LOCUS       sequence-469297-       10146 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Paracingulin (dog) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10146)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 10146)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: GFP-cCGNL1-Myc
FEATURES             Location/Qualifiers
     source          1..10146
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..3304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3320..3349)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3395..3424)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             7001..7012
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             complement(join(7325..8035,8036..8038,8039..8041))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7325..8035,8036..8038,8039..8041))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7367..7388)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7716..7735
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7975..7996
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8038..8047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8049..8065)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8049..8065)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8098..8117)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8099..8117)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8162..8365)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8191..8211)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8366..8745
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8917..8936
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9011..9115
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9116..9184,9185..9976)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9334..9353)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccggg ccccccctcg aggtcgacct aattcagatc ctcttctgag atgagttttt
     3421 gttcatcgac gatctggctg gtggcagcgc tgtccttggg gaaggtgtag ctcagcggcg
     3481 cctcgtggag gctccccccg tccgtactga ggtcatcatc atccgtgtcg tccagcactt
     3541 tgctcggcag cttcttgagt cttaggtcct tcttcatgga attgagttgt ccctgcagct
     3601 gctcattcac atccatctgc tcctccagct ccctctgcag cttcttttta gaactttcta
     3661 gtctgtcgat ctcctcctcg gcctcctcta cttgtcgttt cattgctttc aagcgcaagc
     3721 taagctggtc cttctgatca gtcagagaca ggtgctcatc gtccacttgc atcactagct
     3781 ccttcacctt ccgctccagc cgtcggttgc tgagctggag gctggcccgg tccctctcct
     3841 cgttttccag acggtcctcc agctccgcga tcctggcctc catctgcacc accagcccct
     3901 ctttgctgga cctgtaggag ccttccagat ggataatccg gctctttaaa tctttgttct
     3961 gcctctccaa ggaaatcttg tcacactcca agtcctgctt gagagctctt tcctgcagga
     4021 gctcgctcct catctgctcc atctgttccc tgctccgagt gatcctctcc gagagcaaat
     4081 ccgagttatt tctttcttct tccagttcca tctccagctg agacaccttg tcctccatct
     4141 gcttgacgag tctgcttcgg tcatctttga ggtgactttt ggcctccagc tcgtattcca
     4201 ggtccttgag tgtctgctcc agaagctgcc tttttgtgag cgcctcatcc tgagctctct
     4261 ggtagtcccg taactcctcc tccatcagac gcatctcatc ctgcagtttc atggcagtca
     4321 gacgtgactt ttcagcctct agcgttttct ccttcaactg cctttgcatt tctgcaagtt
     4381 ccctgcgatt tttctccttg tactcatcta gctggttctg gagctccagt gttgatacac
     4441 gggacacctc aacaatgtca gccatctctt tctgcaattt ttcaatggtc ttgtccagat
     4501 gccatcgctc gttctccatc tcgttcttca gcctccttaa ctgctctttc tgctcagtct
     4561 catctttaag cttctcagac aactgcttct gctcctgggt ggcccgactc agatttctct
