LOCUS       sequence-469308-        9807 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  For expression of Cingulin (human) in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9807)
  AUTHORS   Vasileva E, Spadaro D, Rouaud F, King JM, Flinois A, Shah J, 
            Sluysmans S, Mean I, Jond L, Turner JR, Citi S
  TITLE     Cingulin binds to the ZU5 domain of scaffolding protein ZO-1 to 
            promote its extended conformation, stabilization, and tight junction
            accumulation.
  JOURNAL   J Biol Chem. 2022 Apr;298(4):101797. doi: 10.1016/j.jbc.2022.101797.
            Epub 2022 Mar 5.
  PUBMED    35259394
REFERENCE   2  (bases 1 to 9807)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2022-04"; volume: "298(4):101797. doi"; pages: " 
            10.1016/j.jbc.2022.101797. Epub 2022 Mar 5"
COMMENT     Sequence Label: GFP-hCGN
FEATURES             Location/Qualifiers
     source          1..9807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      1..589
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     490..509
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     743..760
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    877..898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(913..918,919..936,937..943)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    951..967
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     956..978
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     975..991
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     975..991
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     polyA_signal    1040..1161
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1051..1070)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     1105..1124
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     CDS             complement(1335..2129)
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(1446..1465)
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     2056..2075
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     promoter        complement(2196..2525)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2210..2345
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2264..2283)
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     2510..2530
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     rep_origin      complement(2539..2967)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2649..2670)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2861..2880
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     polyA_signal    3013..3237
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     3226..3243
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(3287..3304)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(3320..3349)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     RBS             5586..5594
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     CDS             complement(join(6986..7696,7697..7699,7700..7702))
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             complement(join(6986..7696,7697..7699,7700..7702))
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7028..7049)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     7377..7396
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7636..7657
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     regulatory      7699..7708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(7710..7726)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7710..7726)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(7759..7778)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7760..7778)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(7823..8026)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(7852..7872)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        8027..8406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     8578..8597
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        8672..8776
