LOCUS       sequence-469303-       10155 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Paracingulin (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10155)
  AUTHORS   Vasileva E, Spadaro D, Rouaud F, King JM, Flinois A, Shah J, 
            Sluysmans S, Mean I, Jond L, Turner JR, Citi S
  TITLE     Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to 
            promote its extended conformation, stabilization, and tight junction
            accumulation.
  JOURNAL   J Biol Chem. 2022 Apr;298(4):101797. doi: 10.1016/j.jbc.2022.101797.
            Epub 2022 Mar 5.
  PUBMED    35259394
REFERENCE   2  (bases 1 to 10155)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-04"; volume: "298(4):101797. doi"; pages: " 
            10.1016/j.jbc.2022.101797. Epub 2022 Mar 5"
COMMENT     Sequence Label: GFP-mCGNL1-Myc
FEATURES             Location/Qualifiers
     source          1..10155
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..3304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3320..3349)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(3395..3424)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(7334..8044,8045..8047,8048..8050))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(7334..8044,8045..8047,8048..8050))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7376..7397)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7725..7744
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7984..8005
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      8047..8056
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(8058..8074)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8058..8074)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8107..8126)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8108..8126)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(8171..8374)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(8200..8220)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8375..8754
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8926..8945
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        9020..9124
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9125..9193,9194..9985)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9343..9362)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccggg ccccccctcg aggtcgacct aattcagatc ctcttctgag atgagttttt
     3421 gttcatcgat tcagatctgg ctggcgatag tgctgtcttt gggaaaggca tagctcagtg
     3481 gggcttcgta gaggcttccc gcatcactgc tgaggtcgtc gtcgtccgag tcgtccagta
     3541 ctttactaga cagtgtctta agccttaagc ccttcttcag agagttgagc tgcccctgca
     3601 gctgctcatt cacccccatc tgctcctcca gctccctctg cagcttcttc ttagaacttt
     3661 ccagtcggtc gatctcctct tcagcctcct ccacctggcg cttcatggct ttcaggcgca
     3721 agctcagctg gtccttctgg tccgtcagtg acagatgctc atcatccacc tgcatcacca
     3781 gctccttcac cttccgctcc agccttcggt tgctgagctg caggttggcc cgatccctct
     3841 cctcattctc cagccggtcc tccagctctg caatcctggc ctccatctgc accaccaggc
     3901 cctccttgct ggacctgtag gaaccttcca ggtggataat ccggctcttt aagtctttgt
     3961 tctgtctctc tagggaaatc ttgtcacact ctaagtcttg cttggcagct ttctcctgaa
     4021 gaagctcgct cctcatctgc tccatctgtt ccctgctcca cgtgatcctc tccgacagca
     4081 agtcagcgtt ggttctttct tcttccaatt caatctccaa ttgggacacc ttgtcctcca
     4141 tctgcttgat gagtcggctg cggtcatcct tgaggtggct cttggcctcc agctcatact
     4201 ccaggtcctt cagcgactgc tccagaagct gccgttttgt gagcgcctcc tcctcagctc
     4261 gctggtagtc ctgcaactct tcctccttga ggcgtaactc atcctgcatc ttcgaggctg
     4321 ccagtctggc cttttccact tccaggcatt tctccttcag ttgtgtctgc atttccgcaa
