LOCUS       sequence-469298-       11514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of NM2B (dog) - with HA and FLAG tags in mammalian 
            cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: HA-cNM2B-Flag
FEATURES             Location/Qualifiers
     source          1..11514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3278..3301)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(9326..9352)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9466..9485)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9467..9485)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(9530..9733)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(9559..9579)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        9734..10113
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     10285..10304
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        10379..10483
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10484..10552,10553..11344)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10702..10721)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcgggtttaa acgggccctc tagattactt gtcatcgtcg tccttgtaat
     3301 cctccgactg cggcggctgt gtctcgttga tgtcactggt cttactctcc gtgtcatcgt
     3361 ctgacagctc cagggaggct ccctcgatgt gtagctggcg ccggcccgat cggctggacg
     3421 agaagcttat ggggccacct cgtctcagcc ggttcttcag cgtgctgacc tcacggctca
     3481 ggccctcgtt ggcctccgtg gcatcgtcca gttcccgctg gagcttacgc cgagatgcgt
     3541 tggcacgtgt ggcttcctcc tctgcctcct ccagctgccg cttgagctgc ttcatcctgg
     3601 cgttggcctt ctccatctgt tctttatact gatccgcatg tcgacgctca tcttcaacct
     3661 gcatgaagat ctctttcagc tttttctccg tgcgacggac tagtttgttg gcggctgctc
     3721 gttccttggc ttcctgctcc agctgctcct ccagctgccc aatcttggct tccagggcag
     3781 agatggtggc cttgaacttg gacttgacgg cgccctccag ttcctgcagc ttggccttca
     3841 gctccttgtt ctgccgctct agctgctggc gtgcgttgtc gctcttctgg gcggcgctgc
     3901 gctcagccgc gagctcggcg ttcagcgtgt ccacctgcag cgtggtcttg cggaagcggt
     3961 cgttgagcag ctccatgttg ctctgctcct cttccagctc ctcctccagc tgcgcgatcc
     4021 gtgcctccag acgccgcttc tcatccagca gcgcagactt gccagaggcg ctgttggcga
     4081 tctcgtcagc cagctcatct ctctcctgct cggcgtggcg gcgcgctcgc tcagatgagg
     4141 ccagttcctc ctgcaattga aggatttctg cttccagact cttcagtttc ttttcactct
     4201 ctttggattg agcaaaaatc tcatcccggg atgcacgggc ttcttctaat tcacgttggt
     4261 aatccttcat ctgagcctga agcttgcgaa gctgcttgat cacttcgtcc ctcgctttgt
     4321 tcgcagcctc aatctgggct tcaaggtcct tcaggtctat ttccagtttc tttttagaag
     4381 cgaccgccag cgctcgctgc ttccgctcat cttccagctc tgcctccagc tcccgcacct
     4441 gcttaaccag gagccgcttc ttctcttcat tctgctcgtc cctggtttgt aagtccctct
     4501 caaactgggc cttcatggcc tgcatgttga cttccaggcg aagcttggcg tcttctgtgg
     4561 cctgaagctc atcctccagc tcttccagct gtgtcctcat ctcctccacc tgttgctcca
     4621 gggcccgctt cgatttttca agctcgtgca cgttcttccc cacgtcatcc ttggagctta
     4681 tcaggtcctc catgtctgcc cgcagctgct tgttctgtct ctcaaactct tccttggttt
     4741 ctagggcctc ctcgagggct cgggccaggg acagggcttt ggtttccttc tccctggcct
     4801 ctgcctctgc gcggtctcgc tcttctgcat agcgggcgga gatgttcttc tcctctgcca
     4861 gcagctggtc aaacttcttc tgcttcttct ccaagttgga ggcgatctgg cgctggtgat
     4921 ctaggtccac tgtcaggtcg tcgagctcct gctgcaggcg gttcttggtc ttctccagct
     4981 tgtcataggc cagagccttc tcctccaggc gctggcccag ggcctccaca tctttcagaa
     5041 gcttctttct ggcttcttct aagctctcga tggttcctaa gtcatcatct accttcttct
     5101 tggtatcggc gagctgggac tgcagcacca acacctgctt ttccaggttt ttcctggcct
     5161 cctcctcctc ctcctgctgc tcctggagac tgttcttctc ctcctccagc tgccggatgc
     5221 ggctgctcag gttcagcttc tggcgggtct cctcctgaag gagctcctga gtgtcctgta
     5281 gttgagactc aagactagct gcgtccttag caaacttaat gcccttcttc tctgcttctt
     5341 ccagaagact tgagacattg tccagttcat tctgtaattt gtttgccttc tcggccagct
