LOCUS       sequence-469305-        9366 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of  hParacingulinL1   in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9366)
  AUTHORS   Vasileva E, Spadaro D, Rouaud F, King JM, Flinois A, Shah J, 
            Sluysmans S, Mean I, Jond L, Turner JR, Citi S
  TITLE     Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to 
            promote its extended conformation, stabilization, and tight junction
            accumulation.
  JOURNAL   J Biol Chem. 2022 Apr;298(4):101797. doi: 10.1016/j.jbc.2022.101797.
            Epub 2022 Mar 5.
  PUBMED    35259394
REFERENCE   2  (bases 1 to 9366)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-04"; volume: "298(4):101797. doi"; pages: " 
            10.1016/j.jbc.2022.101797. Epub 2022 Mar 5"
COMMENT     Sequence Label: hCGNL1-Myc-HA
FEATURES             Location/Qualifiers
     source          1..9366
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      119..547
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(206..225)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     416..437
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(556..576)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        561..890
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      741..876
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     803..822
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     CDS             957..1751
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1011..1030)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     1621..1640
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    1925..2046
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1962..1981)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2016..2035
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2095..2111)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2095..2111)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2108..2130)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2119..2135
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2143..2149,2150..2167,2168..2173))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2188..2209
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2326..2343)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2497..3085)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2577..2596)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3256..4047,4048..4116))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3879..3898
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(4117..4221)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4296..4315)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4487..4866
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4867..5070
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5021..5041
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     5115..5134
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        5115..5133
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      5187..5196
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             9105..9134
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             9138..9164
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     primer_bind     complement(9209..9226)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    join(9215..9366,1..73)
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
       61 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
      121 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
      181 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
      241 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
      301 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
      361 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
      421 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
      481 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
      541 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
      601 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
      661 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
      721 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
      781 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
      841 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
      901 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
      961 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     1021 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     1081 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     1141 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     1201 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     1261 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     1321 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     1381 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     1441 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     1501 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     1561 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     1621 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     1681 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     1741 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     1801 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     1861 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     1921 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     1981 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     2041 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     2101 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     2161 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     2221 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     2281 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     2341 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     2401 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     2461 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     2521 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     2581 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     2641 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     2701 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     2761 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     2821 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     2881 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     2941 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     3001 ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat
     3061 tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc
     3121 tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt
     3181 cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta
     3241 aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct
     3301 atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg
     3361 cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga
     3421 tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt
     3481 atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt