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8777..8845,8846..9637)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8995..9014)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc
       61 agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt
      121 cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt
      181 caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc
      241 tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa
      301 ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac
      361 ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg
      421 gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga
      481 gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact
      541 tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa
      601 cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc
      661 gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg
      721 ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat
      781 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
      841 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      901 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      961 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctct agctagaggt
     1021 cgacggtata cagacatgat aagatacatt gatgagtttg gacaaaccac aactagaatg
     1081 cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatt
     1141 ataagctgca ataaacaagt tggggtgggc gaagaactcc agcatgagat ccccgcgctg
     1201 gaggatcatc cagccggcgt cccggaaaac gattccgaag cccaaccttt catagaaggc
     1261 ggcggtggaa tcgaaatctc gtgatggcag gttgggcgtc gcttggtcgg tcatttcgaa
     1321 ccccagagtc ccgctcagaa gaactcgtca agaaggcgat agaaggcgat gcgctgcgaa
     1381 tcgggagcgg cgataccgta aagcacgagg aagcggtcag cccattcgcc gccaagctct
     1441 tcagcaatat cacgggtagc caacgctatg tcctgatagc ggtccgccac acccagccgg
     1501 ccacagtcga tgaatccaga aaagcggcca ttttccacca tgatattcgg caagcaggca
     1561 tcgccatggg tcacgacgag atcctcgccg tcgggcatgc gcgccttgag cctggcgaac
     1621 agttcggctg gcgcgagccc ctgatgctct tcgtccagat catcctgatc gacaagaccg
     1681 gcttccatcc gagtacgtgc tcgctcgatg cgatgtttcg cttggtggtc gaatgggcag
     1741 gtagccggat caagcgtatg cagccgccgc attgcatcag ccatgatgga tactttctcg
     1801 gcaggagcaa ggtgagatga caggagatcc tgccccggca cttcgcccaa tagcagccag
     1861 tcccttcccg cttcagtgac aacgtcgagc acagctgcgc aaggaacgcc cgtcgtggcc
     1921 agccacgata gccgcgctgc ctcgtcctgc agttcattca gggcaccgga caggtcggtc
     1981 ttgacaaaaa gaaccgggcg cccctgcgct gacagccgga acacggcggc atcagagcag
     2041 ccgattgtct gttgtgccca gtcatagccg aatagcctct ccacccaagc ggccggagaa
     2101 cctgcgtgca atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca
     2161 gatccgaaaa tggatataca agctcccggg agctttttgc aaaagcctag gcctccaaaa
     2221 aagcctcctc actacttctg gaatagctca gaggcagagg cggcctcggc ctctgcataa
     2281 ataaaaaaaa ttagtcagcc atggggcgga gaatgggcgg aactgggcgg agttaggggc
     2341 gggatgggcg gagttagggg cgggactatg gttgctgact aattgagatg catgctttgc
     2401 atacttctgc ctgctgggga gcctggggac tttccacacc tggttgctga ctaattgaga
     2461 tgcatgcttt gcatacttct gcctgctggg gagcctgggg actttccaca ccctaactga
     2521 cacacattcc acagaattaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc
     2581 aataggccga aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga
     2641 gtgttgttcc agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag
     2701 ggcgaaaaac cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt
     2761 ttttggggtc gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta
     2821 gagcttgacg gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag
     2881 cgggcgctag ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg
     2941 cgcttaatgc gccgctacag ggcgcgtggg gataccccct agagccccag ctggttcttt
     3001 ccgcctcaga agccatagag cccaccgcat ccccagcatg cctgctattg tcttcccaat
     3061 cctccccctt gctgtcctgc cccaccccac cccccagaat agaatgacac ctactcagac
     3121 aatgcgatgc aatttcctca ttttattagg aaaggacagt gggagtggca ccttccaggg
     3181 tcaaggaagg cacgggggag gggcaaacaa cagatggctg gcaactagaa ggcacagtcg
