LOCUS       sequence-469299-        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Paracingulin (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  AUTHORS   Rouaud F, Huang W, Flinois A, Jain K, Vasileva E, Di Mattia T, 
            Mauperin M, Parry DAD, Dugina V, Chaponnier C, Mean I, Montessuit S,
            Mutero-Maeda A, Yan J, Citi S
  TITLE     Cingulin and paracingulin tether myosins-2 to junctions to 
            mechanoregulate the plasma membrane.
  JOURNAL   J Cell Biol. 2023 Jul 3;222(7):e202208065. doi: 
            10.1083/jcb.202208065. Epub 2023 May 19.
  PUBMED    37204781
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol."; date: "2023-07-3"; volume: "222(7):e202208065. doi"; pages: 
            " 10.1083/jcb.202208065. Epub 2023 May 19"
COMMENT     Sequence Label: GFP-mCGNL1
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(join(6774..7484,7485..7487,7488..7490))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6774..7484,7485..7487,7488..7490))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6816..6837)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7165..7184
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7424..7445
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7566..7585)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7567..7585)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7630..7833)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7659..7679)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7834..8213
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8385..8404
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8479..8583
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8584..8652,8653..9444)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8802..8821)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggccgc tcagatctgg
     2881 ctggcgatag tgctgtcttt gggaaaggca tagctcagtg gggcttcgta gaggcttccc
     2941 gcatcactgc tgaggtcgtc gtcgtccgag tcgtccagta ctttactaga cagtgtctta
     3001 agccttaagc ccttcttcag agagttgagc tgcccctgca gctgctcatt cacccccatc
     3061 tgctcctcca gctccctctg cagcttcttc ttagaacttt ccagtcggtc gatctcctct
     3121 tcagcctcct ccacctggcg cttcatggct ttcaggcgca agctcagctg gtccttctgg
     3181 tccgtcagtg acagatgctc atcatccacc tgcatcacca gctccttcac cttccgctcc
     3241 agccttcggt tgctgagctg caggttggcc cgatccctct cctcattctc cagccggtcc
     3301 tccagctctg caatcctggc ctccatctgc accaccaggc cctccttgct ggacctgtag
     3361 gaaccttcca ggtggataat ccggctcttt aagtctttgt tctgtctctc tagggaaatc
     3421 ttgtcacact ctaagtcttg cttggcagct ttctcctgaa gaagctcgct cctcatctgc
     3481 tccatctgtt ccctgctcca cgtgatcctc tccgacagca agtcagcgtt ggttctttct
     3541 tcttccaatt caatctccaa ttgggacacc ttgtcctcca tctgcttgat gagtcggctg
     3601 cggtcatcct tgaggtggct cttggcctcc agctcatact ccaggtcctt cagcgactgc
     3661 tccagaagct gccgttttgt gagcgcctcc tcctcagctc gctggtagtc ctgcaactct
     3721 tcctccttga ggcgtaactc atcctgcatc ttcgaggctg ccagtctggc cttttccact
     3781 tccaggcatt tctccttcag ttgtgtctgc atttccgcaa gctcccggcg gttcttctcc
     3841 ttatactcgc ccagctgctt ctgcagctcc aaagaggatg tgcgggaggc ctcagcaatg
     3901 tcggccatct ccttctgcag cttctcgatg gtcttgtcca ggtgccaccg ctcgctctcc
     3961 atctcgttct tcagctttcg cagctgctcc ttctgctccg cctcgtcccg gagtttctcc
     4021 aggagttcct tctgctcctg ggagacctgg ctcagctctc tccgagcttg ctccagctcc