     4621 gcacgtcttc cagctcaccc tccagggtcc ttctgactga tgtggcttct ctttcttcct
     4681 ttcttgcatt cacaagggcc tcctctaatt gccggttttc tctctcgcat tttgtgagcc
     4741 tttcctttgc cttgacctca tcgtccttca tgtcagcaat ctgcctctgc agccgagcga
     4801 cgctctgctc cagctcttcg atgctttccc gcagcttctc attctcctcc tgcagagcct
     4861 tcacgtgcat ttcagccccc agctggctct gctctgaagt gttgctccga ctggccagga
     4921 cctcgacgtt cttggttgct tcttctacac tctccctcag ggcctgcagc tcggcgtcat
     4981 actgctcctt cagcttatcc atctccaggt cgtggctgga cacctcttcc ttcagggctc
     5041 ccttcagggc ggtcagttca cgctcccgct tcctcaagag gtcctcctgc tcctctttgg
     5101 cgatgagcag atcctgaaga tcctgttttg cctgtaagag ttcctcaatc agggctccct
     5161 tttccctgtc ctcagatcgt ttagcactgt ccaattcatc gtgcatttct gagagctggt
     5221 cctggagatc cctgatctca gtctggtgct gttcccgttc catcttcacc tggaacaatt
     5281 cctccaggtt cttgcggagc tccccttcac tctcttccag gcgctgctgt agcatggagt
     5341 tctcttccac tttactatca tgctggccct ggagctcctc caaatttgct ttcattctct
     5401 ctctctcttc tttgatgttc tgctgattct tgacttctag ttgaagctgt ctctgaagtt
     5461 cagccacttc tgtggtcagc ttgtttttct gttccagcag atctctgact tcagatgaag
     5521 aattagaagc ttgattattt ccttgtgtgc tccctgctgc tcgagacttt aaagtctgga
     5581 ttttctcaaa gaccaggttg actttccgtt tagtcgcatc atcattatcg gtgcttcctt
     5641 ctttgaggta attgtaaagt atctgcttgg ccgtctcctc attggtttgt tgagtgagct
     5701 cttgttggcc tttcaacaga tctggtgtgg cctgagtgtt ggaggcggaa gtaaatgtct
     5761 tcacagaaat cttcttggca ccagagtcgg gcgaagtcgc tagggcccga ttctgtaaca
     5821 tcagcgtcgc cgtggctgtc ttgatttcgt cctccttttt attctgggct gggggggagg
     5881 acacacgcgt gtttgtactc tcccgacccc ctctgtttgc tggagctttc ccatctttgc
     5941 tctgctgggc aggccggagg tgggccccct gcagcttggc gaaggcgcgg cccacgctct
     6001 gtctcccgcg gtgctcctgg gcaggtgacc gctgcgggca cacctgcgac gggcggagga
     6061 ggtgttcgct ggacttgccc aggttccggc taaattcgct gaggtactcc gagttcccct
     6121 gcagaccgaa aggaaaggca ctatccacgc ttctggacct cttcctgtct tctggattga
     6181 ttctgttccg cttcccaggc cttcctctcc tctgaagccc gggtttttgg tcaaactttt
     6241 caatcagttg atccacacca ggaattgatc ccgtgtcaat atcccgccca gttcctggca
     6301 ggaaggggat ataccgtctg ttttcgtggc gattcacatt gtcagcgtgg atggcacatt
     6361 cctgatcatc aagcagaaat ctgtacaaag agttggccga cgtgggggtt gccgacgagg
     6421 aagaggacct ccgggaccga gctccgtcca ggacaggtcc cgctgaatct tgtcgccgga
     6481 agggaagcac gtccggcctg gctttcttgg cttcaggatg ggtgtgcggg ctgtgggcag
     6541 tgggggacct cccactaggg gaaggggcct cccctaccag cgcctccccg gtgcagctct
     6601 caacatttac gcacactgag gtctgcttct tggggtcatc gatgaccaca gagctgcaca
     6661 aacggatggc cgtcacgccg gatttgcccg catcttccaa aggagggctg gtaggctggg
     6721 aactaccagg cttggggaag caggtccatg ccttcttatt gttggtacct tcttctgtca
     6781 atgtgtttag ccaattatgc tcaggaggct ggtggttttg caagttcgat tcattctttt
     6841 cagggtcata gggctgcaga agctctggat gcctctgaaa gttcaacacg tgggatggct
     6901 tcattggccc ctctttgcgt tctagaaccc cgttcttgcc ttcgaagaga gaggactgtt
     6961 tcaggtttct tactgggctg ggctgggcat acggattttc tgggagctgc aattcctctg
     7021 cgctgctctc ctccagcacg gacccgttgc tggaatgcag aggcaggtta cttctcattg
     7081 gagatggaaa tggtggccca ttttcaggaa aagatgtgcc tgccagacac cgctctgtgt
     7141 tattcaggac tatgtagggg tgaccatcga ttccctgcac ccgaatactg acaccatagg
     7201 agcctgcctt agagtgttgg gaattccttg gctttttgct atcatcgctt gcgagtctca
     7261 gatggacgcc atattcttgc tgtacgtgtt ggtattcgcc gaaatacagc tccatgcggc