     3241 aggctgatca gcggtttaaa cttaagctgg agaccgttta aactcaatga tgatgatgat
     3301 gatggtcgac ggcgctattc agatcctctt ctgagatgag tttttgttcg ggcccaagct
     3361 tggtaccaca ggagctggcc tgtaggttgc tctccgtaag cagtgatgca atggacgagg
     3421 gatcgtagac actgtcgaat tcctcatctg agctcagccc ttcgtttttg agagctgact
     3481 cagcagctga gcgggaagct ttgcgccagg agtccttctc cagagacttg atccgggcct
     3541 ggagctgttc attgacctca tgctgctcct ccacctcacg ctgggccttc ttcctcaggc
     3601 cgtccagtcg ctcaatttcc tcttctgctt catccacctg acgcttcaaa gccttcaccc
     3661 tcaggcttag ctggtctttc tggtcattga catgctgccg ctcgtcttca atctggatgg
     3721 atagttcttt aactttccgc tccagttttc gattggtaga ctgcagaact gtcttctccc
     3781 tctcttcagc ctgtagccgc tcctgcaaca actgattctg ggactcaagc tgagagaggc
     3841 tggcactagg cttctggaag ccttctgagc tggccaaccg ggtcttcagg tccttgttct
     3901 gtctctccaa ggagattttg tcacactcca ggtcctgccg agcagacctt tcctgcatga
     3961 gctctgtcct cagctgatcc acctggtccc ggccacgatt cacccgatct gttagcagct
     4021 ccacggtgtt cttctcctca tctaactctg tttccagccg tgagactttt tcctcgagac
     4081 ccttcagctg ccgggccctg tcgtcctggg aacgcttctt gttctctgcc tcttgctcca
     4141 gcccctgcag tcgctgggcc agtagctctt tgtccagccg ggctgtgtcc cgctcagcct
     4201 gggatgcctg cagggcctgc cgcagcctct ggatctcatc ctgaagtcgg ctcagtcctc
     4261 cagaggtctt ctcagcctca ctggcccaat ccttggcctg gcgctgggca tctgccacct
     4321 cccgccgggc cttttcctta taatcctcca gctgggcctg gagctgctgc agggcttgct
     4381 tagagtcctc cccgatcttc tccaactcct tgttcagtcg gtccacagtc ctgtccagca
     4441 aacgcttctg ctcctccagc tcagccttgc ctcgccggag cacctcccgc tgcttccctt
     4501 cctcctccag ggcccggttc agtgtctgct gctcctgccc caggcgggcc agcccccgct
     4561 gagcctcctc taggcgtgcc tccagtgccc gcttggctgc tgccagactc ccctcctctt
     4621 cctgggacgc attcagggcc tcctccagct gctgtttctc tgcctccagc cgctgcagct
     4681 tgtcccgtag tcgtgcctcc accgcttccc caccatccac cagacctcga gtctccttca
     4741 gctgctgctc caaacccagg atgcgccggc ggaattcgtc attttcctcc tgggtctccc
     4801 gaagcgtcgt ctccactgcg gcccgccgct gccccagcac tgttgcctct gcctcggcca
     4861 ccatcttagc cttggaagcc tcttcacagt cctgtcgcag ttgctggagg gtcttttgta
     4921 gctggaggtt ctgttcttcc agctcccgac ctcgatcagc ctcgaccctc aggaccgcca
     4981 gctgcttctc caactcccgg tcccggtgtc gcccagccac ctcctggctc tgccgttctg
     5041 cctgcagttc cttaagctcc tcctgtgtcc ggagcagctc cttcttgagc acctcaacct
     5101 ggcgagtatc tccagcacta gctcgggcct gctctaattc cctctgcaag acctctatct
     5161 tctctccaag ctcctcttcc atctcctcct tctccattcg cagctgcagg agttcctgct
     5221 tggtggctct aagatcctcc ttgttcttct ggaacatact ctgccaatgc cctgtctcct
     5281 ctgaagtctc ctccagctcc ctctgcagcc ctcgcacaag ggctgacatc ttctgcctct
     5341 cggcctccag cactgcatgg tcctgggttg catcttgcat gctcctgcgg agctgctctg
     5401 tgtctcgctg gtactgctgc cggacatgtt ccacctcctg gtcacgggag gctacctcct
     5461 ctttcagggc ccccttcagg gctgtcaact cccgctcccg cagcctcagc tgctcctcta
     5521 ctcgctgttt cccctccaag acctcttcca gaagttcccg ggtctctaac aggtccttca
     5581 gtaacacctc cttagcaggc tcaggaccct ggacatgttt cagcttgttc tgcagctcca
     5641 tcacctgggt ctccagccca tgtcgtaaat cttcaccctg gtccaagagg cgcttcatgt
     5701 tctggagctc cttgttgctc tgctgggcct cccccttcct cctctccagc agctcctgca
     5761 gtcggctgca ctcttctgtt ttctcctcca gctgccgctc cagcccaact tgggatggct
     5821 ctagcttctg ccgcttcttc acctcttcat ccagctttcg ctgtagctcc tccacttttc
     5881 gggtaagctc accctgccct gccacggcct tagtagaacc agaagaaacc atcactagag
     5941 gctgcatctt ctccagcacc aaactaacct tcctcctcac agaggtttca ctttctgagc
     6001 ttccctccct caggatgcca tagatggtgg ccttcatatg gtccacactg cctggtgggg
     6061 ctgcctcctg ctggtctcga aggaggtctg gagttgattt gaactgcagc atggtggggt
     6121 cctgtgcact cctccgcggc tcctcaaaac tctgaaggac ccagtcctga gtctgacgag
     6181 aacggctaaa gccactgaga ggactcaggt tctggctctg ggctggctgc ttcgaagtgc
     6241 ccacatggtt gtcatatttt gtgctagagg tccagtggtt ggtggactgg cgctcccgtt
     6301 cctcaaaggt gtcccgtggg aggcggctgt ccaggctctt gctccgtttg cgctgttcag
     6361 ggggtagcat ccgtgttcgg cggccagtcc gaccccgggc ctggcctcca agttgactgt