     4081 ttctccagcg cccttctggc acatgtggct tctttttctt cctttctggc atgcacaagg
     4141 gcttcctcca gctgctgtac ttcactctca cactttcgga gtgtctcctt tgcttgggcc
     4201 tcgtcaccct tcacatcctc catctgcctc tgtagctggg ctgccctgcg ctccagctcc
     4261 gcaatcctcc cctgcagctt ctcattctcc tccttcagca ctttctcacg caggtcagcc
     4321 tccgcctggc tctgctctga ggagttgctc ctgctggcca ggacctcaac attcttggtt
     4381 gcctcctcca cactctccct gagggcctgc agctcagcat cgtactgttc cttcagctta
     4441 tccatctcct ggtcatggct ggaaacctcc tccttcaagg cgcccttcaa ggcagtcagc
     4501 tcccgctccc gcttcctcaa gagatcttcc tgttcctctt tggcaatgag cagatcttga
     4561 aggtcctgct tggcttgcag gagctcctca atcagggctc ccttctccct gtcctcggat
     4621 cgcttggtgc tgtctagctc atcatgcatt tccgacagct ggtcctgcag gtctctgatc
     4681 tcagtctggt gctgttcacg ctccatcttc acttggaaca gctcttccaa gctcttcctc
     4741 agctcgcctt cactttcctc cagccgtctc tgcagggtgg ccgtctcctc cacttggctc
     4801 tggtgccgga ctcgcagctc ttctaaatcc tctctcattc tttctcgctc ctccttgatg
     4861 ttctgttggt tcttcatctc cagttgcagt tgctgctgta gttcagacac ttctaggatc
     4921 aatttgttct tctgatccaa tagactgttg ccttcagagg gagaattcgg agcttgattg
     4981 cttccttggg cactccctgc tgctctggac ttcaaagtct ggattttctc aaagaccagg
     5041 ttcactttcc ttttggtggc atcctcattg tctgtgcctc cttctttgag gtaattgtaa
     5101 aggatctgct tggcggtctc ctcgttcgtt tgttgtgtga gctcctgctg gccctttaag
     5161 agatctggtg tggcctgagt gctggagtcg gaaggaaatg tcttcactga aattttcttg
     5221 gcaccagaat ccgatgtggc agccaccgcc cggttctgta acatcaacgt ggcagtggct
     5281 attttgattt cttcctcttt tttattcggt gctggcaggg agggggcaca tgcccccgtg
     5341 ctttcctgcc ttcctttgtt cagaactttc ccatctttgt tctgcagggg aggcttgggg
     5401 tgggcacctt gagcgccctg cagctttgcg ggcgggctgc tgggactgtg cttcccacgg
     5461 tgctcctggg ccactgacct ctgcgggaag acctgggatg gccgaaggag gtgttcgcta
     5521 gacttgccca ggttcctgct gaactcggtg aggtattctg tgtttccctg gagaccaaat
     5581 ggaaaggcac tatctacact tcgggatctt ttcctgtcgt cgggattgat cctgtttctc
     5641 ttcccagacc ttcctcttct ctgaaggcca ggcttttggt caaatttttc aattaactgg
     5701 tccacaccag gaattgagca ggtgtcgata tcccgcccag ttcctggcaa gaagggaata
     5761 taccttctgt tttcatggcg gttgacgctg tcggcatgaa tggcgcattc ctgatcatca
     5821 agtaaaaatt tgtacaagga agtggcagaa gtaggagtcg tggatgacga agaggacctg
     5881 cgtgaccgag ctccatccag gatgggtcct gcggaatctt gtcgccggaa gggtagcaca
     5941 tctggcctgt ttttcttggt ttcagaatag gcctgtgggc tgggggcagt tggggatttc
     6001 cgtcgagggg aaagggtctc ctctcccacc ccctccttgg cgcacctctg aacgttcacg
     6061 cacactgagg tctgcttttg ggggtcttct atgaccacag agctgcagag acggatcgct
     6121 gtcatgttgg tcttggctaa atcttccgaa gtagggctgt tgggctggga accgtaggat
     6181 ttggggaagc agttccggga cttcttacag ttggtcccgt cctctgttgc atttctcagc
     6241 cagggactct cgggagggtg atgcttcttt gcgttcacct cattcttttc tgggtcatag
     6301 ggctgcagaa gctccgggtg cctctggaag ttgagcacat ggggcagctt cgtgggcccg
     6361 tctttgcgtt ctagaactcc attcctgccc tcgtggaggg agaactgctt ctggcctctt
     6421 agcgggctgg tttgcaggta cgggttttct ggaagctgca gttcctccgg agtgttctcc
     6481 ttcaacacgg ttccattgct tggatgtagg gacaggttat ttatcactga agatggaaac
     6541 gatggcgcgt tttctgggaa aggtgtgcct gctagacacc gctcagtgtt attcaggact
     6601 atataagggt ggccatcaat tccctggacc ctgatgctga caccatagga gcctgccttt
     6661 gagggctggg aattccttgg ctttggagtg tcaccactgg caagtctcag gtgtaccccg
     6721 tactcctgct gtacgtgttg gtattcgcca aaatacagct ccatgcggcc gctcttgtac
