatcgaggc
     5941 cttggccaag gccacctatg agcgaatgtt ccgctggctg gtgctgcgca ttaacaaggc
     6001 tctggacaag accaagaggc agggtgcctc tttcatcggg atcctggaca tcgctggctt
     6061 cgagatcttt gatctgaact ccttcgagca gctctgcatc aactacacca acgagaagct
     6121 gcagcagctc ttcaaccaca ccatgttcat cctggagcag gaggagtacc agcgtgaggg
     6181 catcgagtgg aacttcatcg actttggcct tgacctgcag ccctgcatcg acctcatcga
     6241 gaagccagca gggccccctg gcatcctggc cttgctggat gaggaatgct ggttccccaa
     6301 agccactgac aagagcttcg tggagaaggt ggtgcaggag cagggcaccc accccaagtt
     6361 ccagaagccc aagcagctga aggacaaagc cgatttctgc atcatccact atgcaggcaa
     6421 ggtggactac aaagccgacg agtggctgat gaagaacatg gaccccctga acgacaacat
     6481 cgccacgctt ctgcaccagt catcagacaa gttcgtctcg gagctgtgga aggatgtgga
     6541 ccgcatcatc ggcctggacc aggtggctgg catgtctgag acggcgctgc ccggggcctt
     6601 taaaacgcgg aagggcatgt tccggaccgt gggccagctc tacaaggaac agctggccaa
     6661 gctgatggcc acgctgagga acaccaaccc caactttgtc cgctgcatca tccccaacca
     6721 cgagaagaag gctggcaaac tggatccgca tttggtgctg gaccagctgc gctgcaacgg
     6781 ggtcctcgag ggcatccgga tctgccgcca gggcttcccc aacagggtgg tcttccagga
     6841 gttccggcag agatatgaga tcttgacacc aaattccatt cctaagggct tcatggatgg
     6901 gaagcaggcg tgtgtgctca tgatcaaagc cctggagctg gacagcaatc tgtaccgcat
     6961 cggccagagc aaggtcttct tccgggccgg cgtgctggcc cacctggagg aggagcggga
     7021 cctgaagatc acagacgtca ttatcgggtt ccaggcctgc tgcaggggct acctggccag
     7081 gaaggccttt gccaagcggc agcagcaact gacagccatg aaagtcctgc agaggaactg
     7141 cgccgcttac ctgaagctgc ggaactggca gtggtggagg ctcttcacca aggtcaagcc
     7201 actgctgcag gtgagccggc aggaggagga gatgatggcc aaggaggagg agctggtgaa
     7261 ggtccgcgag aagcagctgg ctgcagagaa caggctcacg gagatggaga cgctgcagtc
     7321 ccagctcatg gcagagaagc tgcagctgca ggagcagctc caggcagaaa cagagctgtg
     7381 tgctgaggct gaggagctac gggcccgtct gaccgccaag aagcaggagc tggaagagat
     7441 ttgccatgac ctggaggcca gggtagagga ggaggaggag cgctgccagc acctgcaggc
     7501 agagaagaag aaaatgcagc agaacatcca agaactggag gagcagctgg aggaggagga
     7561 gagcgcgcgc cagaagctac agctggagaa ggtgaccact gaggccaagc tgaagaagct
     7621 ggaggaagac cagatcatca tggaagacca gaactgcaaa ctggccaagg agaagaagct
     7681 tctggaagac agaatagccg agtttaccac caacctcatg gaagaggagg agaaatcaaa
     7741 gagcctcgcg aagctcaaga acaaacatga ggcgatgatc accgacctgg aagagcgcct
     7801 ccgaagagag gagaagcagc gtcaggagct ggaaaagacc cgtcggaagc tagaggggga
     7861 ctccaccgac ctcaatgacc agatcgccga gctgcaggct cagattgccg agctcaagat
     7921 gcagctggcc aagaaagagg aggagctgca ggccgccttg gccagagtgg aggaggaagc
     7981 cacccagaag aacatggccc tgaagaagat ccgagagctg gaatcgcaga tttccgagct
     8041 ccaggaagac ctggaatctg agcgagcttc taggaataaa gctgagaagc agaagcggga
     8101 ccttggggaa gagttggagg cgctgaaaac ggagctggag gacactctgg actcgacggc
     8161 tgcccagcag gagctcaggt ccaaacgcga acaggaggtg aacatcctga agaagaccct
     8221 cgaggaagag gccaggaccc acgaggccca gatccaggag atgaggcaga agcattctca
     8281 ggccgtggag gagctggccg agcagctgga gcagacgaaa cgggtgaaag ccaacttgga
     8341 gaaggcgaag cagaccctgg agaacgagcg aggggagctg gccaacgagg tgaaggtcct