     4381 gctcccggcg gttcttctcc ttatactcgc ccagctgctt ctgcagctcc aaagaggatg
     4441 tgcgggaggc ctcagcaatg tcggccatct ccttctgcag cttctcgatg gtcttgtcca
     4501 ggtgccaccg ctcgctctcc atctcgttct tcagctttcg cagctgctcc ttctgctccg
     4561 cctcgtcccg gagtttctcc aggagttcct tctgctcctg ggagacctgg ctcagctctc
     4621 tccgagcttg ctccagctcc ttctccagcg cccttctggc acatgtggct tctttttctt
     4681 cctttctggc atgcacaagg gcttcctcca gctgctgtac ttcactctca cactttcgga
     4741 gtgtctcctt tgcttgggcc tcgtcaccct tcacatcctc catctgcctc tgtagctggg
     4801 ctgccctgcg ctccagctcc gcaatcctcc cctgcagctt ctcattctcc tccttcagca
     4861 ctttctcacg caggtcagcc tccgcctggc tctgctctga ggagttgctc ctgctggcca
     4921 ggacctcaac attcttggtt gcctcctcca cactctccct gagggcctgc agctcagcat
     4981 cgtactgttc cttcagctta tccatctcct ggtcatggct ggaaacctcc tccttcaagg
     5041 cgcccttcaa ggcagtcagc tcccgctccc gcttcctcaa gagatcttcc tgttcctctt
     5101 tggcaatgag cagatcttga aggtcctgct tggcttgcag gagctcctca atcagggctc
     5161 ccttctccct gtcctcggat cgcttggtgc tgtctagctc atcatgcatt tccgacagct
     5221 ggtcctgcag gtctctgatc tcagtctggt gctgttcacg ctccatcttc acttggaaca
     5281 gctcttccaa gctcttcctc agctcgcctt cactttcctc cagccgtctc tgcagggtgg
     5341 ccgtctcctc cacttggctc tggtgccgga ctcgcagctc ttctaaatcc tctctcattc
     5401 tttctcgctc ctccttgatg ttctgttggt tcttcatctc cagttgcagt tgctgctgta
     5461 gttcagacac ttctaggatc aatttgttct tctgatccaa tagactgttg ccttcagagg
     5521 gagaattcgg agcttgattg cttccttggg cactccctgc tgctctggac ttcaaagtct
     5581 ggattttctc aaagaccagg ttcactttcc ttttggtggc atcctcattg tctgtgcctc
     5641 cttctttgag gtaattgtaa aggatctgct tggcggtctc ctcgttcgtt tgttgtgtga
     5701 gctcctgctg gccctttaag agatctggtg tggcctgagt gctggagtcg gaaggaaatg
     5761 tcttcactga aattttcttg gcaccagaat ccgatgtggc agccaccgcc cggttctgta
     5821 acatcaacgt ggcagtggct attttgattt cttcctcttt tttattcggt gctggcaggg
     5881 agggggcaca tgcccccgtg ctttcctgcc ttcctttgtt cagaactttc ccatctttgt
     5941 tctgcagggg aggcttgggg tgggcacctt gagcgccctg cagctttgcg ggcgggctgc
     6001 tgggactgtg cttcccacgg tgctcctggg ccactgacct ctgcgggaag acctgggatg
     6061 gccgaaggag gtgttcgcta gacttgccca ggttcctgct gaactcggtg aggtattctg
     6121 tgtttccctg gagaccaaat ggaaaggcac tatctacact tcgggatctt ttcctgtcgt
     6181 cgggattgat cctgtttctc ttcccagacc ttcctcttct ctgaaggcca ggcttttggt
     6241 caaatttttc aattaactgg tccacaccag gaattgagca ggtgtcgata tcccgcccag
     6301 ttcctggcaa gaagggaata taccttctgt tttcatggcg gttgacgctg tcggcatgaa
     6361 tggcgcattc ctgatcatca agtaaaaatt tgtacaagga agtggcagaa gtaggagtcg
     6421 tggatgacga agaggacctg cgtgaccgag ctccatccag gatgggtcct gcggaatctt
     6481 gtcgccggaa gggtagcaca tctggcctgt ttttcttggt ttcagaatag gcctgtgggc
     6541 tgggggcagt tggggatttc cgtcgagggg aaagggtctc ctctcccacc ccctccttgg
     6601 cgcacctctg aacgttcacg cacactgagg tctgcttttg ggggtcttct atgaccacag
     6661 agctgcagag acggatcgct gtcatgttgg tcttggctaa atcttccgaa gtagggctgt
     6721 tgggctggga accgtaggat ttggggaagc agttccggga cttcttacag ttggtcccgt
     6781 cctctgttgc atttctcagc cagggactct cgggagggtg atgcttcttt gcgttcacct
     6841 cattcttttc tgggtcatag ggctgcagaa gctccgggtg cctctggaag ttgagcacat
     6901 ggggcagctt cgtgggcccg tctttgcgtt ctagaactcc attcctgccc tcgtggaggg
     6961 agaactgctt ctggcctctt agcgggctgg tttgcaggta cgggttttct ggaagctgca
     7021 gttcctccgg agtgttctcc ttcaacacgg ttccattgct tggatgtagg gacaggttat
     7081 ttatcactga agatggaaac gatggcgcgt tttctgggaa aggtgtgcct gctagacacc
     7141 gctcagtgtt attcaggact atataagggt ggccatcaat tccctggacc ctgatgctga
