LOCUS       sequence-469304-        9338 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of GFP-Cingulin (mouse) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9338)
  AUTHORS   Flinois A, Mean I, Mutero-Maeda A, Guillemot L, Citi S
  TITLE     Paracingulin recruits CAMSAP3 to tight junctions and regulates 
            microtubule and polarized epithelial cell organization.
  JOURNAL   J Cell Sci. 2024 Mar 1;137(5):jcs260745. doi: 10.1242/jcs.260745. 
            Epub 2023 May 15.
  PUBMED    37013686
REFERENCE   2  (bases 1 to 9338)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2024-03-1"; volume: "137(5):jcs260745. doi"; pages: " 
            10.1242/jcs.260745. Epub 2023 May 15"
COMMENT     Sequence Label: GFP-mCGN
FEATURES             Location/Qualifiers
     source          1..9338
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1291..1665)
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     promoter        complement(1684..1731)
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     promoter        complement(1779..2108)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1793..1928
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1847..1866)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2093..2113
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2122..2550)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2232..2253)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2444..2463
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    2596..2820
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     2809..2826
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(2877..2906)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(join(6498..7208,7209..7211,7212..7214))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6498..7208,7209..7211,7212..7214))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(6540..6561)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     6889..6908
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7148..7169
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7290..7309)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7291..7309)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7354..7557)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7383..7403)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        7558..7937
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8109..8128
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8203..8307
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8308..8376,8377..9168)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8526..8545)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtagcacgtg tcagtcctgc tcctcggcca cgaagtgcac
     1321 gcagttgccg gccgggtcgc gcagggcgaa ctcccgcccc cacggctgct cgccgatctc
     1381 ggtcatggcc ggcccggagg cgtcccggaa gttcgtggac acgacctccg accactcggc
     1441 gtacagctcg tccaggccgc gcacccacac ccaggccagg gtgttgtccg gcaccacctg
     1501 gtcctggacc gcgctgatga acagggtcac gtcgtcccgg accacaccgg cgaagtcgtc
     1561 ctccacgaag tcccgggaga acccgagccg gtcggtccag aactcgaccg ctccggcgac
     1621 gtcgcgcgcg gtgagcaccg gaacggcact ggtcaacttg gccatggttt agttcctcac
     1681 cttgtcgtat tatactatgc cgatatacta tgccgatgat taattgtcaa cacgtgctga
     1741 tcagatccga aaatggatat acaagctccc gggagctttt tgcaaaagcc taggcctcca
     1801 aaaaagcctc ctcactactt ctggaatagc tcagaggcag aggcggcctc ggcctctgca
     1861 taaataaaaa aaattagtca gccatggggc ggagaatggg cggaactggg cggagttagg
     1921 ggcgggatgg gcggagttag gggcgggact atggttgctg actaattgag atgcatgctt
     1981 tgcatacttc tgcctgctgg ggagcctggg gactttccac acctggttgc tgactaattg
     2041 agatgcatgc tttgcatact tctgcctgct ggggagcctg gggactttcc acaccctaac
     2101 tgacacacat tccacagaat taattcgcgt taaatttttg ttaaatcagc tcatttttta
     2161 accaataggc cgaaatcggc aaaatccctt ataaatcaaa agaatagacc gagatagggt
     2221 tgagtgttgt tccagtttgg aacaagagtc cactattaaa gaacgtggac tccaacgtca
     2281 aagggcgaaa aaccgtctat cagggcgatg gcccactacg tgaaccatca ccctaatcaa
     2341 gttttttggg gtcgaggtgc cgtaaagcac taaatcggaa ccctaaaggg agcccccgat
     2401 ttagagcttg acggggaaag ccggcgaacg tggcgagaaa ggaagggaag aaagcgaaag
     2461 gagcgggcgc tagggcgctg gcaagtgtag cggtcacgct gcgcgtaacc accacacccg