     8401 gcagcagggc aaaggggact ctgagcacaa gcggaagaag gctgaggccc agctgcagga
     8461 actgcaggtc aagttcaccg agggggagcg agtgcgcaca gagctggcgg acaaggtcac
     8521 caagctgcag gttgagctgg acaatgtgat gggtctcctc acccagtctg acagcaagtc
     8581 cagcaaactc accaaggact tctctgctct ggagtcccag cttcaggaca cacaggagct
     8641 gctccaggag gagaaccgac aaaagctgag cctgagcacc aagctgaagc agatggagga
     8701 tgagaagaac tccttcaagg agcagctgga agaggaggag gaggctaagc ggaacctgga
     8761 gaagcagatc gccaccctcc atgcccaggt gacggacatg aagaaaaaga tggaggatgg
     8821 cgtggggtgc ctggagaccg cggaagaagc gaagaggaag ctccagaagg acctggaggg
     8881 gctgggccag cgctatgagg agaaggtggc tgcctacgac aagctggaga agacgaagac
     8941 gcggctgcag caggagctgg acgacctgct cgtggacctg gaccaccagc gccggactgc
     9001 ctccaacctg gagaagaagc agaagaagtt tgaccagcta ctggccgagg agaagaccat
     9061 ctctgccaag tatgcggagg agcgtgaccg ggccgaggca gaggcccggg agaaggagac
     9121 caaggcgctg tcgctggccc gggccctgga ggaggccatg gagcagaagg cggagctgga
     9181 gcggctcaat aagcagttcc gcactgagat ggaggacctc atgagctcca aggacgacgt
     9241 cggcaagagc gtccacgagc tggagaagtc caagcgggct ctggagcagc aggtggagga
     9301 gatgaagacc cagctagagg agctggagga tgaactgcag gccaccgagg acgccaagct
     9361 gcggctggag gtcaacctgc aggccatgaa ggcccagttc gagcgggacc tgcagggccg
     9421 cgacgagcag agtgaggaga agaagaagca gctggttaga caggtgcggg agatggaggc
     9481 tgagctggag gacgagaaga agcagcgctc gatggctgtg gctgctcgca agaagctgga
     9541 gatggatctg aaggacctgg aggcccacat cgactcagcc aacaagaacc gagacgaggc
     9601 catcaagcag ctgcggaagc tgcaggccca gatgaaggac tgcgtgcggg aactggatga
     9661 cacgcgtgcc tcccgtgagg agatcctggc tcaggccaag gagaacgaga agaagatgaa
     9721 gagcatggag gccgagatga tccagctgca ggaggaactg gcagctgccg agcgtgccaa
     9781 gcgccaggcc cagcaggagc gggacgagct ggcagacgag atcgccaaca gcagcggcaa
     9841 gggagctctg gcattggagg agaagcggcg cttggaggcc cgcattgccc agctggagga
     9901 ggagctggag gaggagcagg gcaacacgga gctggtcaac gatcggctga agaaggccaa
     9961 cctgcagatc gaccagatca acaccgacct gaacctcgag cgcagccacg cccagaagaa
    10021 cgagaacgcg cggcagcagc tggagcgcca gaacaaggag ctcaaggtca agctgcagga
    10081 gatggagggt accgtcaagt ccaagtacaa agcttccatc actgccctcg aggccaagat
    10141 cgcacagctg gaggagcagc tggacaacga gaccaaggag cgccaggcag cttgcaagca
    10201 ggtgcgtcgg gccgagaaga agctcaagga cgtgctgctg caggtggacg acgagcggag
    10261 gaacgccgag cagttcaagg accaggccga caaggcatcc acccgcctga agcagctcaa
    10321 gcggcagctg gaggaggccg aggaggaggc ccagcgggcc aacgcctccc gccggaagct
    10381 gcagcgcgag ctggaggacg ccaccgagac cgccgatgcc atgaaccgcg aggtcagctc
    10441 cctcaagaac aagctcaggc gaggggacct gccgtttgtt gtgccccgcc gggtggcccg
    10501 gaaaggtgct ggcgactgct cagacgagga ggtggacggc aaggcggacg gggctgaggc
    10561 caaagctgcc gaggattaca aggacgacga tgacaagtaa ggccgctcga gtctagaggg
    10621 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
    10681 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
    10741 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
    10801 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
    10861 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatcccc
//