     6781 agctcgtcca tgccgagagt gatcccggcg gcggtcacga actccagcag gaccatgtga
     6841 tcgcgcttct cgttggggtc tttgctcagg gcggactggg tgctcaggta gtggttgtcg
     6901 ggcagcagca cggggccgtc gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc
     6961 acgctgccgt cctcgatgtt gtggcggatc ttgaagttca ccttgatgcc gttcttctgc
     7021 ttgtcggcca tgatatagac gttgtggctg ttgtagttgt actccagctt gtgccccagg
     7081 atgttgccgt cctccttgaa gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg
     7141 ccctcgaact tcacctcggc gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg
     7201 cgctcctgga cgtagccttc gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg
     7261 tcggggtagc ggctgaagca ctgcacgccg taggtcaggg tggtcacgag ggtgggccag
     7321 ggcacgggca gcttgccggt ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca
     7381 tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg
     7441 accaggatgg gcaccacccc ggtgaacagc tcctcgccct tgctcaccat gaattccacc
     7501 acactggact agtggatccg agctcggtac caagcttaag tttaaacgct agccagcttg
     7561 ggtctcccta tagtgagtcg tattaatttc gataagccag taagcagtgg gttctctagt
     7621 tagccagaga gctctgctta tatagacctc ccaccgtaca cgcctaccgc ccatttgcgt
     7681 caatggggcg gagttgttac gacattttgg aaagtcccgt tgattttggt gccaaaacaa
     7741 actcccattg acgtcaatgg ggtggagact tggaaatccc cgtgagtcaa accgctatcc
     7801 acgcccattg atgtactgcc aaaaccgcat caccatggta atagcgatga ctaatacgta
     7861 gatgtactgc caagtaggaa agtcccataa ggtcatgtac tgggcataat gccaggcggg
     7921 ccatttaccg tcattgacgt caataggggg cgtacttggc atatgataca cttgatgtac
     7981 tgccaagtgg gcagtttacc gtaaatactc cacccattga cgtcaatgga aagtccctat
     8041 tggcgttact atgggaacat acgtcattat tgacgtcaat gggcgggggt cgttgggcgg
     8101 tcagccaggc gggccattta ccgtaagtta tgtaacgcgg aactccatat atgggctatg
     8161 aactaatgac cccgtaattg attactatta ataactagtc aataatcaat gtcaacgcgt
     8221 atatctggcc cgtacatcgc gaagcagcgc aaaacgccta accctaagca gattcttcat
     8281 gcaattgtcg gtcaagcctt gccttgttgt agcttaaatt ttgctcgcgc actactcagc
     8341 gacctccaac acacaagcag ggagcagata ctggcttaac tatgcggcat cagagcagat
     8401 tgtactgaga gtgcaccata ggggatcggg agatctcccg atccgtcgac gtcaggtggc
     8461 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     8521 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
     8581 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
     8641 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
     8701 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
     8761 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
     8821 tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
     8881 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
     8941 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
     9001 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
     9061 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
     9121 atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
     9181 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
     9241 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
     9301 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
     9361 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
     9421 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
     9481 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
     9541 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
     9601 atcaaaggat cttc
//