     3541 taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt
     3601 tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat
     3661 gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc
     3721 cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc
     3781 cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat
     3841 gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag
     3901 aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt
     3961 accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc
     4021 ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa
     4081 gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg
     4141 aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa
     4201 taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc
     4261 gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4321 ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa
     4381 atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt
     4441 aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg
     4501 actagttatt aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc
     4561 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     4621 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     4681 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     4741 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     4801 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     4861 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     4921 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     4981 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     5041 gtacggtggg aggtctatat aagcagagct ctctggctaa ctagagaacc cactgcttac
     5101 tggcttatcg aaattaatac gactcactat agggagaccc aagctggcta gcgtttaaac
     5161 ttaagcttgg taccgagctc ggatccgcca ccatggagct gtatttcggt gaatatcaac
     5221 atgtgcagca ggaatatggg gtccatctga gactcgcaag tgatgatacc caaaaatcaa
     5281 ggagttccca gaactccaag gcaggctcct acggtgtcag tattcgggtc cagggaattg
     5341 atggtcaccc ctatattgtc ctgaataaca cagaacggtg cctagcaggc acatcgtttt
     5401 ctgaaaatgg gccacccttt ccacctccag tgataaataa cctgcctcta cattccagca
     5461 atggttctgt gccaaaggag aacagtgaag aacttcagct tccagaaaac ccatacgccc
     5521 agcctagccc aataagaaac ctgaaacagc ccctgctcca tgagggcaag aatggagttc
     5581 tagatcgcaa agacgggtct gtgaagccat ctcacctgct gaactttcag aggcatccag
     5641 agcttttgca accctatgac cctgaaaaga atgagttgaa tttacaaaat caccagcctt
     5701 ctgagagtaa ttggctaaaa acgttgacag aagaaggcat caacaataag aagccttgga
     5761 cttgctttcc caaacctagc aattcccaac ctaccagtcc ctccttggaa gacccggcca
     5821 aatctggtgt gacagctatt cgtttatgca gctccgtggt catagaggac cccaaaaagc
     5881 agacctcagt gtgtgtaaac gttcagggct gcaccaagga gagggtggga gaggaggccc
     5941 ttttcactag cgggaggccc ctgactgccc acagcccaca tgcccaccct gaaaccaaga
     6001 aaaccaggcc agatgttctt cccatccggc gacaggattc agcgggaccc gtcctggatg
     6061 gagctcggtc ccggaggtcc tcctcgtcat ccacaactcc cacgccagcc aactctttgt
     6121 acaggttttt actggatgat caggaatgtg ccatccatgc cgacaacgtc aatcgtcatg
     6181 aaaacagaag gtatattccc ttcctgccag gaactggacg ggatattgat acaggatcaa
     6241 ttcctggtgt ggatcagtta attgaaaaat ttgatcaaaa acctgggctt cagagaagag
     6301 gaaggtctgg gaagcgaaac agaattaatc cagatgacag gaaaagatcc agaagcgtgg
     6361 atagcgcctt tccttttggc ctccaaggga actcggagta cctgattgaa ttcagtagga
     6421 acttgggcaa gtcaagcgaa cacctcctcc ggccttccca ggtgtgcccg cagcggccac
     6481 tgtctcagga gcgccgtggg aaacagagcg tgggccgcac ctttgcaaag ctgcagggag
     6541 cagcgcacgg ggcttcatgt gcccactcca ggcctcccca gccgaacata gatgggaaag
     6601 ttctggaaac cgaaggtagt caggaaagta cagtgatccg tgcgccctcc cttggtgcac
     6661 agagtaaaaa ggagggggag gtgaaaacag ccaccgctac gctgatgtta cagaaccggg
     6721 cagcagcaac ttcgcctgat tctggtgcca agaaaatttc cgtgaagaca tttccttcgg
     6781 cctcaaatac tcaggccaca ccggatctct taaagggcca gcaagagctc actcagcaaa
     6841 ccaatgagga gacagctaag cagattctct acaattacct caaagaagga agcactgata
     6901 atgacgatgc tactaaaagg aaagtcaact tggtctttga gaaaatccag accttaaagt
     6961 ctcgagcagc tgggagcgcc caaggaaata accaagcttg taattccaca tctgaagtca
     7021 aagatctatt ggaacagaaa agcaagttga ccatagaagt ggctgaactt cagagacagc
     7081 ttcaactgga agtcaagaat caacggaaca ttaaagaaga gagagagagg atgagagcaa
     7141 acctagaaga gctccgaagc caacacaacg aaaaggtgga ggagaactcc acattgcagc
     7201 aacgactgga agaaagtgaa ggggagctcc ggaagaatct ggaggagcta ttccaggtga
     7261 agatggaacg ggagcagcat cagactgaga tcagggatct ccaggaccag ccctcagaaa
     7321 tgcacgatga actggacagt gcaaagcgat cggaggacag ggagaaggga gctctgattg
     7381 aggagctctt acaggcaaaa caggatcttc aagatctgct gattgccaaa gaggagcaag
     7441 aagacctctt gagaaagcga gagcgtgaac tcaccgccct gaagggagcc ctgaaagaag
     7501 aggtttccag ccatgatcag gagatggaca agctgaagga gcaatatgat gctgagttgc
     7561 aggccctgag ggagagtgtg gaagaagcaa ccaagaatgt cgaggtcttg gcgagcagga
     7621 gcaacacttc agagcaagac caggcgggga ctgaaatgcg cgtgaagctt ctgcaggagg
     7681 agaatgagaa gctgcaggga agaagcgaag agctggagcg gagagttgct cagcttcaaa
     7741 ggcagatcga ggacctgaaa ggcgatgaag ccaaggcgaa ggaaacgctg aagaagtacg
     7801 agggagaaat acgacagtta gaggaggccc ttgtgcacgc cagaaaggaa gaaaaagaag
     7861 ctgtgtcagc cagaagggcc ctggagaatg aactggaggc tgctcaggga aatctgagtc
     7921 agactaccca ggagcagaag cagttgtctg agaagctcaa agaggagagt gagcagaagg
     7981 agcagctaag aaggttgaag aacgagatgg agaatgagcg gtggcacctg ggcaaaacca
     8041 ttgagaaact gcagaaggag atggcagaca ttgttgaggc ctcccgtacc tcaaccctgg
     8101 agctccagaa ccagctggat gagtataagg agaaaaaccg cagggagctc gcagaaatgc
     8161 aaagacagtt gaaggagaaa acgctggagg cagaaaagtc ccgactgaca gccatgaaaa
     8221 tgcaggatga gatgcgtctg atggaggaag agttacggga ctaccagaga gctcaggatg
     8281 aagcactcac aaaaaggcag cttctggagc agacgctgaa ggacctggag tatgagctgg
     8341 aagccaagag tcacctcaaa gatgaccgca gcaggctggt caagcagatg gaggccaagg
     8401 tgtctcaact ggagatggaa ctggaagaag agagaaacaa ctcagatttg ctgtctgaga
     8461 ggatcagtag gagcagggaa cagatggagc agttgaggaa tgggctactt caggagagag
     8521 ctgcgagaca agacttggag tgcggcaaga tttccctgga gaggcagaac aaggacttaa
     8581 agagccggat tatccacctg gaaggttcct acaggtccag caaagagggg ctggttgtgc
     8641 agatggaggc caggatcgcg gagctggagg accgcctgga gagtgaggag agggatcggg
     8701 ccaatcttca gctcagcaac cggcggctgg agcggaaagt gaaggagctg gtgatgcagg
     8761 tggatgatga gcacctgtca ttgactgatc agaaggacca gctgagcttg cgtttgaaag
     8821 ccatgaagcg gcaggtggag gaggctgagg aggaaatcga cagactggaa agttctaaaa
     8881 agaagctgca gagggagctg gaggagcaga tggacatgaa tgagcatctg caggggcagc
     8941 tcaactccat gaagaaggac ttaagactga agaagctgcc gagtaaagtg ctggatgaca
     9001 tggatgacga cgatgacctc agcacggatg ggggaagcct ctatgaggcg cctgtgagct
     9061 acacattctc caaggacagc accgtcgcca gccagatcgg taccgagcag aagctgatct
     9121 cagaggagga cctggagtac ccatacgacg taccagatta cgctgcggcc gctcgagtct
     9181 agagggcccg tttaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     9241 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     9301 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     9361 ggtggg
//