     6421 caaacttgtt gatgagtgag tccactgaag acaggggagc agtgtcaatg gtgctacctg
     6481 gggaagccac tttcggggct agctccagca tgctgttgct tctgttacta ggatccactg
     6541 ggccagggcc tgccagtgag gcctgggagt gggaacggag tagctttcca ttccagtagg
     6601 ccccaggctc ctcatcagat gtgctgctct gcgagggggc aggaaatccc ttgacctgag
     6661 agtaggggtt ctcagggagt tccaaatctg agctcagagc tcctgaggcc ccttggtcat
     6721 tggccccctt gatttggacc ccaaaggagt caccgccttt ctccccactg ttgagcacca
     6781 caaagggctg cccagcgatt ccctgcacac gcacagcaac cccgtaggta ctggctcttg
     6841 catccttagc tgggcgtcgt ccacctcgac gtagagtgcc catctctgca ccactcactg
     6901 gctctgtgat gaagcgaatc tggactccat ggtctacggg gccccggggc tcagccatgt
     6961 tgggtgcctg ctccatgcgg ccgctcttgt acagctcgtc catgccgaga gtgatcccgg
     7021 cggcggtcac gaactccagc aggaccatgt gatcgcgctt ctcgttgggg tctttgctca
     7081 gggcggactg ggtgctcagg tagtggttgt cgggcagcag cacggggccg tcgccgatgg
     7141 gggtgttctg ctggtagtgg tcggcgagct gcacgctgcc gtcctcgatg ttgtggcgga
     7201 tcttgaagtt caccttgatg ccgttcttct gcttgtcggc catgatatag acgttgtggc
     7261 tgttgtagtt gtactccagc ttgtgcccca ggatgttgcc gtcctccttg aagtcgatgc
     7321 ccttcagctc gatgcggttc accagggtgt cgccctcgaa cttcacctcg gcgcgggtct
     7381 tgtagttgcc gtcgtccttg aagaagatgg tgcgctcctg gacgtagcct tcgggcatgg
     7441 cggacttgaa gaagtcgtgc tgcttcatgt ggtcggggta gcggctgaag cactgcacgc
     7501 cgtaggtcag ggtggtcacg agggtgggcc agggcacggg cagcttgccg gtggtgcaga
     7561 tgaacttcag ggtcagcttg ccgtaggtgg catcgccctc gccctcgccg gacacgctga
     7621 acttgtggcc gtttacgtcg ccgtccagct cgaccaggat gggcaccacc ccggtgaaca
     7681 gctcctcgcc cttgctcacc atggtggcgg atccactagt tctagagggc ccgtttaaac
     7741 gctagccagc ttgggtctcc ctatagtgag tcgtattaat ttcgataagc cagtaagcag
     7801 tgggttctct agttagccag agagctctgc ttatatagac ctcccaccgt acacgcctac
     7861 cgcccatttg cgtcaatggg gcggagttgt tacgacattt tggaaagtcc cgttgatttt
     7921 ggtgccaaaa caaactccca ttgacgtcaa tggggtggag acttggaaat ccccgtgagt
     7981 caaaccgcta tccacgccca ttgatgtact gccaaaaccg catcaccatg gtaatagcga
     8041 tgactaatac gtagatgtac tgccaagtag gaaagtccca taaggtcatg tactgggcat
     8101 aatgccaggc gggccattta ccgtcattga cgtcaatagg gggcgtactt ggcatatgat
     8161 acacttgatg tactgccaag tgggcagttt accgtaaata ctccacccat tgacgtcaat
     8221 ggaaagtccc tattggcgtt actatgggaa catacgtcat tattgacgtc aatgggcggg
     8281 ggtcgttggg cggtcagcca ggcgggccat ttaccgtaag ttatgtaacg cggaactcca
     8341 tatatgggct atgaactaat gaccccgtaa ttgattacta ttaataacta gtcaataatc
     8401 aatgtcaacg cgtatatctg gcccgtacat cgcgaagcag cgcaaaacgc ctaaccctaa
     8461 gcagattctt catgcaattg tcggtcaagc cttgccttgt tgtagcttaa attttgctcg
     8521 cgcactactc agcgacctcc aacacacaag cagggagcag atactggctt aactatgcgg
     8581 catcagagca gattgtactg agagtgcacc ataggggatc gggagatctc ccgatccgtc
     8641 gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     8701 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     8761 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     8821 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     8881 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     8941 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     9001 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     9061 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     9121 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     9181 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     9241 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     9301 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     9361 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     9421 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     9481 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     9541 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     9601 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     9661 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     9721 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     9781 ccccgtagaa aagatcaaag gatcttc
//