     2521 ccgcgcttaa tgcgccgcta cagggcgcgt ggggataccc cctagagccc cagctggttc
     2581 tttccgcctc agaagccata gagcccaccg catccccagc atgcctgcta ttgtcttccc
     2641 aatcctcccc cttgctgtcc tgccccaccc caccccccag aatagaatga cacctactca
     2701 gacaatgcga tgcaatttcc tcattttatt aggaaaggac agtgggagtg gcaccttcca
     2761 gggtcaagga aggcacgggg gaggggcaaa caacagatgg ctggcaacta gaaggcacag
     2821 tcgaggctga tcagcgggtt taaacgggcc ctctagactc gagcggccgc ctaattcaga
     2881 tcctcttctg agatgagttt ttgttcatcg atacatgagc tagtctgcag gttgctctca
     2941 gtaagcagtg atgcaatgga tgaggggtcg tagacgttgt caaactcctc atcagagctc
     3001 agcccctctt gtttgagggc tgactcagca gctgagcggg aagctttccg ccaggcatcc
     3061 ttttccaagg atttgatccg ggcttggagc tgttcattga cctcatgctg ttcctccagt
     3121 tcccgctgtg ccttcttcct caggctgtcc agccgctcaa tttcctcttc tgcttcatcc
     3181 acctgccgct tcaaagcctt caccctcagg gttagctggt ccttctggtc gttgacatgc
     3241 tgccgttcat catcaatctg gatggagagc tccttaaccc tccgttctag tttgcgattg
     3301 gtggactgca gaactgtctt ctctctctct tcagcttgca gccgctcttg cagcagctga
     3361 ttctgggact caagctggga gaggctagcg ctgggtttct ggaagccctc tgaactggcc
     3421 aaccgggtct tcaaatcctt gttctgtctc tccaaggaga ttttgtcaca ctccaagtcc
     3481 tgtcgagcag atctctcctg catgagctct gtcctcagtt gatccacctg atcccggcca
     3541 cggttcaccc gatctgttaa cagttccaca gtgttctttt cttcatctaa ctccgcttcc
     3601 agccgcgaga ctttctcctc taggctcttc agctgtcgag ccttgtcgtc ctggaagcgt
     3661 ttcttgttct cagcctcttg ctctagaccc tgcagtcgct gggccagcag ctctttgtcc
     3721 agcctggctg tgtcccgctc agcctgggat gtctgcaggg cttgccgcag cctttgcagc
     3781 tcatcctgaa gccggctcag cccccctgaa ttcttttcag cctcactggc ccaatccttg
     3841 gcctgacgct gggcatcagc tacttccttc cgggcctttt ccttgtaatc ttccatctga
     3901 gcctggagct gctgcagggc tagcttggag tcatccccaa tctgctccag ctctttgttg
     3961 agtcggtcta cagtcctgtt cagcaaacgc ttctgctcct ccaactctgc cttgctcctc
     4021 cggagagcct cccgctgctt cccctcttcc tctagggccc ggttcagtgc ttgctgttcc
     4081 tgcccaaggc gtgccagccc ccgctgggcc tcatctagcc ggacctccag agcccgcttg
     4141 gctgctgcca ggttcccctc ctcctcccgg gcagcattca aagcctcttc cagctgctgt
     4201 ttctctacct ccagccgatg cactttgtcc cgaagccgtg cctccaccgc ttccccacct
     4261 tccgccagac ctcgggcctc cttcaactgc tgctccagac ccaggatgcg ccttcggaat
     4321 tcgtcatttt cctcttgtgt ctcccgaagt gtggtttcca cggttgcccg acgctgcccc
     4381 agcaccatgg cctctgtctc tgatgccacc ttagccttgg aagcctcttc acagtcctgc
     4441 cgcagttgct gcagggtctt ttgtagctgg aggttctgct gctccagctc tcggcctcga
     4501 tcagcctcct ccctcagcgc tgccagctgc ttctctaaca cctggttctg gtgtcgccca
     4561 gtcacctcct ggttctgctg ctctgcctga agctctttaa gctccccctg ggtgcgtcga
     4621 agctcctttt taagttcctc aacctggtga gtatctcttg tactggctcg ggcctgctct
     4681 aagtccctct gtaagacctc catcttctcc ccaagttcct cttccatctc ctccttctcc
     4741 atccgcagtt gcagcagttc ctgcttggtg gctctcagct cttccttgtt cttctggaac
     4801 atgctctgcc aatgccctgt ctcctcagag gtctcctcta gctctctttg cagctcccgc
     4861 acgagggaag acatcttctg cctctcagcc tccagtgcag catggtcctg ggtcgcatct
     4921 tgcatgctcc ggcgaagctg ctccgtgtct cgctggtact gcagccggac atgttccacc
     4981 tcctggtcgt gggaggccac ctcctctttc agggccccct tcagggctgt cagctcccgt
     5041 tccctcagcc tgagctgctc ctctactcgt tgtttgccct ctaagagttc ttctagaagt
     5101 tcccgggtgt ctaacaagtc ctttaggaga ctttcctttc cagaatcggg actctggcta
     5161 tgcttgagct taagctgtag ctccttcacc tgggcctcca gcccatgccg tagtccttct
     5221 tcctggccta gcaggagctt catgttctga agttccttgc tgctctgctg gacctccccc
     5281 tttctcctct ccagcaactc ctgcagtcgg tggcactctt cagccttctc ctccagctgc
     5341 ctctccagcc caaccctgga tggttctagc ttctgccgct tcttcacctc ctcatccagc
     5401 ttcttctgaa gttcctccat ttttcgggta agctcagcct gtcctgccag ggccttggta
     5461 gaagcaggag aaaccatccc cagaggctgc atttgctcca gcaccaaact aactttcctc
     5521 ctcacagatg cctcactctc cgagcttccc tcccttagga tgccatagat ggtggccttc
     5581 acatggtccg cactgcctgg tggcgctgtc tctctctggt ctctgagaag gtctggagtt
     5641 gatttgaact gcaccatggc tggatcccgt gtctcctcaa aactctgaag cacccagtct
     5701 tgagtctgac gcgaacggct gaagctgctg aacgggctct gcttctgcga tgggttcttt
     5761 gaactgtcca catggttgtc atactttgtg ccgcgggtcc agtggttagc ggactggtgc
     5821 tcccgttcct cacgtgtatc ccgtgggagc ctgctgtcca gactctggct ccgcttgcgc
