LOCUS       sequence-469302-        9837 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Cingulin (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9837)
  AUTHORS   Vasileva E, Spadaro D, Rouaud F, King JM, Flinois A, Shah J, 
            Sluysmans S, Mean I, Jond L, Turner JR, Citi S
  TITLE     Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to 
            promote its extended conformation, stabilization, and tight junction
            accumulation.
  JOURNAL   J Biol Chem. 2022 Apr;298(4):101797. doi: 10.1016/j.jbc.2022.101797.
            Epub 2022 Mar 5.
  PUBMED    35259394
REFERENCE   2  (bases 1 to 9837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-04"; volume: "298(4):101797. doi"; pages: " 
            10.1016/j.jbc.2022.101797. Epub 2022 Mar 5"
COMMENT     Sequence Label: GFP-mCGN-Myc
FEATURES             Location/Qualifiers
     source          1..9837
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(1..3,4..6,7..717)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(46..67)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(307..326)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     654..675
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4309..4338
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4384..4413
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             4429..4446
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(4490..4507)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    4496..4720
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      4766..5194
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4853..4872)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5063..5084
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5203..5223)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        5208..5537
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      5388..5523
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     5450..5469
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             5604..6398
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(5658..5677)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     6268..6287
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    6572..6693
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6609..6628)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6663..6682
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6742..6758)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6742..6758)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(6755..6777)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    6766..6782
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(6790..6796,6797..6814,6815..6820))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6835..6856
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(6973..6990)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7144..7732)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(7224..7243)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7903..8694,8695..8763))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8526..8545
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8764..8868)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(8943..8962)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        9134..9513
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        9514..9717
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     9668..9688
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     9762..9781
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        9762..9780
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     9814..9830
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     9814..9830
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     regulatory      join(9832..9837,1..4)
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt cgagctggac
       61 ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga tgccacctac
      121 ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc ctggcccacc
      181 ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga ccacatgaag
      241 cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg caccatcttc
      301 ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg cgacaccctg
      361 gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat cctggggcac
      421 aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa gcagaagaac
      481 ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt gcagctcgcc
      541 gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc cgacaaccac
      601 tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga tcacatggtc
      661 ctgctggagt tcgtgaccgc cgccgggatc actctcggca tggacgagct gtacaagagc
      721 ggccgcatga tggctgatcc ccggggcccc gtagaccatg gagttcaaat tcgatttatt
      781 acagagccag agggtgctac tgaaatgggc acccttcgtc gtagtgggcg gcgccctgca
      841 agggatgcaa gagccagtac ctatggggtc gctgtgcgtg tgcagggcat cgccgggcaa
      901 ccgtttgtgg ttcttaacag tggagagaaa gggactgact cctttggggt ccagatcaaa
      961 gggggcaaca accgggggtc cccaggtgca ctgagctccg attcagagct ccccgaaaac
     1021 ccctactctc aagtcaaggg acgtcctgcc acttctcgga gcagcacatc tgatgaggag
     1081 cccaaggacc acttgaatgg aaaactgatc cgttcccagt cgcaagcctc tctgaccggg
     1141 cttgccttta tgagtcctag taataggagc accagcttgc tggaactagc ccctaaaccg
     1201 acttcctcga ttaacaccat tgacactgcc cccttgtctt cagtggactc gctcatcaac
     1261 aagtttgata gtcagaaggg ggggcaggtc cggggtcgca ctgggcgccg cacaaggaca
     1321 cttccccacg aacagcgcaa gcggagccag agtctggaca gcaggctccc acgggataca
     1381 cgtgaggaac gggagcacca gtccgctaac cactggaccc gcggcacaaa gtatgacaac
     1441 catgtggaca gttcaaagaa cccatcgcag aagcagagcc cgttcagcag cttcagccgt
     1501 tcgcgtcaga ctcaagactg ggtgcttcag agttttgagg agacacggga tccagccatg
     1561 gtgcagttca aatcaactcc agaccttctc agagaccaga gagagacagc gccaccaggc
     1621 agtgcggacc atgtgaaggc caccatctat ggcatcctaa gggagggaag ctcggagagt
     1681 gaggcatctg tgaggaggaa agttagtttg gtgctggagc aaatgcagcc tctggggatg
     1741 gtttctcctg cttctaccaa ggccctggca ggacaggctg agcttacccg aaaaatggag
     1801 gaacttcaga agaagctgga tgaggaggtg aagaagcggc agaagctaga accatccagg
     1861 gttgggctgg agaggcagct ggaggagaag gctgaagagt gccaccgact gcaggagttg
     1921 ctggagagga gaaaggggga ggtccagcag agcagcaagg aacttcagaa catgaagctc
     1981 ctgctaggcc aggaagaagg actacggcat gggctggagg cccaggtgaa ggagctacag
     2041 cttaagctca agcatagcca gagtcccgat tctggaaagg aaagtctcct aaaggacttg
     2101 ttagacaccc gggaacttct agaagaactc ttagagggca aacaacgagt agaggagcag
     2161 ctcaggctga gggaacggga gctgacagcc ctgaaggggg ccctgaaaga ggaggtggcc
     2221 tcccacgacc aggaggtgga acatgtccgg ctgcagtacc agcgagacac ggagcagctt
     2281 cgccggagca tgcaagatgc gacccaggac catgctgcac tggaggctga gaggcagaag
     2341 atgtcttccc tcgtgcggga gctgcaaaga gagctagagg agacctctga ggagacaggg
     2401 cattggcaga gcatgttcca gaagaacaag gaagagctga gagccaccaa gcaggaactg
     2461 ctgcaactgc ggatggagaa ggaggagatg gaagaggaac ttggggagaa gatggaggtc
     2521 ttacagaggg acttagagca ggcccgagcc agtacaagag atactcacca ggttgaggaa
     2581 cttaaaaagg agcttcgacg cacccagggg gagcttaaag agcttcaggc agagcagcag
     2641 aaccaggagg tgactgggcg acaccagaac caggtgttag agaagcagct ggcagcgctg
     2701 agggaggagg ctgatcgagg ccgagagctg gagcagcaga acctccagct acaaaagacc
     2761 ctgcagcaac tgcggcagga ctgtgaagag gcttccaagg ctaaggtggc atcagagaca
     2821 gaggccatgg tgctggggca gcgtcgggca accgtggaaa ccacacttcg ggagacacaa
     2881 gaggaaaatg acgaattccg aaggcgcatc ctgggtctgg agcagcagtt gaaggaggcc
     2941 cgaggtctgg cggaaggtgg ggaagcggtg gaggcacggc ttcgggacaa agtgcatcgg