     7321 cgctcttgta cagctcgtcc atgccgagag tgatcccggc ggcggtcacg aactccagca
     7381 ggaccatgtg atcgcgcttc tcgttggggt ctttgctcag ggcggactgg gtgctcaggt
     7441 agtggttgtc gggcagcagc acggggccgt cgccgatggg ggtgttctgc tggtagtggt
     7501 cggcgagctg cacgctgccg tcctcgatgt tgtggcggat cttgaagttc accttgatgc
     7561 cgttcttctg cttgtcggcc atgatataga cgttgtggct gttgtagttg tactccagct
     7621 tgtgccccag gatgttgccg tcctccttga agtcgatgcc cttcagctcg atgcggttca
     7681 ccagggtgtc gccctcgaac ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga
     7741 agaagatggt gcgctcctgg acgtagcctt cgggcatggc ggacttgaag aagtcgtgct
     7801 gcttcatgtg gtcggggtag cggctgaagc actgcacgcc gtaggtcagg gtggtcacga
     7861 gggtgggcca gggcacgggc agcttgccgg tggtgcagat gaacttcagg gtcagcttgc
     7921 cgtaggtggc atcgccctcg ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc
     7981 cgtccagctc gaccaggatg ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca
     8041 tggtggcgga tccactagtt ctagagggcc cgtttaaacg ctagccagct tgggtctccc
     8101 tatagtgagt cgtattaatt tcgataagcc agtaagcagt gggttctcta gttagccaga
     8161 gagctctgct tatatagacc tcccaccgta cacgcctacc gcccatttgc gtcaatgggg
     8221 cggagttgtt acgacatttt ggaaagtccc gttgattttg gtgccaaaac aaactcccat
     8281 tgacgtcaat ggggtggaga cttggaaatc cccgtgagtc aaaccgctat ccacgcccat
     8341 tgatgtactg ccaaaaccgc atcaccatgg taatagcgat gactaatacg tagatgtact
     8401 gccaagtagg aaagtcccat aaggtcatgt actgggcata atgccaggcg ggccatttac
     8461 cgtcattgac gtcaataggg ggcgtacttg gcatatgata cacttgatgt actgccaagt
     8521 gggcagttta ccgtaaatac tccacccatt gacgtcaatg gaaagtccct attggcgtta
     8581 ctatgggaac atacgtcatt attgacgtca atgggcgggg gtcgttgggc ggtcagccag
     8641 gcgggccatt taccgtaagt tatgtaacgc ggaactccat atatgggcta tgaactaatg
     8701 accccgtaat tgattactat taataactag tcaataatca atgtcaacgc gtatatctgg
     8761 cccgtacatc gcgaagcagc gcaaaacgcc taaccctaag cagattcttc atgcaattgt
     8821 cggtcaagcc ttgccttgtt gtagcttaaa ttttgctcgc gcactactca gcgacctcca
     8881 acacacaagc agggagcaga tactggctta actatgcggc atcagagcag attgtactga
     8941 gagtgcacca taggggatcg ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg
     9001 gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     9061 gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     9121 tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     9181 tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     9241 gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     9301 acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat
     9361 tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     9421 gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     9481 tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     9541 accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     9601 ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt
     9661 agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     9721 gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     9781 ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     9841 tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     9901 ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     9961 gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
    10021 acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
    10081 aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
    10141 atcttc
//