     5401 ccaccctgag cctgtctcct tctgagacct tggcgtgcag ctcctgcacc tgggcgtcga
     5461 gcttcttcct cttgtgctca gactcagcct tgacctgctg tagcaccttc acctcacacg
     5521 ccagctcctt gttgtcagtc tccaggccct gcttgttctt ctccagattc gctttgaacc
     5581 tctttgcctg ttccagctgc tccgacagct cctccagggc tgtggcgtgt cgctgtctca
     5641 tgtcctggat ttgagcctcg tggctcctcg tttcctcctc cagagccttc ttcagctctg
     5701 ccacttcttg ttcacgcttc gtgcgaagtt cctgctgagc cgcggtggtg tccagcgtgt
     5761 cctccagctc tgttttcagg gcctccagct cctcactcag gtccctcttc tgcttctccg
     5821 ccttgttccg tgaagccttc tctgattcaa agtcctcctg aagctcggcg atttgggctt
     5881 gcagctctcg caccaccttg agggcattgt tcttatggag cgtctcatcg tcacccctgg
     5941 ccagcgcgcc ttgcagctcc tcctccttct tggccagctg gaccttgagc tcgtcgatct
     6001 gcgcctggag ctctgcgatc tggtcctgca ggtcagtggt ctcaccgtcc agttttcgtt
     6061 tggccttctc cagctcctga cgcgtcttct cttccttctt caagcgttct tctaaatctg
     6121 agatcatcac ttcttgctta ttcctgattt tggccaagtt tttggccttt tcttcctcct
     6181 cagccagctg agaggaacac tctgcaatgc gatcttccat gagtttcttt tctttgataa
     6241 atttggaatt ctggtcctca agcagcagga tctcctcctc catcttcttg atttttgctt
     6301 ccgctgtcac cttttccagc tgtagtttct ggcgagcgcc ttcttcctca tccagttgct
     6361 cctctaggtc ctgaatgtga gcttgcattt tcttcttttc attttggagg atttggtttc
     6421 gttcttcttc ctcttcgacc ctggattcca agtcatggag aatctcctcc agctcctgct
     6481 tcttggcggc gagcctggct ctcatctctt ctgcctcagc aaagagctct gtctctgctt
     6541 ggagctgctc tgttaggatg ttcttctctt ccaggagctg ctggtgcttc ctctccattt
     6601 cctccagctc gccctccacc ttcgtctgcc tctccttcac cttcagcagc tcctcgtctt
     6661 tggcctgaag ctcttcctcc tggcgggtca cttgcaggag cggcttcacc ttcgtgaaga
     6721 cacgccacca ctgccagtgc cggagcttca ggtatgcggc gcagttccgc tgcaggacct
     6781 tcagggcgct cagctgctgc tgcttcttgg cgaaggcctt tctggccagg tagcctctgc
     6841 agacagcctg gaagaagata atgatgtcgg tgatctttag atccctctct tcctctaagt
     6901 gtgccagcac gccagctcgg aaaaatatct tgctctgtcc gattctgtac aagtttgggt
     6961 ccagttctaa agcccggatc attcgttcgc aggcctgttt gccgtccatg aaacccttag
     7021 ggatagcatt tggagttagg atctcgtatc tctgtctgaa ttcctggaaa actatccggt
     7081 tggggaaccc ctggcgacag atcctgattc cttccagaac gccgttacag cgaagctgat
     7141 caagcactag gtgcggatcc agttttccag ctcgcttctc gtgatttgga atgatgcagc
     7201 ggacgaagtt agggttggtg ttccggagag ttgccatcag cttggtgagg gattctttgt
     7261 agagctgccc gacagtccga aacatgccct tcttggtttt atacgcagag ccaaaggctg
     7321 tctccgtcat gcccgtgacc tgatccagac ccacgatacg gtccactggt ggctcatgaa
     7381 gaccagaaac actgtcatag aaagaagctc tctgaatatt ctgaatctca tccttccaaa
     7441 gctctgccac gaatctgtca gatgactggt gcaggagggt ggccacattg tcgttcaggg
     7501 ggtccatgtt cttcatcagc cactcatcag ccttgtagtc caccttgccc gcataatgga
     7561 tgatgcagaa gtcagctctg tctttcagtt gccggggttt ctggaacttg gagtgagagc
     7621 cttgctcttg aactagtttt tcaacaaagg ttttgtccgt ggccttcggg aaccagcact
     7681 cttcatccaa aagggccagc acaccagggg ggttagcggg tctctcgatg aggtcgatgc
     7741 agggctgcag gtcgaggcca aagtcaatga agctccactc gatgccctcg cgctggtact
     7801 cctcctgctc caggatgaac atggtgtggt tgaagagctg ctgcagcttc tcattggtgt
     7861 agttgatgca cagttgctcg aacgagttga gctcaaatat ttcgaatcca gcgatatcca
     7921 ggattccaat aaaagatgcg ccctgacgct tggtcctgtc cagagctttg ttgatacggt
     7981 gaacgagcca acggaagagc cgctcatagg tagcttttgc caatgcttct accgcaaaat
     8041 ctgcttgttc tttggtctgg gctttctgca catagtctcg gccgaccttg atccgggggg
     8101 tgaggatggc ccgcgtgaac tccatcacgt tcatcccaag aagatggcag agtttctgtg
     8161 caactgtgtt ctctggcatg gatgcttgat cagtatttcg ctcctttttg aaagaaatat
     8221 ttccgaactg tagcactgat gacactactt taagcattga cagaatctcc tcgtgagaga
     8281 agcccatgat gtgcattgct tccatggttt cctgaaagtt gtccttgtct tgctgtcccg