     3001 ctggaggtag agaaacagca gctggaagag gctttgaatg ctgcccggga ggaggagggg
     3061 aacctggcag cagccaagcg ggctctggag gtccggctag atgaggccca gcgggggctg
     3121 gcacgccttg ggcaggaaca gcaagcactg aaccgggccc tagaggaaga ggggaagcag
     3181 cgggaggctc tccggaggag caaggcagag ttggaggagc agaagcgttt gctgaacagg
     3241 actgtagacc gactcaacaa agagctggag cagattgggg atgactccaa gctagccctg
     3301 cagcagctcc aggctcagat ggaagattac aaggaaaagg cccggaagga agtagctgat
     3361 gcccagcgtc aggccaagga ttgggccagt gaggctgaaa agaattcagg ggggctgagc
     3421 cggcttcagg atgagctgca aaggctgcgg caagccctgc agacatccca ggctgagcgg
     3481 gacacagcca ggctggacaa agagctgctg gcccagcgac tgcagggtct agagcaagag
     3541 gctgagaaca agaaacgctt ccaggacgac aaggctcgac agctgaagag cctagaggag
     3601 aaagtctcgc ggctggaagc ggagttagat gaagaaaaga acactgtgga actgttaaca
     3661 gatcgggtga accgtggccg ggatcaggtg gatcaactga ggacagagct catgcaggag
     3721 agatctgctc gacaggactt ggagtgtgac aaaatctcct tggagagaca gaacaaggat
     3781 ttgaagaccc ggttggccag ttcagagggc ttccagaaac ccagcgctag cctctcccag
     3841 cttgagtccc agaatcagct gctgcaagag cggctgcaag ctgaagagag agagaagaca
     3901 gttctgcagt ccaccaatcg caaactagaa cggagggtta aggagctctc catccagatt
     3961 gatgatgaac ggcagcatgt caacgaccag aaggaccagc taaccctgag ggtgaaggct
     4021 ttgaagcggc aggtggatga agcagaagag gaaattgagc ggctggacag cctgaggaag
     4081 aaggcacagc gggaactgga ggaacagcat gaggtcaatg aacagctcca agcccggatc
     4141 aaatccttgg aaaaggatgc ctggcggaaa gcttcccgct cagctgctga gtcagccctc
     4201 aaacaagagg ggctgagctc tgatgaggag tttgacaacg tctacgaccc ctcatccatt
     4261 gcatcactgc ttactgagag caacctgcag actagctcat gtatcgatga acaaaaactc
     4321 atctcagaag aggatctgaa ttaggtcgac ctcgaggggg ggcccggtac caagcttggg
     4381 cccgaacaaa aactcatctc agaagaggat ctgaatagcg ccgtcgacca tcatcatcat
     4441 catcattgag tttaaacggt ctccagctta agtttaaacc gctgatcagc ctcgactgtg
     4501 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     4561 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     4621 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     4681 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     4741 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4801 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4861 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4921 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4981 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     5041 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5101 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5161 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     5221 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     5281 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     5341 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     5401 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     5461 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     5521 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     5581 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     5641 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     5701 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     5761 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     5821 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     5881 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     5941 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     6001 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     6061 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     6121 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     6181 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     6241 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     6301 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     6361 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     6421 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     6481 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     6541 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     6601 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     6661 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     6721 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     6781 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     6841 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     6901 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     6961 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     7021 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     7081 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     7141 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     7201 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     7261 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     7321 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     7381 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     7441 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     7501 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     7561 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     7621 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     7681 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
     7741 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
     7801 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
     7861 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
     7921 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
     7981 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
     8041 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
     8101 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
     8161 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
     8221 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
     8281 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
     8341 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
     8401 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
     8461 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
     8521 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
     8581 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
     8641 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
     8701 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
     8761 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
     8821 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
     8881 aaaagtgcca cctgacgtcg acggatcggg agatctcccg atcccctatg gtgcactctc
     8941 agtacaatct gctctgatgc cgcatagtta agccagtatc tgctccctgc ttgtgtgttg
     9001 gaggtcgctg agtagtgcgc gagcaaaatt taagctacaa caaggcaagg cttgaccgac
     9061 aattgcatga agaatctgct tagggttagg cgttttgcgc tgcttcgcga tgtacgggcc
     9121 agatatacgc gttgacattg attattgact agttattaat agtaatcaat tacggggtca
     9181 ttagttcata gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct
     9241 ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta
     9301 acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac
     9361 ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt
     9421 aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag
     9481 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
     9541 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
     9601 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
     9661 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctctc
     9721 tggctaacta gagaacccac tgcttactgg cttatcgaaa ttaatacgac tcactatagg
     9781 gagacccaag ctggctagcg tttaaacggg ccctctagaa ctagtggatc cgccacc
//