     7201 caccatagga gcctgccttt gagggctggg aattccttgg ctttggagtg tcaccactgg
     7261 caagtctcag gtgtaccccg tactcctgct gtacgtgttg gtattcgcca aaatacagct
     7321 ccatgcggcc gctcttgtac agctcgtcca tgccgagagt gatcccggcg gcggtcacga
     7381 actccagcag gaccatgtga tcgcgcttct cgttggggtc tttgctcagg gcggactggg
     7441 tgctcaggta gtggttgtcg ggcagcagca cggggccgtc gccgatgggg gtgttctgct
     7501 ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt gtggcggatc ttgaagttca
     7561 ccttgatgcc gttcttctgc ttgtcggcca tgatatagac gttgtggctg ttgtagttgt
     7621 actccagctt gtgccccagg atgttgccgt cctccttgaa gtcgatgccc ttcagctcga
     7681 tgcggttcac cagggtgtcg ccctcgaact tcacctcggc gcgggtcttg tagttgccgt
     7741 cgtccttgaa gaagatggtg cgctcctgga cgtagccttc gggcatggcg gacttgaaga
     7801 agtcgtgctg cttcatgtgg tcggggtagc ggctgaagca ctgcacgccg taggtcaggg
     7861 tggtcacgag ggtgggccag ggcacgggca gcttgccggt ggtgcagatg aacttcaggg
     7921 tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga cacgctgaac ttgtggccgt
     7981 ttacgtcgcc gtccagctcg accaggatgg gcaccacccc ggtgaacagc tcctcgccct
     8041 tgctcaccat ggtggcggat ccactagttc tagagggccc gtttaaacgc tagccagctt
     8101 gggtctccct atagtgagtc gtattaattt cgataagcca gtaagcagtg ggttctctag
     8161 ttagccagag agctctgctt atatagacct cccaccgtac acgcctaccg cccatttgcg
     8221 tcaatggggc ggagttgtta cgacattttg gaaagtcccg ttgattttgg tgccaaaaca
     8281 aactcccatt gacgtcaatg gggtggagac ttggaaatcc ccgtgagtca aaccgctatc
     8341 cacgcccatt gatgtactgc caaaaccgca tcaccatggt aatagcgatg actaatacgt
     8401 agatgtactg ccaagtagga aagtcccata aggtcatgta ctgggcataa tgccaggcgg
     8461 gccatttacc gtcattgacg tcaatagggg gcgtacttgg catatgatac acttgatgta
     8521 ctgccaagtg ggcagtttac cgtaaatact ccacccattg acgtcaatgg aaagtcccta
     8581 ttggcgttac tatgggaaca tacgtcatta ttgacgtcaa tgggcggggg tcgttgggcg
     8641 gtcagccagg cgggccattt accgtaagtt atgtaacgcg gaactccata tatgggctat
     8701 gaactaatga ccccgtaatt gattactatt aataactagt caataatcaa tgtcaacgcg
     8761 tatatctggc ccgtacatcg cgaagcagcg caaaacgcct aaccctaagc agattcttca
     8821 tgcaattgtc ggtcaagcct tgccttgttg tagcttaaat tttgctcgcg cactactcag
     8881 cgacctccaa cacacaagca gggagcagat actggcttaa ctatgcggca tcagagcaga
     8941 ttgtactgag agtgcaccat aggggatcgg gagatctccc gatccgtcga cgtcaggtgg
     9001 cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa
     9061 tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa
     9121 gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct
     9181 tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg
     9241 tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg
     9301 ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt
     9361 atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga
     9421 cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga
     9481 attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac
     9541 gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg
     9601 ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac
     9661 gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct
     9721 agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct
     9781 gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg
     9841 gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat
     9901 ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg
     9961 tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat
    10021 tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct
    10081 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa
    10141 gatcaaagga tcttc
//