     8341 ggatgggaat atagccgttg gagaggaatc tgtagttatt aaatccttcg aggagcaaat
     8401 ccgactttag gtgttctcct gctccagata acaactggta aaagatatga aaagtacgct
     8461 catcttttgc ttgacgaaca gcacgagatt tttccagaag gtatgtttca atgttggccc
     8521 caacaatata gccagttaca tcaaagttga ttcgaataaa cttgccaaag cgagatgaat
     8581 tatcattttt cacagtcttc gcatttccaa aggattccag gattggattt gcttgcaaaa
     8641 gctgccgttc aagttccccc tgatgtttca ctggtttagg cgattcagga atattatggt
     8701 ccttccgtcc tttatgcgaa gaagcaacat gagcaaggta ctgaatgact ttcttggtat
     8761 tttctgtctt cccagcacct gattcgcccg tgcaaagaat ggactggtcc tcacgatctt
     8821 gaagcatgca tctgtaagca gattcagata tagcatagat gtgtggaggc atctcgtggc
     8881 gtttcttccc tctgtacatt tcaataatat tctcagagta aattggaaga ttcttgtaag
     8941 gatttatgac tacacagaag agtccagagt aagtatagat caatcccgaa tagtagcggt
     9001 ccttcaggtt atgtagaaca gaagcttcgt tcaagcacgt caattccgcc atatcctcca
     9061 ccttggaaaa cttaggtggg ttcatcttct gaatatcgtc tttgttgacc atggctttct
     9121 tgccgttctc tgccaactcc accataacct catctccccg ctcctctttg atacttgctg
     9181 cctcgaaacc atggcgctct gatggaatcc acactagctt tttagccgtc cagtcggcct
     9241 gggtggcagg gttatagatg acagccctgt ccacaaagag gtacctctct gggtcttcaa
     9301 gtccagttct ctgggccata tcgatagcgt agtctgggac gtcgtatggg tacatggtgg
     9361 ccgcggccgc cactgtgctg gatatctgca gaattccacc acactggact agtggatccg
     9421 agctcggtac caagcttaag tttaaacgct agccagcttg ggtctcccta tagtgagtcg
     9481 tattaatttc gataagccag taagcagtgg gttctctagt tagccagaga gctctgctta
     9541 tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt caatggggcg gagttgttac
     9601 gacattttgg aaagtcccgt tgattttggt gccaaaacaa actcccattg acgtcaatgg
     9661 ggtggagact tggaaatccc cgtgagtcaa accgctatcc acgcccattg atgtactgcc
     9721 aaaaccgcat caccatggta atagcgatga ctaatacgta gatgtactgc caagtaggaa
     9781 agtcccataa ggtcatgtac tgggcataat gccaggcggg ccatttaccg tcattgacgt
     9841 caataggggg cgtacttggc atatgataca cttgatgtac tgccaagtgg gcagtttacc
     9901 gtaaatactc cacccattga cgtcaatgga aagtccctat tggcgttact atgggaacat
     9961 acgtcattat tgacgtcaat gggcgggggt cgttgggcgg tcagccaggc gggccattta
    10021 ccgtaagtta tgtaacgcgg aactccatat atgggctatg aactaatgac cccgtaattg
    10081 attactatta ataactagtc aataatcaat gtcaacgcgt atatctggcc cgtacatcgc
    10141 gaagcagcgc aaaacgccta accctaagca gattcttcat gcaattgtcg gtcaagcctt
    10201 gccttgttgt agcttaaatt ttgctcgcgc actactcagc gacctccaac acacaagcag
    10261 ggagcagata ctggcttaac tatgcggcat cagagcagat tgtactgaga gtgcaccata
    10321 ggggatcggg agatctcccg atccgtcgac gtcaggtggc acttttcggg gaaatgtgcg
    10381 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
    10441 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
    10501 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
    10561 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
    10621 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
    10681 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
    10741 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
    10801 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
    10861 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
    10921 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
    10981 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
    11041 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
    11101 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
    11161 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
    11221 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
    11281 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
    11341 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
    11401 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
    11461 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttc
//