     5881 tgttcgtggg gaagtgtcct tgtgcggcgc ccagtgcgac cccggacctg cccccccttc
     5941 tgactatcaa acttgttgat gagcgagtcc actgaagaca agggggcagt gtcaatggtg
     6001 ttaatcgagg aagtcggttt aggggctagt tccagcaagc tggtgctcct attactagga
     6061 ctcataaagg caagcccggt cagagaggct tgcgactggg aacggatcag ttttccattc
     6121 aagtggtcct tgggctcctc atcagatgtg ctgctccgag aagtggcagg acgtcccttg
     6181 acttgagagt aggggttttc ggggagctct gaatcggagc tcagtgcacc tggggacccc
     6241 cggttgttgc cccctttgat ctggacccca aaggagtcag tccctttctc tccactgtta
     6301 agaaccacaa acggttgccc ggcgatgccc tgcacacgca cagcgacccc ataggtactg
     6361 gctcttgcat cccttgcagg gcgccgccca ctacgacgaa gggtgcccat ttcagtagca
     6421 ccctctggct ctgtaataaa tcgaatttga actccatggt ctacggggcc ccggggatca
     6481 gccatcatgc ggccgctctt gtacagctcg tccatgccga gagtgatccc ggcggcggtc
     6541 acgaactcca gcaggaccat gtgatcgcgc ttctcgttgg ggtctttgct cagggcggac
     6601 tgggtgctca ggtagtggtt gtcgggcagc agcacggggc cgtcgccgat gggggtgttc
     6661 tgctggtagt ggtcggcgag ctgcacgctg ccgtcctcga tgttgtggcg gatcttgaag
     6721 ttcaccttga tgccgttctt ctgcttgtcg gccatgatat agacgttgtg gctgttgtag
     6781 ttgtactcca gcttgtgccc caggatgttg ccgtcctcct tgaagtcgat gcccttcagc
     6841 tcgatgcggt tcaccagggt gtcgccctcg aacttcacct cggcgcgggt cttgtagttg
     6901 ccgtcgtcct tgaagaagat ggtgcgctcc tggacgtagc cttcgggcat ggcggacttg
     6961 aagaagtcgt gctgcttcat gtggtcgggg tagcggctga agcactgcac gccgtaggtc
     7021 agggtggtca cgagggtggg ccagggcacg ggcagcttgc cggtggtgca gatgaacttc
     7081 agggtcagct tgccgtaggt ggcatcgccc tcgccctcgc cggacacgct gaacttgtgg
     7141 ccgtttacgt cgccgtccag ctcgaccagg atgggcacca ccccggtgaa cagctcctcg
     7201 cccttgctca ccatgaattc caccacactg gactagtgga tccgagctcg gtaccaagct
     7261 taagtttaaa cgctagccag cttgggtctc cctatagtga gtcgtattaa tttcgataag
     7321 ccagtaagca gtgggttctc tagttagcca gagagctctg cttatataga cctcccaccg
     7381 tacacgccta ccgcccattt gcgtcaatgg ggcggagttg ttacgacatt ttggaaagtc
     7441 ccgttgattt tggtgccaaa acaaactccc attgacgtca atggggtgga gacttggaaa
     7501 tccccgtgag tcaaaccgct atccacgccc attgatgtac tgccaaaacc gcatcaccat
     7561 ggtaatagcg atgactaata cgtagatgta ctgccaagta ggaaagtccc ataaggtcat
     7621 gtactgggca taatgccagg cgggccattt accgtcattg acgtcaatag ggggcgtact
     7681 tggcatatga tacacttgat gtactgccaa gtgggcagtt taccgtaaat actccaccca
     7741 ttgacgtcaa tggaaagtcc ctattggcgt tactatggga acatacgtca ttattgacgt
     7801 caatgggcgg gggtcgttgg gcggtcagcc aggcgggcca tttaccgtaa gttatgtaac
     7861 gcggaactcc atatatgggc tatgaactaa tgaccccgta attgattact attaataact
     7921 agtcaataat caatgtcaac gcgtatatct ggcccgtaca tcgcgaagca gcgcaaaacg
     7981 cctaacccta agcagattct tcatgcaatt gtcggtcaag ccttgccttg ttgtagctta
     8041 aattttgctc gcgcactact cagcgacctc caacacacaa gcagggagca gatactggct
     8101 taactatgcg gcatcagagc agattgtact gagagtgcac cataggggat cgggagatct
     8161 cccgatccgt cgacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt
     8221 ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg
     8281 cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt
     8341 cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta
     8401 aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc
     8461 ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa
     8521 gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc
     8581 cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt
     8641 acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact
     8701 gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac
     8761 aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata
     8821 ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta
     8881 ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg
     8941 gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat
     9001 aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt
     9061 aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga
     9121 aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa
     9181 gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag
     9241 gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac
     9301 tgagcgtcag accccgtaga aaagatcaaa ggatcttc
//
